##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_34.34100000006a3a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 59971 Sequences flagged as poor quality 0 Sequence length 51 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.3418485601374 38.0 38.0 38.0 32.0 38.0 2 36.36087442263761 38.0 38.0 38.0 32.0 38.0 3 36.350219272648445 38.0 38.0 38.0 32.0 38.0 4 36.37493121675477 38.0 38.0 38.0 32.0 38.0 5 36.43089159760551 38.0 38.0 38.0 32.0 38.0 6 38.32120524920378 40.0 38.0 40.0 38.0 40.0 7 38.20173083657101 40.0 38.0 40.0 38.0 40.0 8 38.214837171299465 40.0 38.0 40.0 38.0 40.0 9 38.17785262877057 40.0 38.0 40.0 38.0 40.0 10 38.13371462873722 40.0 38.0 40.0 38.0 40.0 11 38.09071051007987 40.0 38.0 40.0 38.0 40.0 12 37.985342915742606 40.0 38.0 40.0 32.0 40.0 13 37.99598139100565 40.0 38.0 40.0 32.0 40.0 14 37.980523919894615 40.0 38.0 40.0 32.0 40.0 15 37.97045238531957 40.0 38.0 40.0 32.0 40.0 16 37.9778559637158 40.0 38.0 40.0 32.0 40.0 17 37.98560971136049 40.0 38.0 40.0 32.0 40.0 18 38.02076003401644 40.0 38.0 40.0 32.0 40.0 19 38.06034583381968 40.0 38.0 40.0 38.0 40.0 20 38.03890213603241 40.0 38.0 40.0 32.0 40.0 21 38.092161211252105 40.0 38.0 40.0 38.0 40.0 22 38.11045338580314 40.0 38.0 40.0 38.0 40.0 23 38.06616531323473 40.0 38.0 40.0 38.0 40.0 24 38.05854496339898 40.0 38.0 40.0 32.0 40.0 25 38.04570542428841 40.0 38.0 40.0 32.0 40.0 26 37.99381367661036 40.0 38.0 40.0 32.0 40.0 27 37.990628803921894 40.0 38.0 40.0 32.0 40.0 28 37.89453235730603 40.0 38.0 40.0 32.0 40.0 29 37.91354154508012 40.0 38.0 40.0 32.0 40.0 30 37.870737523136185 40.0 38.0 40.0 32.0 40.0 31 37.85342915742609 40.0 38.0 40.0 32.0 40.0 32 37.850744526521154 40.0 38.0 40.0 32.0 40.0 33 37.794784145670405 40.0 38.0 40.0 32.0 40.0 34 37.82211402177719 40.0 38.0 40.0 32.0 40.0 35 37.81622784345767 40.0 38.0 40.0 32.0 40.0 36 37.76000066698904 40.0 38.0 40.0 32.0 40.0 37 37.773657267679376 40.0 38.0 40.0 32.0 40.0 38 37.751313134681766 40.0 38.0 40.0 32.0 40.0 39 37.68489770055527 40.0 38.0 40.0 32.0 40.0 40 37.642043654432975 40.0 38.0 40.0 32.0 40.0 41 37.66183655433459 40.0 38.0 40.0 32.0 40.0 42 37.626119290990644 40.0 38.0 40.0 32.0 40.0 43 37.62023311267112 40.0 38.0 40.0 32.0 40.0 44 37.565239865935204 40.0 38.0 40.0 32.0 40.0 45 37.521435360424206 40.0 38.0 40.0 32.0 40.0 46 37.43025795801304 40.0 38.0 40.0 32.0 40.0 47 37.383585399609814 40.0 38.0 40.0 32.0 40.0 48 37.299444731620284 40.0 38.0 40.0 32.0 40.0 49 37.2691801037168 40.0 38.0 40.0 32.0 40.0 50 37.21457037568158 40.0 38.0 40.0 32.0 40.0 51 36.85436294208868 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.1776748619136228 2201 2 -0.2727357173317273 2201 3 0.39131149852205027 2201 4 0.12109760695786065 2201 5 -0.6536050219736751 2201 6 0.22887705359978838 2201 7 0.03402272699593567 2201 8 0.025274066202797485 2201 9 0.2776706814151879 2201 10 0.1246037010480805 2201 11 0.11287190737043318 2201 12 0.05669385506871549 2201 13 -0.15116211242209232 2201 14 -0.19313309513146493 2201 15 0.11628610069443113 2201 16 -0.23206177948649298 2201 17 0.137119510221261 2201 18 -0.18583035708256546 2201 19 0.1294705902204143 2201 20 -0.22938716055688246 2201 21 -1.3203266856143188 2201 22 -0.16070369377921878 2201 23 -0.1662570431501038 2201 24 -0.7282202771301556 2201 25 -0.723454310293306 2201 26 -0.7792153942450213 2201 27 0.0652666440111318 2201 28 -1.2524381154267772 2201 29 -0.7246896395179405 2201 30 -0.830455577232641 2201 31 0.3021407954914608 2201 32 -0.8271516718295828 2201 33 -0.7313895560376693 2201 34 -0.3144132741847656 2201 35 -0.5243690159340701 2201 36 -1.0538405768253725 2201 37 -0.6633649699114059 2201 38 -0.4916899161538737 2201 39 -0.38348174362246823 2201 40 -0.46304373926263764 2201 41 0.21331516407413886 2201 42 -0.0710978140874019 2201 43 -0.06464494757219796 2201 44 0.13346446754964347 2201 45 0.1061596214663254 2201 46 0.09451376842087456 2201 47 -0.2547175550581855 2201 48 -0.503285046393664 2201 49 0.01540981590528645 2201 50 -0.648892108606347 2201 51 0.3268185900569165 1101 1 0.24044542468418229 1101 2 0.16990497530896675 1101 3 -0.12600451879396957 1101 4 -0.6960019601417073 1101 5 -0.32676519513384505 1101 6 -0.23649091176817905 1101 7 -0.8317781388049283 1101 8 0.041507832436565195 1101 9 -0.1985197947752937 1101 10 0.17979850624288218 1101 11 0.29360783810636093 1101 12 0.06318736156222116 1101 13 -0.6706426319026093 1101 14 -0.09897725097562216 1101 15 -0.2906403062319711 1101 16 -0.5946158920406077 1101 17 -0.91049953739779 1101 18 -0.11981304106524959 1101 19 -0.39650343575360836 1101 20 -0.18609711726683997 1101 21 0.1980715827839532 1101 22 -0.2851625682380927 1101 23 -0.8946120215050826 1101 24 0.11160656269668578 1101 25 0.7971084602694631 1101 26 -0.5422024072320326 1101 27 -0.5169844382399447 1101 28 0.0722372092485486 1101 29 0.2244445596162592 1101 30 0.5699773232002556 1101 31 -0.15456916121849673 1101 32 0.33410373942582794 1101 33 0.04350221885410832 1101 34 -0.10120981098130244 1101 35 -0.04384953541459424 1101 36 0.13447111148632018 1101 37 -0.5670446235910589 1101 38 0.20960878514483028 1101 39 -0.4137847739254994 1101 40 -0.1080653842842807 1101 41 -0.3765116757527025 1101 42 0.27630478331519726 1101 43 0.34444596151871565 1101 44 -0.3221632380780619 1101 45 -0.7520654867587808 1101 46 -0.43687151296440163 1101 47 -0.35212015246078465 1101 48 -0.7965750896837065 1101 49 -1.3233347728393028 1101 50 -1.465991675705915 1101 51 -0.3073805441422124 2202 1 0.2642549484937078 2202 2 0.7651430705470617 2202 3 0.38590024311079674 2202 4 0.6849504208106794 2202 5 0.5422824239137753 2202 6 0.09684242156514955 2202 7 0.40631709929031246 2202 8 0.1367459276746601 2202 9 0.18243258617709301 2202 10 0.5607508871952618 2202 11 0.8174173619158864 2202 12 1.1822349806098416 2202 13 0.8888811776212009 2202 14 1.0319751299767574 2202 15 0.5545977890061238 2202 16 0.5720507746260637 2202 17 0.5299766530783998 2202 18 0.6897107684585606 2202 19 0.27016323091305594 2202 20 0.8019981208284008 2202 21 0.8528334875458583 2202 22 0.5600755270000022 2202 23 0.42681654992348683 2202 24 0.5044637055538317 2202 25 0.7613941745551784 2202 26 0.3625594975298725 2202 27 0.12587270461719413 2202 28 0.3817610187723588 2202 29 0.5339683691400694 2202 30 0.3437868470097811 2202 31 0.881145124495788 2202 32 0.7031513584734483 2202 33 0.4006450759969624 2202 34 0.18450447473298226 2202 35 0.7775790360139823 2202 36 0.8011377781529845 2202 37 0.278193471647036 2202 38 0.4834183089543558 2202 39 1.4195485594078363 2202 40 1.2371727109538142 2202 41 0.8258692766282536 2202 42 0.5382095452199565 2202 43 0.36825548532823404 2202 44 0.1778367619219381 2202 45 1.0098392751459784 2202 46 0.646461820368927 2202 47 0.6954988951582664 2202 48 0.1081868150781986 2202 49 -0.4066681061726385 2202 50 0.6173416576274207 2202 51 -0.09309482985650419 1102 1 0.4861164203551809 1102 2 -0.4464370160330233 1102 3 0.2598179920285446 1102 4 0.1470716329318904 1102 5 -0.4344491778178323 1102 6 -0.04872034899761957 1102 7 -0.14454870157548783 1102 8 0.28014419607293206 1102 9 -0.11680983806533618 1102 10 -0.09292876648439119 1102 11 -0.010504716006195736 1102 12 -0.20142302804816836 1102 13 0.28227944601947286 1102 14 0.6023214503230818 1102 15 -0.0574151980068649 1102 16 -0.261282558707272 1102 17 0.2783532764550216 1102 18 0.7281306818784756 1102 19 0.1310939668437925 1102 20 -0.10925729042701704 1102 21 -0.03623577652340515 1102 22 0.12176383868830953 1102 23 1.0869897100966455 1102 24 0.4893121904023161 1102 25 0.5882340013950014 1102 26 -0.5438257838554108 1102 27 0.23085105959555108 1102 28 0.3222372092485486 1102 29 -0.042871457699753535 1102 30 0.4812327344556735 1102 31 0.5505174188680826 1102 32 0.03973144505353332 1102 33 0.21828576863765647 1102 34 0.31708023230874005 1102 35 -0.12826511983017497 1102 36 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31 -0.17421779737198761 1213 32 -0.7949993581238672 1213 33 -0.24960920047090696 1213 34 -0.6852366255906475 1213 35 -2.0077885090623013 1213 36 -2.133906142327831 1213 37 -2.550516653179592 1213 38 -1.9554397585444931 1213 39 -1.147950284790042 1213 40 -1.2532341313947768 1213 41 -1.452679035900644 1213 42 -0.8981057576001987 1213 43 -0.8559701272468914 1213 44 -0.8430832565708997 1213 45 -0.5822781543500923 1213 46 -0.18224830445770124 1213 47 -0.8701506656369276 1213 48 -1.4152759680933187 1213 49 -1.1535474404306143 1213 50 -0.7120619484752169 1213 51 0.21296157328728071 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 2.0 15 0.0 16 0.0 17 3.0 18 5.0 19 3.0 20 4.0 21 17.0 22 22.0 23 48.0 24 104.0 25 155.0 26 281.0 27 415.0 28 530.0 29 647.0 30 830.0 31 1018.0 32 1361.0 33 1619.0 34 2035.0 35 2521.0 36 3396.0 37 4905.0 38 9455.0 39 30595.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.71583003853661 25.891263366865186 14.892481190464274 21.500425404133928 2 11.555970584802147 25.72162283846654 42.06840200770398 20.654004569027332 3 18.67569325173834 30.82823364626236 30.94328925647396 19.552783845525337 4 12.289273148688533 21.27361558086408 39.35902352803855 27.07808774240883 5 11.417184972736823 39.06388087575662 36.277534141501725 13.241400010004837 6 27.13978422904404 42.14203531706991 17.054909873105334 13.663270580780711 7 20.524920378182788 37.60317486785279 23.251238098414234 18.620666655550185 8 20.70333994764136 41.17656867485952 22.522552567074086 15.597538810425037 9 22.270764202697972 21.77218989178103 23.554718113754983 32.40232779176602 10 13.983425322239082 32.84754297910657 33.34111487218823 19.827916826466126 11 28.064499991662363 26.431989861428406 26.687121679534425 18.81638846737481 12 20.538260159076888 29.729369195110973 33.11934101482383 16.61302963098831 13 26.01590768871621 26.055927031398507 28.23364626236014 19.694519017525135 14 17.858631671974788 27.348218305514333 30.428040219439396 24.365109803071487 15 19.622817695219354 36.51598272498374 25.93420153073986 17.926998049057044 16 16.481848955293568 34.334406109823405 30.40904467308109 18.77470026180193 17 16.55661163915291 34.69234617308654 31.900950475237615 16.85009171252293 18 17.030749733191037 29.892609391675563 37.378268409818574 15.698372465314833 19 18.200463557386072 31.096696736756098 32.88922979440063 17.813609911457203 20 18.140434543362623 31.031665304897366 33.90805556018742 16.919844591552582 21 15.250704507178469 32.842540561271285 32.47903153190709 19.42772339964316 22 16.47129445898851 36.39092227910157 31.723666438778743 15.41411682313118 23 14.700438545296892 33.74464324423471 34.38828767237498 17.16663053809341 24 17.808607493621917 33.06598189124744 34.6534158176452 14.471994797485452 25 15.855997065248204 33.943072485034435 34.34993580230445 15.850994647412916 26 15.280719014190192 33.83135182004636 35.46047256173817 15.427456604025277 27 15.835987393907056 34.21320304813993 34.18152106851645 15.769288489436562 28 14.958896800120058 32.8992346300712 33.821346984375786 18.320521585432957 29 17.12160877757583 32.67912824531857 33.969752046822634 16.22951093028297 30 16.385132318364487 32.725241374710265 33.63404425619903 17.255582050726208 31 17.46177319037535 33.24773640592954 32.739157259342015 16.5513331443531 32 16.54132830868253 34.05145820479898 33.65293225058779 15.754281235930701 33 15.06061262943756 35.208684197362054 34.96690066865652 14.763802504543863 34 15.394107151790031 33.62625268879958 34.94355605209184 16.036084107318537 35 15.195677910990312 34.158176451951775 34.15317403411649 16.492971602941424 36 14.688766237014558 34.25322239082223 35.425455636891165 15.632555735272046 37 15.512497707225158 34.40996481632789 32.55740274465992 17.520134731787028 38 15.685914858848443 35.106968368044555 33.3511197078588 15.855997065248204 39 17.34171516232846 32.742492204565536 33.1560254122826 16.7597672208234 40 17.239999333010957 32.57074252555402 35.09529605976222 15.09396208167281 41 16.85481315969385 32.5890847242834 33.93473512197562 16.621366994047122 42 16.699738206799953 34.17318370545764 33.77966016908172 15.347417918660685 43 14.983908889296494 34.35327074752797 35.17199979990329 15.490820563272248 44 14.293575228026882 34.4449817411749 33.79633489519935 17.46510813559887 45 15.150656150472727 33.53454169515266 34.52168548131597 16.793116673058645 46 16.241183238565306 32.90923946574178 33.20604959063547 17.643527705057444 47 16.871487885811476 32.68579813576562 32.255590201930936 18.18712377649197 48 15.175668239649164 33.11600606960031 32.80585616381251 18.90246952693802 49 16.568007870470726 32.79084891030665 32.88089243134181 17.76025078788081 50 15.475813309766387 34.845175167997866 32.68413066315386 16.99488085908189 51 14.560370845908857 33.77966016908172 33.621250270964296 18.03871871404512 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1033.0 1 592.5 2 152.0 3 232.5 4 313.0 5 330.0 6 347.0 7 418.0 8 489.0 9 410.0 10 331.0 11 554.0 12 777.0 13 1301.5 14 1826.0 15 1904.5 16 1983.0 17 1451.0 18 919.0 19 733.0 20 547.0 21 855.5 22 1164.0 23 812.5 24 461.0 25 539.5 26 1076.0 27 1534.0 28 1847.0 29 2160.0 30 3302.0 31 4444.0 32 5536.5 33 6629.0 34 6927.5 35 7226.0 36 6570.5 37 5915.0 38 4888.5 39 3862.0 40 3254.5 41 2647.0 42 2317.5 43 1988.0 44 1890.0 45 1792.0 46 1736.0 47 1680.0 48 1621.0 49 1562.0 50 1523.0 51 1484.0 52 1477.5 53 1471.0 54 1579.0 55 1687.0 56 1268.5 57 850.0 58 752.5 59 655.0 60 574.5 61 494.0 62 435.5 63 377.0 64 298.5 65 220.0 66 182.5 67 145.0 68 117.0 69 89.0 70 71.5 71 54.0 72 39.5 73 25.0 74 19.5 75 9.0 76 4.0 77 2.5 78 1.0 79 1.5 80 2.0 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04668923312934585 2 0.0033349452235247037 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0033349452235247037 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0033349452235247037 17 0.0016674726117623519 18 0.005002417835287055 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0033349452235247037 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 59971.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.96096209069167 #Duplication Level Percentage of deduplicated Percentage of total 1 88.14303856889497 59.90285702739045 2 7.191539279003218 9.774878566281156 3 2.155870227826624 4.395450444773279 4 0.9270609814367057 2.5201582486071747 5 0.4809402316705399 1.6342580426225015 6 0.2713714489472367 1.1065598852639533 7 0.15231797921223372 0.7246173487681364 8 0.10497503713654541 0.5707363615444571 9 0.07573279967223866 0.46321865347922614 >10 0.4015930841041051 5.094294853423225 >50 0.0441051014371546 2.2530562447189864 >100 0.039204008129077664 5.025615303524574 >500 0.009801002023542608 4.629294412460762 >1k 0.002450250505885652 1.9050046071421265 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1144 1.9075886678561305 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 986 1.6441279951976788 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 682 1.137216321221924 No Hit CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 595 0.9921462039985993 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 517 0.8620833402811359 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 470 0.7837121275283054 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 267 0.44521518734054794 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 251 0.4185356255523503 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 237 0.3951910089876774 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 231 0.3851861733171033 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 215 0.35850661152890567 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 189 0.3151523236230845 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 177 0.29514265228193626 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 160 0.26679561788197625 No Hit GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 153 0.2551233095996398 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 127 0.21176902169381867 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 115 0.19175935035267044 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 109 0.18175451468209633 No Hit TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT 108 0.180087042070334 Illumina Paired End PCR Primer 2 (95% over 21bp) GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 106 0.1767520968468093 No Hit GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 103 0.17174967901152224 No Hit TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 100 0.16674726117623517 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92 0.15340748028213638 No Hit GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA 92 0.15340748028213638 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA 89 0.1484050624468493 No Hit GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 88 0.14673758983508695 No Hit ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.14006769938803756 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 83 0.1384002267762752 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 82 0.13673275416451286 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAA 81 0.1350652815527505 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 77 0.12839539110570108 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 76 0.12672791849393875 No Hit ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA 68 0.11338813759983993 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT 65 0.10838571976455287 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 62 0.1033833019292658 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0033349452235247037 15 0.0 0.0 0.0033349452235247037 16 0.0 0.0 0.0033349452235247037 17 0.0 0.0 0.0033349452235247037 18 0.0 0.0 0.0033349452235247037 19 0.0 0.0 0.005002417835287055 20 0.0 0.0 0.005002417835287055 21 0.0 0.0 0.005002417835287055 22 0.0 0.0 0.00833736305881176 23 0.0 0.0 0.01667472611762352 24 0.0 0.0 0.025012089176435277 25 0.0 0.0 0.02667956178819763 26 0.0 0.0 0.03501692484700939 27 0.0 0.0 0.0450217605175835 28 0.0 0.0 0.06336395924696937 29 0.0 0.0 0.0967134114822164 30 0.0 0.0 0.14340264461156227 31 0.0 0.0 0.2601257274349269 32 0.0 0.0 0.4101982624935385 33 0.0 0.0 0.5569358523286255 34 0.0 0.0 0.7436927848460089 35 0.0 0.0 0.9354521351986793 36 0.0 0.0 1.1889079721865567 37 0.0 0.0 1.5290723849860766 38 0.0 0.0 1.877574160844408 39 0.0 0.0 2.3111170399026197 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGCGA 85 0.0 39.705883 27 GTATGCC 85 0.0 39.705883 42 ATCTCGT 85 0.0 39.705883 37 ATCTCCG 85 0.0 39.705883 10 GCCCACG 85 0.0 39.705883 18 TGCCGTC 85 0.0 39.705883 45 CCACGAG 85 0.0 39.705883 20 TATGCCG 85 0.0 39.705883 43 CATCTCC 85 0.0 39.705883 9 TGCGACC 85 0.0 39.705883 29 CCCACGA 85 0.0 39.705883 19 TCTCGTA 85 0.0 39.705883 38 CTGCGAC 85 0.0 39.705883 28 CGTATGC 85 0.0 39.705883 41 GACTGCG 85 0.0 39.705883 26 CGAGCCC 85 0.0 39.705883 15 CCGAGCC 85 0.0 39.705883 14 TCGTATG 85 0.0 39.705883 40 CGAGACT 85 0.0 39.705883 23 TCCGAGC 85 0.0 39.705883 13 >>END_MODULE