H23NVBCXX l02n01 48h_33.34100000006a2d.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 48h_33.34100000006a2d.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	46688
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	544	1.1651816312542838	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	465	0.995973269362577	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	336	0.7196710075394105	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	307	0.6575565455791638	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	247	0.5290438656614119	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	227	0.48620630568882794	No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	137	0.29343728581220013	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	117	0.2505997258396162	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	106	0.22703906785469502	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	104	0.22275531185743663	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	104	0.22275531185743663	No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	95	0.20347840986977384	No Hit
GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	93	0.19919465387251545	No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	83	0.17777587388622343	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	78	0.16706648389307746	No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	63	0.13493831391363947	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59	0.12637080191912267	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA	58	0.12422892392049349	No Hit
GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51	0.1092357779300891	No Hit
TTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTCT	50	0.1070938999314599	Illumina Paired End PCR Primer 2 (95% over 21bp)
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	48	0.1028101439342015	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCGA	35	1.1787597E-7	45.0	27
ATCTCGT	40	6.5756467E-9	45.0	37
CCTATCT	35	1.1787597E-7	45.0	34
ATCTCCG	40	6.5756467E-9	45.0	10
GCCCACG	40	6.5756467E-9	45.0	18
GCGACCT	35	1.1787597E-7	45.0	30
TATGCCG	40	6.5756467E-9	45.0	43
TGCGACC	35	1.1787597E-7	45.0	29
TCTCGTA	40	6.5756467E-9	45.0	38
CTGCGAC	35	1.1787597E-7	45.0	28
ACGAGAC	40	6.5756467E-9	45.0	22
CGTATGC	40	6.5756467E-9	45.0	41
GACTGCG	35	1.1787597E-7	45.0	26
CGAGCCC	40	6.5756467E-9	45.0	15
TCGTATG	40	6.5756467E-9	45.0	40
TCTCCGA	40	6.5756467E-9	45.0	11
ACCTATC	35	1.1787597E-7	45.0	33
CGAGACT	35	1.1787597E-7	45.0	23
GACCTAT	35	1.1787597E-7	45.0	32
TCCGAGC	40	6.5756467E-9	45.0	13

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content