##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_31.34100000006a03.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15339 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.074972292848294 38.0 38.0 38.0 32.0 38.0 2 36.399830497424865 38.0 38.0 38.0 32.0 38.0 3 36.175239585370626 38.0 38.0 38.0 32.0 38.0 4 36.38705261099159 38.0 38.0 38.0 32.0 38.0 5 36.50335745485364 38.0 38.0 38.0 32.0 38.0 6 38.56111871699589 40.0 38.0 40.0 38.0 40.0 7 38.14935784601343 40.0 38.0 40.0 38.0 40.0 8 38.11376230523502 40.0 38.0 40.0 38.0 40.0 9 38.42747245583154 40.0 38.0 40.0 38.0 40.0 10 38.2301323423952 40.0 38.0 40.0 38.0 40.0 11 38.322185279353285 40.0 38.0 40.0 38.0 40.0 12 37.70747767129539 40.0 38.0 40.0 32.0 40.0 13 38.198122433013886 40.0 38.0 40.0 38.0 40.0 14 37.88728078753504 40.0 38.0 40.0 32.0 40.0 15 38.018580089966754 40.0 38.0 40.0 32.0 40.0 16 37.379229415216116 40.0 38.0 40.0 32.0 40.0 17 37.40432883499576 40.0 38.0 40.0 32.0 40.0 18 37.11454462481257 40.0 38.0 40.0 32.0 40.0 19 37.54012647499837 40.0 38.0 40.0 32.0 40.0 20 37.74874502901102 40.0 38.0 40.0 32.0 40.0 21 37.9270486993937 40.0 38.0 40.0 32.0 40.0 22 37.97959449768564 40.0 38.0 40.0 32.0 40.0 23 38.00332485820458 40.0 38.0 40.0 32.0 40.0 24 37.75780689745094 40.0 38.0 40.0 32.0 40.0 25 37.381837147141276 40.0 38.0 40.0 32.0 40.0 26 37.29910685181564 40.0 38.0 40.0 32.0 40.0 27 37.49012321533346 40.0 38.0 40.0 32.0 40.0 28 37.51300606297673 40.0 38.0 40.0 32.0 40.0 29 37.72762240041724 40.0 38.0 40.0 32.0 40.0 30 37.76947649781602 38.0 38.0 40.0 32.0 40.0 31 37.92444096746854 40.0 38.0 40.0 32.0 40.0 32 37.57943803377013 40.0 38.0 40.0 32.0 40.0 33 37.59462807223417 40.0 38.0 40.0 32.0 40.0 34 37.70741247799726 40.0 38.0 40.0 32.0 40.0 35 37.58067670643458 40.0 38.0 40.0 32.0 40.0 36 37.25464502249169 40.0 38.0 40.0 32.0 40.0 37 37.30497424864724 40.0 38.0 40.0 32.0 40.0 38 37.45198513592803 40.0 38.0 40.0 32.0 40.0 39 37.21781080904883 40.0 38.0 40.0 32.0 40.0 40 37.25216767716279 40.0 38.0 40.0 32.0 40.0 41 37.36312667057827 40.0 38.0 40.0 32.0 40.0 42 37.3551078949084 40.0 38.0 40.0 32.0 40.0 43 37.726840080839686 40.0 38.0 40.0 32.0 40.0 44 37.35399960884021 40.0 38.0 40.0 32.0 40.0 45 37.55238281504661 40.0 38.0 40.0 32.0 40.0 46 36.981093943542604 38.0 38.0 40.0 32.0 40.0 47 36.97907295130061 38.0 38.0 40.0 32.0 40.0 48 36.98070278375383 40.0 38.0 40.0 32.0 40.0 49 36.68511637003716 38.0 38.0 40.0 32.0 40.0 50 36.538431449247014 38.0 38.0 40.0 27.0 40.0 51 35.96583871178043 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 1.2563150816071982 2201 2 0.7641119524849032 2201 3 -0.540939007902324 2201 4 0.8694474557246394 2201 5 0.8644396121943672 2201 6 -0.017682038422528024 2201 7 0.8712121651999354 2201 8 1.2171671714708552 2201 9 0.18012755586802598 2201 10 0.587172337525594 2201 11 0.8475405215758443 2201 12 1.3880972262188251 2201 13 0.23779045167290747 2201 14 -0.20200655549160018 2201 15 0.7228911162810618 2201 16 1.53920945612861 2201 17 1.6531995071906778 2201 18 0.47000352040773663 2201 19 0.9743610943203365 2201 20 0.09945828037198368 2201 21 0.9020022542373098 2201 22 0.9680977358490921 2201 23 0.8336845671186737 2201 24 0.1320275120410983 2201 25 1.4091868534293823 2201 26 1.3842995669286466 2201 27 1.3595080679183837 2201 28 0.4360501016445255 2201 29 0.3309788166241958 2201 30 0.792127549005933 2201 31 0.9685426193170841 2201 32 0.4086530198113181 2201 33 -1.6350671108279755 2201 34 0.002492367988295996 2201 35 0.009983042068633097 2201 36 0.21191237845790312 2201 37 1.3010449250327127 2201 38 0.8445797263324408 2201 39 1.1818822315969086 2201 40 0.5564272609687109 2201 41 0.9648027187938766 2201 42 0.10512844228538398 2201 43 -1.4849503720665496 2201 44 -1.184253860320453 2201 45 -2.4787742072490815 2201 46 -0.1733942172460985 2201 47 0.9218060977367983 2201 48 0.4756504508474677 2201 49 0.8762833772005081 2201 50 0.7091261367959802 2201 51 -0.02226511599814529 1101 1 -0.7436849183928018 1101 2 -2.50861532024237 1101 3 -0.7682117351750506 1101 4 -2.9941889079117203 1101 5 -2.0446512968965393 1101 6 -1.1085911293316215 1101 7 -0.44696965298188474 1101 8 -1.0101055558018714 1101 9 -1.2289633532228805 1101 10 0.1326268829801407 1101 11 -2.6979140238787025 1101 12 -2.248266410144815 1101 13 0.055972269854727585 1101 14 -1.0201883736734203 1101 15 -1.140745247355298 1101 16 -1.5516996347804834 1101 17 -0.16498231099114236 1101 18 -0.4845419341377166 1101 19 -0.9801843602251168 1101 20 -1.127814446900743 1101 21 -1.143452291217237 1101 22 -0.3046295368781813 1101 23 -2.57540634197224 1101 24 -2.1861543061407147 1101 25 -0.09081314657061768 1101 26 -2.206609523980447 1101 27 -1.8677646593543429 1101 28 -1.4275862619918342 1101 29 -2.578112092466718 1101 30 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0.046825793092757806 1102 1 0.5527577693542369 1102 2 -1.1015006957364406 1102 3 0.21993055731506672 1102 4 -1.1384576826152824 1102 5 0.10554633156196047 1102 6 0.4783653924075111 1102 7 -0.002305621361330168 1102 8 -0.09508579295602004 1102 9 0.6307204412435254 1102 10 0.373733602347734 1102 11 0.7092006006272271 1102 12 0.47110117878798974 1102 13 -0.3056878091966553 1102 14 0.16360609272974358 1102 15 0.9126144364391706 1102 16 0.4206323810297974 1102 17 0.4358082028428498 1102 18 0.5371971962970647 1102 19 -0.6995519491579216 1102 20 0.19629622503601496 1102 21 0.9573382226167553 1102 22 1.363354652845139 1102 23 1.4068070967629396 1102 24 1.179458342080629 1102 25 1.6305307269471712 1102 26 1.3941809898535453 1102 27 0.6678084631753052 1102 28 -0.9177048390669356 1102 29 1.0246547059522584 1102 30 0.19528960434190168 1102 31 -0.5492439419477364 1102 32 1.3197202134872086 1102 33 1.7107826915435638 1102 34 0.05387576719778053 1102 35 1.1977300776417579 1102 36 0.4075645523709497 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34 589.0 35 732.0 36 975.0 37 1421.0 38 2537.0 39 6982.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 7.8774046299315295 11.626997065536354 9.598956635148353 70.89664166938377 2 2.699002542538627 12.83004107177782 76.85637916422192 7.614577221461634 3 7.23645609231371 13.051698285416261 73.65538822609035 6.056457396179673 4 6.323749918508377 72.3645609231371 14.309928939305038 7.001760219049482 5 3.6312667057826458 16.578655714192582 75.92411500097789 3.865962579046874 6 11.767390312275898 77.52787013495012 6.369385227198644 4.335354325575331 7 4.133255101375578 13.364626116435229 13.019101636351781 69.48301714583741 8 6.917008931481844 77.10411369711194 7.125627485494491 8.853249885911728 9 6.004302757676511 10.430927700632376 5.971706108612035 77.59306343307908 10 3.833365929982398 72.80135602060108 14.512028163504793 8.853249885911728 11 8.885846534976205 10.196231827368146 68.27694113045179 12.640980507203858 12 5.886954821044397 9.772475389529957 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8.05789164873851 10.86120346828346 26 69.00306448457978 12.47310425767751 11.553758883745191 6.970072373997523 27 9.407392920007823 72.89914596779451 11.591368407327726 6.102092704869939 28 6.0760153856183585 9.583414824955995 11.063302692483212 73.27726709694244 29 5.058999934806701 9.981093943542604 14.179542343047135 70.78036377860356 30 5.417916286347634 7.047855000651976 75.17277350371626 12.36145520928413 31 7.295130060629767 7.6536932003390055 76.24356216180976 8.807614577221463 32 65.97561770649976 7.9144663928548145 16.839428906708388 9.270486993937023 33 7.275572071191082 10.307060434187365 14.948823260968771 67.46854423365278 34 7.673251189777691 69.74379033835322 11.786948301714585 10.796010170154508 35 65.59097724753894 12.601864528326487 11.689158354521155 10.117999869613403 36 7.581980572397158 72.11030706043418 13.345068126996546 6.96264424017211 37 5.352369776386987 74.11826064280592 13.781863224460524 6.747506356346568 38 3.8724819088597693 11.910815568159592 73.81837147141273 10.398331051567899 39 3.2401069170089314 10.241867136058413 18.149814199100334 68.36821174783232 40 3.1488362996283983 7.927505052480605 74.62024903839885 14.303409609492142 41 4.4527022622074455 8.299106851815633 14.916226611904296 72.33196427407262 42 65.59749657735185 8.429493448073538 15.50296629506487 10.470043679509747 43 5.221983180129083 11.428385162005346 71.75174392072495 11.597887737140622 44 4.765630093226416 71.88213051698285 16.4156724688702 6.93656692092053 45 4.459221592020341 12.595345198513593 73.76621683290958 9.17921637655649 46 65.50622595997132 10.606949605580546 11.923854227785384 11.962970206662755 47 6.630158419714453 9.433470239259405 11.252363257057175 72.68400808396896 48 6.473694504204968 7.63413521090032 7.190820783623443 78.70134950127127 49 67.4489862442141 8.670708651150662 9.205293695808072 14.675011408827174 50 6.708390377469196 7.516787274268205 71.20412021644175 14.570702131820848 51 6.0438127526405 8.338766462381015 11.17485982527057 74.44256095970792 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 33.0 1 16.5 2 0.0 3 2.0 4 4.0 5 3.5 6 3.0 7 4.0 8 5.0 9 6.0 10 7.0 11 9.0 12 11.0 13 14.0 14 17.0 15 27.0 16 37.0 17 25.5 18 14.0 19 12.5 20 11.0 21 11.0 22 11.0 23 11.5 24 12.0 25 13.5 26 22.0 27 29.0 28 25.5 29 22.0 30 39.5 31 57.0 32 85.0 33 113.0 34 228.5 35 344.0 36 397.5 37 451.0 38 619.0 39 787.0 40 840.0 41 893.0 42 782.0 43 671.0 44 526.5 45 382.0 46 315.5 47 249.0 48 220.0 49 191.0 50 623.0 51 1055.0 52 5396.0 53 9737.0 54 4932.5 55 128.0 56 71.0 57 14.0 58 12.5 59 11.0 60 10.5 61 10.0 62 7.0 63 4.0 64 3.5 65 3.0 66 2.5 67 2.0 68 2.5 69 3.0 70 2.0 71 1.0 72 0.5 73 0.0 74 0.5 75 0.5 76 0.0 77 0.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026077319251580938 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.013038659625790469 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.013038659625790469 27 0.0 28 0.0 29 0.0 30 0.0065193298128952346 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0065193298128952346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 15339.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.82384770845557 #Duplication Level Percentage of deduplicated Percentage of total 1 84.05267008046819 14.981419910033248 2 6.803218727139722 2.425190690397027 3 2.7066569129480618 1.447291218462742 4 1.2435991221653255 0.8866288545537517 5 0.9144111192392099 0.8149162266119043 6 0.6583760058522311 0.7040876197926853 7 0.36576444769568395 0.45635308690266635 8 0.25603511338697876 0.36508246952213314 9 0.4023408924652524 0.6454136514766282 >10 2.1580102414045355 6.858334963165786 >50 0.2926115581565472 3.918117217550036 >100 0.10972933430870518 6.408501206076016 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0365764447695684 60.08866288545538 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC 9217 60.08866288545538 No Hit TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT 425 2.7707151704804747 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG 336 2.190494817132799 No Hit TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT 222 1.447291218462742 RNA PCR Primer, Index 40 (95% over 21bp) CAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAG 99 0.6454136514766282 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC 87 0.5671816937218854 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC 85 0.554143034096095 No Hit CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 75 0.4889497359671426 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGAG 70 0.45635308690266635 No Hit AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC 64 0.417237108025295 No Hit GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC 62 0.4041984483995046 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGA 59 0.38464045896081883 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCGA 44 0.2868505117673903 No Hit TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC 35 0.22817654345133317 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACATC 35 0.22817654345133317 No Hit TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 34 0.22165721363843793 No Hit TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 31 0.2020992241997523 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 29 0.18906056457396178 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACATC 29 0.18906056457396178 No Hit TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC 28 0.18254123476106657 No Hit AAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCCCA 28 0.18254123476106657 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCACTGTCTCTTATACACATCTC 27 0.17602190494817133 No Hit GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC 26 0.1695025751352761 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCACTGTCTCTTATACACATC 24 0.15646391550948563 No Hit GTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCA 24 0.15646391550948563 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACA 24 0.15646391550948563 No Hit GTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAG 23 0.1499445856965904 No Hit TGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTC 22 0.14342525588369515 No Hit TGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATC 22 0.14342525588369515 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACA 20 0.1303865962579047 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 20 0.1303865962579047 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGA 19 0.12386726644500945 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19 0.12386726644500945 No Hit CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC 19 0.12386726644500945 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTCCGA 18 0.11734793663211421 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 17 0.11082860681921897 No Hit AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA 17 0.11082860681921897 No Hit AAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAG 16 0.10430927700632375 No Hit AAAAAGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACATCTCCGA 16 0.10430927700632375 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCC 16 0.10430927700632375 No Hit TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 16 0.10430927700632375 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0065193298128952346 2 0.0 0.0 0.013038659625790469 3 0.0 0.0 0.013038659625790469 4 0.0 0.0 0.013038659625790469 5 0.0 0.0 0.013038659625790469 6 0.0 0.0 0.013038659625790469 7 0.0 0.0 0.013038659625790469 8 0.0 0.0 0.013038659625790469 9 0.0 0.0 0.013038659625790469 10 0.0 0.0 0.013038659625790469 11 0.0 0.0 0.013038659625790469 12 0.0 0.0 0.019557989438685704 13 0.0 0.0 0.019557989438685704 14 0.0 0.0 0.026077319251580938 15 0.0 0.0 0.04563530869026664 16 0.0 0.0 0.08475128756763804 17 0.0 0.0 0.11082860681921898 18 0.0 0.0 0.23469587326422844 19 0.0 0.0 0.29336984158028556 20 0.0 0.0 0.33248582045765696 21 0.0 0.0 0.5019883955929331 22 0.0 0.0 0.6584523111024186 23 0.0 0.0 0.9518221526827042 24 0.0 0.0 1.5711584849077516 25 0.0 0.0 1.7145837407914466 26 0.0 0.0 2.705521872351522 27 0.0 0.0 3.024969033183389 28 0.0 0.0 4.563530869026664 29 0.0 0.0 6.036899406740987 30 0.0 0.0 6.708390377469196 31 0.0 0.0 8.553360714518547 32 0.0 0.0 9.818110698220224 33 0.0 0.0 10.69822022296108 34 0.0 0.0 13.162526892235478 35 0.0 0.0 14.18606167286003 36 0.0 0.0 15.763739487580677 37 0.0 0.0 16.656887671947324 38 0.0 0.0 17.439207249494753 39 0.0 0.0 18.0585435817198 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTCT 50 1.8189894E-11 44.973854 45 TGCCGTC 1035 0.0 42.801205 45 ATGCCGT 1080 0.0 41.017822 44 AGAATCT 1075 0.0 40.79024 34 GAATCTC 1075 0.0 40.79024 35 CAGAATC 1075 0.0 40.79024 33 TTGCAGA 1090 0.0 40.77476 30 CTTGCAG 1085 0.0 40.75473 29 TGCAGAA 1090 0.0 40.641514 31 GCAGAAT 1085 0.0 40.621548 32 AATCTCG 1080 0.0 40.6014 36 CCTTGCA 1090 0.0 40.567783 28 ACCTTGC 1090 0.0 40.567783 27 AGACCTT 1090 0.0 40.567783 25 GACCTTG 1090 0.0 40.567783 26 GAGACCT 1090 0.0 40.567783 24 ATCTCGT 1100 0.0 40.47647 37 TCTCGTA 1100 0.0 40.47647 38 CGTATGC 1110 0.0 40.314404 41 TCGTATG 1110 0.0 40.314404 40 >>END_MODULE