H23NVBCXX l02n01 48h_31.34100000006a03.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 48h_31.34100000006a03.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15339
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC	9217	60.08866288545538	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT	425	2.7707151704804747	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG	336	2.190494817132799	No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT	222	1.447291218462742	RNA PCR Primer, Index 40 (95% over 21bp)
CAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAG	99	0.6454136514766282	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC	87	0.5671816937218854	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC	85	0.554143034096095	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	75	0.4889497359671426	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGAG	70	0.45635308690266635	No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC	64	0.417237108025295	No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC	62	0.4041984483995046	No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGA	59	0.38464045896081883	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCGA	44	0.2868505117673903	No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC	35	0.22817654345133317	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACATC	35	0.22817654345133317	No Hit
TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA	34	0.22165721363843793	No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA	31	0.2020992241997523	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	29	0.18906056457396178	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACATC	29	0.18906056457396178	No Hit
TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC	28	0.18254123476106657	No Hit
AAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCCCA	28	0.18254123476106657	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCACTGTCTCTTATACACATCTC	27	0.17602190494817133	No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC	26	0.1695025751352761	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCACTGTCTCTTATACACATC	24	0.15646391550948563	No Hit
GTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCA	24	0.15646391550948563	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACA	24	0.15646391550948563	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAG	23	0.1499445856965904	No Hit
TGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTC	22	0.14342525588369515	No Hit
TGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATC	22	0.14342525588369515	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACA	20	0.1303865962579047	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	20	0.1303865962579047	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGA	19	0.12386726644500945	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.12386726644500945	No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC	19	0.12386726644500945	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTCCGA	18	0.11734793663211421	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	17	0.11082860681921897	No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA	17	0.11082860681921897	No Hit
AAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAG	16	0.10430927700632375	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACATCTCCGA	16	0.10430927700632375	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCC	16	0.10430927700632375	No Hit
TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA	16	0.10430927700632375	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGTCT	50	1.8189894E-11	44.973854	45
TGCCGTC	1035	0.0	42.801205	45
ATGCCGT	1080	0.0	41.017822	44
AGAATCT	1075	0.0	40.79024	34
GAATCTC	1075	0.0	40.79024	35
CAGAATC	1075	0.0	40.79024	33
TTGCAGA	1090	0.0	40.77476	30
CTTGCAG	1085	0.0	40.75473	29
TGCAGAA	1090	0.0	40.641514	31
GCAGAAT	1085	0.0	40.621548	32
AATCTCG	1080	0.0	40.6014	36
CCTTGCA	1090	0.0	40.567783	28
ACCTTGC	1090	0.0	40.567783	27
AGACCTT	1090	0.0	40.567783	25
GACCTTG	1090	0.0	40.567783	26
GAGACCT	1090	0.0	40.567783	24
ATCTCGT	1100	0.0	40.47647	37
TCTCGTA	1100	0.0	40.47647	38
CGTATGC	1110	0.0	40.314404	41
TCGTATG	1110	0.0	40.314404	40

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content