##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_30.341000000069f5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23331 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.14114268569714 38.0 38.0 38.0 32.0 38.0 2 36.20753503921821 38.0 38.0 38.0 32.0 38.0 3 36.10878230680211 38.0 38.0 38.0 32.0 38.0 4 36.29407226436929 38.0 38.0 38.0 32.0 38.0 5 36.249753546783246 38.0 38.0 38.0 32.0 38.0 6 38.1972054348292 40.0 38.0 40.0 38.0 40.0 7 37.98028374265998 40.0 38.0 40.0 32.0 40.0 8 37.92550683639792 40.0 38.0 40.0 32.0 40.0 9 38.006772105782005 40.0 38.0 40.0 32.0 40.0 10 37.984012686982986 40.0 38.0 40.0 32.0 40.0 11 37.933307616475936 40.0 38.0 40.0 32.0 40.0 12 37.773091594873776 40.0 38.0 40.0 32.0 40.0 13 37.90840512622691 40.0 38.0 40.0 32.0 40.0 14 37.761261840469764 40.0 38.0 40.0 32.0 40.0 15 37.807037846641805 40.0 38.0 40.0 32.0 40.0 16 37.66439501092967 40.0 38.0 40.0 32.0 40.0 17 37.57498607003558 40.0 38.0 40.0 32.0 40.0 18 37.55548411984056 40.0 38.0 40.0 32.0 40.0 19 37.728429985855726 40.0 38.0 40.0 32.0 40.0 20 37.73250182161073 40.0 38.0 40.0 32.0 40.0 21 37.814538596716815 40.0 38.0 40.0 32.0 40.0 22 37.832797565470834 40.0 38.0 40.0 32.0 40.0 23 37.79160773220179 40.0 38.0 40.0 32.0 40.0 24 37.740816938836744 40.0 38.0 40.0 32.0 40.0 25 37.667481033817666 40.0 38.0 40.0 32.0 40.0 26 37.58565856585658 40.0 38.0 40.0 32.0 40.0 27 37.58930178732159 40.0 38.0 40.0 32.0 40.0 28 37.556212764133555 40.0 38.0 40.0 32.0 40.0 29 37.65363679225065 40.0 38.0 40.0 32.0 40.0 30 37.636577943508634 40.0 38.0 40.0 32.0 40.0 31 37.65517980369466 40.0 38.0 40.0 32.0 40.0 32 37.538510993956535 40.0 38.0 40.0 32.0 40.0 33 37.52981012386953 40.0 38.0 40.0 32.0 40.0 34 37.6028888603146 40.0 38.0 40.0 32.0 40.0 35 37.58004371865758 40.0 38.0 40.0 32.0 40.0 36 37.46183189747546 40.0 38.0 40.0 32.0 40.0 37 37.501564442158504 40.0 38.0 40.0 32.0 40.0 38 37.54258282971154 40.0 38.0 40.0 32.0 40.0 39 37.42934293429343 40.0 38.0 40.0 32.0 40.0 40 37.39278213535639 40.0 38.0 40.0 32.0 40.0 41 37.47670481333848 40.0 38.0 40.0 32.0 40.0 42 37.428228537139425 40.0 38.0 40.0 32.0 40.0 43 37.577814924349575 40.0 38.0 40.0 32.0 40.0 44 37.43020016287343 40.0 38.0 40.0 32.0 40.0 45 37.55852728129956 40.0 38.0 40.0 32.0 40.0 46 37.305944880202304 40.0 38.0 40.0 32.0 40.0 47 37.373965968025374 40.0 38.0 40.0 32.0 40.0 48 37.33453345334534 40.0 38.0 40.0 32.0 40.0 49 37.236209335219236 40.0 38.0 40.0 32.0 40.0 50 37.185247096138184 38.0 38.0 40.0 32.0 40.0 51 36.67699627105568 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.5591700702701488 2201 2 -0.10022964526573475 2201 3 -1.424685717109476 2201 4 -1.0436554207654822 2201 5 1.153076819920102 2201 6 0.5009381399376878 2201 7 -1.2844519249956008 2201 8 -1.0797299488537604 2201 9 -0.9533251851453102 2201 10 -1.3827719341578018 2201 11 -0.4056345776676693 2201 12 -0.34158217417853365 2201 13 -0.6022845986048821 2201 14 -0.5561662573503909 2201 15 0.144594214067439 2201 16 -0.5229516818249635 2201 17 -0.15502396990199685 2201 18 -2.1029718318279436 2201 19 -1.8483772214200158 2201 20 -1.0910764575355003 2201 21 -0.4055236884756681 2201 22 -0.0761528648770664 2201 23 -1.4105674169801716 2201 24 0.11166957904445951 2201 25 -0.9009588779251487 2201 26 -1.3325574107715994 2201 27 -0.3338877344555229 2201 28 0.8409659233501827 2201 29 0.545230389391854 2201 30 -0.958653681715397 2201 31 -0.39444488967248503 2201 32 0.6684405532945235 2201 33 0.457182291202443 2201 34 -0.5756979161451454 2201 35 -0.8409907935442149 2201 36 -1.3467390528928505 2201 37 0.04255423432742589 2201 38 -0.4924321711343609 2201 39 0.07604615990591412 2201 40 -1.478278741299647 2201 41 -0.34761117854273493 2201 42 0.05121450675403594 2201 43 -1.8610369701763787 2201 44 -0.8628770136222172 2201 45 -0.15002241190138932 2201 46 -1.3230344656701618 2201 47 -2.4245686189230966 2201 48 -0.3798001936532387 2201 49 0.09245954921571098 2201 50 -2.9129727710593016 2201 51 1.028636590463293 1101 1 0.5497314448813668 1101 2 0.9272324759463899 1101 3 0.6463370101632506 1101 4 1.285890033779971 1101 5 1.1758040926473754 1101 6 0.9706351096346566 1101 7 1.1824041356104615 1101 8 1.2318230814492708 1101 9 0.7436445118243853 1101 10 1.4837053385694716 1101 11 0.2477745132414242 1101 12 0.835501159154802 1101 13 1.696010855940571 1101 14 1.712773136589 1101 15 1.3311472443704702 1101 16 1.479889227265943 1101 17 1.045733605855581 1101 18 0.9784675621114474 1101 19 1.2520015664587731 1101 20 0.25646142125237503 1101 21 1.2175823721303942 1101 22 0.5081274381532381 1101 23 1.4009856133228595 1101 24 1.7575029123777952 1101 25 1.2647608190445467 1101 26 0.8473668316526428 1101 27 1.1803168109990239 1101 28 0.9886931960774561 1101 29 1.6664425106039786 1101 30 1.8121796516179387 1101 31 0.8451384436608507 1101 32 2.0065087351127033 1101 33 1.5850232002933495 1101 34 1.578658144460917 1101 35 0.8114713276679097 1101 36 1.6276927652889697 1101 37 1.1808118100850038 1101 38 1.7547269197747326 1101 39 1.5788870689968206 1101 40 0.8010773193064153 1101 41 1.7451918517602962 1101 42 1.950835718875247 1101 43 2.1247584843690746 1101 44 1.2441305621353607 1101 45 0.9617200123410328 1101 46 0.6864352312995337 1101 47 1.796075320470841 1101 48 1.234783139680097 1101 49 0.23923985224601552 1101 50 0.3588075319710029 1101 51 1.2521214389481443 2202 1 -1.5487534036034845 2202 2 0.22268702140092955 2202 3 0.14128650511274543 2202 4 -0.30754430965436796 2202 5 0.4308545976978806 2202 6 -0.7768396378400908 2202 7 0.09402029722662064 2202 8 -0.49292439329820326 2202 9 -0.5366585184786459 2202 10 -0.37235526749113745 2202 11 -1.676467911001005 2202 12 -0.05338772973409078 2202 13 -0.008534598604882149 2202 14 -0.4589440351281695 2202 15 -0.9213780081547824 2202 16 -1.0403127929360778 2202 17 -0.585579525457554 2202 18 -2.137694054050165 2202 19 -1.7858772214200158 2202 20 -1.6501042353132789 2202 21 -0.31524591069788954 2202 22 0.5384304684562693 2202 23 -1.2126507503135073 2202 24 -1.0202748653999834 2202 25 -2.737764433480706 2202 26 -1.0061685218827137 2202 27 -1.67069329001108 2202 28 -2.2562562988720387 2202 29 -1.2603251661637032 2202 30 -0.6600425706042827 2202 31 -0.9326393341169279 2202 32 -2.647531668927698 2202 33 0.12037673564688589 2202 34 -1.3986145828118097 2202 35 -0.5597407935442149 2202 36 -1.0793779417817362 2202 37 -1.9782790990059098 2202 38 0.6360400510878605 2202 39 -0.35798161787186444 2202 40 -0.12063985241076125 2202 41 -1.0073334007649564 2202 42 -0.8723966043570783 2202 43 0.19799080760139987 2202 44 -0.16496034695555295 2202 45 -1.6291890785680536 2202 46 -2.010534465670162 2202 47 -1.2266519522564323 2202 48 -1.515216860319903 2202 49 -1.8415682285620676 2202 50 -1.916444993281523 2202 51 -1.0685856317589284 1102 1 -0.2797057845558655 1102 2 -0.7939796452657362 1102 3 0.2047785686048087 1102 4 -0.2543697064797712 1102 5 0.87450539134867 1102 6 0.5152238542234002 1102 7 -1.649630496424173 1102 8 -0.28419423456804793 1102 9 0.3038176719975496 1102 10 0.7252637801279107 1102 11 0.07115113661804173 1102 12 0.5878821115357553 1102 13 0.2986082585379748 1102 14 -0.25259482877896033 1102 15 -0.5562986430754151 1102 16 0.6565126038893254 1102 17 1.4985474586694352 1102 18 -0.8726146889707991 1102 19 -0.7073057928485866 1102 20 -0.27946931467835867 1102 21 0.27126202581004577 1102 22 0.23366856369436562 1102 23 -0.08646027412302715 1102 24 0.6241695790444624 1102 25 1.5392196935034193 1102 26 0.3192283035141159 1102 27 -0.1079948773126631 1102 28 -1.0840340766498144 1102 29 -0.2547696106081432 1102 30 0.021703461141747482 1102 31 0.05466225318465945 1102 32 -0.6610237324197641 1102 33 -0.8097819945118445 1102 34 -0.7962336304308621 1102 35 -0.8811693649727843 1102 36 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0.8040351350537449 2203 22 0.7639206645347016 2203 23 -0.7763762405095846 2203 24 -0.25230100919083753 2203 25 0.3324970044277933 2203 26 0.6637661186401687 2203 27 0.45839167730918007 2203 28 0.4512600409972407 2203 29 0.28052450703891196 2203 30 0.39061102416695803 2203 31 -0.04701841908425308 2203 32 -0.08707415258783158 2203 33 0.793579350025972 2203 34 -0.15841850438044247 2203 35 1.1020239123381401 2203 36 0.22127565298950458 2203 37 0.4874071755038969 2203 38 0.5755825347479941 2203 39 0.2874432187294431 2203 40 -0.524234623652589 2203 41 -0.5204052961897929 2203 42 0.032832153812861975 2203 43 -1.3297869701763787 2203 44 0.43307886873072476 2203 45 -0.49193417660727334 2203 46 0.5188772990357222 2203 47 -1.3786127365701546 2203 48 0.9308615710526453 2203 49 -0.269672803725463 2203 50 -0.12069335929459868 2203 51 1.462460119875061 1103 1 1.1522269885533802 1103 2 1.1001380017930913 1103 3 0.860240753478756 1103 4 1.1254622262933438 1103 5 0.844253290508334 1103 6 1.3538793164082747 1103 7 1.6512098397102832 1103 8 1.7309318158521236 1103 9 0.5760865795605739 1103 10 0.8874486540774953 1103 11 1.5686301282146857 1103 12 1.7374619434685243 1103 13 1.5944065778657048 1103 14 1.730598448531964 1103 15 0.300844214067439 1103 16 0.7766806711162104 1103 17 1.5287995595097712 1103 18 0.34923405052499845 1103 19 0.7288286609329262 1103 20 1.2600264836409707 1103 21 0.6569763115243319 1103 22 1.3227441939464626 1103 23 0.8706825830198284 1103 24 0.6888754613974015 1103 25 0.24426171031014832 1103 26 1.6637661186401687 1103 27 1.576038736132709 1103 28 2.1865541586442987 1103 29 0.809936271744796 1103 30 0.949434553578719 1103 31 1.276510992680457 1103 32 1.6482199650592264 1103 33 1.058285232378914 1103 34 1.0180520838548546 1103 35 0.9549650888087271 1103 36 1.3683344765189176 1103 37 1.5462307049156578 1103 38 1.1638178288656391 1103 39 0.8756785128470881 1103 40 0.711059493994469 1103 41 1.009006468516091 1103 42 1.062243918518739 1103 43 1.4055071474706793 1103 44 1.2271965157895508 1103 45 1.6551246469221397 1103 46 2.1659361225651352 1103 47 2.1507990281357294 1103 48 2.4308615710526453 1103 49 1.671503666862769 1103 50 2.0263654642348143 1103 51 1.433048355169177 2204 1 0.13984308762459108 2204 2 0.7007571968395254 2204 3 0.9716958618378939 2204 4 0.9102919476555726 2204 5 0.4820241883411569 2204 6 1.0009381399376878 2204 7 0.7796928118465019 2204 8 0.17520426167255465 2204 9 0.7045695516967925 2204 10 0.34255701321061593 2204 11 0.13054963285864574 2204 12 0.9495362468740964 2204 13 0.29409698034248777 2204 14 -0.04958730998197325 2204 15 1.1692652666990213 2204 16 1.4129035813329338 2204 17 0.4962918195716881 2204 18 0.6634755365931113 2204 19 1.2535964627905116 2204 20 0.19346301614871209 2204 21 0.49908157468222925 2204 22 1.1524655561755637 2204 23 0.39699837249351333 2204 24 0.45048536851814447 2204 25 0.5941069115485362 2204 26 0.6049425892284006 2204 27 0.8190727918602647 2204 28 0.11070276545544289 2204 29 0.9136514420234363 2204 30 0.8735831603898632 2204 31 1.1170682682222548 2204 32 1.4299537111892633 2204 33 0.6496165017287581 2204 34 0.6496310312232723 2204 35 1.295522364350525 2204 36 0.12530042079136194 2204 37 1.499791076432686 2204 38 0.3480283551814267 2204 39 1.3803224756953867 2204 40 1.187839679752983 2204 41 0.8464677688256828 2204 42 0.3966092435961386 2204 43 1.1702130298236213 2204 44 0.4578466705883102 2204 45 -0.015153990848759236 2204 46 1.042097113277208 2204 47 1.9619445389716432 2204 48 -1.0459186147058688 2204 49 -0.03747466131060406 2204 50 0.8452509131512258 2204 51 1.1141629062527656 2205 1 0.43998533513525473 2205 2 -0.16695261823871022 2205 3 -0.5674222035959602 2205 4 -0.43892569103575596 2205 5 0.3084822253255055 2205 6 -1.0260888870893368 2205 7 0.14882510203142374 2205 8 -0.9336150839888973 2205 9 -1.088460320280447 2205 10 -0.14037328550915618 2205 11 -0.5728643073973956 2205 12 -1.281615957962316 2205 13 -0.6233994634697453 2205 14 -0.3838689600530927 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-1.980668768331526 1213 24 -2.3325871777122984 1213 25 -1.6754521211683908 1213 26 -2.989652005366196 1213 27 -1.230002599320386 1213 28 -0.554304346920091 1213 29 -2.6709858268243636 1213 30 -2.6292617898235022 1213 31 -1.3530597545373482 1213 32 -1.6888229602189924 1213 33 -1.5402839250137745 1213 34 -1.130596564793791 1213 35 -1.9583894421928605 1213 36 -3.7580566204604153 1213 37 -1.962513333240139 1213 38 -2.092938927891119 1213 39 -0.5193930292832718 1213 40 -2.881150362921268 1213 41 -0.6905165839481384 1213 42 -0.09912333108380267 1213 43 -2.7081653485547577 1213 44 -2.3045999865951927 1213 45 -2.5706305200094945 1213 46 -1.8483723035080004 1213 47 -2.4676429432474194 1213 48 -1.9634150585181018 1213 49 -2.496222883216724 1213 50 -2.7550335818701157 1213 51 -1.4882553014286017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 1.0 20 2.0 21 6.0 22 13.0 23 21.0 24 44.0 25 68.0 26 104.0 27 192.0 28 258.0 29 311.0 30 388.0 31 475.0 32 565.0 33 665.0 34 808.0 35 1064.0 36 1489.0 37 2196.0 38 3728.0 39 10931.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 28.156755134416674 18.145178579085023 14.260601123354629 39.43746516314368 2 10.814865617900466 20.108020060868448 51.94821895494878 17.12889536628231 3 14.834340576914833 22.888003086022888 43.38862457674339 18.88903176031889 4 11.186832969011187 34.8034803480348 32.484677039132485 21.525009643821523 5 10.976811966910976 32.446101753032444 46.56037032274656 10.016715957310018 6 22.176503364622178 49.91213407054991 16.32591830611633 11.585444258711586 7 19.111911191119113 29.14148557712914 20.466332347520467 31.28027088423128 8 16.58737302301659 50.709356649950706 18.12181218121812 14.581458145814583 9 18.156101324418156 18.456131327418458 19.18906176331919 44.1987055848442 10 12.794136556512795 42.79285071364279 28.721443572928724 15.69156915691569 11 23.390484354907844 21.35876553793399 39.23703386198028 16.013716245177882 12 16.6002314517166 22.686554369722685 27.94993785092795 32.76327632763276 13 20.47061849042047 20.35060648922035 40.606917834640605 18.57185718571857 14 14.684325575414684 21.95791007672196 24.302430243024304 39.055334104839055 15 17.56889974711757 28.065663709228065 20.586344348720587 33.77909219493378 16 32.76327632763276 27.18700441472719 24.28957181432429 15.760147443315761 17 16.570228451416572 43.881531010243876 24.60674638892461 14.941494149414941 18 33.35047790493335 25.44683039732545 26.055462689126056 15.147229008615149 19 16.895975311816898 25.318246110325322 23.496635377823498 34.28914320003429 20 16.24019544811624 24.756761390424757 24.362436243624362 34.640606917834646 21 14.842912862714844 25.94830911662595 23.73237323732373 35.476404783335475 22 15.502978869315504 43.14002828854314 23.98954181132399 17.367451030817367 23 16.163044875916164 25.63113454202563 24.242424242424242 33.96339633963396 24 33.76194762333376 25.734001971625737 24.863914962924863 15.64013544211564 25 17.76606232051777 42.46710385324246 23.78809309502379 15.978740731215979 26 33.183318331833185 26.921263554926924 24.495306673524496 15.400111439715399 27 15.807295015215809 43.02001628734302 24.075264669324074 17.097424028117096 28 14.847199005614847 25.249667823925247 24.17813209892418 35.72500107153572 29 16.227337019416225 23.968111096823968 24.51245124512451 35.29210063863529 30 14.99056927297668 24.32270233196159 42.781207133058984 17.905521262002743 31 17.32887574471733 23.672367236723673 42.175646136042175 16.823110882516822 32 32.73755947023274 24.546740388324547 25.146800394325147 17.56889974711757 33 15.88015944451588 25.0996528224251 25.77686340062578 33.24332433243325 34 16.805966310916805 40.405469118340406 25.673995971025676 17.114568599717114 35 32.71612875573272 25.922592259225922 24.165273670224167 17.196005314817196 36 15.760147443315761 41.53701084394154 25.982598259825984 16.72024345291672 37 15.820153443915821 40.17401740174017 25.172517251725175 18.83331190261883 38 14.577172002914576 22.425099652822425 43.157172860143156 19.84055548411984 39 15.64013544211564 20.826368351120827 27.975654708327973 35.55784149843556 40 15.88015944451588 19.647679053619648 44.54588315974455 19.926278342119925 41 16.244481591016243 19.67768205391968 27.752775277527753 36.325061077536326 42 32.02177360593202 20.119154772620117 29.347220436329348 18.51185118511851 43 14.255711285414256 21.64930778792165 44.75161801894475 19.343362907719346 44 13.355621276413357 38.23668081093824 28.764305001928765 19.643392910719644 45 13.985684282713986 21.675024645321674 45.15451545154515 19.184775620419185 46 30.813081308130812 20.68063949252068 28.052805280528055 20.453473918820453 47 14.169988427414172 20.496335347820498 26.282628262826286 39.05104796193905 48 12.909862414812908 21.37928078522138 26.188333119026186 39.52252368093953 49 30.217307445030215 20.744931636020745 27.238438129527236 21.799322789421797 50 11.456859971711458 22.245081651022243 44.15155801294415 22.146500364322147 51 10.848227679910847 21.28927178432129 28.36997985512837 39.492520680639494 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 14.0 1 8.5 2 3.0 3 2.0 4 1.0 5 1.0 6 1.0 7 0.5 8 0.0 9 1.5 10 3.0 11 4.0 12 5.0 13 8.0 14 11.0 15 10.5 16 10.0 17 12.5 18 15.0 19 11.0 20 7.0 21 8.0 22 9.0 23 8.5 24 8.0 25 10.5 26 15.5 27 18.0 28 46.5 29 75.0 30 213.0 31 351.0 32 613.0 33 875.0 34 1415.0 35 1955.0 36 2577.0 37 3199.0 38 3427.5 39 3656.0 40 3348.0 41 3040.0 42 2516.5 43 1993.0 44 1617.5 45 1242.0 46 932.0 47 622.0 48 535.0 49 448.0 50 660.0 51 872.0 52 2608.0 53 4344.0 54 2260.0 55 176.0 56 143.5 57 111.0 58 99.0 59 87.0 60 71.5 61 56.0 62 44.0 63 32.0 64 30.5 65 29.0 66 23.0 67 17.0 68 18.5 69 20.0 70 13.0 71 6.0 72 5.0 73 4.0 74 3.0 75 1.5 76 1.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03428914320003429 2 0.008572285800008572 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.004286142900004286 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.012858428700012858 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 23331.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.34917171318284 #Duplication Level Percentage of deduplicated Percentage of total 1 92.72132381423738 67.0831097810989 2 5.865368724542881 8.487091380261704 3 0.7457359001113205 1.618601240695167 4 0.24163686783085386 0.6992890897172046 5 0.13765016406383454 0.49794376781010774 6 0.048938087698862105 0.21243780661438658 7 0.0362635549544697 0.18365467150319598 8 0.04803815212098478 0.27804164132681014 9 0.011967365753866614 0.07792460998928635 >10 0.11924244051772911 1.6861631150724001 >50 0.005958732041949327 0.31470980921796066 >100 0.011917464083898655 1.8149702695994716 >500 0.0 0.0 >1k 0.005958732041949327 17.04606281709338 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC 3954 16.947409026616945 No Hit TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT 241 1.032960438901033 RNA PCR Primer, Index 40 (95% over 21bp) TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT 180 0.7715057220007715 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG 73 0.3128884317003129 No Hit GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC 41 0.17573185890017573 No Hit AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC 36 0.1543011444001543 No Hit AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC 33 0.14144271570014144 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 32 0.13715657280013716 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 31 0.13287042990013287 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT 26 0.11143971540011144 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.004286142900004286 2 0.0 0.0 0.008572285800008572 3 0.0 0.0 0.012858428700012858 4 0.0 0.0 0.012858428700012858 5 0.0 0.0 0.017144571600017144 6 0.0 0.0 0.017144571600017144 7 0.0 0.0 0.02143071450002143 8 0.0 0.0 0.025716857400025717 9 0.0 0.0 0.030003000300030003 10 0.0 0.0 0.038575286100038575 11 0.0 0.0 0.04714757190004715 12 0.0 0.0 0.05571985770005572 13 0.0 0.0 0.07286442930007286 14 0.0 0.0 0.07715057220007715 15 0.0 0.0 0.12858428700012858 16 0.0 0.0 0.14572885860014573 17 0.0 0.0 0.1885902876001886 18 0.0 0.0 0.22716557370022716 19 0.0 0.0 0.2485962882002486 20 0.0 0.0 0.2785992885002786 21 0.0 0.0 0.3086022888003086 22 0.0 0.0 0.38146671810038146 23 0.0 0.0 0.7200720072007201 24 0.0 0.0 0.9429514380009429 25 0.0 0.0 0.9772405812009772 26 0.0 0.0 1.2901290129012901 27 0.0 0.0 1.3887102996013887 28 0.0 0.0 1.5687283014015687 29 0.0 0.0 1.7573185890017573 30 0.0 0.0 1.963053448201963 31 0.0 0.0 2.498821310702499 32 0.0 0.0 3.3903390339033903 33 0.0 0.0 4.4875916163044876 34 0.0 0.0 5.786292915005786 35 0.0 0.0 7.230723072307231 36 0.0 0.0 8.7008700870087 37 0.0 0.0 10.565342248510566 38 0.0 0.0 12.596973983112598 39 0.0 0.0 14.564313574214564 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCTCG 20 6.893415E-4 44.997856 36 CGAGACG 20 6.893415E-4 44.997856 23 GAGACGA 20 6.893415E-4 44.997856 24 GCCGTCT 50 9.877112E-10 40.49807 45 TGCCGTC 475 0.0 40.26124 45 ATCTCGT 510 0.0 38.380524 37 TCTCGTA 510 0.0 38.380524 38 CTCGTAT 510 0.0 38.380524 39 CCTTGCA 495 0.0 38.179996 28 GCAGAAT 495 0.0 38.179996 32 ACCTTGC 495 0.0 38.179996 27 AATCTCG 490 0.0 38.110428 36 CCACGAG 520 0.0 38.075108 20 CACGAGA 520 0.0 38.075108 21 TATGCCG 515 0.0 38.007896 43 CCCACGA 515 0.0 38.007896 19 CGTATGC 515 0.0 38.007896 41 ATGCCGT 510 0.0 37.93937 44 TGCAGAA 505 0.0 37.869484 31 TTGCAGA 500 0.0 37.7982 30 >>END_MODULE