FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_30.341000000069f5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_30.341000000069f5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23331
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC395416.947409026616945No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT2411.032960438901033RNA PCR Primer, Index 40 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGT1800.7715057220007715No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCG730.3128884317003129No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC410.17573185890017573No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC360.1543011444001543No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC330.14144271570014144No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC320.13715657280013716No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA310.13287042990013287No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT260.11143971540011144No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG206.893415E-444.99785636
CGAGACG206.893415E-444.99785623
GAGACGA206.893415E-444.99785624
GCCGTCT509.877112E-1040.4980745
TGCCGTC4750.040.2612445
ATCTCGT5100.038.38052437
TCTCGTA5100.038.38052438
CTCGTAT5100.038.38052439
CCTTGCA4950.038.17999628
GCAGAAT4950.038.17999632
ACCTTGC4950.038.17999627
AATCTCG4900.038.11042836
CCACGAG5200.038.07510820
CACGAGA5200.038.07510821
TATGCCG5150.038.00789643
CCCACGA5150.038.00789619
CGTATGC5150.038.00789641
ATGCCGT5100.037.9393744
TGCAGAA5050.037.86948431
TTGCAGA5000.037.798230