##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_3.34100000006849.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35280 Sequences flagged as poor quality 0 Sequence length 51 %GC 24 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.429620181405895 38.0 38.0 38.0 32.0 38.0 2 36.32423469387755 38.0 38.0 38.0 32.0 38.0 3 36.24852607709751 38.0 38.0 38.0 32.0 38.0 4 36.29957482993197 38.0 38.0 38.0 32.0 38.0 5 36.359948979591834 38.0 38.0 38.0 32.0 38.0 6 38.2656462585034 40.0 38.0 40.0 38.0 40.0 7 38.210204081632654 40.0 38.0 40.0 38.0 40.0 8 38.13543083900227 40.0 38.0 40.0 38.0 40.0 9 38.16904761904762 40.0 38.0 40.0 38.0 40.0 10 38.0781179138322 40.0 38.0 40.0 32.0 40.0 11 38.027012471655325 40.0 38.0 40.0 32.0 40.0 12 37.99744897959184 40.0 38.0 40.0 32.0 40.0 13 37.949716553287985 40.0 38.0 40.0 32.0 40.0 14 37.87103174603175 40.0 38.0 40.0 32.0 40.0 15 37.824574829931976 40.0 38.0 40.0 32.0 40.0 16 37.787273242630384 40.0 38.0 40.0 32.0 40.0 17 37.88667800453515 40.0 38.0 40.0 32.0 40.0 18 37.845181405895694 40.0 38.0 40.0 32.0 40.0 19 37.99438775510204 40.0 38.0 40.0 32.0 40.0 20 38.021145124716554 40.0 38.0 40.0 32.0 40.0 21 38.070436507936506 40.0 38.0 40.0 32.0 40.0 22 38.147704081632654 40.0 38.0 40.0 38.0 40.0 23 38.06726190476191 40.0 38.0 40.0 32.0 40.0 24 38.09727891156463 40.0 38.0 40.0 32.0 40.0 25 38.1296201814059 40.0 38.0 40.0 32.0 40.0 26 38.022392290249435 40.0 38.0 40.0 32.0 40.0 27 37.9234126984127 40.0 38.0 40.0 32.0 40.0 28 37.82080498866213 40.0 38.0 40.0 32.0 40.0 29 37.74569160997732 40.0 38.0 40.0 32.0 40.0 30 37.658815192743766 40.0 38.0 40.0 32.0 40.0 31 37.5594387755102 40.0 38.0 40.0 32.0 40.0 32 37.45334467120181 40.0 38.0 40.0 32.0 40.0 33 37.23956916099773 40.0 38.0 40.0 32.0 40.0 34 37.11604308390023 40.0 38.0 40.0 32.0 40.0 35 36.9531179138322 40.0 38.0 40.0 32.0 40.0 36 36.701445578231294 40.0 38.0 40.0 27.0 40.0 37 36.47984693877551 40.0 38.0 40.0 27.0 40.0 38 36.20532879818594 40.0 38.0 40.0 27.0 40.0 39 35.863973922902495 40.0 38.0 40.0 27.0 40.0 40 35.51374716553288 40.0 38.0 40.0 13.0 40.0 41 35.10340136054422 40.0 38.0 40.0 13.0 40.0 42 34.65558390022676 40.0 32.0 40.0 13.0 40.0 43 34.10337301587302 40.0 32.0 40.0 13.0 40.0 44 33.5375 38.0 32.0 40.0 13.0 40.0 45 32.942233560090706 38.0 27.0 40.0 13.0 40.0 46 32.30538548752835 38.0 27.0 40.0 13.0 40.0 47 31.66890589569161 38.0 27.0 40.0 13.0 40.0 48 31.06108276643991 38.0 13.0 40.0 13.0 40.0 49 30.378826530612244 38.0 13.0 40.0 13.0 40.0 50 29.817545351473925 38.0 13.0 40.0 13.0 40.0 51 29.209013605442177 38.0 13.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.20675492299085363 2201 2 1.0623459022382065 2201 3 0.7287059377513501 2201 4 1.0783790154021418 2201 5 0.7680633320307066 2201 6 1.1994433515648737 2201 7 0.810932173435944 2201 8 1.1825599733000942 2201 9 1.1433452342083967 2201 10 1.0501601872499933 2201 11 1.0263562428172293 2201 12 1.334494628690699 2201 13 0.32529601934740526 2201 14 0.4327297933089227 2201 15 1.3803196569343683 2201 16 0.42563406526805636 2201 17 -0.0312546499025288 2201 18 0.891807475121837 2201 19 0.7037297270165865 2201 20 0.7072527151401076 2201 21 0.36105563601231694 2201 22 0.792427094587616 2201 23 -0.4022864450522903 2201 24 1.0758850082422953 2201 25 0.6798420007503623 2201 26 1.1468289537384067 2201 27 1.6573684830559188 2201 28 1.537739617968569 2201 29 0.808618540859058 2201 30 1.3427939499792458 2201 31 0.5034781471681811 2201 32 -0.23228085307718516 2201 33 1.4504569979071675 2201 34 0.9087620755859902 2201 35 0.35123029181171006 2201 36 0.6576350632847223 2201 37 1.6788963702817696 2201 38 1.2527130713124137 2201 39 0.7742557862950648 2201 40 0.5912184066649004 2201 41 1.0496861975610656 2201 42 0.5508786927707021 2201 43 0.1260925070981429 2201 44 1.3055022788656032 2201 45 0.6852464242242036 2201 46 0.9905596453818717 2201 47 1.5516845369717984 2201 48 1.5595898147896747 2201 49 0.7593332508871953 2201 50 1.9523635545117877 2201 51 1.5659057343306984 1101 1 -0.05411464222653706 1101 2 -0.35069757602266094 1101 3 0.20696680731656159 1101 4 -0.48683837590220946 1101 5 0.1376285494220113 1101 6 -0.452730561478603 1101 7 0.28919304300116266 1101 8 -0.1435269832216477 1101 9 0.5129104515997014 1101 10 0.593638448119556 1101 11 0.7002692862954873 1101 12 0.2692772373863548 1101 13 -0.3051387632612901 1101 14 0.171860228091532 1101 15 0.010754439543063654 1101 16 0.056068847876751704 1101 17 0.012223610967033949 1101 18 -0.4560186118346863 1101 19 -0.5788789686355855 1101 20 -0.07535598051206449 1101 21 0.03496867949058213 1101 22 0.9011227467615299 1101 23 0.7281483375564051 1101 24 -0.05454977436640007 1101 25 0.3320159137938461 1101 26 1.1903072146079694 1101 27 0.5921510917515747 1101 28 0.4942613570990062 1101 29 0.46079245390253476 1101 30 1.3210548195444645 1101 31 0.5686955384725323 1101 32 0.7677191469228148 1101 33 0.4939352587767374 1101 34 0.19137077123816226 1101 35 1.1555781178986706 1101 36 1.6141568024151596 1101 37 1.3962876746295976 1101 38 1.1657565495732811 1101 39 0.9046905689037601 1101 40 0.8738271023170796 1101 41 1.9844688062567215 1101 42 1.9204439101620068 1101 43 0.23478815927205687 1101 44 -1.5205846776561351 1101 45 -0.9669274888192732 1101 46 -1.2050925285311678 1101 47 -1.4917937238977679 1101 48 -1.788236272166845 1101 49 -2.979797183895414 1101 50 -2.8302451411403844 1101 51 -3.781920352625825 2202 1 -0.3782692315985159 2202 2 -0.08499709293087676 2202 3 0.061073087509804225 2202 4 -0.2694470715543815 2202 5 0.747773476958244 2202 6 0.0777042211300909 2202 7 -1.1881016429891744 2202 8 -1.365266113656432 2202 9 0.12788629701033472 2202 10 -0.8807576871461436 2202 11 -0.7494891678107933 2202 12 -1.8365198640629217 2202 13 -2.5805010821018683 2202 14 -2.1614731052418037 2202 15 -0.3269267198772212 2202 16 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1215 48 -1.595504279953996 1215 49 -0.8688302792880158 1215 50 -1.216682519466147 1215 51 -2.059013149510964 1212 1 -0.2540433663890411 1212 2 -0.5039406266285056 1212 3 -0.08027481777252632 1212 4 -0.5481355961445473 1212 5 -0.007417779872362473 1212 6 -0.10918102477154434 1212 7 -0.5276280546467333 1212 8 0.09702896831078078 1212 9 -0.04624849351790061 1212 10 -0.4683715304976914 1212 11 -0.25732159039731073 1212 12 -0.019033525265108153 1212 13 0.28288689604020334 1212 14 -0.23797583748223872 1212 15 -0.1214622390029092 1212 16 0.2410296461661332 1212 17 -0.10858893358036426 1212 18 0.8301538756920408 1212 19 -0.2374677070532627 1212 20 -0.1683709484379392 1212 21 0.5278410957414721 1212 22 0.14132231910650006 1212 23 0.639409919879995 1212 24 0.043810881371307175 1212 25 0.2525433549912748 1212 26 -0.29508123870635217 1212 27 -0.17584605721492608 1212 28 0.0038835951603033436 1212 29 0.1892315130614861 1212 30 0.818203786044819 1212 31 -0.09737716288170617 1212 32 0.9658659751480485 1212 33 1.071625921642024 1212 34 1.0837442566266162 1212 35 1.5393985027881953 1212 36 1.5279130390509366 1212 37 1.3300011457628855 1212 38 1.928051631540491 1212 39 2.0397582809493713 1212 40 1.5313467031723889 1212 41 2.372922120583162 1212 42 0.9343426984727685 1212 43 1.3014310673262202 1212 44 2.0082820365277527 1212 45 1.7208843429697467 1212 46 1.8330400445265624 1212 47 0.7270230971998757 1212 48 1.8617993657518994 1212 49 1.6880574133961055 1212 50 0.9719644098218403 1212 51 0.8695408020427458 1213 1 0.05338982471807441 1213 2 -0.37571246583802775 1213 3 0.14204721231953954 1213 4 -0.06396761949958574 1213 5 -1.0586192171056865 1213 6 -1.2401039980479922 1213 7 -1.0407652655158088 1213 8 -0.3159510451632812 1213 9 -0.6952491672210215 1213 10 -0.8530560128691249 1213 11 -0.5811482241273822 1213 12 -0.7095792247935151 1213 13 -1.0234234565310913 1213 14 -1.6363589499906581 1213 15 -0.8757851673658053 1213 16 -0.6821681979838843 1213 17 -0.6462040245302845 1213 18 -0.1132550621027022 1213 19 -0.26261929025559994 1213 20 -0.5330689048718042 1213 21 -0.8456150012717814 1213 22 -0.274577074560689 1213 23 0.36453904631162004 1213 24 -0.21893333601023812 1213 25 -0.5080674691722109 1213 26 0.564339376609162 1213 27 -0.15204188025557386 1213 28 0.12380275078572822 1213 29 -0.22711701602003842 1213 30 0.6989583335408867 1213 31 0.8983560506821817 1213 32 0.6301372529382974 1213 33 0.2970918996343883 1213 34 0.24318732871284965 1213 35 0.3548038474996247 1213 36 0.999505224094726 1213 37 1.006174512032814 1213 38 1.3369894262856121 1213 39 0.42017597688469976 1213 40 1.2737675430556124 1213 41 -0.006597304523509706 1213 42 0.6920341424431271 1213 43 0.9158483141261371 1213 44 0.27214909244511176 1213 45 0.45117852666613345 1213 46 0.9399342731364868 1213 47 -0.8777377381919926 1213 48 0.2689406188397072 1213 49 -0.06020218687337575 1213 50 0.721571416334303 1213 51 -0.7390376843709099 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 3.0 17 2.0 18 3.0 19 2.0 20 9.0 21 19.0 22 37.0 23 49.0 24 114.0 25 160.0 26 288.0 27 468.0 28 578.0 29 751.0 30 992.0 31 1223.0 32 1499.0 33 1902.0 34 2327.0 35 2721.0 36 2911.0 37 3410.0 38 4362.0 39 11449.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.70428125886022 24.743407995463567 14.017578678763822 12.53473206691239 2 9.4359410430839 29.971655328798185 31.00907029478458 29.583333333333332 3 30.62074829931973 36.19614512471656 25.71428571428571 7.4688208616780045 4 15.189909297052154 20.960884353741495 34.10714285714286 29.74206349206349 5 8.098072562358277 32.3469387755102 40.89285714285714 18.66213151927438 6 37.5 31.845238095238095 10.731292517006803 19.9234693877551 7 8.381519274376418 39.48129251700681 26.907596371882086 25.229591836734695 8 21.52777777777778 23.761337868480727 31.11111111111111 23.599773242630384 9 16.819727891156464 27.721088435374146 25.1672335600907 30.291950113378686 10 12.23639455782313 25.232426303854876 31.35487528344671 31.176303854875282 11 20.472262380587917 23.916432803242905 18.385917169827366 37.22538764634181 12 18.4297052154195 35.23242630385487 29.209183673469386 17.128684807256235 13 25.810657596371883 21.570294784580497 27.06916099773243 25.549886621315192 14 17.47448979591837 26.318027210884352 35.54988662131519 20.657596371882086 15 31.768707482993197 20.308956916099774 31.743197278911566 16.179138321995463 16 10.267021940019276 32.80514768410907 27.161403707693182 29.766426668178468 17 13.616780045351472 33.51757369614513 36.901927437641724 15.963718820861677 18 12.562714362652002 24.232546273987356 49.071685705377135 14.133053657983503 19 8.673469387755102 38.9172335600907 38.29648526077097 14.112811791383221 20 18.89455782312925 24.95748299319728 41.88208616780045 14.265873015873016 21 3.293650793650794 33.939909297052154 42.709750566893426 20.056689342403626 22 5.467687074829932 41.78004535147392 42.817460317460316 9.934807256235828 23 3.36734693877551 34.60600907029479 46.967120181405896 15.059523809523808 24 4.41609977324263 41.98696145124717 47.64172335600907 5.955215419501133 25 9.773242630385488 36.284013605442176 45.031179138321995 8.91156462585034 26 3.959750566893424 35.58956916099773 51.01473922902494 9.4359410430839 27 6.964285714285714 41.03458049886621 49.475623582766445 2.5255102040816326 28 3.3758503401360542 37.874149659863946 49.804421768707485 8.945578231292517 29 3.7528344671201816 39.66836734693878 51.57596371882086 5.002834467120181 30 5.6407494543496135 39.760197284503526 51.0955525950282 3.5035006661186543 31 3.7641723356009074 40.17573696145125 47.933673469387756 8.12641723356009 32 5.459183673469388 41.30952380952381 49.0702947845805 4.160997732426304 33 4.087301587301587 41.75170068027211 47.95068027210884 6.2103174603174605 34 4.220521541950113 44.54081632653061 47.09183673469388 4.146825396825396 35 3.8747165532879815 45.29478458049886 45.924036281179134 4.906462585034014 36 3.758503401360544 45.821995464852606 46.624149659863946 3.7953514739229024 37 3.8548752834467117 47.48299319727891 45.532879818594104 3.1292517006802725 38 4.291383219954648 45.416666666666664 45.92120181405896 4.370748299319728 39 4.611678004535148 45.479024943310655 45.08503401360544 4.824263038548753 40 5.087868480725623 45.0453514739229 45.102040816326536 4.7647392290249435 41 5.357142857142857 45.317460317460316 43.35317460317461 5.972222222222222 42 7.644557823129252 44.243197278911566 43.146258503401356 4.965986394557823 43 6.51360544217687 44.69954648526077 43.217120181405896 5.569727891156463 44 7.366780045351475 44.849773242630384 41.98412698412699 5.799319727891157 45 7.551020408163265 43.68764172335601 41.88208616780045 6.879251700680272 46 9.914965986394558 43.5062358276644 39.299886621315196 7.278911564625851 47 8.942743764172336 42.85430839002268 38.75850340136054 9.444444444444445 48 9.654195011337869 42.39229024943311 38.149092970521544 9.804421768707483 49 11.732191955554296 42.15538989200374 37.328155559964856 8.784262592477111 50 11.425736961451246 40.90702947845805 37.763605442176875 9.903628117913833 51 12.951047365288131 40.718274327503615 34.966977522038604 11.363700785169648 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 205.0 1 150.0 2 95.0 3 211.5 4 328.0 5 369.5 6 411.0 7 292.0 8 173.0 9 381.5 10 590.0 11 748.0 12 906.0 13 1226.5 14 1547.0 15 2350.5 16 3154.0 17 3088.0 18 3022.0 19 2851.5 20 2681.0 21 2522.0 22 2363.0 23 3570.5 24 4778.0 25 3396.5 26 2395.5 27 2776.0 28 3508.0 29 4240.0 30 2772.5 31 1305.0 32 1056.0 33 807.0 34 722.5 35 638.0 36 513.5 37 389.0 38 316.5 39 244.0 40 280.5 41 317.0 42 299.5 43 282.0 44 271.0 45 260.0 46 285.0 47 310.0 48 396.0 49 482.0 50 570.0 51 658.0 52 400.0 53 142.0 54 92.0 55 42.0 56 39.0 57 36.0 58 23.0 59 10.0 60 12.0 61 14.0 62 12.5 63 11.0 64 10.0 65 9.0 66 9.0 67 9.0 68 9.0 69 9.0 70 7.0 71 5.0 72 6.5 73 8.0 74 6.0 75 3.0 76 2.0 77 1.5 78 1.0 79 0.5 80 0.0 81 1.0 82 2.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02834467120181406 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.008503401360544217 12 0.0 13 0.0 14 0.0 15 0.0 16 0.005668934240362812 17 0.0 18 0.002834467120181406 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.002834467120181406 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.002834467120181406 50 0.0 51 0.002834467120181406 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 35280.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.1215685442177 #Duplication Level Percentage of deduplicated Percentage of total 1 86.75283330111876 31.336484144914532 2 6.166825246193024 4.4551080166114705 3 2.2127840948036117 2.3978769706201013 4 1.3174084771281094 1.9034744242926585 5 0.7622901538390937 1.376755802124054 6 0.4966406987915623 1.0763664625948557 7 0.34582455237614607 0.8744207691049855 8 0.24334780527714636 0.7032083542722708 9 0.2039935447389129 0.6631710127978124 >10 1.0666839552165999 7.629470734030057 >50 0.15686115291526345 3.9010898056676244 >100 0.19607644114407932 17.224855496339256 >500 0.0549014035203422 14.108516508514718 >1k 0.023529172937289518 12.349201498115594 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1889 5.354308390022676 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1469 4.163832199546485 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA 1001 2.8373015873015874 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 851 2.4121315192743764 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 779 2.208049886621315 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 725 2.054988662131519 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA 699 1.9812925170068028 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA 695 1.969954648526077 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 638 1.808390022675737 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 593 1.6808390022675737 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 489 1.3860544217687076 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 479 1.3577097505668934 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 428 1.2131519274376417 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 422 1.1961451247165533 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 413 1.1706349206349207 RNA PCR Primer, Index 27 (95% over 21bp) CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 385 1.0912698412698412 No Hit GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 356 1.0090702947845804 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 308 0.873015873015873 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 296 0.8390022675736961 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 260 0.7369614512471655 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 218 0.6179138321995464 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 205 0.5810657596371882 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 202 0.572562358276644 No Hit ATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 181 0.5130385487528344 No Hit GTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAA 179 0.5073696145124716 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAA 158 0.4478458049886621 No Hit GCGTTGATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTT 148 0.41950113378684806 No Hit GCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 139 0.3939909297052154 No Hit TTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 124 0.35147392290249435 No Hit CTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 122 0.34580498866213155 No Hit TTGCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 119 0.3373015873015873 No Hit CTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAA 117 0.33163265306122447 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 112 0.31746031746031744 No Hit AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 112 0.31746031746031744 No Hit AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAA 108 0.30612244897959184 No Hit GCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT 100 0.2834467120181406 No Hit CTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 98 0.2777777777777778 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92 0.26077097505668934 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 90 0.25510204081632654 No Hit AAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAA 87 0.24659863945578228 No Hit ATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTT 84 0.2380952380952381 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 75 0.21258503401360546 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 74 0.20975056689342403 No Hit GGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 65 0.18424036281179137 No Hit CGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 64 0.18140589569160998 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62 0.17573696145124718 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 62 0.17573696145124718 No Hit TTTTTTGCCCGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 56 0.15873015873015872 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAGCAGTGGTATCAACG 54 0.15306122448979592 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG 54 0.15306122448979592 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 53 0.15022675736961452 No Hit CTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT 53 0.15022675736961452 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 52 0.14739229024943312 No Hit CGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 51 0.1445578231292517 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51 0.1445578231292517 No Hit GCTTCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT 48 0.13605442176870747 No Hit GATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT 48 0.13605442176870747 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.13321995464852607 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.13321995464852607 No Hit TGATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT 45 0.12755102040816327 No Hit TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT 42 0.11904761904761905 RNA PCR Primer, Index 38 (95% over 21bp) GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATA 42 0.11904761904761905 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT 41 0.11621315192743764 No Hit ACTCTGCGTTGATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTA 39 0.11054421768707484 No Hit AAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAA 38 0.10770975056689341 No Hit GCGTTGATACCACTGCTTGCTTGCGAAAAAAAAAAAAAAAAAAAAAAAAAA 37 0.10487528344671201 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.002834467120181406 10 0.0 0.0 0.002834467120181406 11 0.0 0.0 0.002834467120181406 12 0.0 0.0 0.008503401360544218 13 0.0 0.0 0.011337868480725623 14 0.0 0.0 0.01417233560090703 15 0.0 0.0 0.022675736961451247 16 0.0 0.0 0.036848072562358274 17 0.0 0.0 0.053854875283446714 18 0.0 0.0 0.06802721088435375 19 0.0 0.0 0.08786848072562359 20 0.0 0.0 0.0992063492063492 21 0.0 0.0 0.11621315192743764 22 0.0 0.0 0.15022675736961452 23 0.0 0.0 0.17290249433106575 24 0.0 0.0 0.21258503401360543 25 0.0 0.0 0.2494331065759637 26 0.0 0.0 0.272108843537415 27 0.0 0.0 0.2891156462585034 28 0.0 0.0 0.31746031746031744 29 0.0 0.0 0.3713151927437642 30 0.0 0.0 0.41950113378684806 31 0.0 0.0 0.5272108843537415 32 0.0 0.0 0.6689342403628118 33 0.0 0.0 0.7426303854875284 34 0.0 0.0 0.9240362811791383 35 0.0 0.0 1.0232426303854876 36 0.0 0.0 1.1564625850340136 37 0.0 0.0 1.2925170068027212 38 0.0 0.0 1.3775510204081634 39 0.0 0.0 1.5419501133786848 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTATCT 45 3.3968106E-5 30.0 34 ATTGCTA 45 3.3968106E-5 30.0 31 CGAATTG 45 3.3968106E-5 30.0 28 GAATTGC 45 3.3968106E-5 30.0 29 TGCTATC 45 3.3968106E-5 30.0 33 AATTGCT 45 3.3968106E-5 30.0 30 TTGCTAT 45 3.3968106E-5 30.0 32 GTATGCC 50 6.966832E-5 27.0 42 ATCTCGT 50 6.966832E-5 27.0 37 TGCCGTC 50 6.966832E-5 27.0 45 TATGCCG 50 6.966832E-5 27.0 43 TCTCGTA 50 6.966832E-5 27.0 38 TATCTCG 50 6.966832E-5 27.0 36 AGACGAA 50 6.966832E-5 27.0 25 ATGCCGT 50 6.966832E-5 27.0 44 CGTATGC 50 6.966832E-5 27.0 41 GACGAAT 50 6.966832E-5 27.0 26 TCGTATG 50 6.966832E-5 27.0 40 ACGAATT 50 6.966832E-5 27.0 27 CTCGTAT 50 6.966832E-5 27.0 39 >>END_MODULE