FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_3.34100000006849.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_3.34100000006849.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35280
Sequences flagged as poor quality0
Sequence length51
%GC24

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18895.354308390022676No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14694.163832199546485No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA10012.8373015873015874No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8512.4121315192743764No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7792.208049886621315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7252.054988662131519No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA6991.9812925170068028No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA6951.969954648526077No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6381.808390022675737No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5931.6808390022675737No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4891.3860544217687076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4791.3577097505668934No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4281.2131519274376417No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4221.1961451247165533No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC4131.1706349206349207RNA PCR Primer, Index 27 (95% over 21bp)
CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3851.0912698412698412No Hit
GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3561.0090702947845804No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3080.873015873015873No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2960.8390022675736961No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2600.7369614512471655No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.6179138321995464No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2050.5810657596371882No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2020.572562358276644No Hit
ATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1810.5130385487528344No Hit
GTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAA1790.5073696145124716No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAA1580.4478458049886621No Hit
GCGTTGATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTT1480.41950113378684806No Hit
GCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1390.3939909297052154No Hit
TTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.35147392290249435No Hit
CTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.34580498866213155No Hit
TTGCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT1190.3373015873015873No Hit
CTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAA1170.33163265306122447No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1120.31746031746031744No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1120.31746031746031744No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAA1080.30612244897959184No Hit
GCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT1000.2834467120181406No Hit
CTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.2777777777777778No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.26077097505668934No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.25510204081632654No Hit
AAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAA870.24659863945578228No Hit
ATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTT840.2380952380952381No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG750.21258503401360546No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA740.20975056689342403No Hit
GGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA650.18424036281179137No Hit
CGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT640.18140589569160998No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT620.17573696145124718No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA620.17573696145124718No Hit
TTTTTTGCCCGCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.15873015873015872No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCGCAAGCAGTGGTATCAACG540.15306122448979592No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG540.15306122448979592No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG530.15022675736961452No Hit
CTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT530.15022675736961452No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA520.14739229024943312No Hit
CGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.1445578231292517No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.1445578231292517No Hit
GCTTCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT480.13605442176870747No Hit
GATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT480.13605442176870747No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.13321995464852607No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.13321995464852607No Hit
TGATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTT450.12755102040816327No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT420.11904761904761905RNA PCR Primer, Index 38 (95% over 21bp)
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATA420.11904761904761905No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAT410.11621315192743764No Hit
ACTCTGCGTTGATACCACTGCTTGCGCAAGCAGTGGTATCAACGCAGAGTA390.11054421768707484No Hit
AAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAA380.10770975056689341No Hit
GCGTTGATACCACTGCTTGCTTGCGAAAAAAAAAAAAAAAAAAAAAAAAAA370.10487528344671201No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATCT453.3968106E-530.034
ATTGCTA453.3968106E-530.031
CGAATTG453.3968106E-530.028
GAATTGC453.3968106E-530.029
TGCTATC453.3968106E-530.033
AATTGCT453.3968106E-530.030
TTGCTAT453.3968106E-530.032
GTATGCC506.966832E-527.042
ATCTCGT506.966832E-527.037
TGCCGTC506.966832E-527.045
TATGCCG506.966832E-527.043
TCTCGTA506.966832E-527.038
TATCTCG506.966832E-527.036
AGACGAA506.966832E-527.025
ATGCCGT506.966832E-527.044
CGTATGC506.966832E-527.041
GACGAAT506.966832E-527.026
TCGTATG506.966832E-527.040
ACGAATT506.966832E-527.027
CTCGTAT506.966832E-527.039
Produced by FastQC (version 0.11.2)