FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_28.341000000069db.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_28.341000000069db.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158550
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC12630.7965941343424787No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA3040.19173762220119836No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT2260.1425417849258909No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA1830.11542100283822138No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC1660.10469883317565437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1800.042.49910738
AATCTCG1750.042.42767736
GCCCACG1900.041.44649518
TATGCCG1900.041.44649543
CCCACGA1900.041.44649519
CCGAGCC1900.041.44649514
CGAGCCC1900.041.44649515
TGCCGTC1900.041.44649545
ATCTCGT1850.041.35048337
CTCGTAT1850.041.35048339
ACGAGAC1950.040.38376622
CCACGAG1950.040.38376620
TCGTATG1900.040.2623140
CGAGACC1900.040.2623123
CTCCGAG2050.039.51136412
TCCGAGC2000.039.37417213
GTATGCC2000.039.37417242
ATCTCCG2050.038.41382610
CGTATGC2050.038.41382641
TCTCCGA2050.038.41382611
Produced by FastQC (version 0.11.2)