##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_27.341000000069ce.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 84285 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.299412706887345 38.0 38.0 38.0 32.0 38.0 2 36.35098772023492 38.0 38.0 38.0 32.0 38.0 3 36.302651717387434 38.0 38.0 38.0 32.0 38.0 4 36.38611852642819 38.0 38.0 38.0 32.0 38.0 5 36.43827490063475 38.0 38.0 38.0 32.0 38.0 6 38.30807379723557 40.0 38.0 40.0 38.0 40.0 7 38.22371715014534 40.0 38.0 40.0 38.0 40.0 8 38.16883193925372 40.0 38.0 40.0 38.0 40.0 9 38.22396630479919 40.0 38.0 40.0 38.0 40.0 10 38.11914338257104 40.0 38.0 40.0 38.0 40.0 11 38.104241561369165 40.0 38.0 40.0 38.0 40.0 12 37.98188289731269 40.0 38.0 40.0 32.0 40.0 13 38.0787565996322 40.0 38.0 40.0 38.0 40.0 14 37.96437088449902 40.0 38.0 40.0 32.0 40.0 15 37.99953728421427 40.0 38.0 40.0 32.0 40.0 16 37.99136263866643 40.0 38.0 40.0 32.0 40.0 17 37.99282197306757 40.0 38.0 40.0 32.0 40.0 18 38.024773091297384 40.0 38.0 40.0 32.0 40.0 19 38.05114789108382 40.0 38.0 40.0 38.0 40.0 20 38.054280121017975 40.0 38.0 40.0 38.0 40.0 21 38.06746158865753 40.0 38.0 40.0 38.0 40.0 22 38.0327104466987 40.0 38.0 40.0 32.0 40.0 23 38.03669692116035 40.0 38.0 40.0 32.0 40.0 24 38.018330663819185 40.0 38.0 40.0 32.0 40.0 25 38.013952660615765 40.0 38.0 40.0 32.0 40.0 26 37.94708429732455 40.0 38.0 40.0 32.0 40.0 27 37.93997745743608 40.0 38.0 40.0 32.0 40.0 28 37.912036542682564 40.0 38.0 40.0 32.0 40.0 29 37.93421130687548 40.0 38.0 40.0 32.0 40.0 30 37.90137035059619 40.0 38.0 40.0 32.0 40.0 31 37.913946728362106 40.0 38.0 40.0 32.0 40.0 32 37.87972948923296 40.0 38.0 40.0 32.0 40.0 33 37.86567004805126 40.0 38.0 40.0 32.0 40.0 34 37.89388384647327 40.0 38.0 40.0 32.0 40.0 35 37.89620928990924 40.0 38.0 40.0 32.0 40.0 36 37.85009194993178 40.0 38.0 40.0 32.0 40.0 37 37.84890549919915 40.0 38.0 40.0 32.0 40.0 38 37.84520377291333 40.0 38.0 40.0 32.0 40.0 39 37.814320460342884 40.0 38.0 40.0 32.0 40.0 40 37.783745624962926 40.0 38.0 40.0 32.0 40.0 41 37.80624073085365 40.0 38.0 40.0 32.0 40.0 42 37.7917541674082 40.0 38.0 40.0 32.0 40.0 43 37.824666310731445 40.0 38.0 40.0 32.0 40.0 44 37.757216586581244 40.0 38.0 40.0 32.0 40.0 45 37.7421012042475 40.0 38.0 40.0 32.0 40.0 46 37.67995491487216 40.0 38.0 40.0 32.0 40.0 47 37.666370053983506 40.0 38.0 40.0 32.0 40.0 48 37.64413596725396 40.0 38.0 40.0 32.0 40.0 49 37.587886338019814 40.0 38.0 40.0 32.0 40.0 50 37.61415435724032 40.0 38.0 40.0 32.0 40.0 51 37.16405054280121 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.2711838195932188 2201 2 0.2794141873254361 2201 3 0.4339617814491348 2201 4 0.06230302609287719 2201 5 0.3342861602001008 2201 6 0.5426779418038237 2201 7 0.4102708294357811 2201 8 0.5798317080964566 2201 9 0.6061047859871849 2201 10 0.6007753269092291 2201 11 0.6311762391478695 2201 12 0.13213273735515685 2201 13 0.2648055398903324 2201 14 0.22418470903805598 2201 15 0.11389192290562278 2201 16 0.35400981599094195 2201 17 0.44020777958007073 2201 18 0.3286787861756224 2201 19 0.11813006434604745 2201 20 0.26722838040562635 2201 21 -0.1438383836649848 2201 22 -0.1673552924479722 2201 23 -0.4211151961219386 2201 24 -0.24553846756533204 2201 25 0.1369487909731859 2201 26 -0.03520475144551227 2201 27 -0.14383586206315613 2201 28 -0.10996254861166221 2201 29 -0.5102969811818667 2201 30 0.36719746721069413 2201 31 0.37287340578754424 2201 32 -0.22205994709535304 2201 33 -0.34953088733909254 2201 34 -1.0362130956028537 2201 35 -0.7084336551734864 2201 36 0.2589305129259074 2201 37 -0.006975276936351804 2201 38 0.4822325044049265 2201 39 0.6157427406736886 2201 40 0.3608460606309549 2201 41 0.5745049960441335 2201 42 0.019256775885352795 2201 43 0.14974281870518524 2201 44 -0.4058984305856157 2201 45 0.07798505098769226 2201 46 0.19018450480439242 2201 47 0.4474603736465923 2201 48 0.4885555806111057 2201 49 0.5853252463352518 2201 50 0.6696823082571726 2201 51 0.5129197959723371 1101 1 0.29162250354136887 1101 2 -0.2306271173320198 1101 3 0.15038394353430817 1101 4 0.3272225532257593 1101 5 0.5033503960639365 1101 6 0.3833701508908476 1101 7 0.7828673256613925 1101 8 0.03510873416112048 1101 9 0.6744711155698653 1101 10 0.25801788494939615 1101 11 0.2885612270413347 1101 12 0.48350718543947124 1101 13 0.6558467021179339 1101 14 0.3056545851240813 1101 15 0.17121993885776732 1101 16 -0.13666102861808582 1101 17 0.06632941467478304 1101 18 0.11539008086298708 1101 19 0.30115242583301693 1101 20 0.06220060658423421 1101 21 0.3434141444649086 1101 22 0.32879910151299896 1101 23 0.7663224908172452 1101 24 0.4953816292869675 1101 25 0.3706761802339713 1101 26 0.906256578849316 1101 27 0.3195596656394457 1101 28 -0.10853825007868778 1101 29 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0.36991963540648953 2202 15 0.4687127064566141 2202 16 -0.07680429904100805 2202 17 0.36671514273650274 2202 18 0.36535564085748007 2202 19 0.09451239277061063 2202 20 0.32793968910248594 2202 21 -0.23455802801063896 2202 22 -0.28891683732155116 2202 23 0.17905151685388887 2202 24 0.19444486113708592 2202 25 0.4013277851382284 2202 26 0.22000502904906938 2202 27 0.01662537716995871 2202 28 0.059528976812067924 2202 29 -0.40971348576646704 2202 30 -0.1004324299829733 2202 31 -0.2354900284997612 2202 32 0.3212854266195677 2202 33 0.23590951277205363 2202 34 -3.6980580012624387E-4 2202 35 0.35894617255643624 2202 36 0.44162014893591106 2202 37 0.3957754871647907 2202 38 0.2224381170751144 2202 39 0.3602551052193945 2202 40 0.058539864465359415 2202 41 -0.30851806591751796 2202 42 0.1704098739681541 2202 43 0.46858138497359647 2202 44 -0.19764613828220234 2202 45 -0.015652070434931886 2202 46 -0.4381844865821094 2202 47 0.15446231863131032 2202 48 -0.3796022410060118 2202 49 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1213 23 -0.6126747456612378 1213 24 -0.5995368750275105 1213 25 -0.37835094739560304 1213 26 -0.028152083944654294 1213 27 -0.6910432332382257 1213 28 0.22060280231542606 1213 29 0.010519763215818045 1213 30 -0.04316540390749424 1213 31 -0.9456341587671133 1213 32 -0.6919723575150982 1213 33 -0.36272620072778494 1213 34 -0.03695248816342911 1213 35 -0.32616770135707895 1213 36 -0.29487451494350125 1213 37 -0.1408622067509313 1213 38 -0.35760369170473183 1213 39 -0.2487777916889513 1213 40 -0.033989566715192154 1213 41 0.14901301560960434 1213 42 0.008620898283261624 1213 43 -0.15084869534327083 1213 44 -0.10621693815016897 1213 45 -0.5027793671672427 1213 46 0.06016516684510975 1213 47 0.0999458701771303 1213 48 -0.6328754283753568 1213 49 -0.5933233715694257 1213 50 -0.4464023237528352 1213 51 -0.6090231001714486 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 3.0 17 0.0 18 1.0 19 2.0 20 2.0 21 7.0 22 29.0 23 55.0 24 111.0 25 224.0 26 357.0 27 486.0 28 654.0 29 908.0 30 1197.0 31 1498.0 32 1774.0 33 2213.0 34 2660.0 35 3422.0 36 4581.0 37 7113.0 38 13658.0 39 43328.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.45503210720602 23.34627117235813 14.499875369440588 25.698821350995267 2 12.366375986237172 22.58883549860592 45.29038381681201 19.754404698344903 3 18.244052915702675 26.302426291748237 32.11959423384944 23.33392655869965 4 12.801803405113601 20.433054517411165 39.037788455834374 27.72735362164086 5 12.480275256569971 38.22981550691107 37.788455834371476 11.501453402147476 6 27.649047873287063 41.927982440529156 18.22506970398054 12.19789998220324 7 24.238002016966245 33.457910660259834 23.115619623895117 19.188467698878803 8 20.500682209171263 44.387494809278046 21.214925550216527 13.896897431334162 9 23.221213739099483 18.261849676692176 23.50477546419885 35.012161120009495 10 14.691819422198494 31.741116450139405 35.487927863795456 18.079136263866644 11 30.990093136382512 23.09189060924245 29.27923117992525 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31.02212730616361 17.05404283087145 25 18.99863558165747 32.24061220857804 30.96161831879931 17.799133890965177 26 18.99388977872694 31.259417452690275 31.74823515453521 17.998457614047577 27 18.17049297027941 31.98315239959661 30.694666903956815 19.15168772616717 28 17.975915050127544 30.29720590852465 29.22821379842202 22.49866524292579 29 20.0284748175832 28.93516046746159 28.074983686302424 22.961381028652784 30 17.73290304209577 29.92026956480471 31.346400265768114 21.000427127331402 31 21.346621581538827 29.187874473512487 30.20228984991398 19.2632140950347 32 20.63475114195883 31.14670463309011 29.241264756480987 18.97727946847007 33 18.284392240612206 30.82636293527911 31.36738446935991 19.521860354748767 34 19.42219849320757 31.48721599335588 31.545352079254908 17.545233434181647 35 20.306104289019398 30.503648336002847 30.61754760633565 18.572699768642106 36 17.89760930177374 32.03179687963457 31.041110517885745 19.029483300705937 37 18.352019932372308 30.45500385596488 28.880583733760457 22.312392477902353 38 19.281010856024203 31.141958830159577 29.89143975796405 19.685590555852166 39 20.77000652547903 28.002610191611794 29.679065076822685 21.548318206086492 40 21.978999822032392 27.639556267425995 32.36993533843508 18.011508572106543 41 20.676276917600998 26.862431037551165 31.247552945363942 21.213739099483895 42 20.465088687192264 29.98991516877262 31.012635700302543 18.532360443732575 43 17.484724446817346 29.75855727590912 33.642996974550634 19.113721302722904 44 16.91997389808388 30.66500563564098 31.362638666429376 21.05238179984576 45 17.710150086017677 29.300587293112653 32.31535860473394 20.67390401613573 46 19.922880702378833 27.021415435724034 30.646022423918843 22.40968143797829 47 20.665598861007297 27.347689387198198 29.450080085424453 22.536631666370056 48 17.64726819718811 28.20786616835736 30.024322240018986 24.120543394435543 49 20.696446580055763 27.554131814676396 30.214154357240314 21.535267248027527 50 17.72913329773981 30.60093729607878 30.717209467876845 20.95271993830456 51 16.86342441536253 28.963136100993083 31.579322045964194 22.594117437680197 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 175.0 1 90.5 2 6.0 3 13.0 4 20.0 5 18.5 6 17.0 7 17.5 8 18.0 9 17.5 10 17.0 11 43.0 12 69.0 13 102.0 14 135.0 15 164.0 16 193.0 17 162.5 18 132.0 19 126.5 20 121.0 21 127.0 22 133.0 23 123.5 24 114.0 25 161.0 26 491.5 27 775.0 28 1732.5 29 2690.0 30 4452.5 31 6215.0 32 8241.5 33 10268.0 34 11245.5 35 12223.0 36 11751.5 37 11280.0 38 9492.5 39 7705.0 40 6576.5 41 5448.0 42 4636.5 43 3825.0 44 3489.5 45 3154.0 46 3000.5 47 2847.0 48 2754.5 49 2662.0 50 2683.5 51 2705.0 52 3244.5 53 3784.0 54 2881.5 55 1979.0 56 1761.5 57 1544.0 58 1390.0 59 1236.0 60 1081.0 61 926.0 62 773.0 63 620.0 64 535.5 65 451.0 66 364.0 67 277.0 68 217.0 69 157.0 70 112.5 71 68.0 72 57.0 73 46.0 74 34.5 75 17.0 76 11.0 77 7.5 78 4.0 79 3.5 80 3.0 81 2.0 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04271222637479979 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.00474580293053331 17 0.0 18 0.003559352197899982 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0011864507326333274 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.002372901465266655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 84285.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.34037695975547 #Duplication Level Percentage of deduplicated Percentage of total 1 88.10380728198969 69.0208547406072 2 8.18259664674859 12.820554116318315 3 1.8265811822162918 4.292851750872602 4 0.7165154638926182 2.245283661553671 5 0.37167171474687977 1.4558451119274634 6 0.1989674780693481 0.9352312340810768 7 0.15622047641374862 0.8566859707759957 8 0.06773612568970126 0.424517889625966 9 0.07518074697174101 0.5300719252093967 >10 0.2779775046856365 3.8088647359560497 >50 0.01213913329658718 0.6488755674874243 >100 0.009091070946178174 1.2249791142974655 >500 0.0 0.0 >1k 0.0015151743329803692 1.73538418128736 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC 1462 1.7345909711099246 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 307 0.36424037491843153 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT 202 0.2396630479919321 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 163 0.19339146941923238 No Hit TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT 129 0.15305214450969926 RNA PCR Primer, Index 40 (95% over 21bp) GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA 129 0.15305214450969926 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 102 0.1210179747285994 No Hit GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT 95 0.11271281960016612 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC 90 0.10678056593699946 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0011864507326333274 11 0.0 0.0 0.0011864507326333274 12 0.0 0.0 0.0011864507326333274 13 0.0 0.0 0.0011864507326333274 14 0.0 0.0 0.0011864507326333274 15 0.0 0.0 0.0011864507326333274 16 0.0 0.0 0.0011864507326333274 17 0.0 0.0 0.0011864507326333274 18 0.0 0.0 0.002372901465266655 19 0.0 0.0 0.002372901465266655 20 0.0 0.0 0.002372901465266655 21 0.0 0.0 0.002372901465266655 22 0.0 0.0 0.003559352197899982 23 0.0 0.0 0.005932253663166637 24 0.0 0.0 0.013050958058966602 25 0.0 0.0 0.015423859524233256 26 0.0 0.0 0.027288366850566532 27 0.0 0.0 0.03559352197899982 28 0.0 0.0 0.0474580293053331 29 0.0 0.0 0.06525479029483301 30 0.0 0.0 0.10915346740226613 31 0.0 0.0 0.2633920626445987 32 0.0 0.0 0.4935635047754642 33 0.0 0.0 0.735599454232663 34 0.0 0.0 1.0440766447173282 35 0.0 0.0 1.3395028771430266 36 0.0 0.0 1.739336774040458 37 0.0 0.0 2.2127306163611555 38 0.0 0.0 2.852227561250519 39 0.0 0.0 3.5308773803167823 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 140 0.0 38.571426 45 GCCCACG 150 0.0 37.500004 18 CTCCGAG 150 0.0 37.500004 12 CCCACGA 150 0.0 37.500004 19 CGAGCCC 150 0.0 37.500004 15 TCTCCGA 150 0.0 37.500004 11 CCACGAG 150 0.0 37.500004 20 GACCTTG 150 0.0 37.500004 26 CATACAC 30 1.13096714E-4 37.499996 5 CTCGTAT 140 0.0 36.964283 39 TCTCGTA 140 0.0 36.964283 38 ATCTCCG 155 0.0 36.29032 10 TCCGAGC 155 0.0 36.29032 13 CACGAGA 155 0.0 36.29032 21 CCGAGCC 155 0.0 36.29032 14 CGAGACC 155 0.0 36.29032 23 ACGAGAC 155 0.0 36.29032 22 ACCTTGC 155 0.0 36.29032 27 GTCTTAA 25 0.002096068 36.0 1 GCCGTCT 25 0.002096068 36.0 45 >>END_MODULE