FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_27.341000000069ce.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_27.341000000069ce.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84285
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC14621.7345909711099246No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA3070.36424037491843153No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT2020.2396630479919321No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1630.19339146941923238No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT1290.15305214450969926RNA PCR Primer, Index 40 (95% over 21bp)
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA1290.15305214450969926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.1210179747285994No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT950.11271281960016612No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC900.10678056593699946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1400.038.57142645
GCCCACG1500.037.50000418
CTCCGAG1500.037.50000412
CCCACGA1500.037.50000419
CGAGCCC1500.037.50000415
TCTCCGA1500.037.50000411
CCACGAG1500.037.50000420
GACCTTG1500.037.50000426
CATACAC301.13096714E-437.4999965
CTCGTAT1400.036.96428339
TCTCGTA1400.036.96428338
ATCTCCG1550.036.2903210
TCCGAGC1550.036.2903213
CACGAGA1550.036.2903221
CCGAGCC1550.036.2903214
CGAGACC1550.036.2903223
ACGAGAC1550.036.2903222
ACCTTGC1550.036.2903227
GTCTTAA250.00209606836.01
GCCGTCT250.00209606836.045