Basic Statistics
Measure | Value |
---|---|
Filename | H23NVBCXX l02n01 48h_27.341000000069ce.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 84285 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 40 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC | 1462 | 1.7345909711099246 | No Hit |
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA | 307 | 0.36424037491843153 | No Hit |
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT | 202 | 0.2396630479919321 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 163 | 0.19339146941923238 | No Hit |
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT | 129 | 0.15305214450969926 | RNA PCR Primer, Index 40 (95% over 21bp) |
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA | 129 | 0.15305214450969926 | No Hit |
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 102 | 0.1210179747285994 | No Hit |
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT | 95 | 0.11271281960016612 | No Hit |
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC | 90 | 0.10678056593699946 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCCGTC | 140 | 0.0 | 38.571426 | 45 |
GCCCACG | 150 | 0.0 | 37.500004 | 18 |
CTCCGAG | 150 | 0.0 | 37.500004 | 12 |
CCCACGA | 150 | 0.0 | 37.500004 | 19 |
CGAGCCC | 150 | 0.0 | 37.500004 | 15 |
TCTCCGA | 150 | 0.0 | 37.500004 | 11 |
CCACGAG | 150 | 0.0 | 37.500004 | 20 |
GACCTTG | 150 | 0.0 | 37.500004 | 26 |
CATACAC | 30 | 1.13096714E-4 | 37.499996 | 5 |
CTCGTAT | 140 | 0.0 | 36.964283 | 39 |
TCTCGTA | 140 | 0.0 | 36.964283 | 38 |
ATCTCCG | 155 | 0.0 | 36.29032 | 10 |
TCCGAGC | 155 | 0.0 | 36.29032 | 13 |
CACGAGA | 155 | 0.0 | 36.29032 | 21 |
CCGAGCC | 155 | 0.0 | 36.29032 | 14 |
CGAGACC | 155 | 0.0 | 36.29032 | 23 |
ACGAGAC | 155 | 0.0 | 36.29032 | 22 |
ACCTTGC | 155 | 0.0 | 36.29032 | 27 |
GTCTTAA | 25 | 0.002096068 | 36.0 | 1 |
GCCGTCT | 25 | 0.002096068 | 36.0 | 45 |