FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_26.341000000069b1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_26.341000000069b1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60892
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14112.3172173684556263No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9191.5092294554292847No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC8991.4763844183143926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8211.348288773566314No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6371.0461144321093083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6341.0411876765420744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6341.0411876765420744No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3420.5616501346646522No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3370.5534388753859292No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.4959600604348683No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2880.47296853445444387No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.47132628259869935No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2580.423700978782106No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA2270.3727911712540235No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.3629376601195559No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.35144189712934376No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1960.321881363725941No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA1740.28575182289955986No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1610.2644025487748801No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT1460.23976877093871116RNA PCR Primer, Index 40 (95% over 21bp)
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.23976877093871116No Hit
GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.2299152598042436No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1360.2233462523812652No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA1310.2151349931025422No Hit
GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1300.2134927412467976No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1280.2102082375353084No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1130.18557445969913947No Hit
GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1100.18064770413190567No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.1543716744399921No Hit
CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.13630690402680154No Hit
GCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA720.11824213361361097No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA690.1133153780463772No Hit
GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.1116731261906326No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA650.1067463706233988No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT250.002091685535.99934445
TGTCCAC250.002091685535.99934410
GCCCACG1050.034.28508818
AGCCCAC1050.034.28508817
CACGAGA1050.034.28508821
CCCACGA1050.034.28508819
CGAGCCC1050.034.28508815
CCACGAG1050.034.28508820
ACCTTGC1050.034.28508827
ATCTCGT1000.033.74938637
CTCGTAT1000.033.74938639
TCTCGTA1000.033.74938638
AATCTCG1000.033.74938636
TGCCGTC1000.033.74938645
GAGCCCA1100.032.72667716
GTATGCC1050.032.1422742
ATGCCGT1050.032.1422744
CTCCGAG1150.031.30377612
GAGACCT1150.031.30377624
AGACCTT1150.031.30377625
Produced by FastQC (version 0.11.2)