##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_25.341000000069a5.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 18947 Sequences flagged as poor quality 0 Sequence length 51 %GC 23 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.37562674829788 38.0 38.0 38.0 32.0 38.0 2 36.26679685438328 38.0 38.0 38.0 32.0 38.0 3 36.377949015675306 38.0 38.0 38.0 32.0 38.0 4 36.356204148413994 38.0 38.0 38.0 32.0 38.0 5 36.345067820763184 38.0 38.0 38.0 32.0 38.0 6 38.31250329867525 40.0 38.0 40.0 38.0 40.0 7 38.18630917823402 40.0 38.0 40.0 38.0 40.0 8 38.16567266585739 40.0 38.0 40.0 38.0 40.0 9 38.182720219559826 40.0 38.0 40.0 38.0 40.0 10 38.08581833535652 40.0 38.0 40.0 38.0 40.0 11 38.01651976566211 40.0 38.0 40.0 32.0 40.0 12 37.91259830052251 40.0 38.0 40.0 32.0 40.0 13 37.881458806143456 40.0 38.0 40.0 32.0 40.0 14 37.74212276349818 40.0 38.0 40.0 32.0 40.0 15 37.714519448989286 40.0 38.0 40.0 32.0 40.0 16 37.750092362907054 40.0 38.0 40.0 32.0 40.0 17 37.84488309494907 40.0 38.0 40.0 32.0 40.0 18 37.85221934870956 40.0 38.0 40.0 32.0 40.0 19 37.99356098590806 40.0 38.0 40.0 32.0 40.0 20 38.01229746133953 40.0 38.0 40.0 32.0 40.0 21 38.11864675146461 40.0 38.0 40.0 38.0 40.0 22 38.07315142238877 40.0 38.0 40.0 32.0 40.0 23 38.04407030136697 40.0 38.0 40.0 32.0 40.0 24 38.061434527893596 40.0 38.0 40.0 32.0 40.0 25 38.00796959940888 40.0 38.0 40.0 32.0 40.0 26 37.959149205679 40.0 38.0 40.0 32.0 40.0 27 37.90225365493218 40.0 38.0 40.0 32.0 40.0 28 37.79585158600306 40.0 38.0 40.0 32.0 40.0 29 37.819390932601465 40.0 38.0 40.0 32.0 40.0 30 37.725497440228004 40.0 38.0 40.0 32.0 40.0 31 37.7240196337151 40.0 38.0 40.0 32.0 40.0 32 37.671293608486835 40.0 38.0 40.0 32.0 40.0 33 37.588061434527894 40.0 38.0 40.0 32.0 40.0 34 37.59170317200612 40.0 38.0 40.0 32.0 40.0 35 37.60785348604001 40.0 38.0 40.0 32.0 40.0 36 37.449147622314875 40.0 38.0 40.0 32.0 40.0 37 37.41795534913179 40.0 38.0 40.0 32.0 40.0 38 37.300416952551856 40.0 38.0 40.0 32.0 40.0 39 37.177864569588856 40.0 38.0 40.0 32.0 40.0 40 37.13469150789043 40.0 38.0 40.0 32.0 40.0 41 37.00585844724758 40.0 38.0 40.0 32.0 40.0 42 36.82920779015148 40.0 38.0 40.0 32.0 40.0 43 36.792368184936926 40.0 38.0 40.0 27.0 40.0 44 36.57681954926901 40.0 38.0 40.0 27.0 40.0 45 36.45284213859714 40.0 38.0 40.0 27.0 40.0 46 36.228532221459865 40.0 38.0 40.0 27.0 40.0 47 36.13764712091624 40.0 38.0 40.0 27.0 40.0 48 35.95339631603948 40.0 38.0 40.0 27.0 40.0 49 35.815854752731305 40.0 38.0 40.0 27.0 40.0 50 35.601836702380325 40.0 38.0 40.0 13.0 40.0 51 35.54731619781496 40.0 38.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 -0.5624600927704719 2201 2 -0.6246127293733323 2201 3 -2.376771938867819 2201 4 -0.9621282179425563 2201 5 0.36530480857928893 2201 6 0.22259444982932308 2201 7 0.38488612842815684 2201 8 0.07073351164375907 2201 9 -0.3515128855981402 2201 10 0.48473442658735166 2201 11 0.2834266087700854 2201 12 0.35722730678918424 2201 13 0.20804728256362637 2201 14 -1.1001457876510585 2201 15 -0.24030212694184172 2201 16 1.1252296964151682 2201 17 0.1006226203350522 2201 18 0.74509781581461 2201 19 0.876506302371844 2201 20 0.898370708061293 2201 21 0.5239001151726868 2201 22 0.25977940756858686 2201 23 1.2340487135818492 2201 24 0.22405498970607596 2201 25 0.8591760859086222 2201 26 0.45654171988153536 2201 27 -0.10929474107147996 2201 28 0.20669344196981854 2201 29 -0.5135484222777507 2201 30 -1.2650893828573047 2201 31 -1.6091648764716666 2201 32 -0.9541931068329319 2201 33 -0.7572440554787363 2201 34 -1.027686218244476 2201 35 -1.0204972523302516 2201 36 -0.1832668680697509 2201 37 0.695688760499074 2201 38 -0.23140077210378962 2201 39 0.5546836235763166 2201 40 -0.6247454460280935 2201 41 -3.0533163262594627 2201 42 -0.8904958409453982 2201 43 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0.11668676652889332 1209 44 0.8828890866673973 1209 45 -0.09865946900724509 1209 46 0.6807699476647926 1209 47 0.8316847288763185 1209 48 0.664374697821394 1209 49 0.03879373989985169 1209 50 1.9864786850417957 1209 51 0.8197710621634258 1214 1 -0.8957934261038076 1214 2 -1.114196062706668 1214 3 -0.6580219388678188 1214 4 -1.5662948846092206 1214 5 -1.0826118580873754 1214 6 -0.2565722168373412 1214 7 0.06196946176149254 1214 8 0.060316844977094775 1214 9 -1.1327628855981402 1214 10 -1.036098906745984 1214 11 -1.4665733912299146 1214 12 -0.6531893598774801 1214 13 -2.3752860507697093 1214 14 -3.4647291209843942 1214 15 -1.031968793608506 1214 16 0.25022969641516823 1214 17 -0.4410440463316121 1214 18 0.3805144824812743 1214 19 -0.24849369762815599 1214 20 -0.46621262527204266 1214 21 0.7009834485060225 1214 22 0.10352940756858686 1214 23 -0.2867846197514865 1214 24 -0.19261167696058834 1214 25 0.015426085908622156 1214 26 0.25862505321487106 1214 27 -0.04679474107147996 1214 28 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1215 14 -0.5918124543177257 1215 15 -0.4486354602751703 1215 16 -0.17477030358482892 1215 17 0.5589559536683879 1215 18 0.011764482481275707 1215 19 0.943172969038514 1215 20 -0.7932959586053769 1215 21 -0.0010998848273118256 1215 22 0.11811274090192114 1215 23 -0.4992846197514851 1215 24 -1.592611676960587 1215 25 -0.5741572474247079 1215 26 -0.518458280118459 1215 27 0.28237192559518576 1215 28 0.24002677530315708 1215 29 -1.1635484222777492 1215 30 0.6682439504760254 1215 31 0.6908351235283376 1215 32 1.0624735598337338 1215 33 1.1760892778545937 1215 34 1.3223137817555184 1215 35 1.2461694143364141 1215 36 1.5000664652635791 1215 37 1.7456887604990712 1215 38 -0.5147341054371211 1215 39 0.7796836235763109 1215 40 1.6502545539719051 1215 41 0.8716836737405416 1215 42 0.9511708257212632 1215 43 0.056270099862224754 1215 44 1.1203890866673945 1215 45 0.4159238643260892 1215 46 0.44951994766478975 1215 47 -0.053731937790345796 1215 48 -1.4210419688452731 1215 49 0.1700437398998531 1215 50 -0.6010213149582029 1215 51 0.11768772883009149 1212 1 -0.5779362832466646 1212 2 -1.247231776992379 1212 3 -0.3767719388678188 1212 4 -1.046652027466365 1212 5 -0.9977904295159448 1212 6 -1.0333579311230565 1212 7 0.6039337474757787 1212 8 -0.449504583594333 1212 9 -1.2265128855981402 1212 10 -0.9843131924602702 1212 11 -1.2058591055156285 1212 12 -0.5630107884489064 1212 13 -0.45742890791256485 1212 14 -0.47276483527010527 1212 15 0.1037454921057801 1212 16 -1.4461988750134012 1212 17 -0.026758332045901057 1212 18 0.11176448248127713 1212 19 -0.38063655477101577 1212 20 0.282894517585099 1212 21 0.04651916279173918 1212 22 -0.5723634495742687 1212 23 0.3292868088199441 1212 24 -0.36404024838915916 1212 25 -0.5408239140913764 1212 26 -0.5994106610708414 1212 27 -0.07000902678576892 1212 28 0.268598203874582 1212 29 0.5554991967698726 1212 30 -0.22223224000016017 1212 31 -0.23297440028118643 1212 32 0.1529497503099222 1212 33 1.0998988016641178 1212 34 1.1032661627079037 1212 35 1.0271217952887994 1212 36 -0.5189811537840399 1212 37 -1.7305017156914033 1212 38 -0.1194960101990219 1212 39 -1.8965068526141593 1212 40 -2.368793065075714 1212 41 -1.152125850068984 1212 42 -0.5059720314215923 1212 43 0.08007962367175026 1212 44 -0.9938966276183194 1212 45 -0.5031237547215284 1212 46 -1.2076229094780615 1212 47 -1.7013509854093982 1212 48 -0.45437530217860456 1212 49 -0.9013848315287163 1212 50 -1.2153070292439168 1212 51 0.2653067764491368 1213 1 0.31253990722952807 1213 2 -0.8607238404844466 1213 3 0.4565613944655169 1213 4 -0.5037948846092206 1213 5 -0.3985840803095968 1213 6 -0.23573888350400551 1213 7 0.1626639062059354 1213 8 -1.6375998216895695 1213 9 -1.2542906633759188 1213 10 -0.26526557341264834 1213 11 -1.5360178356743575 1213 12 -0.4899949154330372 1213 13 -1.0002860507697093 1213 14 0.46929865679338434 1213 15 0.13469787305815828 1213 16 -0.8747703035848318 1213 17 0.1422892870017236 1213 18 0.5228755935923886 1213 19 -0.6512714754059346 1213 20 0.4678151525057359 1213 21 0.14890011517268675 1213 22 0.6347794075685869 1213 23 0.03960426913739923 1213 24 -0.35927834362725264 1213 25 0.22028719701973642 1213 26 0.05376394210375679 1213 27 -0.6509614077381443 1213 28 -0.7933065580301815 1213 29 -0.04132620005552923 1213 30 0.48491061714269534 1213 31 0.33527956797278335 1213 32 1.045806893167068 1213 33 0.659422611187928 1213 34 1.0000915595333026 1213 35 0.31283608100308413 1213 36 0.7333997985969134 1213 37 0.8623554271657383 1213 38 1.2685992278962104 1213 39 0.09635029024298092 1213 40 1.2502545539719065 1213 41 0.12723922929609444 1213 42 0.6233930479434875 1213 43 1.2729367665288933 1213 44 1.459277975556283 1213 45 0.48814608654831204 1213 46 0.4828532809981283 1213 47 0.8351569510985399 1213 48 2.0289580311547297 1213 49 1.486710406566516 1213 50 1.7989786850417957 1213 51 1.4899099510523115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 3.0 16 0.0 17 1.0 18 3.0 19 9.0 20 8.0 21 14.0 22 20.0 23 44.0 24 55.0 25 74.0 26 116.0 27 172.0 28 214.0 29 260.0 30 294.0 31 400.0 32 497.0 33 616.0 34 718.0 35 921.0 36 1155.0 37 1540.0 38 2720.0 39 9093.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.53892847717076 27.59567168118237 14.536817102137768 15.328582739509105 2 9.637409616298093 29.835857919459546 35.51485723333509 25.011875230907265 3 21.961260357840292 37.13516651712672 30.073362537604897 10.83021058742809 4 10.97799123871853 26.5794057106666 34.73900881406028 27.7035942365546 5 10.006861244524199 37.19850108196548 33.98427191639837 18.810365757111942 6 29.086398902200877 41.33108143769462 11.463556235815696 18.118963424288808 7 10.444925317992295 46.0495065181823 20.625956615823085 22.87961154800232 8 19.760384229693358 34.87095582414103 23.956299150261255 21.412360795904366 9 18.351190162030928 29.334459281152693 22.57877236501821 29.735578191798172 10 12.878028183881355 35.145405605108984 25.629387238085183 26.347178972924475 11 19.644270860822292 31.577558452525466 21.69736633767879 27.08080434897345 12 18.900089723966854 37.984905262046766 28.183881353248534 14.931123660737846 13 23.4021217079221 29.001952815749192 28.02554494115163 19.570380535177073 14 18.045073098643584 31.382276877605953 32.18451469889692 18.38813532485354 15 22.346545627276086 30.99699160817016 29.946693407927377 16.709769356626378 16 12.186625851058215 40.73995883253285 26.901356415263628 20.172058901145302 17 11.774951179606271 40.11189106454848 32.74924790204254 15.363909853802712 18 11.84356362484826 31.815063070670817 42.76138702696997 13.579986277510953 19 11.363276508154325 38.78714308333773 37.668232437852964 12.181347970654985 20 11.115216129202512 32.69646909801024 39.46271177495118 16.72560299783607 21 5.715944476698158 40.63967910487148 39.341320525676885 14.30305589275347 22 6.9245790890378425 43.732517021164306 39.66327123027392 9.679632659523934 23 6.212065234601784 38.78714308333773 41.71636670713041 13.284424974930067 24 9.653243257507786 41.79553491317887 42.00665012930807 6.544571700005278 25 8.676835382910223 41.91164828204993 40.28078323745184 9.13073309758801 26 8.376438298321546 40.97962630634434 42.50501425102924 8.138921144304867 27 7.726816910328813 43.5741806090674 42.25471050825988 6.444291972343906 28 5.510107140972186 40.76634823454901 42.344434475114795 11.379110149364015 29 6.344012244682536 41.336359318097855 43.16250593761545 9.157122499604158 30 6.428458331134217 40.63967910487148 45.87005858447248 7.061803979521825 31 7.151527946376735 41.85886947801763 44.065023486567796 6.9245790890378425 32 9.531852008233493 42.502770887211696 40.771626114952234 7.193750989602575 33 5.86900300839183 44.08613500818072 40.72412519132316 9.320736792104292 34 6.159286430569484 47.26341901092521 40.660790626484406 5.9165039320209 35 8.529054731619782 44.967541035520135 40.29661687866153 6.206787354198553 36 5.3623264896817435 48.11843563624848 40.571066659629494 5.948171214440281 37 5.9323375732305905 47.30036417374782 40.49717633398427 6.270121919037315 38 5.795112682746609 44.6455903309231 42.70860822293766 6.850688763392622 39 6.090673985327492 44.35002902834222 40.4496754103552 9.109621575975089 40 5.969282736053201 44.37641843035836 43.004169525518556 6.65012930806988 41 6.639573547263419 44.47669815801974 39.58410302422547 9.29962527049137 42 9.062120652346017 44.70892489576187 39.80577400116113 6.423180450730986 43 6.386235287908376 44.86726130785876 42.05415105293714 6.69235235129572 44 6.159286430569484 47.6117591175384 39.10381590753153 7.125138544360585 45 6.850688763392622 44.62975668971341 41.23607959043648 7.2834749564574865 46 9.647965377104555 44.38697419116483 38.14324167414366 7.821818757586953 47 6.613184145247268 44.51892120124558 38.053517707288755 10.814376946218399 48 7.151527946376735 44.40808571277775 37.64712091624004 10.79326542460548 49 9.901303636459598 44.19169261624531 38.08518498970813 7.821818757586953 50 7.214862511215496 44.175858975035624 40.148836227371085 8.460442286377791 51 7.294030717263947 44.00696680213226 37.59961999261097 11.099382487992822 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1452.0 1 798.5 2 145.0 3 255.5 4 366.0 5 342.0 6 318.0 7 431.5 8 545.0 9 463.5 10 382.0 11 575.5 12 769.0 13 1219.0 14 1669.0 15 2000.5 16 2332.0 17 1719.0 18 1106.0 19 914.5 20 723.0 21 966.0 22 1209.0 23 946.5 24 684.0 25 612.0 26 794.0 27 1048.0 28 754.0 29 460.0 30 349.0 31 238.0 32 253.5 33 269.0 34 296.5 35 324.0 36 326.0 37 328.0 38 356.0 39 384.0 40 349.5 41 315.0 42 310.0 43 305.0 44 325.5 45 346.0 46 336.5 47 327.0 48 329.5 49 332.0 50 369.0 51 406.0 52 609.0 53 812.0 54 519.5 55 227.0 56 198.5 57 170.0 58 152.5 59 135.0 60 123.0 61 111.0 62 88.0 63 65.0 64 57.5 65 50.0 66 38.0 67 26.0 68 21.0 69 16.0 70 12.5 71 9.0 72 6.0 73 3.0 74 2.0 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010555760806460125 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.005277880403230063 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 18947.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.42544993930437 #Duplication Level Percentage of deduplicated Percentage of total 1 93.63982513044704 35.04512587744762 2 2.9756028768861937 2.2272655301630864 3 0.7051191651389085 0.7916820604845094 4 0.43717388238612326 0.6544571700005277 5 0.1833309829361162 0.3430622262099541 6 0.1833309829361162 0.4116746714519449 7 0.11281906642222536 0.29556130258088353 8 0.09871668311944717 0.29556130258088353 9 0.04230714990833451 0.1425027708872117 >10 1.0294739811028064 8.502665329603632 >50 0.23974051614722885 6.291233440650235 >100 0.26794528275278523 19.950387924209636 >500 0.05640953321111268 11.611336887106138 >1k 0.02820476660555634 13.43748350662374 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1360 7.177917348392886 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1186 6.259566158230855 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 625 3.298675252018789 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 526 2.776165092099013 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 526 2.776165092099013 No Hit CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC 523 2.760331450889323 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 447 2.359212540243838 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 360 1.9000369451628227 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 284 1.4989180345173378 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 273 1.4408613500818073 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 258 1.3616931440333562 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 234 1.2350240143558349 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 231 1.2191903731461446 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 195 1.0291866786298622 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 187 0.9869636354040217 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 179 0.9447405921781813 No Hit GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 150 0.7916820604845094 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 141 0.7441811368554389 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 140 0.7389032564522088 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 137 0.7230696152425187 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 123 0.6491792895972976 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 115 0.6069562463714573 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 110 0.5805668443553069 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 109 0.5752889639520768 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 107 0.5647332031456167 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 94 0.49612075790362586 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 86 0.45389771467778545 No Hit GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 83 0.4380640734680952 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 82 0.43278619306486515 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 81 0.4275083126616351 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 76 0.40111891064548477 No Hit TTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCT 74 0.3905631498390246 RNA PCR Primer, Index 40 (95% over 21bp) AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 72 0.38000738903256454 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.3641737478228743 No Hit CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 64 0.337784345806724 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA 63 0.33250646540349393 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 62 0.32722858500026386 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA 60 0.31667282419380377 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA 60 0.31667282419380377 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 56 0.29556130258088353 No Hit GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 56 0.29556130258088353 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 54 0.2850055417744234 No Hit GCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 48 0.253338259355043 No Hit ATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 43 0.22694885733889272 No Hit TTTCCAAGCAGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 43 0.22694885733889272 No Hit GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42 0.22167097693566265 No Hit TACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40 0.2111152161292025 No Hit GATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 39 0.20583733572597246 No Hit GCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 38 0.20055945532274239 No Hit CTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 38 0.20055945532274239 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAA 35 0.1847258141130522 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34 0.17944793370982215 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 34 0.17944793370982215 No Hit TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33 0.17417005330659208 No Hit CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33 0.17417005330659208 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA 32 0.168892172903362 No Hit TGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 31 0.16361429250013193 No Hit ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 30 0.15833641209690189 No Hit AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 29 0.15305853169367184 No Hit GTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAA 27 0.1425027708872117 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAA 27 0.1425027708872117 No Hit CTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 27 0.1425027708872117 No Hit ATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 27 0.1425027708872117 No Hit ACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAA 26 0.13722489048398162 No Hit ATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCTTC 26 0.13722489048398162 RNA PCR Primer, Index 40 (100% over 22bp) TTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26 0.13722489048398162 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25 0.13194701008075158 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25 0.13194701008075158 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAA 24 0.1266691296775215 No Hit CCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 24 0.1266691296775215 No Hit GCGTTGATACCACTGCTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.12139124927429146 No Hit ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA 22 0.11611336887106138 No Hit CTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22 0.11611336887106138 No Hit CCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22 0.11611336887106138 No Hit TACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22 0.11611336887106138 No Hit TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 21 0.11083548846783133 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAA 21 0.11083548846783133 No Hit TTTTTGCCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21 0.11083548846783133 No Hit TATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTCTT 21 0.11083548846783133 RNA PCR Primer, Index 40 (100% over 21bp) AAAGTACTCTGCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAA 20 0.10555760806460125 No Hit GTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAA 20 0.10555760806460125 No Hit GCTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.10027972766137119 No Hit GTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.10027972766137119 No Hit CTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.10027972766137119 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.005277880403230063 10 0.0 0.0 0.005277880403230063 11 0.0 0.0 0.005277880403230063 12 0.0 0.0 0.005277880403230063 13 0.0 0.0 0.005277880403230063 14 0.0 0.0 0.005277880403230063 15 0.0 0.0 0.005277880403230063 16 0.0 0.0 0.005277880403230063 17 0.0 0.0 0.005277880403230063 18 0.0 0.0 0.005277880403230063 19 0.0 0.0 0.005277880403230063 20 0.0 0.0 0.005277880403230063 21 0.0 0.0 0.010555760806460125 22 0.0 0.0 0.010555760806460125 23 0.0 0.0 0.010555760806460125 24 0.0 0.0 0.02111152161292025 25 0.0 0.0 0.031667282419380376 26 0.0 0.0 0.06333456483876075 27 0.0 0.0 0.06861244524199081 28 0.0 0.0 0.11611336887106138 29 0.0 0.0 0.1530585316936718 30 0.0 0.0 0.21639309653243258 31 0.0 0.0 0.33250646540349393 32 0.0 0.0 0.422230432258405 33 0.0 0.0 0.5436216815326965 34 0.0 0.0 0.6861244524199082 35 0.0 0.0 0.7811262996780493 36 0.0 0.0 0.9552963529846413 37 0.0 0.0 1.092521243468623 38 0.0 0.0 1.266691296775215 39 0.0 0.0 1.4830843933076476 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCTCG 70 1.8189894E-11 35.357143 36 CCTTGCA 70 1.8189894E-11 35.357143 28 AGAATCT 70 1.8189894E-11 35.357143 34 GAATCTC 70 1.8189894E-11 35.357143 35 GCAGAAT 70 1.8189894E-11 35.357143 32 ACCTTGC 70 1.8189894E-11 35.357143 27 CTTGCAG 70 1.8189894E-11 35.357143 29 AGACCTT 70 1.8189894E-11 35.357143 25 CAGAATC 70 1.8189894E-11 35.357143 33 GACCTTG 70 1.8189894E-11 35.357143 26 CGAGACC 70 1.8189894E-11 35.357143 23 GAGACCT 70 1.8189894E-11 35.357143 24 AGCCCAC 80 1.8189894E-12 33.75 17 GTATGCC 80 1.8189894E-12 33.75 42 ATCTCGT 80 1.8189894E-12 33.75 37 CCACGAG 80 1.8189894E-12 33.75 20 TATGCCG 80 1.8189894E-12 33.75 43 GAGCCCA 80 1.8189894E-12 33.75 16 CACGAGA 80 1.8189894E-12 33.75 21 TCTCGTA 80 1.8189894E-12 33.75 38 >>END_MODULE