FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_24.34100000006998.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_24.34100000006998.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2307
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC112348.67793671434764No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT833.5977459904638054RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC652.8175119202427394No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA341.4737754659731253No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.8669267446900737No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC180.7802340702210664No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT170.7368877329865626No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC160.693541395752059No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.5635023840485479No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC130.5635023840485479No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.5201560468140443No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC110.4768097095795405RNA PCR Primer, Index 27 (95% over 21bp)
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.43346337234503685No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACT100.43346337234503685No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.43346337234503685No Hit
GCGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGA90.3901170351105332No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC80.3467706978760295TruSeq Adapter, Index 12 (95% over 21bp)
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.3467706978760295No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.3467706978760295No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA70.30342436064152584No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.26007802340702213No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.26007802340702213No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC60.26007802340702213No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.21673168617251842No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT50.21673168617251842RNA PCR Primer, Index 38 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC50.21673168617251842No Hit
TTTTTCAAGTCGTCAAGTGGATGTTTCTCATTTTCCATGATTTTCAGTTTT50.21673168617251842No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.21673168617251842No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.17338534893801474No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG40.17338534893801474No Hit
CTTATACACATCTACGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC40.17338534893801474No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.17338534893801474No Hit
TATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCTT30.13003901170351106RNA PCR Primer, Index 27 (100% over 21bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAACTGTCTCT30.13003901170351106No Hit
ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA30.13003901170351106No Hit
ACTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT30.13003901170351106No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.13003901170351106No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.13003901170351106No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA30.13003901170351106No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.13003901170351106No Hit
CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13003901170351106No Hit
CGGAGATGTGTATAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACT30.13003901170351106No Hit
GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13003901170351106No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGAGCCTATCTCGTATGCCGTC30.13003901170351106No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATATCGTATGCCGTC30.13003901170351106No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA30.13003901170351106No Hit
CGGAGATGTGTATAAGAGACAGGCGTCAGATGTGTATAAGAGACAGCTTAT30.13003901170351106No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA30.13003901170351106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT205.664452E-445.00000445
CTTATAC1450.041.896551
AGCCCAC1550.039.19354617
ATCTCCG1550.039.19354610
GCCCACG1550.039.19354618
CCACGAG1550.039.19354620
GAGCCCA1550.039.19354616
CACGAGA1550.039.19354621
CCCACGA1550.039.19354619
ACGAGAC1550.039.19354622
CGAGCCC1550.039.19354615
CCGAGCC1550.039.19354614
TCTCCGA1550.039.19354611
TCCGAGC1550.039.19354613
CTCCGAG1550.039.19354612
TGCCGTC1500.039.045
CCTATCT1400.038.57142634
TATCTCG1400.038.57142636
CGAGACT1400.038.57142623
AGACTAG1400.038.57142625
Produced by FastQC (version 0.11.2)