##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_23.3410000000698b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24397 Sequences flagged as poor quality 0 Sequence length 51 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.41451817846457 38.0 38.0 38.0 32.0 38.0 2 36.50645571176784 38.0 38.0 38.0 32.0 38.0 3 36.475714227159074 38.0 38.0 38.0 32.0 38.0 4 36.35824076730746 38.0 38.0 38.0 32.0 38.0 5 36.42845431815387 38.0 38.0 38.0 32.0 38.0 6 38.33479526171251 40.0 38.0 40.0 38.0 40.0 7 38.148255933106526 40.0 38.0 40.0 38.0 40.0 8 38.16875025617904 40.0 38.0 40.0 38.0 40.0 9 38.21687092675329 40.0 38.0 40.0 38.0 40.0 10 38.09107677173423 40.0 38.0 40.0 38.0 40.0 11 38.06869697093905 40.0 38.0 40.0 32.0 40.0 12 37.94630487355003 40.0 38.0 40.0 32.0 40.0 13 37.94970693118006 40.0 38.0 40.0 32.0 40.0 14 37.86465549042915 40.0 38.0 40.0 32.0 40.0 15 37.87572242488831 40.0 38.0 40.0 32.0 40.0 16 37.84108701889576 40.0 38.0 40.0 32.0 40.0 17 37.83739804074271 40.0 38.0 40.0 32.0 40.0 18 37.715128909292126 40.0 38.0 40.0 32.0 40.0 19 37.86563921793663 40.0 38.0 40.0 32.0 40.0 20 37.86969709390499 40.0 38.0 40.0 32.0 40.0 21 37.89433127023814 40.0 38.0 40.0 32.0 40.0 22 37.89506906586875 40.0 38.0 40.0 32.0 40.0 23 37.76378243226626 40.0 38.0 40.0 32.0 40.0 24 37.774234537033244 40.0 38.0 40.0 32.0 40.0 25 37.69508546132721 40.0 38.0 40.0 32.0 40.0 26 37.5742509324917 40.0 38.0 40.0 32.0 40.0 27 37.45731032503996 40.0 38.0 40.0 32.0 40.0 28 37.43702094519818 40.0 38.0 40.0 32.0 40.0 29 37.40185268680575 40.0 38.0 40.0 32.0 40.0 30 37.35229741361643 40.0 38.0 40.0 32.0 40.0 31 37.267409927450096 40.0 38.0 40.0 32.0 40.0 32 37.156699594212405 40.0 38.0 40.0 32.0 40.0 33 37.08365782678198 40.0 38.0 40.0 32.0 40.0 34 37.07017256220027 40.0 38.0 40.0 32.0 40.0 35 37.054268967496 40.0 38.0 40.0 32.0 40.0 36 36.93823011025946 40.0 38.0 40.0 32.0 40.0 37 36.813706603270894 40.0 38.0 40.0 32.0 40.0 38 36.84383325818748 40.0 38.0 40.0 32.0 40.0 39 36.69233922203549 40.0 38.0 40.0 32.0 40.0 40 36.677296388900274 40.0 38.0 40.0 32.0 40.0 41 36.70385703160225 40.0 38.0 40.0 32.0 40.0 42 36.683157765299015 40.0 38.0 40.0 32.0 40.0 43 36.59904906340944 40.0 38.0 40.0 27.0 40.0 44 36.557732508095256 40.0 38.0 40.0 27.0 40.0 45 36.487805877771855 38.0 38.0 40.0 27.0 40.0 46 36.374390293888595 38.0 38.0 40.0 27.0 40.0 47 36.32745829405255 38.0 38.0 40.0 27.0 40.0 48 36.22781489527401 38.0 38.0 40.0 27.0 40.0 49 36.14903471738329 38.0 38.0 40.0 27.0 40.0 50 36.11177603803746 38.0 38.0 40.0 27.0 40.0 51 35.306759027749315 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.9528780798471459 2201 2 0.8917414406281381 2201 3 0.5831496939623975 2201 4 -0.0649330590158641 2201 5 0.5283182744903812 2201 6 0.972558536728755 2201 7 0.9207573056311986 2201 8 1.3133739711955243 2201 9 0.8525386183455623 2201 10 0.6040954868437538 2201 11 0.5420715018672695 2201 12 1.2816673612546978 2201 13 0.48417086591631886 2201 14 1.4755158879007482 2201 15 1.4901465591802818 2201 16 1.0940535336329091 2201 17 1.187156149142666 2201 18 0.81394457081403 2201 19 0.7861445669873177 2201 20 1.1995393534788406 2201 21 0.9792337819500219 2201 22 0.900500857923106 2201 23 0.4499446790302386 2201 24 0.7331987273839857 2201 25 -0.24213376541851517 2201 26 1.096479306091119 2201 27 0.2559309280474764 2201 28 0.4927669576557463 2201 29 1.0994515877770255 2201 30 1.5366509660370085 2201 31 0.11144875023661172 2201 32 -0.40969371992538584 2201 33 1.311036915054089 2201 34 -0.5218745225765247 2201 35 0.4843686009688781 2201 36 0.16943387974439617 2201 37 -0.49093213074454667 2201 38 0.5786532622133223 2201 39 0.07020706151423894 2201 40 0.43844316096509317 2201 41 0.6247736110852244 2201 42 -0.2248896913279239 2201 43 0.8820149291002934 2201 44 -0.7038634628999247 2201 45 1.25081162079087 2201 46 0.6217442151702102 2201 47 1.4637054779686025 2201 48 1.7024786680883253 2201 49 1.1251443460114388 2201 50 0.9399507686314337 2201 51 2.019354710174241 1101 1 -0.1721219201528541 1101 2 -0.1082585593718619 1101 3 -0.010600306037602536 1101 4 -0.0336830590158641 1101 5 0.21581827449038116 1101 6 -0.33994146327124497 1101 7 -0.4229926943688014 1101 8 0.18837397119552435 1101 9 -0.5224613816544377 1101 10 -0.3646545131562462 1101 11 0.2295715018672695 1101 12 0.4066673612546978 1101 13 -0.7970791340836811 1101 14 -2.399484112099252 1101 15 -1.0723534408197182 1101 16 -0.43719646636709086 1101 17 -0.3753438508573339 1101 18 -0.68605542918597 1101 19 0.004894566987317717 1101 20 -0.08171064652115945 1101 21 0.10423378195002186 1101 22 0.43175085792310597 1101 23 0.7311946790302386 1101 24 1.1706987273839857 1101 25 0.6328662345814848 1101 26 -0.3097706939088809 1101 27 -0.4940690719525236 1101 28 -0.6009830423442537 1101 29 -0.15054841222297455 1101 30 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0.5400386183455623 1209 10 0.7068732646215281 1209 11 0.8677659463117138 1209 12 0.9337506945880278 1209 13 0.6154208659163203 1209 14 0.2866269990118582 1209 15 -0.6140201074863825 1209 16 -0.3726131330337594 1209 17 0.8003505935871118 1209 18 0.9979723485918086 1209 19 0.3382279003206534 1209 20 1.0564837979232848 1209 21 0.7125671152833561 1209 22 -0.1300546976324526 1209 23 0.6485557901413515 1209 24 0.48180983849509573 1209 25 0.8842551234703748 1209 26 0.6624515283133405 1209 27 1.1281531502696964 1209 28 1.3997114021001877 1209 29 1.0751460322214683 1209 30 1.2040120771481213 1209 31 1.3940876391255017 1209 32 1.6173896134079442 1209 33 1.312425803942979 1209 34 0.9232643663123667 1209 35 1.2947852676355467 1209 36 -2.498621675811158 1209 37 -1.3291265751889938 1209 38 -0.9130134044533449 1209 39 0.5459015059586818 1209 40 0.20580427207620744 1209 41 -0.13286527780366697 1209 42 1.1681658642276318 1209 43 1.5903482624336291 1209 44 1.2697476482111867 1209 45 1.4959505096797585 1209 46 1.2161886596146516 1209 47 0.8991221446352711 1209 48 0.9559508903105467 1209 49 1.2612554571225516 1209 50 1.698978546409208 1209 51 2.0742158212853496 1214 1 0.8010923655614306 1214 2 -0.022161620596349962 1214 3 0.7859558164113736 1214 4 -0.30536673248525403 1214 5 0.06849684591895056 1214 6 0.2410534346879416 1214 7 0.3250940403250766 1214 8 0.09908825690980905 1214 9 -0.17424709594015297 1214 10 -0.7811085947888969 1214 11 0.5784235426835949 1214 12 0.5514377694179657 1214 13 0.15827800877346476 1214 14 -1.5346881937319026 1214 15 -0.06980242041155549 1214 16 -0.00798728269361959 1214 17 0.9722326797549101 1214 18 0.995704774895664 1214 19 0.719180281273033 1214 20 -0.11040962611299676 1214 21 0.6118868431745099 1214 22 -0.40307057064832463 1214 23 0.21907733209146585 1214 24 0.43600484983296184 1214 25 0.6239376631529154 1214 26 1.0524742040503057 1214 27 1.158992152537273 1214 28 0.6981241005128922 1214 29 0.46743628165457807 1214 30 0.4684111701186424 1214 31 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0.3654179680956773 1212 39 0.9053786301416906 1212 40 -0.31707154491726186 1212 41 0.4501412581440505 1212 42 -0.10111518152400123 1212 43 0.5315247330218611 1212 44 -0.07273601191953105 1212 45 0.2305910325555729 1212 46 0.3521363720329518 1212 47 0.39323979169409284 1212 48 -0.7172517240685394 1212 49 -0.9557380069297352 1212 50 0.05453410196476938 1212 51 0.48075176899777006 1213 1 -0.5058985158975347 1213 2 -1.1341894104356882 1213 3 -0.5431800932716442 1213 4 -0.6227788036967183 1213 5 -0.6518678957223827 1213 6 -0.9669361441223074 1213 7 -0.7561043964964611 1213 8 -0.6759877309321354 1213 9 0.0506769162179026 1213 10 -0.29218110890092674 1213 11 0.41906618271833196 1213 12 0.08552374423342002 1213 13 -0.4952174319560214 1213 14 0.08189886662415091 1213 15 -0.24522578124525296 1213 16 0.09405353363290914 1213 17 -0.9504768295807366 1213 18 -1.0989543653561853 1213 19 -0.9312756457786406 1213 20 -0.24327979545733314 1213 21 -0.21225557975210307 1213 22 -0.18726509952369952 1213 23 -1.4795765975655044 1213 24 -2.0420672300628198 1213 25 -2.9322933398865985 1213 26 -0.9640260130578184 1213 27 -2.23874992301635 1213 28 -1.2745202763868093 1213 29 -0.348021816478294 1213 30 -1.2219926509842693 1213 31 -0.784162951891048 1213 32 -0.5287096773721913 1213 33 -1.419016276435272 1213 34 -2.6634968630020595 1213 35 -0.7889026756268649 1213 36 -0.05795973727688164 1213 37 -2.9570225562764634 1213 38 -1.174006312254761 1213 39 -2.196415278911296 1213 40 -1.3946685411625666 1213 41 -1.63121043146797 1213 42 -2.1065386274981392 1213 43 -1.9344744326018315 1213 44 -1.8541294203467302 1213 45 -0.5078319962304079 1213 46 -2.287830252914894 1213 47 -0.8015870752228835 1213 48 -1.58542026808189 1213 49 -3.0583662922864363 1213 50 -2.342629018602608 1213 51 -2.2326399706768214 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 2.0 21 8.0 22 17.0 23 32.0 24 54.0 25 86.0 26 169.0 27 229.0 28 306.0 29 390.0 30 470.0 31 631.0 32 725.0 33 835.0 34 1003.0 35 1180.0 36 1457.0 37 2241.0 38 3731.0 39 10831.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.18230639937687 39.51543475587258 16.865494199155496 22.436764645595048 2 5.890068451039062 42.01336229864327 39.92704020986187 12.169529040455794 3 16.11673566422101 41.16899618805591 34.2542115833914 8.46005656433168 4 14.7108251014469 41.69365085871214 24.937492314628848 18.658031725212116 5 9.230643111858015 39.7712833545108 39.94343566831988 11.054637865311308 6 32.04902242078944 48.25593310652949 10.534082059269583 9.160962413411484 7 10.284051317784973 31.303029060950117 31.688322334713288 26.724597286551628 8 18.358814608353484 48.313317211132514 21.240316432348237 12.087551748165758 9 15.690453744312826 18.01860884534984 17.55543714391114 48.7355002664262 10 9.35770791490757 33.88531376808624 38.656392179366314 18.100586137639873 11 23.133991884248065 21.756773373775466 25.687584539082675 29.4216502028938 12 14.026314710825103 21.551830143050374 37.09472476124114 27.327130384883386 13 36.85699061360003 13.77218510472599 29.99959011353855 19.371234168135427 14 19.645858097307045 19.522892158871993 23.84309546255687 36.98815428126409 15 29.019961470672623 18.473582817559535 27.261548551051355 25.24490716071648 16 27.548469074066485 26.777882526540147 29.511825224412835 16.16182317498053 17 17.396294474504018 35.45663223479259 35.77635677979997 11.370716510903426 18 33.7951387465672 18.41209984834201 31.32352338402263 16.469238021068165 19 14.66573759068738 42.73476247079559 22.15846210599664 20.441037832520394 20 14.36652047382875 22.199450752141654 35.643726687707506 27.79030208632209 21 8.300200844366111 37.56609419190884 22.986432758126 31.147272205599048 22 13.981227200065582 39.49256056072468 22.318317825962207 24.20789441324753 23 8.398573595114152 20.490224207894414 27.19186785260483 43.919334344386606 24 18.969545435914252 39.0826740992745 28.700250030741486 13.247530434069763 25 18.543263516006068 32.003934910029926 23.95376480714842 25.499036766815593 26 19.781120629585605 26.37209492970447 34.70098782637209 19.14579661433783 27 21.851047259909002 32.78681805139976 32.99995901135386 12.362175677337378 28 11.521908431364512 20.309874164856335 33.229495429765954 34.9387219740132 29 12.57531663729147 24.25708078862155 48.546952494159115 14.620650079927861 30 14.969667158550582 30.218068535825545 34.64912280701755 20.16314149860633 31 24.691560437758742 24.425134237816128 34.05336721728081 16.829938107144322 32 12.931917858753126 34.28290363569291 34.76247079558962 18.02270770996434 33 12.841742837234085 30.51604705496577 44.65303111038242 11.989178997417715 34 9.037996474976431 28.913390990695575 35.56584826003197 26.482764274296024 35 8.099356478255523 31.2661392794196 32.70893962372423 27.925564618600646 36 10.279952453170472 26.07287781284584 45.53428700250031 18.112882731483378 37 7.660777964503833 39.677009468377264 34.188629749559375 18.473582817559535 38 11.42763454523097 25.326884453006514 43.169242119932775 20.076238881829735 39 9.03389761036193 27.26564741566586 39.16875025617904 24.531704717793172 40 8.767471410419315 24.92109685617084 40.123785711357954 26.18764602205189 41 9.546255687174652 23.990654588678936 37.81202606877895 28.651063655367466 42 18.469483952945033 24.646472926999223 34.795261712505635 22.08878140755011 43 7.685371152190844 26.097471000532853 41.47231216952904 24.744845677747264 44 7.49272451530926 35.68471533385252 38.83264335778989 17.989916793048323 45 7.919006435217445 27.925564618600646 39.29991392384309 24.85551502233881 46 17.28901094396852 26.097471000532853 32.90978398983482 23.703734065663813 47 10.591466163872608 27.21646104029184 31.2661392794196 30.925933516415956 48 6.968069844653031 24.3677501332131 25.093249169979913 43.57093085215396 49 21.49444603844735 28.007541910890684 27.69602820018855 22.80198385047342 50 8.02557691519449 26.298315366643436 35.20514817395581 30.470959544206256 51 9.743001188670739 28.25757265237529 21.920727958355535 40.07869820059843 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 244.0 1 135.0 2 26.0 3 47.0 4 68.0 5 74.5 6 81.0 7 98.5 8 116.0 9 93.5 10 71.0 11 122.0 12 173.0 13 281.0 14 389.0 15 408.5 16 428.0 17 311.0 18 194.0 19 156.5 20 119.0 21 186.0 22 253.0 23 174.0 24 95.0 25 103.5 26 191.0 27 270.0 28 233.5 29 197.0 30 312.0 31 427.0 32 714.5 33 1002.0 34 1895.5 35 2789.0 36 2933.5 37 3078.0 38 3607.5 39 4137.0 40 3629.5 41 3122.0 42 2404.5 43 1687.0 44 1513.0 45 1339.0 46 886.5 47 434.0 48 442.5 49 451.0 50 1609.5 51 2768.0 52 1441.0 53 114.0 54 92.0 55 70.0 56 57.0 57 44.0 58 44.0 59 44.0 60 34.0 61 24.0 62 19.0 63 14.0 64 12.5 65 11.0 66 7.0 67 3.0 68 1.5 69 0.0 70 0.0 71 0.0 72 0.5 73 1.0 74 1.0 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01639545845800713 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.004098864614501783 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.004098864614501783 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 24397.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.547671543502794 #Duplication Level Percentage of deduplicated Percentage of total 1 83.43801036802469 29.660269868059224 2 8.046266886676236 5.720521048778593 3 2.201039837502349 2.3472552359309473 4 1.0356016060693978 1.4725290296991567 5 0.7099714628374973 1.2618916183103779 6 0.5104909554311463 1.0888058885757188 7 0.38594984941364896 0.9603732935454532 8 0.39839289563867764 1.1329551839542957 9 0.19919569621594949 0.6372848863767542 >10 2.4525945397133615 18.056386615049988 >50 0.3361423873376107 8.236013462318445 >100 0.273893797089905 18.52992389399642 >500 0.0 0.0 >1k 0.012449718049541137 10.895789975404629 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 2462 10.09140468090339 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC 448 1.836291347296799 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC 320 1.3116366766405705 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGAG 318 1.303438947411567 No Hit AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC 306 1.2542525720375457 No Hit CAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAG 247 1.0124195597819403 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 231 0.9468377259499119 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACA 222 0.9099479444193959 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 216 0.8853547567323851 No Hit TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 211 0.8648604336598762 No Hit GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC 192 0.7869820059843423 No Hit AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA 159 0.6517194737057835 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACACA 137 0.5615444521867442 No Hit AAAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCC 134 0.549247858343239 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACATC 131 0.5369512644997336 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 129 0.52875353527073 No Hit TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT 123 0.5041603475837193 RNA PCR Primer, Index 27 (95% over 21bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 116 0.47546829528220685 No Hit TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT 114 0.4672705660532033 No Hit TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 112 0.45907283682419975 No Hit TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC 110 0.4508751075951961 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACA 106 0.434479649137189 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCC 105 0.4303807845226872 No Hit AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC 98 0.40168873222117474 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 90 0.36889781530516047 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAACTGTCTCTTATA 89 0.3647989506906587 No Hit TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA 87 0.3566012214616551 No Hit AAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAG 83 0.340205763003648 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAACTGTCTCTTAT 79 0.32381030454564086 No Hit AAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCCCA 78 0.3197114399311391 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG 77 0.31561257531663733 No Hit AAAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGA 72 0.2951182522441284 No Hit AAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCG 71 0.2910193876296266 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACACATC 71 0.2910193876296266 No Hit GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA 70 0.2869205230151248 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAACTGTCTCTTATAC 69 0.282821658400623 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCGA 69 0.282821658400623 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.282821658400623 No Hit GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC 63 0.2582284707136123 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTC 63 0.2582284707136123 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAACTGTCTCTTATACAC 62 0.25412960609911056 No Hit GTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAG 61 0.2500307414846088 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCG 61 0.2500307414846088 No Hit AAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGC 57 0.23363528302660164 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 56 0.22953641841209987 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTCCGA 56 0.22953641841209987 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACATC 55 0.22543755379759806 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 52 0.2131409599540927 No Hit TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC 52 0.2131409599540927 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51 0.20904209533959095 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAACTGTCTCTTA 50 0.20494323072508913 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 49 0.20084436611058737 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGA 49 0.20084436611058737 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.19264663688158382 No Hit AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATAC 46 0.188547772267082 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46 0.188547772267082 No Hit AAAAAGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACATCTCCGA 45 0.18444890765258024 No Hit GTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCA 44 0.18035004303807847 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44 0.18035004303807847 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGA 43 0.17625117842357665 No Hit TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC 41 0.1680534491945731 No Hit AAAAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTC 40 0.1639545845800713 No Hit AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATC 40 0.1639545845800713 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTC 39 0.15985571996556955 No Hit AAAAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCT 39 0.15985571996556955 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACAC 38 0.15575685535106776 No Hit TTTTTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT 38 0.15575685535106776 No Hit TTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACAC 38 0.15575685535106776 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCAAACTGTCTCTTATACACA 38 0.15575685535106776 No Hit CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 37 0.15165799073656597 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37 0.15165799073656597 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAACTGTCTCTTATACA 36 0.1475591261220642 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGA 35 0.1434602615075624 No Hit TCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATC 35 0.1434602615075624 No Hit TTTTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACA 35 0.1434602615075624 No Hit TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACAC 35 0.1434602615075624 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTC 35 0.1434602615075624 No Hit AAAAAGTACTCTGCGTTGATACCACTCTGTCTCTTATACACATCTCCGAGC 34 0.13936139689306062 No Hit GTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTCCGAGC 34 0.13936139689306062 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAACTGTCTCTTATACA 34 0.13936139689306062 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACAC 34 0.13936139689306062 No Hit AAAAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACA 33 0.13526253227855886 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33 0.13526253227855886 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAACTGTCTCTTATACAC 33 0.13526253227855886 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGA 32 0.13116366766405704 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGACTGTCTCTTATACACATC 32 0.13116366766405704 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAACTGTCTCTTATAC 32 0.13116366766405704 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 31 0.12706480304955528 No Hit AAAAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCG 30 0.12296593843505348 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAACTGTCTCTTATAC 30 0.12296593843505348 No Hit TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACAC 30 0.12296593843505348 No Hit TTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACAT 29 0.11886707382055171 No Hit TTTTTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATAC 29 0.11886707382055171 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCACTGTCTCTTATACACATC 29 0.11886707382055171 No Hit AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA 29 0.11886707382055171 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 29 0.11886707382055171 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCCAAACTGTCTCTTATACACATC 28 0.11476820920604994 No Hit AAAAAAAAGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACATCTC 27 0.11066934459154813 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTACAAACTGTCTCTTATACACA 27 0.11066934459154813 No Hit TTTTTTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATA 27 0.11066934459154813 No Hit AAAAAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCT 26 0.10657047997704636 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAACTGTCTC 26 0.10657047997704636 No Hit TGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATC 26 0.10657047997704636 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26 0.10657047997704636 No Hit TTTTTTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA 26 0.10657047997704636 No Hit TTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACA 26 0.10657047997704636 No Hit TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACA 26 0.10657047997704636 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAACTGTCTCTTATACACA 26 0.10657047997704636 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCT 25 0.10247161536254457 No Hit TTTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACAC 25 0.10247161536254457 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAACTGTCTCTTA 25 0.10247161536254457 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.008197729229003565 3 0.0 0.0 0.012296593843505349 4 0.0 0.0 0.01639545845800713 5 0.0 0.0 0.01639545845800713 6 0.0 0.0 0.01639545845800713 7 0.0 0.0 0.020494323072508914 8 0.0 0.0 0.024593187687010697 9 0.0 0.0 0.036889781530516044 10 0.0 0.0 0.049186375374021395 11 0.0 0.0 0.05328523998852318 12 0.0 0.0 0.05738410460302496 13 0.0 0.0 0.06558183383202852 14 0.0 0.0 0.06558183383202852 15 0.0 0.0 0.09427388613354101 16 0.0 0.0 0.11476820920604992 17 0.0 0.0 0.13936139689306062 18 0.0 0.0 0.1639545845800713 19 0.0 0.0 0.18444890765258024 20 0.0 0.0 0.2131409599540927 21 0.0 0.0 0.45497397220969793 22 0.0 0.0 0.7214001721523138 23 0.0 0.0 1.512481042751158 24 0.0 0.0 3.152026888551871 25 0.0 0.0 3.438947411566996 26 0.0 0.0 5.906463909497069 27 0.0 0.0 6.7959175308439566 28 0.0 0.0 10.029921711685864 29 0.0 0.0 12.190023363528303 30 0.0 0.0 13.546747550928393 31 0.0 0.0 18.83018403902119 32 0.0 0.0 20.96979136779112 33 0.0 0.0 22.613436078206337 34 0.0 0.0 26.98282575726524 35 0.0 0.0 29.089642169119156 36 0.0 0.0 34.97561175554372 37 0.0 0.0 38.05385908103455 38 0.0 0.0 41.02963479116285 39 0.0 0.0 44.00131163667664 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTAGGA 25 3.779109E-5 45.000004 2 GTAGGAC 25 3.779109E-5 45.000004 3 CTGTAGG 25 3.779109E-5 45.000004 1 TGCCGTC 325 0.0 41.53846 45 CCTATCT 320 0.0 41.484375 34 CTATCTC 320 0.0 41.484375 35 ATCCTAT 315 0.0 41.42857 32 TCCTATC 315 0.0 41.42857 33 GATCCTA 315 0.0 41.42857 31 ATCTCGT 330 0.0 40.90909 37 ATGCCGT 330 0.0 40.90909 44 ACTAGAT 330 0.0 40.90909 27 AGACTAG 330 0.0 40.90909 25 GAGACTA 330 0.0 40.90909 24 GACTAGA 330 0.0 40.90909 26 TATCTCG 325 0.0 40.84615 36 TAGATCC 325 0.0 40.84615 29 CCACGAG 340 0.0 40.367645 20 CACGAGA 340 0.0 40.367645 21 ACGAGAC 340 0.0 40.367645 22 >>END_MODULE