FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_23.3410000000698b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_23.3410000000698b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24397
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC246210.09140468090339No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCC4481.836291347296799No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC3201.3116366766405705No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGAG3181.303438947411567No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC3061.2542525720375457No Hit
CAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAG2471.0124195597819403No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2310.9468377259499119No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACA2220.9099479444193959No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.8853547567323851No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA2110.8648604336598762No Hit
GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC1920.7869820059843423No Hit
AAAAAGTACTCTGCGTTGATACCCTGTCTCTTATACACATCTCCGAGCCCA1590.6517194737057835No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACACA1370.5615444521867442No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCC1340.549247858343239No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACACATC1310.5369512644997336No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1290.52875353527073No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT1230.5041603475837193RNA PCR Primer, Index 27 (95% over 21bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.47546829528220685No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGT1140.4672705660532033No Hit
TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA1120.45907283682419975No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC1100.4508751075951961No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACA1060.434479649137189No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCC1050.4303807845226872No Hit
AAAAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCAC980.40168873222117474No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.36889781530516047No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAACTGTCTCTTATA890.3647989506906587No Hit
TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACA870.3566012214616551No Hit
AAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAG830.340205763003648No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAACTGTCTCTTAT790.32381030454564086No Hit
AAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCCCA780.3197114399311391No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCG770.31561257531663733No Hit
AAAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGA720.2951182522441284No Hit
AAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCG710.2910193876296266No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACACATC710.2910193876296266No Hit
GGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCA700.2869205230151248No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAACTGTCTCTTATAC690.282821658400623No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTCCGA690.282821658400623No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.282821658400623No Hit
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC630.2582284707136123No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCTC630.2582284707136123No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAACTGTCTCTTATACAC620.25412960609911056No Hit
GTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAG610.2500307414846088No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCG610.2500307414846088No Hit
AAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGC570.23363528302660164No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.22953641841209987No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTCCGA560.22953641841209987No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAACTGTCTCTTATACACATC550.22543755379759806No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.2131409599540927No Hit
TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC520.2131409599540927No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT510.20904209533959095No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAACTGTCTCTTA500.20494323072508913No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.20084436611058737No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTCCGA490.20084436611058737No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.19264663688158382No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATAC460.188547772267082No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.188547772267082No Hit
AAAAAGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACATCTCCGA450.18444890765258024No Hit
GTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGAGCCCA440.18035004303807847No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.18035004303807847No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATCTCCGA430.17625117842357665No Hit
TTTCGAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACATC410.1680534491945731No Hit
AAAAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCTC400.1639545845800713No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATC400.1639545845800713No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTCTGTCTCTTATACACATCTC390.15985571996556955No Hit
AAAAAAAGTACTCTGCGTTGATACCACTGCTTCTGTCTCTTATACACATCT390.15985571996556955No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACAC380.15575685535106776No Hit
TTTTTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTAT380.15575685535106776No Hit
TTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACAC380.15575685535106776No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCAAACTGTCTCTTATACACA380.15575685535106776No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC370.15165799073656597No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.15165799073656597No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAACTGTCTCTTATACA360.1475591261220642No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATCTCCGA350.1434602615075624No Hit
TCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATC350.1434602615075624No Hit
TTTTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACA350.1434602615075624No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACAC350.1434602615075624No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTC350.1434602615075624No Hit
AAAAAGTACTCTGCGTTGATACCACTCTGTCTCTTATACACATCTCCGAGC340.13936139689306062No Hit
GTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACACATCTCCGAGC340.13936139689306062No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAACTGTCTCTTATACA340.13936139689306062No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCGAAACTGTCTCTTATACAC340.13936139689306062No Hit
AAAAAAAGTACTCTGCGTTGATACCACTGCTTGGAAACTGTCTCTTATACA330.13526253227855886No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.13526253227855886No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAACTGTCTCTTATACAC330.13526253227855886No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGA320.13116366766405704No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGACTGTCTCTTATACACATC320.13116366766405704No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAACTGTCTCTTATAC320.13116366766405704No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA310.12706480304955528No Hit
AAAAAAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCG300.12296593843505348No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAACTGTCTCTTATAC300.12296593843505348No Hit
TTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTCTGTCTCTTATACAC300.12296593843505348No Hit
TTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACAT290.11886707382055171No Hit
TTTTTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATAC290.11886707382055171No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCACTGTCTCTTATACACATC290.11886707382055171No Hit
AAAGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGA290.11886707382055171No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.11886707382055171No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCCAAACTGTCTCTTATACACATC280.11476820920604994No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCCTGTCTCTTATACACATCTC270.11066934459154813No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTACAAACTGTCTCTTATACACA270.11066934459154813No Hit
TTTTTTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATA270.11066934459154813No Hit
AAAAAAAAAAGTACTCTGCGTTGATACCACTGCTGTCTCTTATACACATCT260.10657047997704636No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAACTGTCTC260.10657047997704636No Hit
TGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATC260.10657047997704636No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.10657047997704636No Hit
TTTTTTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA260.10657047997704636No Hit
TTTTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACA260.10657047997704636No Hit
TTTCCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACA260.10657047997704636No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAACTGTCTCTTATACACA260.10657047997704636No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCT250.10247161536254457No Hit
TTTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACAC250.10247161536254457No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAACTGTCTCTTA250.10247161536254457No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA253.779109E-545.0000042
GTAGGAC253.779109E-545.0000043
CTGTAGG253.779109E-545.0000041
TGCCGTC3250.041.5384645
CCTATCT3200.041.48437534
CTATCTC3200.041.48437535
ATCCTAT3150.041.4285732
TCCTATC3150.041.4285733
GATCCTA3150.041.4285731
ATCTCGT3300.040.9090937
ATGCCGT3300.040.9090944
ACTAGAT3300.040.9090927
AGACTAG3300.040.9090925
GAGACTA3300.040.9090924
GACTAGA3300.040.9090926
TATCTCG3250.040.8461536
TAGATCC3250.040.8461529
CCACGAG3400.040.36764520
CACGAGA3400.040.36764521
ACGAGAC3400.040.36764522
Produced by FastQC (version 0.11.2)