H23NVBCXX l02n01 48h_22.3410000000697e.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 48h_22.3410000000697e.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14476
Sequences flagged as poor quality	0
Sequence length	51
%GC	32

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	777	5.3675048355899415	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	362	2.5006907985631392	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	279	1.9273279911577783	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	171	1.1812655429676706	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	163	1.1260016579165515	No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	115	0.794418347609837	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	108	0.7460624481901078	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT	92	0.6355346780878696	RNA PCR Primer, Index 27 (95% over 21bp)
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	77	0.5319148936170213	No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	76	0.5250069079856314	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76	0.5250069079856314	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	64	0.44211108040895275	No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	60	0.41447913788339324	No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	60	0.41447913788339324	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	46	0.3177673390439348	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43	0.2970433821497651	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA	42	0.2901353965183753	No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39	0.26941143962420555	No Hit
GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	37	0.2555954683614258	No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	32	0.22105554020447638	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28	0.19342359767891684	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA	28	0.19342359767891684	No Hit
GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	26	0.17960762641613703	No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA	26	0.17960762641613703	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24	0.16579165515335728	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAA	22	0.1519756838905775	No Hit
ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	22	0.1519756838905775	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA	21	0.14506769825918764	No Hit
GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20	0.13815971262779772	No Hit
TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19	0.13125172699640786	No Hit
GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	19	0.13125172699640786	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18	0.12434374136501797	No Hit
CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18	0.12434374136501797	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	16	0.11052777010223819	No Hit
ATGAGAAACATCCACTTGACGACTTGAAAAATGACGAAATCACTAAAAAAC	16	0.11052777010223819	No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	15	0.10361978447084831	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	15	0.10361978447084831	No Hit
ATGAAAAAGGAGACATTAAAACAGATACTGCAGAAAATCAAAGGATCATTA	15	0.10361978447084831	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
ATCCTAT	60	44.999996	32
CCTATCT	60	44.999996	34
TCCTATC	60	44.999996	33
GATCCTA	60	44.999996	31
CTAGATC	60	44.999996	28
ACTAGAT	60	44.999996	27
AGACTAG	60	44.999996	25
AGATCCT	60	44.999996	30
TAGATCC	60	44.999996	29
CTATCTC	60	44.999996	35
GACTAGA	60	44.999996	26
GTATGCC	80	42.1875	42
TATGCCG	80	42.1875	43
ACGAGAC	80	42.1875	22
CGTATGC	80	42.1875	41
TCGTATG	80	42.1875	40
CTCGTAT	80	42.1875	39
ATCTCGT	75	42.000004	37
TATCTCG	75	42.000004	36
CGAGACT	70	41.785717	23

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content