##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_22.3410000000697e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14476 Sequences flagged as poor quality 0 Sequence length 51 %GC 32 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.37572533849129 38.0 38.0 38.0 32.0 38.0 2 36.29676706272451 38.0 38.0 38.0 32.0 38.0 3 36.286405084277426 38.0 38.0 38.0 32.0 38.0 4 36.252210555402044 38.0 38.0 38.0 32.0 38.0 5 36.3051948051948 38.0 38.0 38.0 32.0 38.0 6 38.233558994197296 40.0 38.0 40.0 38.0 40.0 7 38.10762641613705 40.0 38.0 40.0 38.0 40.0 8 38.0611356728378 40.0 38.0 40.0 38.0 40.0 9 38.056576402321085 40.0 38.0 40.0 32.0 40.0 10 38.01208897485493 40.0 38.0 40.0 32.0 40.0 11 37.87068250898038 40.0 38.0 40.0 32.0 40.0 12 37.814037026802986 40.0 38.0 40.0 32.0 40.0 13 37.6888643271622 40.0 38.0 40.0 32.0 40.0 14 37.72202265819287 40.0 38.0 40.0 32.0 40.0 15 37.73051948051948 40.0 38.0 40.0 32.0 40.0 16 37.708828405636915 40.0 38.0 40.0 32.0 40.0 17 37.66869300911854 40.0 38.0 40.0 32.0 40.0 18 37.67781155015197 40.0 38.0 40.0 32.0 40.0 19 37.82861287648522 40.0 38.0 40.0 32.0 40.0 20 37.8161094224924 40.0 38.0 40.0 32.0 40.0 21 37.803744128212216 40.0 38.0 40.0 32.0 40.0 22 37.8648107211937 40.0 38.0 40.0 32.0 40.0 23 37.81645482177397 40.0 38.0 40.0 32.0 40.0 24 37.77873722022658 40.0 38.0 40.0 32.0 40.0 25 37.695357833655706 40.0 38.0 40.0 32.0 40.0 26 37.60258358662614 40.0 38.0 40.0 32.0 40.0 27 37.571152252003316 40.0 38.0 40.0 32.0 40.0 28 37.51429953025698 40.0 38.0 40.0 32.0 40.0 29 37.44100580270793 40.0 38.0 40.0 32.0 40.0 30 37.290273556231 40.0 38.0 40.0 32.0 40.0 31 37.197775628626694 40.0 38.0 40.0 32.0 40.0 32 37.06348438795247 40.0 38.0 40.0 32.0 40.0 33 36.96974302293451 40.0 38.0 40.0 32.0 40.0 34 36.91095606521139 40.0 38.0 40.0 32.0 40.0 35 36.80077369439071 40.0 38.0 40.0 32.0 40.0 36 36.655982315556784 40.0 38.0 40.0 27.0 40.0 37 36.45502901353965 40.0 38.0 40.0 27.0 40.0 38 36.4001105277701 40.0 38.0 40.0 27.0 40.0 39 36.20779220779221 40.0 38.0 40.0 27.0 40.0 40 36.08040895274938 40.0 38.0 40.0 27.0 40.0 41 36.11108040895275 40.0 38.0 40.0 27.0 40.0 42 36.02597402597402 38.0 38.0 40.0 27.0 40.0 43 35.94245647969052 40.0 38.0 40.0 27.0 40.0 44 35.833724785852446 38.0 38.0 40.0 27.0 40.0 45 35.69287095882841 38.0 38.0 40.0 27.0 40.0 46 35.5387537993921 38.0 38.0 40.0 27.0 40.0 47 35.46974302293451 38.0 38.0 40.0 13.0 40.0 48 35.30761260016579 38.0 38.0 40.0 13.0 40.0 49 35.17933130699088 38.0 38.0 40.0 13.0 40.0 50 35.15190660403427 38.0 38.0 40.0 13.0 40.0 51 34.63401492124896 38.0 32.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.40841026359561994 2201 2 0.48573818766944754 2201 3 0.5138237444645739 2201 4 -0.10413039059979923 2201 5 0.24694495828494922 2201 6 0.26397814443067347 2201 7 0.07955428147092647 2201 8 0.896079776798345 2201 9 1.4384301879358432 2201 10 1.2667997323813438 2201 11 1.454305420124932 2201 12 0.32605307464403666 2201 13 0.5287206942974692 2201 14 1.102262909439979 2201 15 1.5786847029700652 2201 16 1.6475092388704908 2201 17 1.9780409578841542 2201 18 1.9098268274999484 2201 19 1.7481759943676138 2201 20 1.5132681087026114 2201 21 1.9683445822820147 2201 22 1.7937466753539368 2201 23 1.5502682129110354 2201 24 1.620257263357729 2201 25 1.5701954204296982 2201 26 1.7011597785091794 2201 27 0.6363337802547875 2201 28 -0.14054617650889156 2201 29 0.7610462573511683 2201 30 1.7858706763180052 2201 31 2.379178481964587 2201 32 1.1534455392336938 2201 33 2.331140305089697 2201 34 2.2328073955287238 2201 35 2.5067653082497543 2201 36 1.8313842976989108 2201 37 1.3114653058727725 2201 38 2.770594055229928 2201 39 2.1110120742787615 2201 40 2.202002985974815 2201 41 2.7209518899598777 2201 42 3.4706441482319903 2201 43 2.9649867526389 2201 44 3.4981930352818935 2201 45 3.426512911988887 2201 46 3.6878995189520722 2201 47 3.853952422758688 2201 48 4.2813406402408205 2201 49 3.390985556952515 2201 50 4.3516509988232315 2201 51 4.114296241797682 1101 1 -0.9540897364043772 1101 2 0.5607381876694504 1101 3 0.9638237444645767 1101 4 0.20836960940020077 1101 5 -0.6155550417150479 1101 6 -1.8735218555693294 1101 7 -0.3204457185290721 1101 8 0.646079776798345 1101 9 -0.8740698120641568 1101 10 -0.12070026761865904 1101 11 0.8793054201249291 1101 12 -0.8864469253559619 1101 13 0.42872069429746773 1101 14 0.4022629094399761 1101 15 0.45368470297006525 1101 16 -1.8399907611295063 1101 17 0.7530409578841528 1101 18 0.40982682749994837 1101 19 0.6231759943676138 1101 20 0.9632681087026143 1101 21 0.4308445822820133 1101 22 -0.843753324646066 1101 23 1.2752682129110369 1101 24 -0.6422427366422738 1101 25 -0.31730457957030467 1101 26 1.5261597785091823 1101 27 0.9238337802547889 1101 28 1.1844538234911113 1101 29 -0.4514537426488303 1101 30 1.6858706763180038 1101 31 -0.6833215180354131 1101 32 -2.121554460766305 1101 33 -1.3438596949103 1101 34 1.345307395528721 1101 35 -0.13073469175024854 1101 36 1.5313842976989136 1101 37 -0.2010346941272303 1101 38 1.1080940552299268 1101 39 -0.86398792572124 1101 40 -0.19799701402518366 1101 41 0.9084518899598777 1101 42 2.0706441482319917 1101 43 -1.9725132473610998 1101 44 1.2981930352818907 1101 45 -0.42348708801111457 1101 46 -0.6996004810479306 1101 47 1.7789524227586853 1101 48 0.6313406402408219 1101 49 -1.421514443047485 1101 50 -2.12334900117677 1101 51 -3.085703758202321 2202 1 0.15841026359561994 2202 2 -1.0142618123305525 2202 3 -0.6861762555354218 2202 4 -1.304130390599802 2202 5 -0.053055041715047935 2202 6 0.6139781444306678 2202 7 -3.1204457185290693 2202 8 -0.1539202232016521 2202 9 -0.9615698120641554 2202 10 0.4167997323813424 2202 11 -2.295694579875068 2202 12 -2.323946925355962 2202 13 -0.6712793057025337 2202 14 -0.3477370905600239 2202 15 -2.971315297029932 2202 16 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44 -2.432576195487343 1214 45 -1.2119486264726547 1214 46 -0.204408173355624 1214 47 0.19241396122022536 1214 48 0.3428791017792818 1214 49 -0.1474759815090252 1214 50 1.0978048449770768 1214 51 -0.12416529666386111 1215 1 -0.006974351788990418 1215 2 0.5318920338232971 1215 3 -0.09386856322772985 1215 4 0.5304849940155876 1215 5 -0.37997811863812814 1215 6 0.7755166059691305 1215 7 0.968015819932468 1215 8 0.7806951614137319 1215 9 0.5153532648589234 1215 10 0.9898766554582608 1215 11 0.9081515739710824 1215 12 1.0991299977209579 1215 13 0.9479514635282342 1215 14 0.8445706017476695 1215 15 0.6171462414316053 1215 16 0.8090477004089536 1215 17 -0.04503596519276698 1215 18 0.21751913519225496 1215 19 1.0174067635983803 1215 20 0.9055758010103077 1215 21 0.9452676592050935 1215 22 0.9399005215077807 1215 23 1.1502682129110369 1215 24 0.4587188018192663 1215 25 0.7548108050450821 1215 26 -0.8680709907215842 1215 27 1.2940260879470955 1215 28 -0.05593079189350192 1215 29 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0.08083433801140671 1212 15 0.18582756011291934 1212 16 0.9903663817276396 1212 17 1.0923266721698681 1212 18 -0.34017317250005163 1212 19 -0.5018240056323862 1212 20 -0.10816046272595514 1212 21 1.154058867996298 1212 22 0.593746675353934 1212 23 0.5431253557681828 1212 24 0.6702572633577262 1212 25 -0.27266172242745057 1212 26 -1.1345545072051024 1212 27 -0.6208090768880652 1212 28 -0.2976890336517428 1212 29 0.0039034002083155883 1212 30 -1.0641293236819962 1212 31 -2.1922500894639825 1212 32 -0.3179830321948742 1212 33 0.7097117336611305 1212 34 0.17566453838586682 1212 35 -0.26466326317881794 1212 36 0.7456700119846289 1212 37 -0.674248979841515 1212 38 -0.36512023048435793 1212 39 -1.1675593542926705 1212 40 -0.19799701402518366 1212 41 -0.7790481100401223 1212 42 -7.844231965776771E-4 1212 43 -2.142156104503954 1212 44 -0.7732355361466787 1212 45 0.6122271977031701 1212 46 0.0593280903806388 1212 47 -0.5960475772413147 1212 48 -0.22580221690203217 1212 49 0.3909855569525149 1212 50 -0.7840632868910546 1212 51 1.4500105275119637 1213 1 -0.6832564030710415 1213 2 -0.3142618123305496 1213 3 -1.3278429222020875 1213 4 -0.5624637239331349 1213 5 -0.30305504171504793 1213 6 -2.5610218555693294 1213 7 -0.8621123851957364 1213 8 -0.8955868898683192 1213 9 0.06343018793584321 1213 10 -1.808200267618659 1213 11 -2.745694579875071 1213 12 -1.198946925355962 1213 13 -1.5712793057025323 1213 14 -0.6394037572266882 1213 15 -2.3796486303632705 1213 16 -0.8191574277961706 1213 17 -2.580292375449183 1213 18 -2.631839839166716 1213 19 -2.9184906722990505 1213 20 -1.4117318912973857 1213 21 -2.2566554177179867 1213 22 -2.5729199913127303 1213 23 -2.058065120422299 1213 24 -2.0380760699756095 1213 25 -1.296471246236969 1213 26 -1.890506888157482 1213 27 -1.138666219745211 1213 28 -2.2738795098422244 1213 29 -2.0556204093154946 1213 30 -2.814129323681996 1213 31 -2.2874881847020774 1213 32 -1.0798877940996405 1213 33 -2.552193028243636 1213 34 -1.5505259378046148 1213 35 -0.8182346917502485 1213 36 -3.0102823689677507 1213 37 -3.1802013607938946 1213 38 -2.662739278103409 1213 39 -1.36398792572124 1213 40 -0.23966368069184796 1213 41 -2.0290481100401223 1213 42 -5.38768918510134 1213 43 -1.826679914027764 1213 44 -0.8684736313847736 1213 45 0.20151291198888543 1213 46 0.029566185618733698 1213 47 -1.512714243907979 1213 48 0.4230073069074862 1213 49 0.1826522236191792 1213 50 0.08498433215656576 1213 51 0.164296241797679 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 1.0 16 0.0 17 1.0 18 2.0 19 1.0 20 5.0 21 8.0 22 14.0 23 20.0 24 43.0 25 84.0 26 128.0 27 141.0 28 193.0 29 283.0 30 333.0 31 427.0 32 523.0 33 546.0 34 640.0 35 692.0 36 890.0 37 1230.0 38 2172.0 39 6099.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.6147042564953 31.88225538971808 15.68546158098397 19.817578772802655 2 9.13926499032882 31.334622823984525 42.31831997789445 17.20779220779221 3 17.04200055263885 37.15114672561481 33.344846642718984 12.462006079027356 4 10.94224924012158 31.258634982039236 35.1063829787234 22.692732799115777 5 9.215252832274109 40.28737220226582 37.13042276872064 13.366952196739431 6 23.68057474440453 48.17629179331307 14.02321083172147 14.119922630560927 7 13.477479966841669 40.97126277977342 22.699640784747167 22.851616468637744 8 18.13346228239845 44.7361149488809 21.35949157225753 15.770931196463112 9 19.6048632218845 24.150317767339043 22.754904669798286 33.489914340978174 10 12.565625863498203 38.180436584691904 30.056645482177398 19.197292069632496 11 22.61674495717049 28.564520585797183 28.226029289859078 20.592705167173253 12 18.057474440453163 30.733628074053605 32.01851340149213 19.190384084001106 13 24.336833379386572 26.381597126277978 32.14976512848853 17.13180436584692 14 16.4617297596021 27.707930367504837 29.9875656258635 25.842774247029567 15 19.97098646034816 33.49682232660956 26.568112738325507 19.96407847471677 16 18.658469190384082 36.916275214147554 27.901353965183752 16.52390163028461 17 13.829787234042554 40.4393478861564 31.18264714009395 14.5482177397071 18 19.96407847471677 30.768168002210555 36.16330478032606 13.104448742746616 19 14.375518098922354 35.41724233213595 32.48134843879525 17.725891130146447 20 13.947222989776181 30.851063829787233 35.03039513677811 20.17131804365847 21 11.108040895274938 36.37054434926775 33.05471124620061 19.4667035092567 22 11.950815142304503 41.668969328543795 33.10997513125173 13.270240397899972 23 10.96988118264714 33.199778944459794 34.48466427189831 21.345675600994753 24 17.691351201989498 36.99917104172423 34.719535783365565 10.589941972920697 25 13.366952196739431 39.86598507875104 33.15142304504007 13.615639679469465 26 15.998894722298978 35.168554849405915 36.17021276595745 12.662337662337661 27 13.028460900801326 39.83835313622547 34.64354794142028 12.489638021552915 28 10.735009671179883 33.86985355070461 34.505388228792484 20.889748549323016 29 12.109698811826473 33.690245924288476 41.668969328543795 12.531085935341254 30 13.015544041450777 38.96373056994818 34.10017271157167 13.92055267702936 31 19.079856313898865 34.36032053053329 33.372478585244544 13.187344570323296 32 12.800497374965461 39.872893064382424 34.249792760431056 13.076816800221055 33 11.287648521691075 36.21166067974579 39.61038961038961 12.890301188173527 34 12.20641061066593 35.783365570599614 34.09781707654048 17.912406742193976 35 11.881735285990604 36.16330478032606 33.28267477203647 18.672285161646865 36 11.04586902459243 37.08206686930091 38.553467808786955 13.3185962973197 37 11.391268306161924 41.5998894722299 33.17905498756563 13.829787234042554 38 12.026802984249793 36.38436032053053 37.420558165239015 14.168278529980658 39 12.696877590494612 34.954407294832826 33.172147001934235 19.176568112738327 40 13.684719535783366 33.6695219673943 38.15971262779773 14.486045869024592 41 13.774523348991435 34.28433268858801 31.838905775075986 20.10223818734457 42 17.511743575573362 34.77479966841669 33.69715390991987 14.016302846090081 43 11.467256148107213 34.65736391268306 39.18900248687483 14.686377452334899 44 11.294556507322465 39.216634429400386 34.30505664548218 15.18375241779497 45 12.061342912406742 33.66261398176292 38.408400110527765 15.86764299530257 46 16.99364465321912 33.80077369439072 32.69549599336833 16.51008565902183 47 12.040618955512572 31.997789444597956 33.36557059961315 22.59602100027632 48 11.114948880906327 32.46062448190108 32.48134843879525 23.943078198397348 49 17.463387676153634 31.85272174633877 33.42774247029566 17.25614810721194 50 10.735009671179883 33.24813484387953 37.73141751865156 18.28543796628903 51 11.108040895274938 32.01851340149213 32.239568941696604 24.633876761536335 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 373.0 1 209.0 2 45.0 3 54.5 4 64.0 5 83.5 6 103.0 7 137.5 8 172.0 9 128.5 10 85.0 11 171.0 12 257.0 13 381.5 14 506.0 15 532.0 16 558.0 17 412.0 18 266.0 19 224.5 20 183.0 21 261.5 22 340.0 23 252.0 24 164.0 25 195.5 26 333.5 27 440.0 28 539.0 29 638.0 30 908.0 31 1178.0 32 1590.5 33 2003.0 34 2012.0 35 2021.0 36 1734.0 37 1447.0 38 1154.5 39 862.0 40 657.5 41 453.0 42 356.5 43 260.0 44 235.0 45 210.0 46 166.5 47 123.0 48 180.5 49 238.0 50 593.0 51 948.0 52 539.5 53 131.0 54 95.0 55 59.0 56 49.0 57 39.0 58 34.5 59 30.0 60 25.5 61 21.0 62 15.5 63 10.0 64 9.5 65 9.0 66 6.0 67 3.0 68 2.0 69 1.0 70 3.0 71 5.0 72 3.5 73 2.0 74 1.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027631942525559547 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.006907985631389887 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 14476.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.96395004349654 #Duplication Level Percentage of deduplicated Percentage of total 1 81.63777742301954 46.504102747869744 2 10.5273491497391 11.993587821123677 3 3.32440090535909 5.681130212922896 4 1.57431039024357 3.5871575369116906 5 0.712176112302481 2.0284182241685054 6 0.524760775926271 1.793546797479035 7 0.32485188603400383 1.295339262530364 8 0.2623803682926343 1.1956977753452673 9 0.14993163898013476 0.7686628553523559 >10 0.7871411046299185 7.501580088194699 >50 0.08746012273662944 3.5942105735650784 >100 0.07496581948853952 8.526463895308838 >500 0.01249430324808992 5.530102209227853 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 777 5.3675048355899415 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 362 2.5006907985631392 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 279 1.9273279911577783 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 171 1.1812655429676706 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 163 1.1260016579165515 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 115 0.794418347609837 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 108 0.7460624481901078 No Hit TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT 92 0.6355346780878696 RNA PCR Primer, Index 27 (95% over 21bp) ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 77 0.5319148936170213 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 76 0.5250069079856314 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 76 0.5250069079856314 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 64 0.44211108040895275 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 60 0.41447913788339324 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 60 0.41447913788339324 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 46 0.3177673390439348 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43 0.2970433821497651 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 42 0.2901353965183753 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 39 0.26941143962420555 No Hit GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 37 0.2555954683614258 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32 0.22105554020447638 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28 0.19342359767891684 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 28 0.19342359767891684 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26 0.17960762641613703 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 26 0.17960762641613703 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24 0.16579165515335728 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAA 22 0.1519756838905775 No Hit ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22 0.1519756838905775 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA 21 0.14506769825918764 No Hit GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20 0.13815971262779772 No Hit TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.13125172699640786 No Hit GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.13125172699640786 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18 0.12434374136501797 No Hit CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.12434374136501797 No Hit CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 16 0.11052777010223819 No Hit ATGAGAAACATCCACTTGACGACTTGAAAAATGACGAAATCACTAAAAAAC 16 0.11052777010223819 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.10361978447084831 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 15 0.10361978447084831 No Hit ATGAAAAAGGAGACATTAAAACAGATACTGCAGAAAATCAAAGGATCATTA 15 0.10361978447084831 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.006907985631389887 3 0.0 0.0 0.006907985631389887 4 0.0 0.0 0.006907985631389887 5 0.0 0.0 0.006907985631389887 6 0.0 0.0 0.006907985631389887 7 0.0 0.0 0.006907985631389887 8 0.0 0.0 0.006907985631389887 9 0.0 0.0 0.006907985631389887 10 0.0 0.0 0.006907985631389887 11 0.0 0.0 0.006907985631389887 12 0.0 0.0 0.006907985631389887 13 0.0 0.0 0.006907985631389887 14 0.0 0.0 0.006907985631389887 15 0.0 0.0 0.006907985631389887 16 0.0 0.0 0.02072395689416966 17 0.0 0.0 0.02072395689416966 18 0.0 0.0 0.03453992815694943 19 0.0 0.0 0.04144791378833932 20 0.0 0.0 0.06907985631389886 21 0.0 0.0 0.08289582757667864 22 0.0 0.0 0.13125172699640786 23 0.0 0.0 0.2555954683614258 24 0.0 0.0 0.4075711522520033 25 0.0 0.0 0.5042829510914617 26 0.0 0.0 0.7598784194528876 27 0.0 0.0 0.8911301464492953 28 0.0 0.0 1.2227134567560098 29 0.0 0.0 1.768444321635811 30 0.0 0.0 2.1691074882564245 31 0.0 0.0 2.7839182094501242 32 0.0 0.0 3.467808786957723 33 0.0 0.0 4.0411715943630835 34 0.0 0.0 4.731970157502072 35 0.0 0.0 5.298424979276043 36 0.0 0.0 6.1688311688311686 37 0.0 0.0 7.239568941696601 38 0.0 0.0 8.358662613981762 39 0.0 0.0 9.574468085106384 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCTAT 60 0.0 44.999996 32 CCTATCT 60 0.0 44.999996 34 TCCTATC 60 0.0 44.999996 33 GATCCTA 60 0.0 44.999996 31 CTAGATC 60 0.0 44.999996 28 ACTAGAT 60 0.0 44.999996 27 AGACTAG 60 0.0 44.999996 25 AGATCCT 60 0.0 44.999996 30 TAGATCC 60 0.0 44.999996 29 CTATCTC 60 0.0 44.999996 35 GACTAGA 60 0.0 44.999996 26 GTATGCC 80 0.0 42.1875 42 TATGCCG 80 0.0 42.1875 43 ACGAGAC 80 0.0 42.1875 22 CGTATGC 80 0.0 42.1875 41 TCGTATG 80 0.0 42.1875 40 CTCGTAT 80 0.0 42.1875 39 ATCTCGT 75 0.0 42.000004 37 TATCTCG 75 0.0 42.000004 36 CGAGACT 70 0.0 41.785717 23 >>END_MODULE