FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_21.34100000006961.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_21.34100000006961.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81326
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC10721.3181516366229742No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1660.20411676462631875No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.19427981211420703No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1300.1598504783218159No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT1200.14755428768167622No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT1100.13525809704153655RNA PCR Primer, Index 27 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1050.1291100017214667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT1100.042.95395739
CGAGACT1050.042.8565623
ATCTCGT1000.042.74941637
TATGCCG1000.042.74941643
TCCTATC950.042.63099333
TATCTCG950.042.63099336
CCACGAG1150.041.08639520
GTATGCC1050.040.7137342
ATGCCGT1050.040.7137344
TGCCGTC1050.040.7137345
CTATCTC950.040.26260835
GCCCACG1200.039.37446218
TCTCGTA1200.039.37446238
ACGAGAC1200.039.37446222
TCGTATG1150.039.12989840
AGACTAG1100.038.86310225
GATCCTA1000.038.24947731
CACGAGA1250.037.79948421
CCCACGA1250.037.79948419
CGTATGC1200.037.49948541