##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_20.34100000006955.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 71637 Sequences flagged as poor quality 0 Sequence length 51 %GC 36 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.33566453089884 38.0 38.0 38.0 32.0 38.0 2 36.39062216452392 38.0 38.0 38.0 32.0 38.0 3 36.33246785878806 38.0 38.0 38.0 32.0 38.0 4 36.40285048229267 38.0 38.0 38.0 32.0 38.0 5 36.43347711378198 38.0 38.0 38.0 32.0 38.0 6 38.31372056339601 40.0 38.0 40.0 38.0 40.0 7 38.19894747127881 40.0 38.0 40.0 38.0 40.0 8 38.181191283833776 40.0 38.0 40.0 38.0 40.0 9 38.176082192163264 40.0 38.0 40.0 38.0 40.0 10 38.12695953208537 40.0 38.0 40.0 38.0 40.0 11 38.10297751162109 40.0 38.0 40.0 38.0 40.0 12 38.001661152756256 40.0 38.0 40.0 32.0 40.0 13 38.04224074151625 40.0 38.0 40.0 32.0 40.0 14 37.96972235018217 40.0 38.0 40.0 32.0 40.0 15 37.97162081047503 40.0 38.0 40.0 32.0 40.0 16 37.99699875762525 40.0 38.0 40.0 32.0 40.0 17 38.02440079847006 40.0 38.0 40.0 32.0 40.0 18 37.98609657020813 40.0 38.0 40.0 32.0 40.0 19 38.043622708935324 40.0 38.0 40.0 38.0 40.0 20 38.00449488392869 40.0 38.0 40.0 32.0 40.0 21 38.02643885143152 40.0 38.0 40.0 32.0 40.0 22 38.0213018412273 40.0 38.0 40.0 32.0 40.0 23 37.98517525859542 40.0 38.0 40.0 32.0 40.0 24 37.98918156818404 40.0 38.0 40.0 32.0 40.0 25 37.98031743372838 40.0 38.0 40.0 32.0 40.0 26 37.91593729497327 40.0 38.0 40.0 32.0 40.0 27 37.90256431732205 40.0 38.0 40.0 32.0 40.0 28 37.85842511551294 40.0 38.0 40.0 32.0 40.0 29 37.8337730502394 40.0 38.0 40.0 32.0 40.0 30 37.78918715189078 40.0 38.0 40.0 32.0 40.0 31 37.80644080572888 40.0 38.0 40.0 32.0 40.0 32 37.77129137177715 40.0 38.0 40.0 32.0 40.0 33 37.66973770537572 40.0 38.0 40.0 32.0 40.0 34 37.719670002931444 40.0 38.0 40.0 32.0 40.0 35 37.66796487848458 40.0 38.0 40.0 32.0 40.0 36 37.63788265840278 40.0 38.0 40.0 32.0 40.0 37 37.568491142845176 40.0 38.0 40.0 32.0 40.0 38 37.562781802699725 40.0 38.0 40.0 32.0 40.0 39 37.50088641344556 40.0 38.0 40.0 32.0 40.0 40 37.46520652735318 40.0 38.0 40.0 32.0 40.0 41 37.47133464550442 40.0 38.0 40.0 32.0 40.0 42 37.473763557937936 40.0 38.0 40.0 32.0 40.0 43 37.45875734606418 40.0 38.0 40.0 32.0 40.0 44 37.418401105573935 40.0 38.0 40.0 32.0 40.0 45 37.4134036880383 40.0 38.0 40.0 32.0 40.0 46 37.373871044292756 40.0 38.0 40.0 32.0 40.0 47 37.33414297081117 40.0 38.0 40.0 32.0 40.0 48 37.28661166715524 40.0 38.0 40.0 32.0 40.0 49 37.233706045758474 40.0 38.0 40.0 32.0 40.0 50 37.230690844116864 40.0 38.0 40.0 32.0 40.0 51 36.76238535952092 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.011135527673062029 2201 2 -0.29183307049967055 2201 3 0.25741422545475956 2201 4 0.7872494113110307 2201 5 0.4897224977499519 2201 6 0.4296313113940684 2201 7 0.6223864728052959 2201 8 0.9032980987606933 2201 9 1.0749917765390933 2201 10 0.5272073531265136 2201 11 0.5056379842512655 2201 12 0.28872898358618926 2201 13 0.600855004585803 2201 14 0.18037043058314595 2201 15 0.38791085061792074 2201 16 0.5596192380815452 2201 17 0.433728674945975 2201 18 0.5190992154990326 2201 19 0.8631504495294635 2201 20 -0.3043940327393386 2201 21 0.4086064765409958 2201 22 0.8141514708660793 2201 23 0.19917368303230631 2201 24 0.9609614447355099 2201 25 0.680231150653448 2201 26 0.7953289146806668 2201 27 0.5030024752755793 2201 28 0.9971388183802077 2201 29 0.6476705928246957 2201 30 0.20866533243135876 2201 31 0.9219406032187578 2201 32 0.794206699095966 2201 33 0.9481200114795598 2201 34 0.8245918319170968 2201 35 0.9742505140468296 2201 36 1.0747928433870229 2201 37 0.6825826701719819 2201 38 0.6281851393163365 2201 39 0.6305809430207816 2201 40 0.532326226752275 2201 41 0.684523837300695 2201 42 0.970432092862346 2201 43 0.144965097769159 2201 44 0.6291344482648498 2201 45 1.1120172692615071 2201 46 1.2346203622666962 2201 47 1.085958106891681 2201 48 1.2940743445870737 2201 49 0.6156661864283848 2201 50 0.9796433112286991 2201 51 0.6351954260667299 1101 1 -0.8589890630265984 1101 2 -0.32781791898452184 1101 3 -0.013419107878569037 1101 4 0.05240092646254624 1101 5 -0.1955679062904494 1101 6 -0.2294595976968381 1101 7 -0.43380039588156905 1101 8 -0.5742902850776872 1101 9 -0.9635183244710106 1101 10 0.1111719995911642 1101 11 0.09118091354419278 1101 12 0.43929716540436914 1101 13 0.14630955004034973 1101 14 0.5165446730073882 1101 15 0.11139569910276492 1101 16 0.06435408656638941 1101 17 -0.17896071899342303 1101 18 0.015311336711157253 1101 19 -0.33918540905639105 1101 20 0.005580714735408776 1101 21 -1.1409516042670873 1101 22 0.08877268298729035 1101 23 0.19254489515351736 1101 24 -0.5355663330422686 1101 25 -0.3810062230839293 1101 26 0.036174874276625246 1101 27 -0.007729847956746028 1101 28 0.030282757774145352 1101 29 -0.0644506192965153 1101 30 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15 0.41957751728458703 2202 16 -0.11746409525179047 2202 17 0.1416453416126373 2202 18 -0.3559007845009674 2202 19 -0.20726621713720306 2202 20 0.2947726339273231 2202 21 0.11985647654099552 2202 22 -0.08334852913392155 2202 23 0.43042368303230916 2202 24 0.058878111402172806 2202 25 0.39273115065344655 2202 26 0.27657891468066964 2202 27 0.37216914194224415 2202 28 -0.04911118161979289 2202 29 0.2876705928246963 2202 30 0.5057486657646919 2202 31 0.17110726988542524 2202 32 0.16045669909596683 2202 33 -0.15104665518710192 2202 34 -0.40915816808290373 2202 35 -0.766582819286505 2202 36 0.30354284338702087 2202 37 -0.16075066316135178 2202 38 0.1819351393163373 2202 39 0.3743309430207802 2202 40 0.35065956008561017 2202 41 -0.034642829365971295 2202 42 0.7916820928623451 2202 43 0.49038176443582415 2202 44 0.38705111493151634 2202 45 0.1095172692615094 2202 46 -0.039962971066636044 2202 47 0.041791440225019016 2202 48 -0.1809256554129277 2202 49 -0.47766714690494894 2202 50 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1213 26 -0.6641618260600737 1213 27 -1.0622753025022007 1213 28 -1.5091111816197937 1213 29 -1.1634405182864143 1213 30 0.017230147246174 1213 31 -1.922966804188654 1213 32 -0.8588025601632907 1213 33 -0.28475035889081113 1213 34 -1.176565575490308 1213 35 -0.9817680044716894 1213 36 -1.5509016010574186 1213 37 -1.4803802927909828 1213 38 -1.1947315273503278 1213 39 -0.9182616495718108 1213 40 -0.02785895843290831 1213 41 -0.5742724589956012 1213 42 -1.120540129359874 1213 43 -1.165914531860473 1213 44 -0.34887481099440976 1213 45 -1.0282605085162686 1213 46 -1.3207037118073828 1213 47 -0.9348752264416476 1213 48 -1.0864812109684792 1213 49 -0.7213708506086576 1213 50 -0.3814677998824152 1213 51 -0.06457309245178777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 14 1.0 15 0.0 16 0.0 17 0.0 18 3.0 19 1.0 20 5.0 21 9.0 22 37.0 23 55.0 24 102.0 25 191.0 26 320.0 27 487.0 28 667.0 29 859.0 30 1077.0 31 1417.0 32 1680.0 33 2026.0 34 2461.0 35 3051.0 36 4024.0 37 5967.0 38 11336.0 39 35861.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.06214215891635 26.468370339338083 15.718475073313783 22.751012428431782 2 10.72660012563691 26.04592727018915 45.5391917358833 17.68828086829064 3 17.386266873263818 30.615464075826736 32.76658709884557 19.231681952063877 4 12.317657076650335 22.570738584809526 39.936066557784386 25.175537780755754 5 11.161829780699918 39.3134832558594 38.58760137917557 10.937085584265114 6 26.096849393469856 43.35887879168586 18.826863213144048 11.717408601700239 7 20.637380124795847 35.7105964794729 25.474266091544873 18.177757304186386 8 19.14234264416433 45.21546128397337 22.482795203595906 13.159400868266399 9 22.319471781342042 19.773301506204895 24.14255203316722 33.76467467928585 10 13.661934475201361 32.59209626310427 36.1321663386239 17.61380292307048 11 28.703251294794295 24.635293789175382 29.218377005011657 17.443077911018666 12 20.764409453215517 27.18567220849561 34.931669388723705 17.118248949565167 13 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26 15.935899046582072 33.26493292572274 34.31327386685651 16.485894160838672 27 16.960509234055028 33.47711378198417 32.633974063682174 16.928402920278625 28 15.602272568644695 31.941594427460668 31.899716626882757 20.556416377011878 29 17.872049359967615 31.08868322235716 32.52229992880774 18.516967488867483 30 16.25137847760236 32.2640534919106 32.40504208718958 19.079525943297458 31 20.827226154082386 30.714574870527798 31.337158172452785 17.121040802937028 32 16.866982146097687 33.80376062649189 31.97370074123707 17.355556486173345 33 16.17041472981839 33.1714058377654 34.97773496935941 15.6804444630568 34 16.618507196002067 32.25567793179502 33.89030808101958 17.235506791183326 35 16.001507600820805 32.80288119267976 32.64932925722741 18.546281949272025 36 15.392883565755128 32.43994025433784 34.72228038583414 17.444895794072895 37 15.959629800242892 32.21519605790304 31.48233454778955 20.342839594064518 38 16.925611066906765 32.36316428661167 32.40504208718958 18.306182559291987 39 18.37597889358851 29.071569161187654 33.16721805770761 19.38523388751623 40 18.93434956796069 28.72258748970504 34.69575777880146 17.647305163532813 41 18.304786632606053 28.19771905579519 33.94056144171308 19.556932869885674 42 17.98651534821391 30.411658779680888 34.04106816310007 17.560757709005124 43 15.105322668453452 30.77459971802281 35.47189301617879 18.648184597344947 44 14.500886413445565 31.197565503859735 34.39563354132641 19.90591454136829 45 15.074612281362985 29.92866814634895 34.73484372600751 20.261875846280553 46 16.463559333863785 28.10977567458157 33.81492803997934 21.611736951575303 47 17.351368706115554 28.28845429038067 32.8824490137778 21.47772798972598 48 14.135153621731785 29.004564680262995 33.11417284364225 23.74610885436297 49 17.083350782416908 28.47690439298128 33.10440135684074 21.335343467761074 50 14.218909222887612 31.08449544229937 33.21607549171517 21.480519843097838 51 13.53147292449011 29.96803149386456 33.347758714558935 23.1527368670864 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 295.0 1 171.5 2 48.0 3 86.0 4 124.0 5 122.5 6 121.0 7 153.0 8 185.0 9 145.0 10 105.0 11 190.5 12 276.0 13 480.0 14 684.0 15 738.5 16 793.0 17 591.5 18 390.0 19 321.0 20 252.0 21 377.5 22 503.0 23 358.0 24 213.0 25 286.5 26 739.0 27 1118.0 28 1707.0 29 2296.0 30 3869.5 31 5443.0 32 7470.5 33 9498.0 34 11380.0 35 13262.0 36 12534.5 37 11807.0 38 9913.5 39 8020.0 40 6227.0 41 4434.0 42 3387.5 43 2341.0 44 1968.0 45 1595.0 46 1395.0 47 1195.0 48 1162.0 49 1129.0 50 1564.0 51 1999.0 52 1405.0 53 811.0 54 753.0 55 695.0 56 601.5 57 508.0 58 456.0 59 404.0 60 350.0 61 296.0 62 243.5 63 191.0 64 159.5 65 128.0 66 98.0 67 68.0 68 46.5 69 25.0 70 21.0 71 17.0 72 11.5 73 6.0 74 3.5 75 1.0 76 1.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03769002052012228 2 0.00279185337186091 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.00558370674372182 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.00279185337186091 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.00558370674372182 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 71637.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.93941505363217 #Duplication Level Percentage of deduplicated Percentage of total 1 87.48536042531201 64.6861637560375 2 8.226833070637033 12.165744499735576 3 2.062918711008268 4.575930083854326 4 0.7582565522916249 2.242601837481064 5 0.4081348694247168 1.508862675412705 6 0.23609715561980185 1.047413134941271 7 0.15928072062468857 0.8243986318617336 8 0.11909634295629228 0.7044731146572014 9 0.06124649952017246 0.40756773137437036 >10 0.43403735369252594 5.974317445065095 >50 0.026245955981900576 1.3985684570598513 >100 0.02061798119594218 2.975510514063206 >500 0.0 0.0 >1k 0.0018743617349964935 1.488448118456087 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 1074 1.4992252606893086 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 459 0.6407303488420788 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 284 0.39644317880424923 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 0.3238549911358656 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 194 0.27080977707050824 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 191 0.2666219970127169 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 184 0.2568505102112037 No Hit GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT 145 0.20240936945991597 No Hit TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT 119 0.16611527562572415 RNA PCR Primer, Index 27 (95% over 21bp) GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 118 0.16471934893979367 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 117 0.1633234222538632 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 104 0.1451763753367673 No Hit CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA 90 0.12563340173374096 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 83 0.11586191493222776 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC 82 0.1144659882462973 No Hit GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT 81 0.11307006156036684 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 80 0.11167413487443639 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 76 0.10609042813071456 No Hit GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA 73 0.10190264807292322 No Hit CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG 73 0.10190264807292322 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.001395926685930455 3 0.0 0.0 0.001395926685930455 4 0.0 0.0 0.001395926685930455 5 0.0 0.0 0.001395926685930455 6 0.0 0.0 0.001395926685930455 7 0.0 0.0 0.001395926685930455 8 0.0 0.0 0.001395926685930455 9 0.0 0.0 0.004187780057791365 10 0.0 0.0 0.00558370674372182 11 0.0 0.0 0.00558370674372182 12 0.0 0.0 0.00558370674372182 13 0.0 0.0 0.00558370674372182 14 0.0 0.0 0.00558370674372182 15 0.0 0.0 0.00558370674372182 16 0.0 0.0 0.0069796334296522745 17 0.0 0.0 0.01116741348744364 18 0.0 0.0 0.015355193545235004 19 0.0 0.0 0.01675112023116546 20 0.0 0.0 0.02233482697488728 21 0.0 0.0 0.04606558063570501 22 0.0 0.0 0.07538004104024457 23 0.0 0.0 0.14098859527897595 24 0.0 0.0 0.21776456300515096 25 0.0 0.0 0.2540586568393428 26 0.0 0.0 0.36992057177157056 27 0.0 0.0 0.41459022572134513 28 0.0 0.0 0.558370674372182 29 0.0 0.0 0.7133185365104625 30 0.0 0.0 0.8933930789954911 31 0.0 0.0 1.5327275011516395 32 0.0 0.0 2.1804374834233706 33 0.0 0.0 2.770914471571953 34 0.0 0.0 3.5679886092382427 35 0.0 0.0 4.247804905286374 36 0.0 0.0 5.3645462540307385 37 0.0 0.0 6.563647277244999 38 0.0 0.0 7.9009450423663745 39 0.0 0.0 9.369459915965214 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGTTC 25 3.8407212E-5 45.026554 8 ATCCCGT 20 6.992751E-4 44.99511 20 CGTTTCC 20 6.992751E-4 44.99511 24 CCAACGA 20 6.992751E-4 44.99511 29 AATCCCG 20 6.992751E-4 44.99511 19 AACGAAT 20 6.992751E-4 44.99511 31 CCGTTTC 20 6.992751E-4 44.99511 23 CCCGTTT 20 6.992751E-4 44.99511 22 TCCCGTT 20 6.992751E-4 44.99511 21 TCCAACG 20 6.992751E-4 44.99511 28 ACGAATG 20 6.992751E-4 44.99511 32 CAACGAA 20 6.992751E-4 44.99511 30 GCCCACG 175 0.0 41.13839 18 ACGAGAC 175 0.0 41.13839 22 ATCTCGT 170 0.0 41.024956 37 TCTCGTA 170 0.0 41.024956 38 TATCTCG 170 0.0 41.024956 36 CGAGACT 170 0.0 41.024956 23 TAGATCC 170 0.0 41.024956 29 ATCCTAT 165 0.0 40.904648 32 >>END_MODULE