FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_20.34100000006955.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_20.34100000006955.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71637
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC10741.4992252606893086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.6407303488420788No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2840.39644317880424923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.3238549911358656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1940.27080977707050824No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1910.2666219970127169No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1840.2568505102112037No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT1450.20240936945991597No Hit
TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT1190.16611527562572415RNA PCR Primer, Index 27 (95% over 21bp)
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.16471934893979367No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1170.1633234222538632No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1040.1451763753367673No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA900.12563340173374096No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT830.11586191493222776No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCC820.1144659882462973No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCAT810.11307006156036684No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT800.11167413487443639No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.10609042813071456No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTGA730.10190264807292322No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTAG730.10190264807292322No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGTTC253.8407212E-545.0265548
ATCCCGT206.992751E-444.9951120
CGTTTCC206.992751E-444.9951124
CCAACGA206.992751E-444.9951129
AATCCCG206.992751E-444.9951119
AACGAAT206.992751E-444.9951131
CCGTTTC206.992751E-444.9951123
CCCGTTT206.992751E-444.9951122
TCCCGTT206.992751E-444.9951121
TCCAACG206.992751E-444.9951128
ACGAATG206.992751E-444.9951132
CAACGAA206.992751E-444.9951130
GCCCACG1750.041.1383918
ACGAGAC1750.041.1383922
ATCTCGT1700.041.02495637
TCTCGTA1700.041.02495638
TATCTCG1700.041.02495636
CGAGACT1700.041.02495623
TAGATCC1700.041.02495629
ATCCTAT1650.040.90464832
Produced by FastQC (version 0.11.2)