FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_2.3410000000683c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_2.3410000000683c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC28828.599801390268127RNA PCR Primer, Index 27 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT302.9791459781529297RNA PCR Primer, Index 38 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCTTC121.1916583912611718RNA PCR Primer, Index 27 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC80.7944389275074478No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC70.6951340615690168No Hit
TATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCTT60.5958291956305859RNA PCR Primer, Index 27 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.5958291956305859No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.5958291956305859No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.49652432969215493No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.49652432969215493No Hit
CTTATACACATCTCCGAGCCCACGAGACATAGACAAATCTCGTATGCCGTC50.49652432969215493No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC40.3972194637537239No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC40.3972194637537239No Hit
TCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGT30.29791459781529295No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCG30.29791459781529295No Hit
TTTTTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.29791459781529295No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAGTACTCTGCGTTGATAC30.29791459781529295No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTCGAAGCAGTGGTATCAAC20.19860973187686196No Hit
TTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTCT20.19860973187686196No Hit
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC20.19860973187686196TruSeq Adapter, Index 12 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCCCAC20.19860973187686196No Hit
GATACCACTGCTTCGAAAAAAAAAAAAAAGTACTCTGCGTTGATACCACTG20.19860973187686196No Hit
GTGGTATCAACGCAGAGTACTTTTTTTCTGTCTCTTATACACATCTCCGAG20.19860973187686196No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA20.19860973187686196No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.19860973187686196No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.19860973187686196No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA20.19860973187686196No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTCTGTCTCTTATACACATCTCC20.19860973187686196No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC306.8894224E-744.99999617
GTATGCC306.8894224E-744.99999642
ATCTCGT306.8894224E-744.99999637
GCCCACG306.8894224E-744.99999618
TGCCGTC306.8894224E-744.99999645
CCACGAG306.8894224E-744.99999620
TATGCCG306.8894224E-744.99999643
GAGCCCA306.8894224E-744.99999616
CACGAGA306.8894224E-744.99999621
CCCACGA306.8894224E-744.99999619
GCTATCT306.8894224E-744.99999634
TCTCGTA306.8894224E-744.99999638
ATTGCTA306.8894224E-744.99999631
TATCTCG306.8894224E-744.99999636
CGAATTG306.8894224E-744.99999628
AGACGAA306.8894224E-744.99999625
ATGCCGT306.8894224E-744.99999644
CGTATGC306.8894224E-744.99999641
GAATTGC306.8894224E-744.99999629
TGCTATC306.8894224E-744.99999633
Produced by FastQC (version 0.11.2)