FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_19.34100000006948.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_19.34100000006948.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110656
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC3620.32713996529786005No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA2680.24219201850780797No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG207.0059975E-444.9995528
CCGAGCC551.1122211E-732.72694414
ATGCCGT551.1122211E-732.72694444
CCTATAG352.8042062E-432.1425363
TATCTCG501.9604613E-631.49968336
GTCCTAC2150.030.3622511
GGGTACC300.005116335630.0132621
TTCCAAC453.4629462E-529.99969927
ATCTCGT602.3859138E-729.99969737
CGTATGC602.3859138E-729.99969741
TAGACTC300.005127664729.9996975
CACGAGA602.3859138E-729.99969721
TATGCCG602.3859138E-729.99969743
TCTCCGA602.3859138E-729.99969711
TTGAGGA406.1310665E-428.124722
CTGTAGG2500.027.9123341
ATCTCCG654.807098E-727.6920310
CTCGTAT654.807098E-727.6920339
TCCGAGC654.807098E-727.6920313
TCTCGTA654.807098E-727.6920338
Produced by FastQC (version 0.11.2)