##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_18.3410000000693b.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30170 Sequences flagged as poor quality 0 Sequence length 51 %GC 30 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.38471992045078 38.0 38.0 38.0 32.0 38.0 2 36.32171030825323 38.0 38.0 38.0 32.0 38.0 3 36.330394431554524 38.0 38.0 38.0 32.0 38.0 4 36.35989393437189 38.0 38.0 38.0 32.0 38.0 5 36.449287371561155 38.0 38.0 38.0 32.0 38.0 6 38.34842558833278 40.0 38.0 40.0 38.0 40.0 7 38.227311899237655 40.0 38.0 40.0 38.0 40.0 8 38.24342061650646 40.0 38.0 40.0 38.0 40.0 9 38.18322837255552 40.0 38.0 40.0 38.0 40.0 10 38.15817036791515 40.0 38.0 40.0 38.0 40.0 11 38.07991382167716 40.0 38.0 40.0 32.0 40.0 12 37.9782234007292 40.0 38.0 40.0 32.0 40.0 13 37.902651640702686 40.0 38.0 40.0 32.0 40.0 14 37.87391448458734 40.0 38.0 40.0 32.0 40.0 15 37.863937686443485 40.0 38.0 40.0 32.0 40.0 16 37.88223400729201 40.0 38.0 40.0 32.0 40.0 17 37.94368578057673 40.0 38.0 40.0 32.0 40.0 18 37.898839907192574 40.0 38.0 40.0 32.0 40.0 19 38.04753065959562 40.0 38.0 40.0 32.0 40.0 20 38.06125290023202 40.0 38.0 40.0 32.0 40.0 21 38.06105402717932 40.0 38.0 40.0 32.0 40.0 22 38.112330129267484 40.0 38.0 40.0 38.0 40.0 23 38.07069937023533 40.0 38.0 40.0 32.0 40.0 24 38.0219754723235 40.0 38.0 40.0 32.0 40.0 25 38.03138879681803 40.0 38.0 40.0 32.0 40.0 26 37.96165064633742 40.0 38.0 40.0 32.0 40.0 27 37.91425256877693 40.0 38.0 40.0 32.0 40.0 28 37.949287371561155 40.0 38.0 40.0 32.0 40.0 29 37.87103082532317 40.0 38.0 40.0 32.0 40.0 30 37.84544249254226 40.0 38.0 40.0 32.0 40.0 31 37.83907855485582 40.0 38.0 40.0 32.0 40.0 32 37.806529665230364 40.0 38.0 40.0 32.0 40.0 33 37.728372555518725 40.0 38.0 40.0 32.0 40.0 34 37.75999337089824 40.0 38.0 40.0 32.0 40.0 35 37.743984090155784 40.0 38.0 40.0 32.0 40.0 36 37.675538614517734 40.0 38.0 40.0 32.0 40.0 37 37.69042094796155 40.0 38.0 40.0 32.0 40.0 38 37.65356314219423 40.0 38.0 40.0 32.0 40.0 39 37.5927411335764 40.0 38.0 40.0 32.0 40.0 40 37.50477295326483 40.0 38.0 40.0 32.0 40.0 41 37.52548889625456 40.0 38.0 40.0 32.0 40.0 42 37.41922439509447 40.0 38.0 40.0 32.0 40.0 43 37.3677494199536 40.0 38.0 40.0 32.0 40.0 44 37.29257540603248 40.0 38.0 40.0 32.0 40.0 45 37.22164401723566 40.0 38.0 40.0 32.0 40.0 46 37.08054358634405 40.0 38.0 40.0 32.0 40.0 47 36.97391448458734 40.0 38.0 40.0 32.0 40.0 48 36.90649651972158 40.0 38.0 40.0 32.0 40.0 49 36.792542260523696 40.0 38.0 40.0 32.0 40.0 50 36.69937023533311 40.0 38.0 40.0 27.0 40.0 51 36.51657275439178 40.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.07136319321910634 2201 2 0.5138816988094561 2201 3 -0.2960423610688565 2201 4 -0.181296834183712 2201 5 -0.7261939314594628 2201 6 -0.6133928292326658 2201 7 -0.46275532936438424 2201 8 -0.32409015849061973 2201 9 0.23488629652977977 2201 10 0.19412262897076715 2201 11 0.693986995509583 2201 12 0.3986296120187589 2201 13 -0.2085933678454026 2201 14 -0.7571644071317181 2201 15 -0.5869779245019018 2201 16 -0.2104204799953564 2201 17 -2.4441863450678767 2201 18 0.5090978638860548 2201 19 0.828715303176601 2201 20 0.683899754638432 2201 21 -0.23513649436296902 2201 22 -0.7378679539957105 2201 23 -1.0399651587983385 2201 24 -0.63058121952497 2201 25 -0.6070879707656829 2201 26 -0.787341509323582 2201 27 -1.2996072227879196 2201 28 -1.0064403053426787 2201 29 -0.8129309127757693 2201 30 -1.1321011870638173 2201 31 0.3501872407592259 2201 32 -0.38785462251107106 2201 33 -0.41957633776841163 2201 34 -0.13482944816993836 2201 35 -0.7287565021453446 2201 36 0.5953745072660794 2201 37 0.8978388089421685 2201 38 0.8828891790725564 2201 39 0.6740568434631911 2201 40 -0.11227564136368784 2201 41 -0.46306909959978526 2201 42 0.8448320482066478 2201 43 -0.011353024688787627 2201 44 0.3840493738663113 2201 45 -0.32852410883998573 2201 46 -0.5859513979070954 2201 47 0.13610782472511573 2201 48 0.27327546450985807 2201 49 0.5899654617068819 2201 50 0.18760776596960937 2201 51 -1.1423973945957115 1101 1 0.6151169932610827 1101 2 0.4969819319096871 1101 3 0.5955660305395369 1101 4 -0.5542572071440901 1101 5 0.5243888191232884 1101 6 0.24791253207269648 1101 7 0.580368213759165 1101 8 0.6648375804371156 1101 9 0.4067977184411973 1101 10 0.25822519307332925 1101 11 0.7312830328056208 1101 12 0.21855968194882536 1101 13 -0.6112740205260536 1101 14 0.7480803481130351 1101 15 0.8157027281787492 1101 16 0.6141715945967192 1101 17 0.12516097427943862 1101 18 -0.26770866292047657 1101 19 0.8543563288176301 1101 20 0.19264101337969208 1101 21 0.5265185172920397 1101 22 0.306421090293334 1101 23 -1.7614103802435608 1101 24 -0.3106511495949036 1101 25 0.26645515277743925 1101 26 0.4504207284386581 1101 27 0.508085084904387 1101 28 0.41080911190673675 1101 29 -1.0291313789762384 1101 30 -0.5429403479029773 1101 31 0.7557816463536255 1101 32 0.8522386175821666 1101 33 0.6194679512758796 1101 34 0.9286903653498726 1101 35 0.8108705374816978 1101 36 0.0860504979420682 1101 37 0.18571759682095745 1101 38 -0.635758839575459 1101 39 0.8949193143256622 1101 40 -0.23290501199305425 1101 41 0.258376121845437 1101 42 -0.11029615692155659 1101 43 0.2829360196002568 1101 44 0.32519156500850244 1101 45 -0.4334192137350854 1101 46 -0.6896810016366999 1101 47 0.03878847740576674 1101 48 -0.5967711555367643 1101 49 0.1424130141544353 1101 50 -0.22964165127980607 1101 51 -1.733889236087549 2202 1 0.6141068922509803 2202 2 -0.012614027686268514 2202 3 -0.4276662926927841 2202 4 -0.35223700512388234 2202 5 0.5456009403354116 2202 6 -0.1005723164121477 2202 7 0.2722874056783553 2202 8 -0.9548593892598518 2202 9 0.08104014268362647 2202 10 -0.28621925137111504 2202 11 -0.37780787628528856 2202 12 0.21401422740337495 2202 13 0.3367057774537443 2202 14 0.8445449945776815 2202 15 1.0455007079767284 2202 16 1.245989776414902 2202 17 0.8105145096329736 2202 18 0.4287559835441712 2202 19 0.14324521770652154 2202 20 0.3830450537837322 2202 21 0.1340942748677989 2202 22 -0.5036799198076736 2202 23 -0.3220164408496231 2202 24 0.6514700625263075 2202 25 0.08009151641380896 2202 26 -0.18392270590477722 2202 27 -0.5030260262067259 2202 28 0.6431323442299686 2202 29 0.6400605402156856 2202 30 0.5191808642182352 2202 31 0.586084676656661 2202 32 0.9027436680872185 2202 33 0.34623562804355856 2202 34 -0.24252175586224922 2202 35 0.3088503354614929 2202 36 0.6244343363259048 2202 37 0.16450547560883422 2202 38 0.2521199483033243 2202 39 0.8398688092751598 2202 40 -0.10714743623547918 2202 41 0.5523155157848336 2202 42 0.795259398634002 2202 43 0.7561683428325807 2202 44 0.4438784336953745 2202 45 0.43728785697198447 2202 46 0.6567836448279465 2202 47 0.12414201275930736 2202 48 0.039087430321828265 2202 49 -0.45960718786577104 2202 50 -0.9286315502697065 2202 51 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6 -0.6454441112839433 1215 7 0.21673185012279816 1215 8 -0.5676799020803642 1215 9 -0.19460088295740263 1215 10 0.20053288538102265 1215 11 0.12988443140702088 1215 12 -0.1398319264427812 1215 13 -0.6060292652813004 1215 14 0.20437405440674183 1215 15 0.310457972933996 1215 16 0.0459897764148991 1215 17 -0.7262376271191613 1215 18 -0.5614149566267699 1215 19 -0.7225667481054501 1215 20 -0.3737925530538746 1215 21 0.3481968389703667 1215 22 0.14033717420942082 1215 23 -0.2771446459778275 1215 24 0.6001880112442564 1215 25 0.9185530548753462 1215 26 0.5972738752918048 1215 27 0.2773158541351535 1215 28 0.3461237972214235 1215 29 0.31527421542936196 1215 30 0.5794372744746497 1215 31 0.5617257022976858 1215 32 0.5672735826171333 1215 33 0.5355518673597928 1215 34 0.49337568003518584 1215 35 -0.2415770149658556 1215 36 0.5633232252147948 1215 37 0.1927106038139641 1215 38 0.5880173842007608 1215 39 0.5330312024375488 1215 40 0.27233974325169896 1215 41 0.6202642337335504 1215 42 0.2358576892322901 1215 43 -0.22930174263750303 1215 44 -0.14159165177471067 1215 45 0.4407066603907879 1215 46 0.3627665508108535 1215 47 -0.10107166245437327 1215 48 -0.13698094574655073 1215 49 0.5130423847838017 1215 50 -0.08803325967141262 1215 51 0.46016670796839065 1212 1 -1.2303375521934612 1212 2 -0.5748362499084934 1212 3 -0.7698885149150101 1212 4 -0.2033481162349986 1212 5 -0.36106572633126177 1212 6 -0.7739056497454868 1212 7 -0.5032681498772007 1212 8 -0.6415260559265192 1212 9 0.08770680935029418 1212 10 -1.1217748069266733 1212 11 -1.0578078762852883 1212 12 -0.8459857725966273 1212 13 -0.1121831114351437 1212 14 -0.9710105609778736 1212 15 -1.5033881809121539 1212 16 -1.6140102235850975 1212 17 -0.2939299348114659 1212 18 -0.4667995720113822 1212 19 -2.5056436711823693 1212 20 -1.6791771684384855 1212 21 -0.6125723917988637 1212 22 -0.5281243642521218 1212 23 -1.2086831075162863 1212 24 -1.7351966041403557 1212 25 -0.3799084835861919 1212 26 -1.5550338170158895 1212 27 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12 -0.05495128983800868 1213 13 -0.43494173212480547 1213 14 0.17519633557385106 1213 15 0.5014394052947395 1213 16 -0.35056194772302973 1213 17 -0.7063437279149127 1213 18 -0.04748922718379589 1213 19 0.13021839778314614 1213 20 -0.4805564787833134 1213 21 0.07432415992526842 1213 22 -0.9757105711486744 1213 23 -0.5576486247576682 1213 24 -0.5841621213817376 1213 25 -0.4819774491034323 1213 26 -0.2674476101193335 1213 27 -0.2850183633714778 1213 28 -0.08292129561677797 1213 29 -0.4672191532709036 1213 30 -0.2302444231380889 1213 31 0.20562490654171484 1213 32 0.21117278686116236 1213 33 -0.5035728010752223 1213 34 0.5862138763216578 1213 35 0.1854786879519139 1213 36 0.2615990872837628 1213 37 -0.044689926690011816 1213 38 0.38775213221137506 1213 39 0.1245431387770708 1213 40 -0.2117451373849093 1213 41 -0.10055804743355878 1213 42 0.19142798100947545 1213 43 0.37149401333066834 1213 44 -0.36838210270309446 1213 45 0.3346058646348169 1213 46 0.7288142961689488 1213 47 -0.06857829375410773 1213 48 -0.6038244470728102 1213 49 -0.8795305595132774 1213 50 0.0054680665885342705 1213 51 0.10075026234505202 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 1.0 18 3.0 19 4.0 20 4.0 21 15.0 22 24.0 23 39.0 24 63.0 25 101.0 26 159.0 27 173.0 28 297.0 29 353.0 30 465.0 31 587.0 32 664.0 33 883.0 34 1086.0 35 1289.0 36 1736.0 37 2473.0 38 4507.0 39 15243.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.37530667727604 25.863669517936476 14.325973078708309 19.435050726079172 2 11.342393105734173 27.431223069274115 38.147166058999005 23.079217765992706 3 19.526019224395093 33.07590321511435 30.679482930062978 16.718594630427578 4 11.276102088167054 22.999668544912165 36.85780576731853 28.866423599602253 5 11.289360291680477 39.09844216108717 33.74875704342062 15.863440503811734 6 28.153795160755717 42.00198873052701 13.904540934703347 15.93967517401392 7 17.093138879681803 41.72356645674511 20.57673185283394 20.606562810739145 8 20.944647000331454 38.501823002983095 21.829632084852502 18.723897911832946 9 21.160092807424594 24.524361948955917 21.87935034802784 32.43619489559165 10 13.954259197878688 34.54424925422605 28.84322174345376 22.658269804441495 11 25.096128347918324 29.72686290108724 22.88517634579687 22.29183240519756 12 20.45409347033477 34.13655949618827 29.300629764666887 16.10871726881008 13 25.50878355982764 28.296320848525024 26.98707325157441 19.20782234007292 14 18.60457408021213 29.446470003314552 30.788863109048727 21.160092807424594 15 20.599933708982434 34.86576068942659 27.58369240967849 16.950613191912495 16 14.965197215777263 37.34835929731521 28.35598276433543 19.33046072257209 17 14.365263506794829 36.78157109711634 31.78985747431223 17.063307921776598 18 14.965197215777263 31.402055021544577 38.80013258203513 14.832615180643021 19 15.502154458070933 34.55419290686112 35.46237984753066 14.48127278753729 20 15.120981107059993 32.34338747099768 36.479946967185946 16.05568445475638 21 11.849519390122639 36.41365594961883 35.81703679151475 15.919787868743784 22 12.333443818362612 39.00232018561485 35.72422936692078 12.940006629101758 23 11.276102088167054 36.15512098110706 37.106397083195226 15.462379847530661 24 14.38183626118661 36.009280742459396 38.11402055021544 11.494862446138548 25 13.45707656612529 36.59927079880676 36.8445475638051 13.099105071262843 26 12.645011600928074 36.51309247596951 38.47530659595625 12.36658932714617 27 12.88034471329135 37.86542923433875 37.72621809744779 11.528007954922108 28 11.441829632084852 35.91978786874378 37.368246602585344 15.270135896586012 29 12.320185614849189 36.35399403380842 38.753728869738154 12.572091481604241 30 12.018561484918793 37.497514086841235 37.620152469340404 12.86377195889957 31 14.806098773616174 36.741796486576064 36.54623798475306 11.90586675505469 32 12.114683460391117 38.93934371892608 36.96387139542592 11.982101425256877 33 11.471660589990057 38.43221743453762 38.78355982764335 11.31256214782897 34 11.803115677825653 38.15048060987736 37.205833609545905 12.840570102751078 35 11.793172025190586 38.33278090818694 36.476632416307595 13.397414650314882 36 11.372224063639377 38.263175339741466 38.49187935034803 11.87272124627113 37 11.660589990056348 39.2243950944647 35.90321511435201 13.211799801126947 38 11.862777593636062 38.594630427577066 37.33510109380179 12.207490884985084 39 12.55883327809082 38.007954922108055 35.89658601259529 13.536625787205834 40 12.867086509777925 37.19588995691084 37.70964534305602 12.22737819025522 41 12.618495193901227 37.26218097447796 36.69870732515744 13.420616506463373 42 13.692409678488564 38.206827974809414 36.29433211799801 11.806430228704011 43 11.292674842558833 38.37255551872721 38.031156778256545 12.303612860457408 44 11.48491879350348 39.74146503148823 36.21146834603911 12.562147828969175 45 11.428571428571429 38.43553198541598 37.18594630427577 12.949950281736825 46 13.25820351342393 37.421279416639045 36.06231355651309 13.25820351342393 47 12.67152800795492 37.73284719920451 35.150812064965194 14.444812727875373 48 11.644017235664569 37.358302949950286 35.50215445807093 15.495525356314218 49 14.053695724229367 37.835598276433544 34.888962545575076 13.221743453762016 50 11.713622804110043 38.73715611534637 36.13523367583692 13.413987404706662 51 11.36228041100431 38.498508452104744 34.97845541929069 15.160755717600265 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1428.0 1 807.5 2 187.0 3 246.0 4 305.0 5 353.5 6 402.0 7 562.0 8 722.0 9 535.0 10 348.0 11 562.0 12 776.0 13 1403.5 14 2031.0 15 2058.0 16 2085.0 17 1548.5 18 1012.0 19 810.5 20 609.0 21 889.5 22 1170.0 23 774.5 24 379.0 25 442.5 26 831.5 27 1157.0 28 960.0 29 763.0 30 931.5 31 1100.0 32 1264.0 33 1428.0 34 1530.5 35 1633.0 36 1602.0 37 1571.0 38 1428.5 39 1286.0 40 1184.0 41 1082.0 42 1053.0 43 1024.0 44 1015.0 45 1006.0 46 973.5 47 941.0 48 962.0 49 983.0 50 1140.0 51 1297.0 52 1048.0 53 799.0 54 713.0 55 627.0 56 549.5 57 472.0 58 416.0 59 360.0 60 321.0 61 282.0 62 222.0 63 162.0 64 137.0 65 112.0 66 86.0 67 60.0 68 49.0 69 38.0 70 26.0 71 14.0 72 10.5 73 7.0 74 5.5 75 2.0 76 0.0 77 0.5 78 1.0 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026516407026847863 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.006629101756711966 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 30170.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.41059600200608 #Duplication Level Percentage of deduplicated Percentage of total 1 93.52980798671628 55.56661636444002 2 4.327051214067548 5.14145383117914 3 0.8799976398390104 1.5684355278958289 4 0.26873360182406425 0.6386249380053378 5 0.1544042137776624 0.45866231828760406 6 0.0692787753092729 0.24695359988477797 7 0.07954981384274501 0.330827129657228 8 0.07320270218183865 0.34792129324643084 9 0.03921362401879716 0.20967342969197877 >10 0.3562386769942753 4.836609043248156 >50 0.09457174435713042 3.9759600751407693 >100 0.10013478814283945 12.007201124676588 >500 0.01668913135713991 6.005253081072766 >1k 0.011126087571426607 8.665808243573359 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1360 4.507789194564136 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1262 4.18296320848525 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 746 2.4726549552535633 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 539 1.7865429234338748 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 532 1.7633410672853826 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 440 1.4584023864766325 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 337 1.1170036460059662 No Hit GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 292 0.967848856479947 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 281 0.9313887968180312 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 270 0.8949287371561153 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 231 0.765661252900232 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 213 0.7059993370898243 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 202 0.6695392774279085 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 185 0.6131919124958568 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 168 0.5568445475638051 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 162 0.5369572422936693 No Hit TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 133 0.44083526682134566 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 133 0.44083526682134566 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 130 0.4308916141862778 No Hit GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 126 0.41763341067285387 No Hit ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 124 0.41100430891614187 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 104 0.34471329134902223 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAA 102 0.33808418959231024 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA 98 0.3248259860788863 No Hit GTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 91 0.30162412993039445 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 90 0.2983095790520385 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 86 0.28505137553861454 No Hit GCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 85 0.2817368246602585 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 83 0.27510772290354657 No Hit CCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 76 0.25190586675505466 No Hit ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.2287040106065628 No Hit GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 67 0.22207490884985087 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 64 0.2121312562147829 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 60 0.19887305270135897 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 58 0.192243950944647 No Hit CTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 58 0.192243950944647 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 57 0.18892940006629103 No Hit TACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 56 0.18561484918793503 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 54 0.17898574743122309 No Hit CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 51 0.16904209479615512 No Hit GATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 49 0.16241299303944315 No Hit CCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.1557838912827312 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 45 0.14915478952601924 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAA 43 0.14252568776930727 No Hit TTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTCT 42 0.13921113689095127 RNA PCR Primer, Index 27 (95% over 21bp) GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40 0.1325820351342393 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 40 0.1325820351342393 No Hit TGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 38 0.12595293337752733 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAA 38 0.12595293337752733 No Hit GCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 36 0.11932383162081538 No Hit GTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAA 34 0.11269472986410341 No Hit TACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33 0.10938017898574742 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33 0.10938017898574742 No Hit ATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32 0.10606562810739145 No Hit ACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAA 31 0.10275107722903547 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.003314550878355983 16 0.0 0.0 0.003314550878355983 17 0.0 0.0 0.003314550878355983 18 0.0 0.0 0.003314550878355983 19 0.0 0.0 0.003314550878355983 20 0.0 0.0 0.003314550878355983 21 0.0 0.0 0.006629101756711966 22 0.0 0.0 0.013258203513423931 23 0.0 0.0 0.02320185614849188 24 0.0 0.0 0.033145508783559825 25 0.0 0.0 0.039774610540271794 26 0.0 0.0 0.0762346702021876 27 0.0 0.0 0.08286377195889957 28 0.0 0.0 0.11269472986410341 29 0.0 0.0 0.15909844216108718 30 0.0 0.0 0.20218760357971494 31 0.0 0.0 0.33808418959231024 32 0.0 0.0 0.45409347033476966 33 0.0 0.0 0.573417301955585 34 0.0 0.0 0.768975803778588 35 0.0 0.0 0.8849850845210474 36 0.0 0.0 1.0904872389791183 37 0.0 0.0 1.3258203513423932 38 0.0 0.0 1.5909844216108717 39 0.0 0.0 1.902552204176334 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 55 2.564775E-9 36.816826 45 AAAAGTA 80 0.0 36.561153 2 CTATCTC 50 4.616777E-8 35.998676 35 CCTATCT 45 8.334955E-7 34.998707 34 AGACTAG 45 8.334955E-7 34.998707 25 GACTAGA 45 8.334955E-7 34.998707 26 ATCTCGT 60 6.004484E-9 33.748756 37 TCCTATC 40 1.5113415E-5 33.748756 33 CCACGAG 60 6.004484E-9 33.748756 20 ACGAGAC 60 6.004484E-9 33.748756 22 TATCTCG 60 6.004484E-9 33.748756 36 GATCCTA 40 1.5113415E-5 33.748756 31 CGAGCCC 60 6.004484E-9 33.748756 15 CCGAGCC 60 6.004484E-9 33.748756 14 TCTCCGA 60 6.004484E-9 33.748756 11 ACTAGAT 40 1.5113415E-5 33.748756 27 TCCGAGC 60 6.004484E-9 33.748756 13 TAGATCC 40 1.5113415E-5 33.748756 29 CTCCGAG 60 6.004484E-9 33.748756 12 GTATGCC 55 1.06741936E-7 32.726067 42 >>END_MODULE