FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_16.34100000006911.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_16.34100000006911.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5660
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC111919.770318021201412TruSeq Adapter, Index 12 (95% over 21bp)
GCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1061.872791519434629No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGTC981.7314487632508833No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT891.5724381625441697TruSeq Adapter, Index 12 (95% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT871.5371024734982333No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAA791.3957597173144876No Hit
ACTCTGCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAAAA560.9893992932862191No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA540.9540636042402828No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAA440.7773851590106008No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA390.6890459363957597No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.6537102473498233No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.5653710247349824No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.5653710247349824No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC310.5477031802120141No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT290.5123674911660777No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA230.40636042402826855No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.3886925795053004No Hit
GCGCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT200.35335689045936397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.33568904593639576No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC190.33568904593639576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.33568904593639576No Hit
GTACTCTGCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAA180.3180212014134276No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC170.3003533568904594No Hit
GTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.3003533568904594No Hit
CCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.2826855123674912No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.22968197879858657No Hit
GCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT120.21201413427561835No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACT120.21201413427561835No Hit
GCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAGTA120.21201413427561835No Hit
GATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.21201413427561835No Hit
AAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAA120.21201413427561835No Hit
CTCTGCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAA110.1943462897526502No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAA110.1943462897526502No Hit
CTGCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAA110.1943462897526502No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC110.1943462897526502No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAA90.1590106007067138No Hit
GTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAA90.1590106007067138No Hit
CGGAGATGTGTATAAGAGACAGGCGTCAGATGTGTATAAGAGACAGCTTAT90.1590106007067138No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.1590106007067138No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTG90.1590106007067138No Hit
AAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAA80.1413427561837456No Hit
TTGCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT80.1413427561837456No Hit
AAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAA80.1413427561837456No Hit
TTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTCT70.12367491166077739Illumina PCR Primer Index 8 (95% over 21bp)
TACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.12367491166077739No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAGTACTCTGCGT70.12367491166077739No Hit
TGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA70.12367491166077739No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.12367491166077739No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAGTACTCTGCGTTGATA70.12367491166077739No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC70.12367491166077739RNA PCR Primer, Index 27 (95% over 21bp)
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAGTACTCTGCGTTGA60.10600706713780918No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC60.10600706713780918No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC60.10600706713780918No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACTCTGCGTTGAT60.10600706713780918No Hit
GCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAGTAC60.10600706713780918No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTT60.10600706713780918RNA PCR Primer, Index 12 (95% over 23bp)
TTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.10600706713780918No Hit
GCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAGT60.10600706713780918No Hit
CTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAA60.10600706713780918No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAA60.10600706713780918No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC60.10600706713780918TruSeq Adapter, Index 12 (95% over 24bp)
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAGTACT60.10600706713780918No Hit
GCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAG60.10600706713780918No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCTCG206.453524E-445.036
CTTATAC1950.043.8461531
TCTCGTA2000.042.7538
CTCGTAT2000.042.7539
GTATGCC1950.042.69230742
ATCTCGT1950.042.69230737
TGCCGTC1950.042.69230745
TATGCCG1950.042.69230743
ATGCCGT1950.042.69230744
CGTATGC1950.042.69230741
TCGTATG1950.042.69230740
AATCTCG1750.042.4285736
ACTTGCA1700.042.35294330
TTGCAAT1650.042.27272832
TGCAATC1650.042.27272833
CAATCTC1650.042.27272835
GCAATCT1650.042.27272834
AGACTAC1650.042.27272825
TACTTGC1650.042.27272829
CTACTTG1650.042.27272828
Produced by FastQC (version 0.11.2)