##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_15.34100000006904.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6422 Sequences flagged as poor quality 0 Sequence length 51 %GC 33 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.25910931174089 38.0 38.0 38.0 32.0 38.0 2 36.404079725942076 38.0 38.0 38.0 32.0 38.0 3 36.21364061040175 38.0 38.0 38.0 32.0 38.0 4 36.327000934288385 38.0 38.0 38.0 32.0 38.0 5 36.41030831516662 38.0 38.0 38.0 32.0 38.0 6 38.35066957334164 40.0 38.0 40.0 38.0 40.0 7 38.22999065711616 40.0 38.0 40.0 38.0 40.0 8 38.14574898785425 40.0 38.0 40.0 38.0 40.0 9 38.255216443475554 40.0 38.0 40.0 38.0 40.0 10 38.12083463095609 40.0 38.0 40.0 38.0 40.0 11 38.05107443164123 40.0 38.0 40.0 32.0 40.0 12 37.853939582684525 40.0 38.0 40.0 32.0 40.0 13 37.9112426035503 40.0 38.0 40.0 32.0 40.0 14 37.84584241669262 40.0 38.0 40.0 32.0 40.0 15 37.83042665836188 40.0 38.0 40.0 32.0 40.0 16 37.6186546247275 40.0 38.0 40.0 32.0 40.0 17 37.67751479289941 40.0 38.0 40.0 32.0 40.0 18 37.5288072251635 40.0 38.0 40.0 32.0 40.0 19 37.87651821862348 40.0 38.0 40.0 32.0 40.0 20 37.90750545001557 40.0 38.0 40.0 32.0 40.0 21 37.933821239489255 40.0 38.0 40.0 32.0 40.0 22 38.049517284335096 40.0 38.0 40.0 32.0 40.0 23 37.99283712239178 40.0 38.0 40.0 32.0 40.0 24 37.83198380566802 40.0 38.0 40.0 32.0 40.0 25 37.71940205543444 40.0 38.0 40.0 32.0 40.0 26 37.568981625661785 40.0 38.0 40.0 32.0 40.0 27 37.60557458735597 40.0 38.0 40.0 32.0 40.0 28 37.60791030831517 40.0 38.0 40.0 32.0 40.0 29 37.46278417938337 40.0 38.0 40.0 32.0 40.0 30 37.34288383681096 40.0 38.0 40.0 32.0 40.0 31 37.23263780753659 40.0 38.0 40.0 32.0 40.0 32 37.003425724073495 40.0 38.0 40.0 32.0 40.0 33 36.77405792587979 40.0 38.0 40.0 32.0 40.0 34 36.44565555901588 40.0 38.0 40.0 27.0 40.0 35 36.33011522890065 40.0 38.0 40.0 27.0 40.0 36 36.12597321706633 40.0 38.0 40.0 27.0 40.0 37 35.89317969479913 40.0 38.0 40.0 27.0 40.0 38 35.803955154157585 40.0 38.0 40.0 27.0 40.0 39 35.345219557770164 38.0 38.0 40.0 13.0 40.0 40 35.20336343818125 38.0 38.0 40.0 13.0 40.0 41 35.05372158206166 38.0 38.0 40.0 13.0 40.0 42 34.814388041108685 38.0 32.0 40.0 13.0 40.0 43 34.59514170040486 38.0 32.0 40.0 13.0 40.0 44 34.21457489878542 38.0 32.0 40.0 13.0 40.0 45 34.07567735907817 38.0 32.0 40.0 13.0 40.0 46 33.60261600747431 38.0 32.0 40.0 13.0 40.0 47 33.179227654936156 38.0 27.0 40.0 13.0 40.0 48 32.87776393646839 38.0 27.0 40.0 13.0 40.0 49 32.41544690127686 38.0 27.0 40.0 13.0 40.0 50 31.944876985362814 38.0 27.0 40.0 13.0 40.0 51 31.27841793833697 38.0 27.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 0.9982360521423033 2201 2 0.7892231900044493 2201 3 1.6615414099789163 2201 4 0.9085748452936002 2201 5 0.8906366636835372 2201 6 1.141162916944161 2201 7 1.0617127664002837 2201 8 1.1914835598429363 2201 9 0.8850225295537726 2201 10 1.2756357357919867 2201 11 1.4166326295232565 2201 12 1.9881754963786378 2201 13 1.1337085223804095 2201 14 1.5777433937590288 2201 15 -0.06501449938949833 2201 16 1.59683619332057 2201 17 0.07723170926296063 2201 18 -2.3178891854673154 2201 19 -2.07107918123544 2201 20 0.5397461913086943 2201 21 -1.75876211462149 2201 22 -2.6640621964840747 2201 23 -1.0054817578254927 2201 24 0.48099318216505793 2201 25 1.075194293553679 2201 26 1.7001387501387555 2201 27 1.902821484071481 2201 28 -0.40829617431180765 2201 29 1.787528747294374 2201 30 1.632291233072479 2201 31 1.869691332972586 2201 32 1.3435269157925305 2201 33 -1.3127994401431877 2201 34 -0.4268292905011677 2201 35 0.07322256736320298 2201 36 -0.09645211559274713 2201 37 0.3069319135725408 2201 38 0.16656178023364987 2201 39 1.450113991910868 2201 40 1.5187124073842782 2201 41 1.339641781829286 2201 42 1.2908282255938488 2201 43 3.117986787518035 2201 44 3.6774133852258757 2201 45 -0.8777823305167018 2201 46 2.2805936251248724 2201 47 0.15605583652457966 2201 48 2.518619618228996 2201 49 2.707184698981571 2201 50 1.7851139745670892 2201 51 -1.1605821695665348 1101 1 0.33156938547563186 1101 2 1.4558898566711136 1101 3 0.3282080766455806 1101 4 1.5752415119602645 1101 5 1.5573033303502015 1101 6 1.3633851391663825 1101 7 1.283934988622505 1101 8 1.4137057820651577 1101 9 1.3294669739982155 1101 10 1.4978579580142082 1101 11 1.194410407301035 1101 12 1.9881754963786378 1101 13 1.8003751890470738 1101 14 1.1332989493145789 1101 15 -1.2872367216117198 1101 16 2.263502859987234 1101 17 0.6327872648185178 1101 18 0.5709997034215775 1101 19 0.9289208187645599 1101 20 0.7619684135309157 1101 21 1.24123788537851 1101 22 1.3359378035159253 1101 23 1.327851575507843 1101 24 0.36988207105395077 1101 25 -1.0359168175574354 1101 26 0.47791652791653405 1101 27 0.45837703962703813 1101 28 1.2583704923548638 1101 29 2.231973191738817 1101 30 2.2989578997391504 1101 31 2.0919135551948074 1101 32 2.676860249125866 1101 33 3.020533893190148 1101 34 3.017615153943275 1101 35 3.073222567363203 1101 36 0.5702145510739172 1101 37 2.6402652469058694 1101 38 1.2776728913447641 1101 39 2.339002880799754 1101 40 -1.2590653703935004 1101 41 -1.215913773726271 1101 42 0.2908282255938488 1101 43 -3.770902101370851 1101 44 2.1218578296703186 1101 45 1.6777732250388482 1101 46 1.1694825140137581 1101 47 -0.2883886079198632 1101 48 3.518619618228996 1101 49 3.2627402545371282 1101 50 2.785113974567089 1101 51 3.0616400526556866 2202 1 -0.04938299547674774 2202 2 -0.2583958576146017 2202 3 0.8043985528360622 2202 4 0.7180986548174104 2202 5 -1.0141252410783679 2202 6 0.7284645042457498 2202 7 0.5855222902098021 2202 8 0.5565629249223036 2202 9 1.2659749105061522 2202 10 -2.4862690261127796 2202 11 -0.4246372117465853 2202 12 1.353254861458005 2202 13 -0.26311687444498943 2202 14 -4.041304225288592 2202 15 -4.065014499389498 2202 16 -0.37141777493339845 2202 17 0.29945393148518207 2202 18 0.8090949415168112 2202 19 1.2463811362248762 2202 20 1.3016509532134606 2202 21 0.8285394726800988 2202 22 1.1454616130397355 2202 23 -0.7991325514762835 2202 24 1.4651201662920457 2202 25 1.3926546110139952 2202 26 0.43029748029748305 2202 27 0.6329802142302086 2202 28 0.7186879526723189 2202 29 0.7399096996753229 2202 30 2.171973772755024 2202 31 1.3935008567821043 2202 32 1.7562253284909417 2202 33 2.0998989725552235 2202 34 2.604916741244864 2202 35 2.3748098689505 2202 36 1.363865344724708 2202 37 1.4021700088106357 2202 38 -0.7858191721472991 2202 39 -3.676870135073262 2202 40 1.963156851828721 2202 41 3.292022734210235 2202 42 -3.3282193934537716 2202 43 3.8005264700577186 2202 44 0.740905448717939 2202 45 3.074598621864247 2202 46 4.455196799728043 2202 47 5.743357423826168 2202 48 4.566238665848047 2202 49 6.691311683108559 2202 50 3.1978123872655004 2202 51 5.569576560592193 1102 1 0.9982360521423033 1102 2 0.7892231900044493 1102 3 0.3282080766455806 1102 4 1.5752415119602645 1102 5 0.8906366636835372 1102 6 1.141162916944161 1102 7 0.8394905441780622 1102 8 0.9692613376207149 1102 9 1.107244751775994 1102 10 1.4978579580142082 1102 11 0.7499659628565851 1102 12 1.7659532741564163 1102 13 1.355930744602631 1102 14 1.3555211715368074 1102 15 1.2683188339438374 1102 16 1.15239174887612 1102 17 0.9661205981518464 1102 18 1.348777481199356 1102 19 1.3733652632090028 1102 20 1.2064128579753657 1102 21 1.4634601076007314 1102 22 -1.2196177520396319 1102 23 0.8834071310634002 1102 24 0.48099318216505793 1102 25 1.2974165157759003 1102 26 -0.18875013875013735 1102 27 1.2361548174048096 1102 28 1.7028149367993066 1102 29 1.787528747294374 1102 30 1.632291233072479 1102 31 2.0919135551948074 1102 32 2.2324158046814233 1102 33 2.7983116709679265 1102 34 0.017615153943275175 1102 35 -0.5934440993034684 1102 36 0.1257701066294743 1102 37 -0.13751253087190918 1102 38 0.8332284469003213 1102 39 1.2278917696886467 1102 40 1.2964901851620567 1102 41 -2.3270248848373853 1102 42 -1.7091717744061512 1102 43 0.11798678751803493 1102 44 1.78852449633699 1102 45 0.6777732250388482 1102 46 -0.7194063748751276 1102 47 -0.06616638569764177 1102 48 1.5186196182289962 1102 49 0.5960735878704639 1102 50 1.0073361967893106 1102 51 1.6171956082112438 2203 1 1.0649027188089661 2203 2 0.25588985667111075 2203 3 0.46154140997891346 2203 4 -0.22475848803973264 2203 5 0.9573033303502001 2203 6 0.9856073613886025 2203 7 1.3283794330669494 2203 8 0.8137057820651563 2203 9 1.351689196220434 2203 10 1.5423024024586525 2203 11 1.4610770739677008 2203 12 1.8103977186008606 2203 13 0.6225974112692967 2203 14 1.444410060425696 2203 15 0.9349855006105017 2203 16 -0.41427491779054293 2203 17 1.0994539314851792 2203 18 1.6376663700882403 2203 19 1.417809707653447 2203 20 1.4730795246420314 2203 21 1.48568232982295 2203 22 1.602604470182591 2203 23 1.3722960199522873 2203 24 1.236548737720618 2203 25 1.1640831824425675 2203 26 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3.212059598387718 2204 35 3.017667011807646 2204 36 3.0424367732961386 2204 37 3.473598580239205 2204 38 4.249895113566986 2204 39 3.394558436355311 2204 40 0.08815685182872102 2204 41 1.1313084484959504 2204 42 1.332494892260513 2204 43 1.6040978986291492 2204 44 3.3301911630036543 2204 45 2.1638843361499625 2204 46 2.294482514013758 2204 47 2.2255002809690225 2204 48 3.226952951562332 2204 49 3.6377402545371282 2204 50 2.9656695301226463 2204 51 2.533862274877908 2205 1 -1.0017639478576967 2205 2 -0.5441101433288864 2205 3 -0.3384585900210837 2205 4 -0.4247584880397355 2205 5 0.22396999701686582 2205 6 0.9189406947219396 2205 7 -1.716065011377495 2205 8 0.08037244873182203 2205 9 0.6628003073315512 2205 10 -2.3910309308746847 2205 11 -0.9167007038100792 2205 12 -2.345157836954698 2205 13 0.6892640779359596 2205 14 -2.311145495129864 2205 15 0.37942994505494454 2205 16 -0.2920526955683229 2205 17 1.1883428203740678 2205 18 1.348777481199356 2205 19 1.1511430409867813 2205 20 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1215 12 -1.5396022813991408 1215 13 -0.31073592206404044 1215 14 0.7444100604256931 1215 15 0.4349855006105017 1215 16 -0.9309415844572086 1215 17 -0.9505460685148179 1215 18 0.5709997034215775 1215 19 -0.9321902923465544 1215 20 -2.2102538086913057 1215 21 -1.3143176701770471 1215 22 -2.2473955298174104 1215 23 -2.727703980047714 1215 24 -2.1301179289460492 1215 25 -1.119250150890771 1215 26 -0.43875013875013735 1215 27 -1.6527340714840761 1215 28 -0.9360739520895862 1215 29 0.45419541396103824 1215 30 1.2434023441835933 1215 31 -0.8803086670274141 1215 32 0.3157491380147519 1215 33 0.4094227820790337 1215 34 -0.45460706827894626 1215 35 -1.7323329881923542 1215 36 -1.3742298933705257 1215 37 -0.3597347530941306 1215 38 -0.7501048864330144 1215 39 1.4778917696886467 1215 40 -0.7035098148379433 1215 41 -1.2436915515040496 1215 42 -1.042505107739487 1215 43 0.6457645652958135 1215 44 -0.6281421703296814 1215 45 1.3722176694832982 1215 46 -0.7471841526529062 1215 47 -1.8161663856976453 1215 48 0.01861961822899616 1215 49 0.012740254537128237 1215 50 2.0073361967893106 1215 51 1.3671956082112438 1212 1 0.6649027188089676 1212 2 -1.0441101433288864 1212 3 -1.9217919233544194 1212 4 -0.4247584880397355 1212 5 -1.3593633363164628 1212 6 0.4189406947219396 1212 7 0.22837943306694797 1212 8 0.580372448731822 1212 9 -1.3649774704462274 1212 10 -0.30769759754134896 1212 11 -1.555589592698965 1212 12 0.3770643852675235 1212 13 -0.9774025887307047 1212 14 -1.5055899395743069 1212 15 -2.898347832722834 1212 16 -1.2642749177905444 1212 17 -3.0338794018481536 1212 18 -2.0123336299117582 1212 19 -0.34885695901321867 1212 20 0.37307952464203 1212 21 -0.48098433684371145 1212 22 -4.664062196484075 1212 23 -1.8110373133810498 1212 24 -1.0467845956127206 1212 25 -0.5359168175574354 1212 26 -1.772083472083466 1212 27 -2.0694007381507404 1212 28 -2.3527406187562505 1212 29 -2.6291379193722975 1212 30 -0.173264322483071 1212 31 -1.880308667027414 1212 32 0.1490824713480876 1212 33 1.5760894487457051 1212 34 -0.45460706827894626 1212 35 -0.9823329881923542 1212 36 0.7924367732961386 1212 37 2.806931913572541 1212 38 -0.5834382197663501 1212 39 -1.605441563644689 1212 40 1.7964901851620567 1212 41 0.8396417818292861 1212 42 2.290828225593849 1212 43 1.812431231962485 1212 44 2.78852449633699 1212 45 3.2055510028166267 1212 46 3.5861491806804224 1212 47 3.1838336143023582 1212 48 2.018619618228996 1212 49 1.7627402545371282 1212 50 2.3406695301226463 1212 51 -0.6328043917887598 1213 1 -0.9065258526196018 1213 2 -0.2583958576146017 1213 3 -0.4813157328782296 1213 4 -0.5676156308968814 1213 5 -0.6569823839355138 1213 6 -0.05724978146854198 1213 7 1.1569508616383786 1213 8 -1.8005799322205576 1213 9 0.6945463390775828 1213 10 -1.414840454684203 1213 11 -0.8532086403180159 1213 12 -1.9324594242562867 1213 13 0.7368831255550106 1213 14 -0.6841613681457375 1213 15 -3.5650144993894983 1213 16 -0.5142749177905444 1213 17 -3.057688925657672 1213 18 -1.9051907727689041 1213 19 -1.5393331494894085 1213 20 -2.1983490467865394 1213 21 -0.02860338446276245 1213 22 0.7168901844683049 1213 23 0.3437245913808553 1213 24 -1.6063084051365308 1213 25 -0.17877396041458127 1213 26 1.0017260517260524 1213 27 0.49012307137306976 1213 28 -1.8527406187562505 1213 29 -2.402947443181816 1213 30 -0.8280262272449761 1213 31 0.32207228535353494 1213 32 1.613368185633803 1213 33 -0.11438674173048469 1213 34 0.8906310269591486 1213 35 1.8033812975219305 1213 36 1.1495796304389927 1213 37 0.7593128659534898 1213 38 0.571323684995555 1213 39 1.894558436355311 1213 40 1.463156851828721 1213 41 1.7205941627816657 1213 42 -2.399647964882341 1213 43 -0.9851878156565661 1213 44 -1.1876659798534916 1213 45 -0.49682994956432225 1213 46 -1.9733746288433842 1213 47 0.8147859952547378 1213 48 -0.6480470484376681 1213 49 -1.8086883168914447 1213 50 1.0549552444083616 1213 51 1.0695765605921927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 1.0 19 1.0 20 2.0 21 1.0 22 7.0 23 12.0 24 34.0 25 27.0 26 71.0 27 94.0 28 97.0 29 146.0 30 188.0 31 237.0 32 268.0 33 316.0 34 346.0 35 383.0 36 461.0 37 555.0 38 881.0 39 2293.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.378563639196138 21.59214830970556 15.04907306434024 32.980214986758064 2 6.041731547804422 24.727499221426346 51.837433821239486 17.39333540952974 3 19.604484584241668 31.158517595764557 43.257552164434756 5.979445655559016 4 9.467455621301776 42.26097788850826 29.41451261289318 18.857053877296792 5 6.057303020865774 29.88165680473373 48.70756773590782 15.353472438492682 6 22.127063220180627 51.712862036748675 9.638741824976643 16.52133291809405 7 5.309872313920897 35.76767362192464 19.697913422609776 39.22454064154469 8 13.749610713173466 44.05169729056369 19.573341638118965 22.62535035814388 9 14.388041108688881 23.37278106508876 18.670196200560575 43.56898162566179 10 10.137028962939894 42.79040797259421 23.637496107131735 23.435066957334165 11 13.500467144191841 20.959202740579258 37.76082217377764 27.77950794145126 12 14.901899719713485 23.248209280597944 27.85736530675802 33.992525692930556 13 17.37776393646839 15.820616630333229 44.70569915914046 22.095920274057924 14 15.83618810339458 18.249766427904078 25.490501401432574 40.423544067268764 15 22.858922454064153 15.898473995639986 24.229212083463096 37.013391466832765 16 31.516661476175646 30.255372158206168 19.33976954219869 18.888196823419495 17 15.493615696044847 47.5241357832451 24.05792587978823 12.924322640921831 18 32.37309249454998 22.376206789162254 33.213952039862974 12.03674867642479 19 11.055745873559639 30.084085954531297 25.72407349735285 33.13609467455622 20 10.915602616007474 21.192774836499535 29.13422609778885 38.75739644970414 21 3.955154157583307 32.74680784802243 28.34008097165992 34.95795702273435 22 4.998442852693865 54.20429772656493 28.417938336966674 12.379321083774524 23 6.5711616318903765 28.371223917782622 31.34537527250078 33.71223917782622 24 27.795079414512614 35.56524447212706 30.87823108066023 5.7614450327000934 25 10.697601993148552 51.65057614450327 28.947368421052634 8.704453441295547 26 27.265649330426655 33.15166614761756 32.77795079414513 6.804733727810651 27 7.194020554344441 54.07972594207412 31.34537527250078 7.38087823108066 28 3.768296480847088 31.00280286515104 33.50981002802865 31.719090625973216 29 5.465587044534413 32.57552164434755 54.967299906571164 6.99159140454687 30 5.123812490266314 51.03566422675596 35.29045320043607 8.550070082541659 31 7.412021177203363 30.67580193086266 33.5565244472127 28.35565244472127 32 5.652444721270633 30.39551541575833 55.4344440984117 8.517595764559328 33 7.754593584553099 30.831516661476176 54.39115540330115 7.022734350669574 34 27.047648707567735 32.15509187169106 31.142946122703208 9.654313298037994 35 6.820305200872003 34.66209903456867 29.41451261289318 29.103083151666148 36 5.8237309249455 55.917159763313606 30.86265960759888 7.396449704142012 37 4.282155091871691 57.100591715976336 30.76923076923077 7.848022422921208 38 5.091871691061974 33.758953597010276 51.37028962939895 9.778885082528808 39 6.02616007474307 31.734662099034566 31.407661164746187 30.831516661476176 40 5.4967299906571165 31.93709124883214 51.46371846776705 11.102460292743693 41 8.377452507007163 31.610090314543754 28.791653690439116 31.220803488009967 42 29.476798505138586 32.24852071005917 30.348800996574276 7.925879788227966 43 7.427592650264716 33.82123948925568 50.17128620367487 8.579881656804734 44 6.5711616318903765 55.18530052943008 30.17751479289941 8.066023045780131 45 8.797882279663655 31.594518841482405 49.797570850202426 9.81002802865151 46 30.55123014637185 30.411086888819682 26.518218623481783 12.519464341326689 47 8.268452195577702 29.22765493615696 26.378075365929615 36.12581750233572 48 10.168171909062597 28.044222983494237 25.365929616941763 36.42167549050141 49 31.205232014948614 27.904079725942072 28.013080037371534 12.877608221737777 50 9.327312363749611 26.954219869199626 48.48956711304889 15.228900654001867 51 8.502024291497975 26.08221737776394 28.215509187169108 37.20024914356898 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 86.0 1 53.0 2 20.0 3 22.5 4 25.0 5 30.5 6 36.0 7 34.0 8 32.0 9 42.5 10 53.0 11 81.5 12 110.0 13 158.5 14 207.0 15 313.5 16 420.0 17 321.5 18 223.0 19 215.0 20 207.0 21 286.0 22 365.0 23 320.0 24 275.0 25 247.0 26 281.5 27 344.0 28 334.0 29 324.0 30 288.5 31 253.0 32 266.5 33 280.0 34 300.0 35 320.0 36 268.0 37 216.0 38 184.0 39 152.0 40 131.5 41 111.0 42 86.0 43 61.0 44 60.5 45 60.0 46 63.5 47 67.0 48 192.0 49 317.0 50 911.5 51 1506.0 52 782.5 53 59.0 54 41.0 55 23.0 56 16.5 57 10.0 58 8.0 59 6.0 60 8.0 61 10.0 62 9.0 63 8.0 64 7.0 65 6.0 66 5.5 67 5.0 68 3.5 69 2.0 70 2.0 71 2.0 72 1.0 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.5 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04671441918405481 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.015571473061351605 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 6422.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.39426969791342 #Duplication Level Percentage of deduplicated Percentage of total 1 88.39003858295335 39.24011211460604 2 5.752367590319187 5.1074431641233256 3 1.4380918975797967 1.9152911865462474 4 1.2977902490354263 2.3045780130800373 5 0.526131182041389 1.1678604796013703 6 0.4209049456331112 1.1211460604173156 7 0.28060329708874077 0.8720024914356898 8 0.1052262364082778 0.3737153534724385 9 0.035075412136092596 0.14014325755216445 >10 1.3679410733076114 12.581750233572095 >50 0.28060329708874077 9.436312675179073 >100 0.07015082427218519 5.138586110246029 >500 0.0 0.0 >1k 0.035075412136092596 20.601058860168173 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC 1323 20.601058860168173 TruSeq Adapter, Index 12 (95% over 21bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 170 2.6471504204297727 No Hit TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT 160 2.4914356898162566 TruSeq Adapter, Index 12 (95% over 22bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 82 1.2768607910308314 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 80 1.2457178449081283 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 79 1.2301463718467767 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 77 1.1990034257240736 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 76 1.183431952662722 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 74 1.1522890065400186 No Hit TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT 73 1.136717533478667 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 65 1.0121457489878543 No Hit CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 45 0.7007162877608222 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43 0.669573341638119 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42 0.6540018685767673 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 41 0.6384303955154157 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 38 0.591715976331361 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 32 0.49828713796325136 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG 26 0.4048582995951417 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26 0.4048582995951417 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25 0.3892868265337901 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.35814388041108686 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 23 0.35814388041108686 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA 22 0.3425724073497353 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA 22 0.3425724073497353 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 22 0.3425724073497353 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21 0.32700093428838367 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 21 0.32700093428838367 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA 20 0.3114294612270321 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20 0.3114294612270321 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20 0.3114294612270321 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.2802865151043289 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 18 0.2802865151043289 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 18 0.2802865151043289 No Hit ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.24914356898162568 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.24914356898162568 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.24914356898162568 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAA 15 0.23357209592027406 No Hit GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.21800062285892244 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14 0.21800062285892244 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA 13 0.20242914979757085 No Hit ATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.20242914979757085 No Hit GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.20242914979757085 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.20242914979757085 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.20242914979757085 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC 12 0.18685767673621925 No Hit CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 12 0.18685767673621925 No Hit CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 11 0.17128620367486766 No Hit CTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.17128620367486766 No Hit GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10 0.15571473061351604 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC 10 0.15571473061351604 TruSeq Adapter, Index 12 (95% over 24bp) CTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.14014325755216445 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAA 8 0.12457178449081284 No Hit CTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8 0.12457178449081284 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTCTGTCTCTTATACACATC 8 0.12457178449081284 No Hit TTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.10900031142946122 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.10900031142946122 No Hit TCCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 7 0.10900031142946122 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.10900031142946122 No Hit GTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.10900031142946122 No Hit GTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCCGAGCC 7 0.10900031142946122 No Hit TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.10900031142946122 No Hit ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.10900031142946122 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.015571473061351603 8 0.0 0.0 0.015571473061351603 9 0.0 0.0 0.015571473061351603 10 0.0 0.0 0.015571473061351603 11 0.0 0.0 0.015571473061351603 12 0.0 0.0 0.015571473061351603 13 0.0 0.0 0.015571473061351603 14 0.0 0.0 0.015571473061351603 15 0.0 0.0 0.015571473061351603 16 0.0 0.0 0.015571473061351603 17 0.0 0.0 0.031142946122703206 18 0.0 0.0 0.04671441918405481 19 0.0 0.0 0.04671441918405481 20 0.0 0.0 0.04671441918405481 21 0.0 0.0 0.04671441918405481 22 0.0 0.0 0.07785736530675802 23 0.0 0.0 0.23357209592027406 24 0.0 0.0 0.2958579881656805 25 0.0 0.0 0.3114294612270321 26 0.0 0.0 0.5450015571473061 27 0.0 0.0 0.7785736530675802 28 0.0 0.0 1.1211460604173156 29 0.0 0.0 1.7907194020554345 30 0.0 0.0 2.164434755527873 31 0.0 0.0 3.145437558393024 32 0.0 0.0 3.9862971037060104 33 0.0 0.0 4.827156649018997 34 0.0 0.0 5.683587667393335 35 0.0 0.0 6.088445966988477 36 0.0 0.0 6.60230457801308 37 0.0 0.0 7.131734662099035 38 0.0 0.0 7.676736219246341 39 0.0 0.0 8.34630956088446 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 165 0.0 39.545452 45 ATGCCGT 170 0.0 38.38235 44 AATCTCG 160 0.0 37.96875 36 CGAGACT 160 0.0 37.96875 23 TTGCAAT 155 0.0 37.741936 32 TGCAATC 155 0.0 37.741936 33 ACTTGCA 155 0.0 37.741936 30 CAATCTC 155 0.0 37.741936 35 GCAATCT 155 0.0 37.741936 34 AGACTAC 155 0.0 37.741936 25 TACTTGC 155 0.0 37.741936 29 CTACTTG 155 0.0 37.741936 28 GAGACTA 155 0.0 37.741936 24 ACTACTT 155 0.0 37.741936 27 GACTACT 155 0.0 37.741936 26 GTATGCC 175 0.0 37.285713 42 ATCTCGT 175 0.0 37.285713 37 GCCCACG 175 0.0 37.285713 18 CCACGAG 175 0.0 37.285713 20 TATGCCG 175 0.0 37.285713 43 >>END_MODULE