##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_14.341000000068f6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11495 Sequences flagged as poor quality 0 Sequence length 51 %GC 24 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.34606350587212 38.0 38.0 38.0 32.0 38.0 2 36.24732492387995 38.0 38.0 38.0 32.0 38.0 3 36.27986080904741 38.0 38.0 38.0 32.0 38.0 4 36.2029578077425 38.0 38.0 38.0 32.0 38.0 5 36.252544584602 38.0 38.0 38.0 32.0 38.0 6 38.21383210091344 40.0 38.0 40.0 38.0 40.0 7 38.09421487603306 40.0 38.0 40.0 32.0 40.0 8 38.09447585906916 40.0 38.0 40.0 38.0 40.0 9 38.093344932579384 40.0 38.0 40.0 38.0 40.0 10 37.98086124401914 40.0 38.0 40.0 32.0 40.0 11 37.79504132231405 40.0 38.0 40.0 32.0 40.0 12 37.76520226185298 40.0 38.0 40.0 32.0 40.0 13 37.680034797738145 40.0 38.0 40.0 32.0 40.0 14 37.664810787298826 40.0 38.0 40.0 32.0 40.0 15 37.57503262287951 40.0 38.0 40.0 32.0 40.0 16 37.59530230535015 40.0 38.0 40.0 32.0 40.0 17 37.66437581557199 40.0 38.0 40.0 32.0 40.0 18 37.56929099608526 40.0 38.0 40.0 32.0 40.0 19 37.81217920835146 40.0 38.0 40.0 32.0 40.0 20 37.84680295780774 40.0 38.0 40.0 32.0 40.0 21 37.95476294040888 40.0 38.0 40.0 32.0 40.0 22 37.98929969551979 40.0 38.0 40.0 32.0 40.0 23 37.95667681600696 40.0 38.0 40.0 32.0 40.0 24 37.901087429317094 40.0 38.0 40.0 32.0 40.0 25 37.81948673336233 40.0 38.0 40.0 32.0 40.0 26 37.72605480643758 40.0 38.0 40.0 32.0 40.0 27 37.719530230535014 40.0 38.0 40.0 32.0 40.0 28 37.62322749021314 40.0 38.0 40.0 32.0 40.0 29 37.52448890822097 40.0 38.0 40.0 32.0 40.0 30 37.48307959982601 40.0 38.0 40.0 32.0 40.0 31 37.48829926054806 40.0 38.0 40.0 32.0 40.0 32 37.44393214441061 40.0 38.0 40.0 32.0 40.0 33 37.25028273162244 40.0 38.0 40.0 32.0 40.0 34 37.123444976076556 40.0 38.0 40.0 32.0 40.0 35 36.97842540234885 40.0 38.0 40.0 32.0 40.0 36 36.81644193127447 40.0 38.0 40.0 32.0 40.0 37 36.6882122662027 40.0 38.0 40.0 27.0 40.0 38 36.46846454980426 40.0 38.0 40.0 27.0 40.0 39 36.14858634188778 40.0 38.0 40.0 27.0 40.0 40 36.035754675946066 40.0 38.0 40.0 27.0 40.0 41 35.74780339277947 40.0 38.0 40.0 27.0 40.0 42 35.567377120487166 40.0 38.0 40.0 13.0 40.0 43 35.329360591561546 40.0 38.0 40.0 13.0 40.0 44 35.00461070030448 40.0 38.0 40.0 13.0 40.0 45 34.76581122227055 40.0 38.0 40.0 13.0 40.0 46 34.43993040452371 38.0 32.0 40.0 13.0 40.0 47 34.1608525445846 38.0 32.0 40.0 13.0 40.0 48 33.87194432361896 38.0 32.0 40.0 13.0 40.0 49 33.57799043062201 38.0 32.0 40.0 13.0 40.0 50 33.179904306220095 38.0 27.0 40.0 13.0 40.0 51 32.884123531970424 38.0 27.0 40.0 13.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.8800098493273794 2201 2 0.25357093554409005 2201 3 -0.9102107684686516 2201 4 1.2330382414207364 2201 5 0.40223500276069757 2201 6 -1.1616570090656566 2201 7 -1.9363320208983126 2201 8 -1.0281727798436364 2201 9 -0.06136902714925441 2201 10 0.9151520068598202 2201 11 0.3657718821454239 2201 12 -0.3812781040294624 2201 13 1.0657066283978196 2201 14 0.30327535470429723 2201 15 0.3500526741729715 2201 16 0.636017131471597 2201 17 1.2189476991823796 2201 18 0.04845996250164575 2201 19 -0.3576488922493155 2201 20 0.1324618522407448 2201 21 0.6159833625665172 2201 22 0.45316615120471937 2201 23 0.5311598555707704 2201 24 0.9453938834979425 2201 25 -0.3508708471923683 2201 26 0.01005065093809776 2201 27 1.1044346809668397 2201 28 1.174550519614833 2201 29 0.9701132105471828 2201 30 1.0387654166008318 2201 31 0.16644865320572677 2201 32 0.45752689287700576 2201 33 1.6188503416375681 2201 34 1.3053802077973202 2201 35 1.876314042265392 2201 36 1.1213767887997292 2201 37 2.3292956145039483 2201 38 1.4350817030257446 2201 39 2.141514113813784 2201 40 2.6563053513179185 2201 41 3.613104471760323 2201 42 3.2207885308762982 2201 43 2.2266027885629853 2201 44 1.2678622711312073 2201 45 3.6114821903956127 2201 46 2.6724377049645085 2201 47 1.5941054252971298 2201 48 1.5754771926303022 2201 49 1.9437891331970363 2201 50 2.2386752676687465 2201 51 2.3440689543211164 1101 1 0.4300098493273765 1101 2 0.8910709355440929 1101 3 1.3772892315313499 1101 4 1.5205382414207378 1101 5 1.1022350027607004 1101 6 0.7091763242676734 1101 7 1.467834645768356 1101 8 1.1718272201563664 1101 9 0.8094643061840756 1101 10 1.498485340193156 1101 11 1.228271882145421 1101 12 0.09788856263720191 1101 13 0.7157066283978182 1101 14 -0.1717246452957042 1101 15 -0.7291139924936942 1101 16 0.577683798138267 1101 17 0.5439476991823824 1101 18 1.15262662916831 1101 19 1.1381844410840145 1101 20 1.5282951855740805 1101 21 1.9034833625665186 1101 22 0.8156661512047165 1101 23 1.301993188904106 1101 24 -0.07543944983539319 1101 25 -1.3967041805257026 1101 26 0.5975506509380963 1101 27 -0.7705653190331603 1101 28 0.9037171862814972 1101 29 1.011779877213847 1101 30 -0.7237345833991711 1101 31 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1.7410027556573269 1208 39 -1.253222728291476 1208 40 1.3997264039495008 1208 41 0.5736307875497957 1208 42 0.5431569519289283 1208 43 -0.1253708956475421 1208 44 -0.5643745709740529 1208 45 -1.6615441253938599 1208 46 -4.93611492661444 1208 47 -3.0177366799660277 1208 48 -2.0528122810539067 1208 49 -2.0430529720661212 1208 50 -0.6429036796996712 1208 51 -4.0835626246262535 1209 1 0.20143842075594875 1209 2 0.07202331649646965 1209 3 0.6344320886742025 1209 4 -0.41279509191259933 1209 5 -0.09776499723930243 1209 6 1.109176324267679 1209 7 1.1440251219588333 1209 8 -1.0281727798436364 1209 9 0.6380357347555048 1209 10 0.5461043878121998 1209 11 0.4282718821454239 1209 12 0.3359838007324427 1209 13 -0.3033409906498008 1209 14 -0.47648655005760787 1209 15 0.3470764836967817 1209 16 1.2729218933763562 1209 17 0.15347150870618975 1209 18 -0.9426114660697849 1209 19 -1.0332441303445563 1209 20 0.956866614145504 1209 21 1.1606262197093713 1209 22 0.8251899607285296 1209 23 1.301993188904106 1209 24 -0.8849632593592034 1209 25 0.47948629566477763 1209 26 1.1499316033190468 1209 27 0.7056251571573142 1209 28 0.9037171862814972 1209 29 1.249875115309088 1209 30 1.142932083267496 1209 31 1.5265677008247778 1209 32 1.7908602262103415 1209 33 1.1426598654470936 1209 34 1.4422849697020794 1209 35 1.2572664232177715 1209 36 0.47256726499020374 1209 37 -0.19451390930557722 1209 38 0.8963912268352701 1209 39 1.879609351909025 1209 40 2.459876779889349 1209 41 3.220247328903177 1209 42 -0.7375448024570375 1209 43 1.1402932647534598 1209 44 2.806552747321682 1209 45 1.4061250475384668 1209 46 3.4373186573454575 1209 47 2.623867330059035 1209 48 2.8344057640588716 1209 49 2.074741514149416 1209 50 2.369627648621126 1209 51 -0.3642643790122193 1214 1 -0.8245356052180739 1214 2 0.8728891173622699 1214 3 -1.2227107684686516 1214 4 -0.8067344858519903 1214 5 1.0840531845788774 1214 6 0.5940248091161635 1214 7 -0.2261047481710392 1214 8 -0.8463545980254565 1214 9 0.5124946092143787 1214 10 0.8924247341325469 1214 11 1.064635518509057 1214 12 0.9766764414250844 1214 13 0.36116117385236635 1214 14 -1.0717246452957028 1214 15 0.5375526741729715 1214 16 0.8291989496534171 1214 17 0.4803113355460127 1214 18 0.6223235988652789 1214 19 1.113942016841591 1214 20 0.5585982158771117 1214 21 -0.6965166374334828 1214 22 0.3338479693865395 1214 23 0.7868416737525905 1214 24 -0.7421061165020575 1214 25 -0.20882539264691502 1214 26 -1.2569948036073555 1214 27 0.18398013551229297 1214 28 0.3279596105239264 1214 29 -0.4730686076346373 1214 30 0.430810871146285 1214 31 0.34258501684209364 1214 32 -0.042473107122994236 1214 33 -2.335695112907885 1214 34 -1.0468925194754064 1214 35 -1.5327768668255146 1214 36 0.04183133425427599 1214 37 0.4202047054130418 1214 38 -1.4001455697015288 1214 39 -0.31303134073166916 1214 40 -0.46869464868208155 1214 41 -1.0005318918760437 1214 42 2.823061258149025 1214 43 2.4368300612902587 1214 44 0.23945318022211382 1214 45 2.503527644941066 1214 46 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0.9551391293962013 1215 32 0.07657451192462617 1215 33 1.2855170083042324 1215 34 0.6803802077973202 1215 35 -0.7427335767822285 1215 36 -0.5274327350097963 1215 37 -3.7659424807341466 1215 38 -1.8655135350694962 1215 39 -2.9775335052338363 1215 40 -1.444885124872556 1215 41 -0.017847909192056477 1215 42 -0.07087813579036606 1215 43 0.14029326475345982 1215 44 -0.8124948717259386 1215 45 0.9775536189670433 1215 46 0.8182710382978442 1215 47 -0.13803743184572426 1215 48 -1.546546616893508 1215 49 -0.6395442001362994 1215 50 -1.0113247323312535 1215 51 1.1595451447973062 1212 1 0.8474011536752073 1212 2 0.08237528337017608 1212 3 0.7338109706617857 1212 4 0.8770599805511736 1212 5 0.33701761145634634 1212 6 0.5584516865865155 1212 7 -1.2162233252461405 1212 8 -0.9412162581045038 1212 9 -0.16734728801881715 1212 10 -0.7478914714010472 1212 11 -0.8760759439415295 1212 12 -0.3079085388120717 1212 13 -1.9886411976891338 1212 14 0.1239275286173438 1212 15 -0.3754908040878959 1212 16 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-1.2699901506726192 1213 2 -1.0089290644559128 1213 3 -0.322710768468653 1213 4 0.12053824142073921 1213 5 0.10223500276070041 1213 6 1.175842990934342 1213 7 1.2011679791016903 1213 8 0.4718272201563636 1213 9 -0.6738690271492516 1213 10 -1.3348479931401798 1213 11 -3.9717281178545747 1213 12 -2.468778104029461 1213 13 -2.7342933716021776 1213 14 -1.6717246452957042 1213 15 -1.31244732582703 1213 16 -0.28898286852840016 1213 17 -2.6060523008176233 1213 18 -0.7140400374983571 1213 19 -1.395148892249317 1213 20 -1.2050381477592538 1213 21 0.10348336256651436 1213 22 0.4656661512047222 1213 23 -0.9813401444292325 1213 24 -0.04210611650205465 1213 25 0.43662915280763315 1213 26 -0.8524493490618994 1213 27 -0.22056531903316312 1213 28 1.237050519614833 1213 29 0.8451132105471828 1213 30 -1.8737345833991697 1213 31 -1.3210513467942704 1213 32 -1.4424731071229928 1213 33 0.2688503416375667 1213 34 -0.21961979220267835 1213 35 0.1263140422653919 1213 36 -1.1536232112002693 1213 37 -0.020704385496053135 1213 38 1.472581703025746 1213 39 -0.5084858861862145 1213 40 1.431305351317917 1213 41 -0.43689552823967404 1213 42 0.7457885308762968 1213 43 -2.0358972114370175 1213 44 -1.5196377288687941 1213 45 -0.30101780960438873 1213 46 -0.4150622950354901 1213 47 -1.6308945747028716 1213 48 -0.18702280736970067 1213 49 -0.4562108668029623 1213 50 -0.5113247323312535 1213 51 -0.9309310456788822 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 2.0 18 1.0 19 2.0 20 2.0 21 7.0 22 10.0 23 23.0 24 35.0 25 67.0 26 90.0 27 136.0 28 179.0 29 227.0 30 293.0 31 374.0 32 433.0 33 510.0 34 554.0 35 672.0 36 741.0 37 969.0 38 1518.0 39 4650.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.245278092088085 26.399164418139087 14.431195056140655 20.924362433632172 2 8.08247781451192 29.60675134852967 39.342265529841654 22.968505307116757 3 24.245324053936493 36.163549369291 32.648977816441935 6.9421487603305785 4 10.430622009569378 32.144410613310136 32.144410613310136 25.280556763810353 5 7.481513701609395 34.92822966507177 39.79121357111788 17.799043062200955 6 27.612005219660723 44.288821226620264 9.926054806437582 18.17311874728143 7 6.715963462374945 43.07959982601131 21.20922140060896 28.995215311004785 8 18.068725532840364 35.87646802957808 24.00173988690735 22.053066550674206 9 16.589821661591998 27.629404088734233 22.627229230100042 33.15354501957373 10 12.144410613310134 36.67681600695955 23.879947803392778 27.29882557633754 11 16.311439756415833 27.785993910395824 25.324053936494128 30.57851239669421 12 17.250978686385384 34.29317094388865 27.464114832535884 20.99173553719008 13 21.75728577642453 23.906046107003046 33.11004784688995 21.22662026968247 14 17.1900826446281 26.559373640713353 30.88299260548064 25.367551109177906 15 24.462809917355372 24.697694649847758 27.759895606785555 23.07959982601131 16 16.459330143540672 37.63375380600261 24.088734232274902 21.818181818181817 17 12.144410613310134 42.40974336668116 31.239669421487605 14.206176598521097 18 17.443883765442838 28.68453105968331 41.064903427875414 12.806681746998432 19 10.12614180078295 36.81600695954763 34.8064375815572 18.251413658112224 20 10.648107872988257 29.047411918225315 37.41626794258373 22.888212266202697 21 3.3405828621139624 39.04306220095693 36.80730752501088 20.809047411918225 22 4.984775989560679 48.168769030013046 37.49456285341453 9.351892127011745 23 4.5498042627229225 36.13745106568073 40.47846889952153 18.834275772074815 24 13.745106568073076 41.4528055676381 40.31317964332318 4.488908220965637 25 7.09873858199217 46.2200956937799 38.88647237929534 7.794693344932579 26 12.266202696824706 39.53023053501523 41.93997390169639 6.2635928664636795 27 5.793823401478903 48.786428882122664 40.90474119182253 4.515006524575902 28 2.8534145280556764 38.72988255763376 42.52283601565898 15.893866898651588 29 3.758155719878208 40.20008699434537 51.69204001739887 4.349717268377556 30 4.140930839495432 47.872988255763374 42.45324053936494 5.53284036537625 31 4.541104828186168 39.46933449325794 41.09612875163114 14.89343192692475 32 4.5933014354066986 40.24358416702915 50.12614180078295 5.036972596781209 33 4.523705959112657 41.51370160939539 49.1083079599826 4.854284471509352 34 12.805567638103524 42.34884732492388 39.61722488038277 5.2283601565898215 35 4.088734232274902 43.41887777294476 39.21705089169204 13.2753371030883 36 3.5928664636798606 52.648977816441935 39.42583732057416 4.332318399304045 37 3.714658547194433 52.17920835145715 39.91300565463245 4.193127446715963 38 3.9756415832970857 42.41844280121792 47.716398434101784 5.88951718138321 39 4.062635928664637 42.41844280121792 39.70421922575033 13.814702044367117 40 4.506307090039147 42.32274902131362 47.063940843845145 6.107003044802088 41 5.654632448890822 42.10526315789473 37.88603740756851 14.354066985645932 42 13.997390169638974 41.86167899086559 38.59939103958243 5.541539799913005 43 5.297955632883863 42.77511961722488 46.01130926489778 5.9156154849934754 44 5.471944323618965 50.917790343627665 37.73814702044367 5.8721183123097 45 6.489778164419313 41.87907785993911 45.13266637668551 6.498477598956068 46 15.528490648107873 41.32231404958678 35.45019573727708 7.698999565028274 47 6.376685515441497 40.92214006089604 34.96302740321879 17.73814702044367 48 7.307525010874293 40.61765985210961 34.84993475424098 17.22488038277512 49 16.024358416702913 40.6002609830361 35.80687255328404 7.568508046976946 50 7.081339712918661 39.54762940408874 44.41931274467159 8.95171813832101 51 7.47281426707264 39.95650282731623 34.6324488908221 17.93823401478904 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 538.0 1 335.0 2 132.0 3 132.5 4 133.0 5 158.0 6 183.0 7 157.0 8 131.0 9 156.0 10 181.0 11 315.0 12 449.0 13 654.0 14 859.0 15 1090.0 16 1321.0 17 998.0 18 675.0 19 628.0 20 581.0 21 680.0 22 779.0 23 730.5 24 682.0 25 563.5 26 577.0 27 709.0 28 622.0 29 535.0 30 413.0 31 291.0 32 275.5 33 260.0 34 244.5 35 229.0 36 206.5 37 184.0 38 160.0 39 136.0 40 135.5 41 135.0 42 115.0 43 95.0 44 97.0 45 99.0 46 93.5 47 88.0 48 182.0 49 276.0 50 718.5 51 1161.0 52 623.5 53 86.0 54 64.5 55 43.0 56 34.0 57 25.0 58 23.5 59 22.0 60 15.0 61 8.0 62 7.5 63 7.0 64 4.5 65 2.0 66 5.0 67 8.0 68 4.5 69 1.0 70 1.5 71 2.0 72 2.0 73 2.0 74 1.0 75 0.0 76 0.0 77 0.5 78 1.0 79 0.5 80 0.0 81 0.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05219660722053066 2 0.008699434536755112 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.008699434536755112 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 11495.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.98956067855589 #Duplication Level Percentage of deduplicated Percentage of total 1 90.74208363548465 32.657677250978686 2 4.060913705583756 2.923010004349717 3 1.4986705342035291 1.6180948238364505 4 0.5076142131979695 0.7307525010874293 5 0.5076142131979695 0.9134406263592866 6 0.21754894851341552 0.469769464984776 7 0.21754894851341552 0.548064375815572 8 0.1208605269518975 0.34797738147020446 9 0.048344210780759005 0.156589821661592 >10 1.305293691080493 8.899521531100477 >50 0.41092579163645154 11.004784688995215 >100 0.290065264684554 21.17442366246194 >500 0.048344210780759005 9.830361026533275 >1k 0.024172105390379502 8.725532840365375 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC 1003 8.725532840365375 TruSeq Adapter, Index 12 (95% over 21bp) GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 623 5.419747716398434 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 507 4.410613310134841 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 327 2.8447150935189214 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 298 2.592431491953023 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 283 2.4619399739016963 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 268 2.33144845585037 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 245 2.1313614615050023 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 232 2.0182688125271855 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 195 1.6963897346672467 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 150 1.3049151805132666 No Hit TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT 128 1.1135276207046543 TruSeq Adapter, Index 12 (95% over 22bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 103 0.8960417572857765 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA 103 0.8960417572857765 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 102 0.8873423227490213 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 95 0.8264462809917356 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 93 0.8090474119182253 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 91 0.791648542844715 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 89 0.7742496737712048 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.7307525010874293 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.7307525010874293 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 78 0.6785558938668987 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 77 0.6698564593301436 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 77 0.6698564593301436 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 76 0.6611570247933884 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA 67 0.5828621139625925 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66 0.5741626794258373 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 64 0.5567638103523271 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 58 0.5045672031317964 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 58 0.5045672031317964 No Hit GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 55 0.4784688995215311 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 53 0.4610700304480209 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 50 0.4349717268377556 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 45 0.39147455415397997 No Hit ATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 45 0.39147455415397997 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 40 0.34797738147020446 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33 0.28708133971291866 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32 0.27838190517616357 No Hit CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32 0.27838190517616357 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 29 0.2522836015658982 No Hit GTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAA 27 0.234884732492388 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26 0.22618529795563289 No Hit CTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 26 0.22618529795563289 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25 0.2174858634188778 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC 22 0.19138755980861244 TruSeq Adapter, Index 12 (95% over 24bp) AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22 0.19138755980861244 No Hit TTTTTTTTCCGCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22 0.19138755980861244 No Hit TACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21 0.18268812527185732 No Hit CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20 0.17398869073510223 No Hit GTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAA 19 0.1652892561983471 No Hit TTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.1652892561983471 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19 0.1652892561983471 No Hit TTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.156589821661592 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 18 0.156589821661592 No Hit TATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTT 18 0.156589821661592 RNA PCR Primer, Index 12 (95% over 23bp) CTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17 0.14789038712483688 No Hit CTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17 0.14789038712483688 No Hit AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAA 16 0.13919095258808178 No Hit TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT 16 0.13919095258808178 No Hit GCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.13919095258808178 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.13049151805132667 No Hit TTTTTTTTTCCGCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.13049151805132667 No Hit GCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 15 0.13049151805132667 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.13049151805132667 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC 15 0.13049151805132667 No Hit CGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.13049151805132667 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.12179208351457155 No Hit AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAA 14 0.12179208351457155 No Hit CTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.11309264897781644 No Hit GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.11309264897781644 No Hit ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.10439321444106132 No Hit CGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.10439321444106132 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAA 12 0.10439321444106132 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.008699434536755112 2 0.0 0.0 0.008699434536755112 3 0.0 0.0 0.008699434536755112 4 0.0 0.0 0.008699434536755112 5 0.0 0.0 0.017398869073510223 6 0.0 0.0 0.017398869073510223 7 0.0 0.0 0.017398869073510223 8 0.0 0.0 0.017398869073510223 9 0.0 0.0 0.017398869073510223 10 0.0 0.0 0.017398869073510223 11 0.0 0.0 0.017398869073510223 12 0.0 0.0 0.017398869073510223 13 0.0 0.0 0.017398869073510223 14 0.0 0.0 0.017398869073510223 15 0.0 0.0 0.017398869073510223 16 0.0 0.0 0.017398869073510223 17 0.0 0.0 0.017398869073510223 18 0.0 0.0 0.02609830361026533 19 0.0 0.0 0.02609830361026533 20 0.0 0.0 0.02609830361026533 21 0.0 0.0 0.060896041757285774 22 0.0 0.0 0.06959547629404089 23 0.0 0.0 0.11309264897781644 24 0.0 0.0 0.156589821661592 25 0.0 0.0 0.156589821661592 26 0.0 0.0 0.234884732492388 27 0.0 0.0 0.2696824706394084 28 0.0 0.0 0.4001739886907351 29 0.0 0.0 0.5393649412788168 30 0.0 0.0 0.6176598521096128 31 0.0 0.0 1.0352327098738583 32 0.0 0.0 1.3571117877337973 33 0.0 0.0 1.5571987820791648 34 0.0 0.0 1.9051761635493694 35 0.0 0.0 2.1052631578947367 36 0.0 0.0 2.4010439321444106 37 0.0 0.0 2.662026968247064 38 0.0 0.0 3.053501522401044 39 0.0 0.0 3.392779469334493 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCCGTC 125 0.0 41.399998 45 ATGCCGT 130 0.0 39.80769 44 GTATGCC 135 0.0 38.333336 42 ATCTCGT 135 0.0 38.333336 37 CCACGAG 135 0.0 38.333336 20 TATGCCG 135 0.0 38.333336 43 CACGAGA 135 0.0 38.333336 21 CCCACGA 135 0.0 38.333336 19 TCTCGTA 135 0.0 38.333336 38 ACGAGAC 135 0.0 38.333336 22 CGTATGC 135 0.0 38.333336 41 TCGTATG 135 0.0 38.333336 40 CGAGACT 135 0.0 38.333336 23 CTCGTAT 135 0.0 38.333336 39 GAGACTA 135 0.0 38.333336 24 TTGCAAT 125 0.0 37.8 32 AATCTCG 125 0.0 37.8 36 TGCAATC 125 0.0 37.8 33 ACTTGCA 125 0.0 37.8 30 CAATCTC 125 0.0 37.8 35 >>END_MODULE