FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_14.341000000068f6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_14.341000000068f6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11495
Sequences flagged as poor quality0
Sequence length51
%GC24

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC10038.725532840365375TruSeq Adapter, Index 12 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6235.419747716398434No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5074.410613310134841No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3272.8447150935189214No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2982.592431491953023No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2832.4619399739016963No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2682.33144845585037No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2452.1313614615050023No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2322.0182688125271855No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1951.6963897346672467No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1501.3049151805132666No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT1281.1135276207046543TruSeq Adapter, Index 12 (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.8960417572857765No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA1030.8960417572857765No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.8873423227490213No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT950.8264462809917356No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.8090474119182253No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA910.791648542844715No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA890.7742496737712048No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.7307525010874293No Hit
ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA840.7307525010874293No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.6785558938668987No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.6698564593301436No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA770.6698564593301436No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.6611570247933884No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA670.5828621139625925No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA660.5741626794258373No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT640.5567638103523271No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA580.5045672031317964No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT580.5045672031317964No Hit
GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA550.4784688995215311No Hit
GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.4610700304480209No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.4349717268377556No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.39147455415397997No Hit
ATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.39147455415397997No Hit
GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.34797738147020446No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330.28708133971291866No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.27838190517616357No Hit
CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.27838190517616357No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.2522836015658982No Hit
GTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAA270.234884732492388No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.22618529795563289No Hit
CTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.22618529795563289No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.2174858634188778No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC220.19138755980861244TruSeq Adapter, Index 12 (95% over 24bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.19138755980861244No Hit
TTTTTTTTCCGCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.19138755980861244No Hit
TACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.18268812527185732No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.17398869073510223No Hit
GTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAA190.1652892561983471No Hit
TTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.1652892561983471No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.1652892561983471No Hit
TTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.156589821661592No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC180.156589821661592No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTT180.156589821661592RNA PCR Primer, Index 12 (95% over 23bp)
CTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.14789038712483688No Hit
CTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.14789038712483688No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAA160.13919095258808178No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT160.13919095258808178No Hit
GCGTTGATACCACTGCTTCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.13919095258808178No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.13049151805132667No Hit
TTTTTTTTTCCGCGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.13049151805132667No Hit
GCGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT150.13049151805132667No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.13049151805132667No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACACATCTCC150.13049151805132667No Hit
CGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.13049151805132667No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.12179208351457155No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAA140.12179208351457155No Hit
CTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAA130.11309264897781644No Hit
GCGTTGATACCACTGCTTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.11309264897781644No Hit
ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA120.10439321444106132No Hit
CGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10439321444106132No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAA120.10439321444106132No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1250.041.39999845
ATGCCGT1300.039.8076944
GTATGCC1350.038.33333642
ATCTCGT1350.038.33333637
CCACGAG1350.038.33333620
TATGCCG1350.038.33333643
CACGAGA1350.038.33333621
CCCACGA1350.038.33333619
TCTCGTA1350.038.33333638
ACGAGAC1350.038.33333622
CGTATGC1350.038.33333641
TCGTATG1350.038.33333640
CGAGACT1350.038.33333623
CTCGTAT1350.038.33333639
GAGACTA1350.038.33333624
TTGCAAT1250.037.832
AATCTCG1250.037.836
TGCAATC1250.037.833
ACTTGCA1250.037.830
CAATCTC1250.037.835
Produced by FastQC (version 0.11.2)