##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_11.341000000068cf.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8120 Sequences flagged as poor quality 0 Sequence length 51 %GC 25 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.338546798029554 38.0 38.0 38.0 32.0 38.0 2 36.32820197044335 38.0 38.0 38.0 32.0 38.0 3 36.279802955665026 38.0 38.0 38.0 32.0 38.0 4 36.33337438423645 38.0 38.0 38.0 32.0 38.0 5 36.453448275862065 38.0 38.0 38.0 32.0 38.0 6 38.395566502463055 40.0 38.0 40.0 38.0 40.0 7 38.19408866995074 40.0 38.0 40.0 38.0 40.0 8 38.22980295566502 40.0 38.0 40.0 38.0 40.0 9 38.2314039408867 40.0 38.0 40.0 38.0 40.0 10 38.09605911330049 40.0 38.0 40.0 38.0 40.0 11 38.07475369458128 40.0 38.0 40.0 32.0 40.0 12 37.87426108374384 40.0 38.0 40.0 32.0 40.0 13 37.89568965517241 40.0 38.0 40.0 32.0 40.0 14 37.74224137931034 40.0 38.0 40.0 32.0 40.0 15 37.8564039408867 40.0 38.0 40.0 32.0 40.0 16 37.798152709359606 40.0 38.0 40.0 32.0 40.0 17 37.75763546798029 40.0 38.0 40.0 32.0 40.0 18 37.79593596059113 40.0 38.0 40.0 32.0 40.0 19 37.99236453201971 40.0 38.0 40.0 32.0 40.0 20 38.00110837438424 40.0 38.0 40.0 32.0 40.0 21 38.0551724137931 40.0 38.0 40.0 32.0 40.0 22 38.123029556650245 40.0 38.0 40.0 32.0 40.0 23 38.08916256157635 40.0 38.0 40.0 32.0 40.0 24 38.07229064039409 40.0 38.0 40.0 32.0 40.0 25 38.01145320197044 40.0 38.0 40.0 32.0 40.0 26 37.88054187192118 40.0 38.0 40.0 32.0 40.0 27 37.81453201970444 40.0 38.0 40.0 32.0 40.0 28 37.811206896551724 40.0 38.0 40.0 32.0 40.0 29 37.733374384236456 40.0 38.0 40.0 32.0 40.0 30 37.71637931034483 40.0 38.0 40.0 32.0 40.0 31 37.83152709359606 40.0 38.0 40.0 32.0 40.0 32 37.77019704433498 40.0 38.0 40.0 32.0 40.0 33 37.724137931034484 40.0 38.0 40.0 32.0 40.0 34 37.55443349753695 40.0 38.0 40.0 32.0 40.0 35 37.5429802955665 40.0 38.0 40.0 32.0 40.0 36 37.479679802955665 40.0 38.0 40.0 32.0 40.0 37 37.539162561576354 40.0 38.0 40.0 32.0 40.0 38 37.50024630541872 40.0 38.0 40.0 32.0 40.0 39 37.325246305418716 40.0 38.0 40.0 32.0 40.0 40 37.18583743842365 40.0 38.0 40.0 32.0 40.0 41 37.11564039408867 40.0 38.0 40.0 32.0 40.0 42 37.10320197044335 40.0 38.0 40.0 32.0 40.0 43 37.075492610837436 40.0 38.0 40.0 32.0 40.0 44 36.927093596059116 40.0 38.0 40.0 32.0 40.0 45 36.880172413793105 40.0 38.0 40.0 32.0 40.0 46 36.6429802955665 40.0 38.0 40.0 27.0 40.0 47 36.551600985221675 40.0 38.0 40.0 27.0 40.0 48 36.289408866995075 40.0 38.0 40.0 27.0 40.0 49 36.19371921182266 40.0 38.0 40.0 27.0 40.0 50 36.03165024630542 40.0 38.0 40.0 27.0 40.0 51 35.989532019704434 40.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.8947511689699184 2201 2 0.30714656437312016 2201 3 -0.4279969813172855 2201 4 0.6341043071511905 2201 5 0.2804344743797884 2201 6 -0.21641494356337887 2201 7 0.2733020885364752 2201 8 1.1466076024669718 2201 9 0.19591077325451778 2201 10 1.3916004351551337 2201 11 0.12648731476856057 2201 12 0.600028031863971 2201 13 0.2631810897435827 2201 14 0.17685630341880199 2201 15 1.1226027661574562 2201 16 1.0779882660741862 2201 17 1.4234125683344558 2201 18 1.922110246524305 2201 19 1.7837719788891633 2201 20 0.6279302945318648 2201 21 0.6642922398781579 2201 22 0.3639033058954766 2201 23 1.416541596424402 2201 24 0.97467007818571 2201 25 1.6245816900113752 2201 26 -0.589134411595353 2201 27 0.995255959318456 2201 28 0.3958856898309904 2201 29 0.45583309919246773 2201 30 1.648008827110388 2201 31 1.6997292204323386 2201 32 1.2297488969364068 2201 33 1.2836100965007091 2201 34 1.6736099837662337 2201 35 -0.13829287032412907 2201 36 1.1932680167055167 2201 37 1.0856016596250981 2201 38 1.8997676802364438 2201 39 1.5190642907439909 2201 40 0.8723272387335044 2201 41 -0.46676925505051514 2201 42 0.07390372214590712 2201 43 1.0861853207556322 2201 44 0.7372821839618737 2201 45 0.8734279609279767 2201 46 1.4223146428224496 2201 47 0.07573294760795335 2201 48 2.6405548661408176 2201 49 3.07477611364331 2201 50 3.0719034654581563 2201 51 2.971927746732419 1101 1 -1.3375720633533135 1101 2 0.7313889886155494 1101 3 -2.4481990015193063 1101 4 0.3916800647269483 1101 5 0.7046768986222176 1101 6 0.75328202613359 1101 7 0.8389586541930427 1101 8 1.2476177034770757 1101 9 1.2060117833555282 1101 10 -0.17405613050143387 1101 11 1.055780244061495 1101 12 0.2767957086316457 1101 13 -2.6055057789432894 1101 14 -3.267588141025641 1101 15 -1.8975992540445645 1101 16 -1.0533248652389418 1101 17 0.5951297400516253 1101 18 -1.3809200565059925 1101 19 0.6322568273740075 1101 20 1.2339909005924738 1101 21 0.8663124418983656 1101 22 0.3538022957944662 1101 23 0.6690668489496545 1101 24 -0.11623901272338344 1101 25 0.43266249809218493 1101 26 0.7138958914349445 1101 27 0.9750539391164352 1101 28 1.0120473059926027 1101 29 1.2033078466672151 1101 30 0.4156855947871563 1101 31 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1.440205714424465 1102 2 0.8980556552822136 1102 3 1.662912109591808 1102 4 0.725013398060284 1102 5 0.8713435652888819 1102 6 0.9199486928002543 1102 7 1.0611808764152642 1102 8 1.1920621479215185 1102 9 0.650456227799971 1102 10 1.4370549806096804 1102 11 -1.3053308670496193 1102 12 0.5545734864094243 1102 13 -1.5777280011655108 1102 14 0.3435229700854663 1102 15 1.2135118570665497 1102 16 -0.7477693096833846 1102 17 0.9840186289405182 1102 18 -0.4642533898393282 1102 19 0.21559016070734316 1102 20 0.15065756725913815 1102 21 0.7552013307872514 1102 22 1.0760245180166876 1102 23 1.446844626727433 1102 24 1.5504276539432809 1102 25 1.8215513869810778 1102 26 1.8805625581016159 1102 27 1.5861650502275495 1102 28 -0.4601749162296187 1102 29 0.25886340222276516 1102 30 -0.7232032941017295 1102 31 0.8966989174020412 1102 32 1.290354957542469 1102 33 1.6927010055916156 1102 34 1.7190645292207805 1102 35 0.5511010690698086 1102 36 1.617510440947946 1102 37 2.0098440838675273 1102 38 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19 -0.7677431726259911 1214 20 -0.16600909940753183 1214 21 0.15520133078724996 1214 22 0.8426911846833534 1214 23 -1.2198220399392312 1214 24 -0.7162390127233849 1214 25 -1.4784486130189265 1214 26 -0.9527707752317198 1214 27 0.8861650502275467 1214 28 -0.2101749162296187 1214 29 1.1588634022227708 1214 30 1.6601300392316034 1214 31 -0.5366344159312959 1214 32 0.6236882908757977 1214 33 0.9927010055916128 1214 34 1.5523978625541162 1214 35 0.8011010690698086 1214 36 1.484177107614613 1214 37 1.2431774172008616 1214 38 1.7300707105394793 1214 39 -1.8385114668317684 1214 40 0.7995999660062338 1214 41 0.4665640782828149 1214 42 1.0617825100246918 1214 43 1.4740641086344155 1214 44 1.2706155172952052 1214 45 1.4491855366855475 1214 46 2.040496461004267 1214 47 1.475732947607952 1214 48 2.2526760782620343 1214 49 1.7535639924311894 1214 50 1.9325095260642158 1214 51 1.8325338073384785 1215 1 0.2259200001387498 1215 2 -0.8162300590035017 1215 3 -0.9799450332653379 1215 4 0.08215625520313807 1215 5 -1.628656434711118 1215 6 0.13423440708596956 1215 7 -0.5578667426323562 1215 8 -0.09365213779276615 1215 9 -1.4209723436285984 1215 10 0.2941978377525345 1215 11 0.658954847236096 1215 12 -0.4454265135905757 1215 13 0.6008434274059198 1215 14 0.7482848748473714 1215 15 -0.42934528579059617 1215 16 0.9546116426975644 1215 17 0.007828152750036566 1215 18 1.2857466101606718 1215 19 0.8227330178501973 1215 20 0.9006575672591381 1215 21 0.7552013307872514 1215 22 1.0522149942071692 1215 23 0.2801779600607688 1215 24 -1.544810441294814 1215 25 -0.6546390892094038 1215 26 -0.5956279180888657 1215 27 -0.3424063783438811 1215 28 -0.376841582896283 1215 29 0.8064824498418162 1215 30 -0.7351080560064958 1215 31 -0.8652058445027251 1215 32 0.4570216242091334 1215 33 -0.021584708694099675 1215 34 0.0047788149350651565 1215 35 -0.2227084547397098 1215 36 0.21274853618604084 1215 37 0.8907964648199069 1215 38 0.6205469010156648 1215 39 -0.07184480016510264 1215 40 1.0281713945776616 1215 41 1.4618021735209155 1215 42 0.957020605262791 1215 43 0.8978736324439396 1215 44 -1.9055749588952722 1215 45 -0.1460525585525474 1215 46 -0.3309321104242997 1215 47 0.5757329476079533 1215 48 -0.028276302690350974 1215 49 1.4059449448121413 1215 50 -0.4865380929834018 1215 51 -1.1579423831377085 1212 1 0.9786672528860052 1212 2 -0.37117511394855285 1212 3 0.7398351865148882 1212 4 0.3403980134448972 1212 5 -1.3978872039418846 1212 6 0.49687176972333447 1212 7 1.1124629276973153 1212 8 -0.15409169823232816 1212 9 0.11199468933843093 1212 10 0.3985934421481403 1212 11 0.8677460560273005 1212 12 0.8238042556401908 1212 13 0.9415027680652557 1212 14 -0.6692975427350447 1212 15 -0.40187275831806346 1212 16 -0.13879495070902692 1212 17 -0.7723916274697373 1212 18 -4.560407235993175 1212 19 -1.7267175316003502 1212 20 -1.5608808942793218 1212 21 -1.8601832845973618 1212 22 -0.6291036871115168 1212 23 -1.6813605014776911 1212 24 0.11453021804585006 1212 25 0.14206420749389537 1212 26 0.047229224768280176 1212 27 -0.2215272574647571 1212 28 -1.0306877367424363 1212 29 0.7075813509407141 1212 30 0.5011556802572414 1212 31 -0.705865185162061 1212 32 0.5724062395937466 1212 33 0.8080856209762288 1212 34 -1.3963200861638327 1212 35 -1.7886425206737755 1212 36 -0.17736135392384966 1212 37 -0.28502771100426827 1212 38 -0.3519805715118025 1212 39 -0.41799864631894934 1212 40 -1.1696308032245355 1212 41 -0.6975384858197486 1212 42 1.1438337920759736 1212 43 0.4638076983780053 1212 44 0.19882064550033363 1212 45 0.3209804084804233 1212 46 1.2404964610042697 1212 47 0.6141944860694935 1212 48 1.6475478731338242 1212 49 1.4663845052517033 1212 50 2.876099269653963 1212 51 2.468431243235912 1213 1 -0.15979428557553632 1213 2 -0.20194434471778777 1213 3 0.0629121095918066 1213 4 -0.341653268606386 1213 5 -0.16198976804444953 1213 6 0.21994869280025142 1213 7 -1.6721524569180701 1213 8 0.09206214792151712 1213 9 -0.8162104388666975 1213 10 0.0037216472763503816 1213 11 0.8780024662837107 1213 12 0.45457348640942286 1213 13 0.9722719988344863 1213 14 -1.3564770299145295 1213 15 -1.1864881429334488 1213 16 -1.0311026430167232 1213 17 0.15068529560718247 1213 18 0.15241327682733896 1213 19 0.7655901607073403 1213 20 0.4339909005924696 1213 21 -0.9781320025460829 1213 22 0.4426911846833548 1213 23 0.8468446267274388 1213 24 -0.3162390127233863 1213 25 0.38821805364774065 1213 26 0.047229224768280176 1213 27 1.1528317168942195 1213 28 -0.4768415828962844 1213 29 1.0255300688894309 1213 30 -0.2732032941017337 1213 31 -0.0033010825979644665 1213 32 0.7570216242091306 1213 33 0.45936767225828135 1213 34 -0.2476021374458881 1213 35 0.40110106906981 1213 36 1.0841771076146074 1213 37 1.776510750534193 1213 38 1.0634040438728078 1213 39 1.2281551998348945 1213 40 1.2662666326728953 1213 41 0.9998974116161534 1213 42 0.8617825100246961 1213 43 0.20739744196774978 1213 44 0.8039488506285437 1213 45 1.982518870018886 1213 46 2.4404964610042725 1213 47 2.275732947607956 1213 48 1.7860094115953657 1213 49 3.086897325764525 1213 50 3.1325095260642186 1213 51 3.0325338073384813 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 2.0 17 0.0 18 0.0 19 1.0 20 3.0 21 4.0 22 3.0 23 7.0 24 16.0 25 37.0 26 44.0 27 62.0 28 99.0 29 110.0 30 148.0 31 167.0 32 199.0 33 271.0 34 300.0 35 323.0 36 475.0 37 690.0 38 1237.0 39 3922.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.228135008622814 26.435082532643506 13.697955161369796 23.638827297363882 2 7.586206896551724 27.46305418719212 42.64778325123152 22.302955665024633 3 19.655172413793103 35.65270935960591 37.426108374384235 7.266009852216748 4 9.285714285714286 35.80049261083744 30.8743842364532 24.039408866995075 5 7.758620689655173 33.953201970443345 41.05911330049261 17.22906403940887 6 24.10098522167488 48.977832512315274 9.926108374384237 16.995073891625616 7 7.352216748768472 44.08866995073892 16.945812807881776 31.613300492610836 8 16.564039408866996 42.0935960591133 21.945812807881772 19.396551724137932 9 15.775862068965518 28.57142857142857 20.20935960591133 35.443349753694584 10 10.788177339901479 43.65763546798029 22.70935960591133 22.844827586206897 11 15.529556650246306 28.3128078817734 30.110837438423644 26.04679802955665 12 15.443349753694582 34.80295566502463 26.157635467980295 23.596059113300495 13 19.445812807881772 26.29310344827586 36.79802955665025 17.463054187192117 14 15.320197044334977 28.485221674876847 28.97783251231527 27.216748768472904 15 20.899014778325125 27.647783251231527 26.502463054187192 24.950738916256157 16 21.09605911330049 38.55911330049261 22.86945812807882 17.47536945812808 17 10.456952826702796 48.41729277004557 28.747382682596378 12.378371720655252 18 20.61576354679803 29.901477832512313 38.12807881773399 11.354679802955665 19 9.556650246305418 36.51477832512315 33.07881773399014 20.84975369458128 20 8.854679802955664 30.751231527093598 34.002463054187196 26.391625615763548 21 4.125615763546798 39.43349753694581 33.830049261083744 22.610837438423644 22 4.458128078817734 52.52463054187192 34.80295566502463 8.214285714285714 23 5.467980295566503 36.42857142857142 36.03448275862069 22.06896551724138 24 19.100985221674875 39.96305418719212 36.55172413793103 4.384236453201971 25 7.967980295566503 50.97290640394089 34.83990147783251 6.219211822660099 26 17.47752186229831 39.807858110604755 36.827195467422094 5.8874245596748365 27 5.172413793103448 52.83251231527094 36.65024630541872 5.344827586206897 28 3.6083743842364533 38.7192118226601 38.32512315270936 19.34729064039409 29 4.298029556650246 40.41871921182266 50.51724137931034 4.766009852216749 30 4.655172413793104 51.66256157635468 37.64778325123152 6.0344827586206895 31 5.197044334975369 39.76600985221675 37.06896551724138 17.967980295566502 32 4.766009852216749 39.618226600985224 50.504926108374384 5.110837438423645 33 5.246305418719212 40.837438423645324 50.01231527093596 3.903940886699507 34 17.45073891625616 41.24384236453202 35.467980295566505 5.83743842364532 35 3.9285714285714284 42.54926108374384 35.88669950738917 17.635467980295566 36 3.7561576354679804 56.477832512315274 35.56650246305419 4.199507389162561 37 3.2389162561576352 55.1231527093596 37.29064039408867 4.347290640394089 38 3.793103448275862 41.293103448275865 48.76847290640394 6.1453201970443345 39 3.8793103448275863 41.09605911330049 36.83497536945813 18.189655172413794 40 4.064039408866995 40.78817733990148 48.96551724137931 6.182266009852216 41 5.1600985221674875 41.1576354679803 35.172413793103445 18.50985221674877 42 17.943349753694584 41.145320197044335 36.1576354679803 4.753694581280788 43 4.014778325123153 42.68472906403941 48.84236453201971 4.458128078817734 44 3.9408866995073892 54.92610837438424 36.18226600985221 4.9507389162561575 45 5.20935960591133 41.42857142857143 47.86945812807882 5.4926108374384235 46 18.3128078817734 40.43103448275862 34.26108374384236 6.995073891625616 47 4.667487684729064 41.19458128078818 33.546798029556655 20.591133004926107 48 5.788177339901478 40.43103448275862 33.546798029556655 20.233990147783253 49 18.645320197044335 40.923645320197046 35.03694581280788 5.394088669950739 50 4.261083743842365 40.29556650246305 47.881773399014776 7.5615763546798025 51 4.199507389162561 40.94827586206897 34.83990147783251 20.01231527093596 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 607.0 1 329.0 2 51.0 3 108.0 4 165.0 5 152.5 6 140.0 7 207.0 8 274.0 9 226.0 10 178.0 11 276.0 12 374.0 13 585.0 14 796.0 15 834.0 16 872.0 17 647.0 18 422.0 19 340.0 20 258.0 21 403.5 22 549.0 23 361.5 24 174.0 25 179.0 26 273.5 27 363.0 28 230.0 29 97.0 30 87.5 31 78.0 32 85.0 33 92.0 34 101.5 35 111.0 36 118.5 37 126.0 38 128.5 39 131.0 40 127.0 41 123.0 42 106.0 43 89.0 44 99.5 45 110.0 46 97.0 47 84.0 48 171.5 49 259.0 50 728.0 51 1197.0 52 633.5 53 70.0 54 58.5 55 47.0 56 40.0 57 33.0 58 28.0 59 23.0 60 19.5 61 16.0 62 13.5 63 11.0 64 8.5 65 6.0 66 5.5 67 5.0 68 4.0 69 3.0 70 2.0 71 1.0 72 1.0 73 1.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02463054187192118 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.01231527093596059 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.01231527093596059 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 8120.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.77832512315271 #Duplication Level Percentage of deduplicated Percentage of total 1 90.2812241521919 26.88423645320197 2 3.2671629445823 1.9458128078817736 3 1.3234077750206783 1.1822660098522169 4 0.6617038875103392 0.7881773399014778 5 0.41356492969396197 0.6157635467980296 6 0.3308519437551696 0.5911330049261084 7 0.24813895781637718 0.5172413793103449 8 0.3308519437551696 0.7881773399014778 9 0.20678246484698098 0.5541871921182266 >10 2.109181141439206 14.544334975369457 >50 0.3308519437551696 7.06896551724138 >100 0.41356492969396197 24.34729064039409 >500 0.0413564929693962 7.056650246305418 >1k 0.0413564929693962 13.115763546798028 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC 1065 13.115763546798028 TruSeq Adapter, Index 12 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 573 7.056650246305418 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 486 5.985221674876847 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 265 3.263546798029557 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 258 3.1773399014778327 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 201 2.4753694581280787 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 156 1.921182266009852 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 145 1.7857142857142856 No Hit TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT 123 1.5147783251231526 TruSeq Adapter, Index 12 (95% over 22bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 123 1.5147783251231526 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 112 1.3793103448275863 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 108 1.3300492610837438 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 95 1.1699507389162562 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 94 1.1576354679802956 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 78 0.960591133004926 No Hit GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 75 0.9236453201970444 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 67 0.8251231527093595 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 58 0.7142857142857143 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56 0.6896551724137931 No Hit GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 51 0.6280788177339901 No Hit GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 50 0.6157635467980296 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 48 0.5911330049261084 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 47 0.5788177339901478 No Hit ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 46 0.5665024630541872 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 45 0.5541871921182266 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 42 0.5172413793103449 No Hit ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA 39 0.480295566502463 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 37 0.45566502463054187 No Hit TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 34 0.41871921182266003 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 33 0.4064039408866995 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 33 0.4064039408866995 No Hit GCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 32 0.3940886699507389 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32 0.3940886699507389 No Hit CCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 31 0.38177339901477836 No Hit CTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 29 0.35714285714285715 No Hit GTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 29 0.35714285714285715 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA 28 0.3448275862068966 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28 0.3448275862068966 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAA 28 0.3448275862068966 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25 0.3078817733990148 No Hit CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 24 0.2955665024630542 No Hit TACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22 0.270935960591133 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 22 0.270935960591133 No Hit TACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21 0.25862068965517243 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20 0.24630541871921183 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC 20 0.24630541871921183 TruSeq Adapter, Index 12 (95% over 24bp) ACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.23399014778325125 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.22167487684729065 No Hit ATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 17 0.20935960591133002 No Hit TATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTT 17 0.20935960591133002 RNA PCR Primer, Index 12 (95% over 23bp) AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAA 17 0.20935960591133002 No Hit GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.19704433497536944 No Hit GATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.19704433497536944 No Hit GCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.18472906403940886 No Hit TGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.1724137931034483 No Hit ATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 14 0.1724137931034483 No Hit TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT 14 0.1724137931034483 No Hit GTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.16009852216748768 No Hit TACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.16009852216748768 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 13 0.16009852216748768 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.1477832512315271 No Hit CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.1477832512315271 No Hit AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12 0.1477832512315271 No Hit GATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.1477832512315271 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.1354679802955665 No Hit CCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 11 0.1354679802955665 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10 0.12315270935960591 No Hit TTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10 0.12315270935960591 No Hit GTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAA 10 0.12315270935960591 No Hit CTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10 0.12315270935960591 No Hit CTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10 0.12315270935960591 No Hit TTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.11083743842364532 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAA 9 0.11083743842364532 No Hit GCGTTGATACCACTGCTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.11083743842364532 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 9 0.11083743842364532 No Hit ACTCTGCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAA 9 0.11083743842364532 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.012315270935960592 16 0.0 0.0 0.024630541871921183 17 0.0 0.0 0.03694581280788178 18 0.0 0.0 0.03694581280788178 19 0.0 0.0 0.06157635467980296 20 0.0 0.0 0.06157635467980296 21 0.0 0.0 0.08620689655172414 22 0.0 0.0 0.08620689655172414 23 0.0 0.0 0.11083743842364532 24 0.0 0.0 0.1354679802955665 25 0.0 0.0 0.16009852216748768 26 0.0 0.0 0.22167487684729065 27 0.0 0.0 0.270935960591133 28 0.0 0.0 0.33251231527093594 29 0.0 0.0 0.4187192118226601 30 0.0 0.0 0.49261083743842365 31 0.0 0.0 0.6773399014778325 32 0.0 0.0 0.8620689655172413 33 0.0 0.0 1.0344827586206897 34 0.0 0.0 1.416256157635468 35 0.0 0.0 1.5270935960591132 36 0.0 0.0 1.9334975369458127 37 0.0 0.0 2.1305418719211824 38 0.0 0.0 2.29064039408867 39 0.0 0.0 2.438423645320197 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAAAAA 25 3.5575737E-5 45.0 19 TGCTTCC 20 6.6261896E-4 45.0 14 TTCCAAA 25 3.5575737E-5 45.0 17 TCCAAAA 25 3.5575737E-5 45.0 18 GCTTCCA 20 6.6261896E-4 45.0 15 CTTCCAA 25 3.5575737E-5 45.0 16 TGCCGTC 125 0.0 39.6 45 TATGCCG 125 0.0 39.6 43 ATGCCGT 125 0.0 39.6 44 TTGCAAT 120 0.0 39.374996 32 AATCTCG 120 0.0 39.374996 36 TGCAATC 120 0.0 39.374996 33 CAATCTC 120 0.0 39.374996 35 GCAATCT 120 0.0 39.374996 34 AGCCCAC 130 0.0 38.07692 17 GTATGCC 130 0.0 38.07692 42 ATCTCGT 130 0.0 38.07692 37 ATCTCCG 130 0.0 38.07692 10 GCCCACG 130 0.0 38.07692 18 CCACGAG 130 0.0 38.07692 20 >>END_MODULE