FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_11.341000000068cf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_11.341000000068cf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8120
Sequences flagged as poor quality0
Sequence length51
%GC25

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC106513.115763546798028TruSeq Adapter, Index 12 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5737.056650246305418No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4865.985221674876847No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2653.263546798029557No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2583.1773399014778327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2012.4753694581280787No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1561.921182266009852No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1451.7857142857142856No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT1231.5147783251231526TruSeq Adapter, Index 12 (95% over 22bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1231.5147783251231526No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1121.3793103448275863No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1081.3300492610837438No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT951.1699507389162562No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT941.1576354679802956No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.960591133004926No Hit
GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA750.9236453201970444No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.8251231527093595No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.7142857142857143No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT560.6896551724137931No Hit
GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.6280788177339901No Hit
GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.6157635467980296No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.5911330049261084No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA470.5788177339901478No Hit
ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.5665024630541872No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.5541871921182266No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA420.5172413793103449No Hit
ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA390.480295566502463No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370.45566502463054187No Hit
TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA340.41871921182266003No Hit
GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.4064039408866995No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA330.4064039408866995No Hit
GCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA320.3940886699507389No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.3940886699507389No Hit
CCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.38177339901477836No Hit
CTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.35714285714285715No Hit
GTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA290.35714285714285715No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA280.3448275862068966No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.3448275862068966No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAA280.3448275862068966No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250.3078817733990148No Hit
CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.2955665024630542No Hit
TACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.270935960591133No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.270935960591133No Hit
TACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.25862068965517243No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.24630541871921183No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC200.24630541871921183TruSeq Adapter, Index 12 (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAA190.23399014778325125No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.22167487684729065No Hit
ATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA170.20935960591133002No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTT170.20935960591133002RNA PCR Primer, Index 12 (95% over 23bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAA170.20935960591133002No Hit
GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.19704433497536944No Hit
GATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.19704433497536944No Hit
GCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.18472906403940886No Hit
TGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1724137931034483No Hit
ATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.1724137931034483No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT140.1724137931034483No Hit
GTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.16009852216748768No Hit
TACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.16009852216748768No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC130.16009852216748768No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1477832512315271No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1477832512315271No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.1477832512315271No Hit
GATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.1477832512315271No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.1354679802955665No Hit
CCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.1354679802955665No Hit
TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.12315270935960591No Hit
TTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.12315270935960591No Hit
GTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAA100.12315270935960591No Hit
CTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.12315270935960591No Hit
CTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.12315270935960591No Hit
TTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.11083743842364532No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAA90.11083743842364532No Hit
GCGTTGATACCACTGCTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.11083743842364532No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT90.11083743842364532No Hit
ACTCTGCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAA90.11083743842364532No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAAAAA253.5575737E-545.019
TGCTTCC206.6261896E-445.014
TTCCAAA253.5575737E-545.017
TCCAAAA253.5575737E-545.018
GCTTCCA206.6261896E-445.015
CTTCCAA253.5575737E-545.016
TGCCGTC1250.039.645
TATGCCG1250.039.643
ATGCCGT1250.039.644
TTGCAAT1200.039.37499632
AATCTCG1200.039.37499636
TGCAATC1200.039.37499633
CAATCTC1200.039.37499635
GCAATCT1200.039.37499634
AGCCCAC1300.038.0769217
GTATGCC1300.038.0769242
ATCTCGT1300.038.0769237
ATCTCCG1300.038.0769210
GCCCACG1300.038.0769218
CCACGAG1300.038.0769220
Produced by FastQC (version 0.11.2)