##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_10.341000000068b2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 27785 Sequences flagged as poor quality 0 Sequence length 51 %GC 20 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.44578009717473 38.0 38.0 38.0 32.0 38.0 2 36.293935576750044 38.0 38.0 38.0 32.0 38.0 3 36.33233759222602 38.0 38.0 38.0 32.0 38.0 4 36.32517545438186 38.0 38.0 38.0 32.0 38.0 5 36.36303761022134 38.0 38.0 38.0 32.0 38.0 6 38.319524923519886 40.0 38.0 40.0 38.0 40.0 7 38.24621198488393 40.0 38.0 40.0 38.0 40.0 8 38.23480295123268 40.0 38.0 40.0 38.0 40.0 9 38.163469497930535 40.0 38.0 40.0 38.0 40.0 10 38.10332913442505 40.0 38.0 40.0 38.0 40.0 11 38.04200107971927 40.0 38.0 40.0 32.0 40.0 12 37.970199748065504 40.0 38.0 40.0 32.0 40.0 13 37.8490192549937 40.0 38.0 40.0 32.0 40.0 14 37.810365305020696 40.0 38.0 40.0 32.0 40.0 15 37.770739607702 40.0 38.0 40.0 32.0 40.0 16 37.795069281986684 40.0 38.0 40.0 32.0 40.0 17 37.83077199928019 40.0 38.0 40.0 32.0 40.0 18 37.857045168256256 40.0 38.0 40.0 32.0 40.0 19 38.0132085657729 40.0 38.0 40.0 32.0 40.0 20 37.99103833003419 40.0 38.0 40.0 32.0 40.0 21 38.05409393557675 40.0 38.0 40.0 32.0 40.0 22 38.06237178333633 40.0 38.0 40.0 32.0 40.0 23 38.05628936476516 40.0 38.0 40.0 32.0 40.0 24 38.004174914522224 40.0 38.0 40.0 32.0 40.0 25 37.96883210365305 40.0 38.0 40.0 32.0 40.0 26 37.907108151880514 40.0 38.0 40.0 32.0 40.0 27 37.853086197588624 40.0 38.0 40.0 32.0 40.0 28 37.80691020334713 40.0 38.0 40.0 32.0 40.0 29 37.74504228900486 40.0 38.0 40.0 32.0 40.0 30 37.740291524203705 40.0 38.0 40.0 32.0 40.0 31 37.71376642073061 40.0 38.0 40.0 32.0 40.0 32 37.6522944034551 40.0 38.0 40.0 32.0 40.0 33 37.61072521144502 40.0 38.0 40.0 32.0 40.0 34 37.541731149901025 40.0 38.0 40.0 32.0 40.0 35 37.499766060824186 40.0 38.0 40.0 32.0 40.0 36 37.40233939175814 40.0 38.0 40.0 32.0 40.0 37 37.36019434946914 40.0 38.0 40.0 32.0 40.0 38 37.27658808709735 40.0 38.0 40.0 32.0 40.0 39 37.140399496131 40.0 38.0 40.0 32.0 40.0 40 37.08371423429908 40.0 38.0 40.0 32.0 40.0 41 37.05704516825625 40.0 38.0 40.0 32.0 40.0 42 36.92884649991002 40.0 38.0 40.0 32.0 40.0 43 36.849811049127226 40.0 38.0 40.0 32.0 40.0 44 36.70559654489833 40.0 38.0 40.0 27.0 40.0 45 36.575814288285045 40.0 38.0 40.0 27.0 40.0 46 36.42058664747166 40.0 38.0 40.0 27.0 40.0 47 36.32668706136405 40.0 38.0 40.0 27.0 40.0 48 36.194529422350186 40.0 38.0 40.0 27.0 40.0 49 36.0095015296023 40.0 38.0 40.0 27.0 40.0 50 35.949613100593844 40.0 38.0 40.0 27.0 40.0 51 35.978261651970485 40.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 -0.30301115530012623 2201 2 -0.8036425925150752 2201 3 0.4326670361266167 2201 4 0.3684085210335226 2201 5 -0.19514301508812792 2201 6 -0.4120047073931019 2201 7 0.5682754568322679 2201 8 0.22178791107709372 2201 9 -0.13918853460270242 2201 10 0.7069084438405255 2201 11 0.8146720993850138 2201 12 0.7483828504924332 2201 13 0.06250118129874238 2201 14 -0.375012847575249 2201 15 -0.23088035842997812 2201 16 -0.36224083837294074 2201 17 -0.4639779504034749 2201 18 0.05073539099052482 2201 19 -1.1203333101614206 2201 20 1.0800556535733534 2201 21 0.7168192415163617 2201 22 0.2776790449207809 2201 23 0.12305053286172551 2201 24 1.0317644539035555 2201 25 -0.11216105366465001 2201 26 -1.395666534955268 2201 27 -0.5822690328300908 2201 28 -0.2615890997267627 2201 29 0.2339643160999998 2201 30 0.3342284116331058 2201 31 0.18755171190176867 2201 32 0.470939644458241 2201 33 -1.3828794752943452 2201 34 -0.5503374212507026 2201 35 0.2502613761471366 2201 36 -0.8118851805542775 2201 37 0.1475855151486698 2201 38 0.262452464890103 2201 39 0.965795340998092 2201 40 0.4926200692857279 2201 41 0.3938376695284518 2201 42 0.618921757394439 2201 43 0.55627432060502 2201 44 0.985414476781358 2201 45 -0.03549714681911098 2201 46 -0.35728931428000976 2201 47 0.1306690874910288 2201 48 0.25755250267994256 2201 49 0.3393409760430899 2201 50 0.052711490989381105 2201 51 -0.747636414343404 1101 1 0.8969888446998766 1101 2 0.4820716931992095 1101 3 0.9755241789837612 1101 4 0.8826942353192422 1101 5 0.06199984205472475 1101 6 0.01656672117832869 1101 7 0.08256117111798744 1101 8 0.5932164825056603 1101 9 -0.2820456774598483 1101 10 -0.17880584187375348 1101 11 -0.6996136149006986 1101 12 0.7483828504924332 1101 13 1.462501181298748 1101 14 0.25355858099617734 1101 15 0.2262624987128845 1101 16 0.46633059019848844 1101 17 -0.6068350932606137 1101 18 0.3078782481333846 1101 19 -0.8917618815899928 1101 20 -0.2628014892837953 1101 21 -0.2546093299122063 1101 22 0.6776790449207795 1101 23 1.2944791042902963 1101 24 1.2031930253321335 1101 25 1.2021246606210667 1101 26 0.2043334650447335 1101 27 0.2463023957413384 1101 28 0.6241251859875234 1101 29 0.17682145895714285 1101 30 -0.09434301693832481 1101 31 0.9018374261874769 1101 32 0.1852253587439492 1101 33 0.7599776675628007 1101 34 0.3353768644635835 1101 35 1.2216899475757117 1101 36 -1.0118851805542803 1101 37 0.4047283722915225 1101 38 0.8053096077472475 1101 39 -0.091347516144765 1101 40 0.7783343550000126 1101 41 0.5652662409570226 1101 42 0.9617789002515806 1101 43 0.55627432060502 1101 44 0.8425573339242121 1101 45 0.5359314246094655 1101 46 -0.8715750285657293 1101 47 -0.18361662679468083 1101 48 0.22898107410851054 1101 49 -0.2035161668140475 1101 50 -0.3472885090106246 1101 51 -1.4047792714862624 2202 1 -0.20108065336962255 2202 2 -0.15731054618303375 2202 3 -0.09551829205871343 2202 4 -0.0709737183487178 2202 5 0.23265621271109893 2202 6 0.4860647906763944 2202 7 0.4377735263303393 2202 8 0.68201957130875 2202 9 0.07085007543590649 2202 10 0.9818119187440004 2202 11 0.33127441598733043 2202 12 -0.0215013193917315 2202 13 0.46945098824855336 2202 14 0.7068404342780283 2202 15 1.2787721512225332 2202 16 0.8547475786154735 2202 17 1.0008869144613897 2202 18 1.1603879006430375 2202 19 -0.06010164992976286 2202 20 0.7510981246158224 2202 21 0.9979003225974452 2202 22 -0.34008157283983564 2202 23 0.49061810042928755 2202 24 0.7900656122047138 2202 25 0.8430513015477032 2202 26 0.2552987160099818 2202 27 0.8061479555868942 2202 28 0.9762487381110745 2202 29 0.823925706061388 2202 30 1.056236133640823 2202 31 0.8076289319789822 2202 32 1.2979666714852627 2202 33 0.6225259301110597 2202 34 -0.8808393517526341 2202 35 -0.06325213736637636 2202 36 0.7263387576696587 2202 37 0.22634999391314636 2202 38 1.067857870295505 2202 39 0.04378761899037187 2202 40 0.21694439361004925 2202 41 1.025497901188679 2202 42 1.0505819890546704 2202 43 1.0134171777478755 2202 44 -0.3265546351877546 2202 45 0.6934603821384187 2202 46 -0.11867927566997594 2202 47 0.1538351106570559 2202 48 1.2011818463092823 2202 49 0.9030475397496573 2202 50 0.29132152959941493 2202 51 1.8569967902897986 1102 1 -0.08396353625250441 1102 2 0.5146113757388946 1102 3 -0.1998726464130698 1102 4 -1.3434962408712394 1102 5 -0.2808573008024169 1102 6 -1.0199412153296095 1102 7 -0.3182324796756646 1102 8 -0.2917041524149724 1102 9 -1.1145853599995306 1102 10 0.23706717399925736 1102 11 0.20514828986120648 1102 12 0.42219237430195733 1102 13 -0.8255940567964899 1102 14 -0.6662826888450937 1102 15 0.4199132923636739 1102 16 -0.29239956853167115 1102 17 0.10824427181874796 1102 18 0.07930681956195684 1102 19 -0.422714262542371 1102 20 -0.6723252988075998 1102 21 0.13904146373858595 1102 22 0.4046631719049074 1102 23 0.2286060884172798 1102 24 1.0603358824749876 1102 25 -0.24628803779163633 1102 26 0.39719060790187655 1102 27 0.4367785862175282 1102 28 -0.6949224330600998 1102 29 -0.29619441405873204 1102 30 0.05248237988707416 1102 31 -0.34101971666966335 1102 32 -0.20128257776398328 1102 33 0.5782316358167705 1102 34 0.36315464224136207 1102 35 -0.4386275127417463 1102 36 1.0341465654774638 1102 37 -0.8674938499306961 1102 38 1.0791191315567659 1102 39 0.21420803941079214 1102 40 -0.5240465973809378 1102 41 -0.1982258225350435 1102 42 -0.9786972902246092 1102 43 -0.18102726669656732 1102 44 1.0750970164639 1102 45 0.31132825000629083 1102 46 -0.7763369333276344 1102 47 0.1425738493957951 1102 48 -1.1186379735105376 1102 49 1.4623568490589633 1102 50 0.18921942749731357 1102 51 0.826966760259765 2203 1 1.007703130414157 2203 2 0.4535002646277775 2203 3 0.4862384646980473 2203 4 0.6898370924620991 2203 5 1.2691426991975803 2203 6 0.952281006892612 2203 7 1.3039897425465554 2203 8 1.2860736253628033 2203 9 1.1465257511115823 2203 10 0.7676227295548159 2203 11 0.6968149565278736 2203 12 0.08052570763529587 2203 13 0.5160726098701716 2203 14 0.8642728667104649 2203 15 1.347691070141451 2203 16 1.2270448759127746 2203 17 1.4360220495965237 2203 18 1.4793068195619554 2203 19 1.5439524041242905 2203 20 1.644341367859063 2203 21 1.316819241516363 2203 22 1.3491076163493503 2203 23 1.3730505328617255 2203 24 1.1103358824749847 2203 25 1.2092675177639194 2203 26 0.7721906079018765 2203 27 1.0284452528841896 2203 28 1.3384109002732387 2203 29 1.1732500303857094 2203 30 1.5163712687759627 2203 31 1.5589802833303352 2203 32 1.4209396444582367 2203 33 0.9921205247056548 2203 34 1.6353768644635807 2203 35 1.2752613761471423 2203 36 1.0452576765885766 2203 37 2.054728372291528 2203 38 1.762452464890103 2203 39 1.8336524838552322 2203 40 2.0176200692857265 2203 41 1.7545519552427322 2203 42 2.204636043108728 2203 43 2.2134171777478784 2203 44 1.9639859053527857 2203 45 1.9752171388951751 2203 46 2.7069964000057 2203 47 1.9842405160624565 2203 48 2.3146953598227995 2203 49 2.1429124046145205 2203 50 2.841997205275092 2203 51 2.2380778713708764 1103 1 0.29322944620363245 1103 2 -0.42544710379327455 1103 3 0.2138700436454144 1103 4 0.055626566146308676 1103 5 0.3507216465660008 1103 6 -0.21877162468633315 1103 7 -0.2670628890323883 1103 8 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1.7929332737738406 1103 45 1.0028487178425465 1103 46 1.8464700842162287 1103 47 1.1118720950098293 1103 48 1.5041690440333255 1103 49 1.8731755625092603 1103 50 0.9669972052750921 1103 51 1.4643936608445642 2204 1 -1.0082059604949336 2204 2 -0.5964997353722197 2204 3 -0.8160342625746821 2204 4 -0.6692538166288102 2204 5 -1.6899482098933234 2204 6 -1.8431735385619348 2204 7 -0.4141920756352633 2204 8 0.6906190799082594 2204 9 0.3147075692934038 2204 10 -0.18464999771791213 2204 11 0.030905865618777284 2204 12 0.21007116218074628 2204 13 0.3410726098701744 2204 14 -0.08799986056226317 2204 15 0.4476910701414525 2204 16 -0.8025005786326815 2204 17 -0.04579613222165335 2204 18 -0.4661477258925899 2204 19 0.31213422230611343 2204 20 1.085250458768158 2204 21 0.07591015060727102 2204 22 0.3491076163493503 2204 23 -1.5314949216837306 2204 24 -0.5533004811613793 2204 25 -0.3725506640542591 2204 26 0.30628151699278305 2204 27 -0.46473656529762764 2204 28 0.002047263909602748 2204 29 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1213 51 1.095972608212982 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 5.0 17 7.0 18 4.0 19 2.0 20 9.0 21 21.0 22 32.0 23 57.0 24 72.0 25 127.0 26 154.0 27 236.0 28 311.0 29 368.0 30 447.0 31 609.0 32 739.0 33 899.0 34 1055.0 35 1289.0 36 1614.0 37 2138.0 38 3987.0 39 13603.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.57367132363532 27.754572951173845 13.859282730808008 13.812472994382832 2 8.390022675736962 31.59126084296152 33.038188820501745 26.98052766079977 3 22.670505668526182 37.61022134245096 31.94529422350189 7.7739787655209645 4 9.929818247255714 25.924059744466437 35.95105272629117 28.195069281986683 5 11.203886989382761 36.86161597984524 32.69749865035091 19.236998380421092 6 28.000719812848658 41.079719272989024 10.462479755263631 20.457081158898685 7 8.299442145042288 47.91074320676624 20.52906244376462 23.26075220442685 8 19.654489832643513 35.87907144142523 24.0849379161418 20.381500809789454 9 17.117149541119307 31.02033471297463 22.57692999820047 29.285585747705596 10 12.575130466078818 37.71459420550657 24.635594745366205 25.07468058304841 11 18.29176114890401 34.01720476550408 20.256991685563115 27.434042400028797 12 17.329494331473818 40.78819506928198 28.349829044448445 13.532481554795753 13 24.416051826525102 31.088716933597265 27.039769659888428 17.455461579989205 14 17.642612920640634 33.02861256073421 32.74428648551377 16.58448803311139 15 21.248875292423968 33.47129746265971 30.815188051106713 14.464639193809608 16 9.451142702897247 44.11732949433148 27.43566672665107 18.99586107612021 17 9.994601403635055 41.52960230340111 34.52222422170236 13.953572071261473 18 10.142163037610223 33.62965628936477 43.685441785135865 12.542738887889149 19 9.739067842360987 41.50080978945474 39.60050386899406 9.15961849919021 20 8.562173834802952 34.84614000359906 41.78513586467518 14.806550296922799 21 4.066942594925319 42.60572251214684 40.924959510527266 12.402375382400574 22 5.906064423249955 44.09573510887169 41.461220082778475 8.536980385099874 23 4.300881770739608 39.07863955371603 43.57387079359366 13.046607881950692 24 7.3384919920820595 43.76462119848839 44.196508907684 4.700377901745546 25 7.525643332733489 42.371783336332555 42.47975526363146 7.622818067302502 26 6.402965735675209 41.78663979268644 45.55139648718687 6.258997984451482 27 7.529242396976786 43.494691380241136 44.73996760842181 4.236098614360266 28 3.9661687961130108 41.23447903545078 45.26903005218643 9.530322116249774 29 4.678783516285765 42.648911283066404 48.00791794133525 4.664387259312579 30 4.592571264036856 43.877771379211055 45.38583357327959 6.143823783472502 31 5.405794493431708 42.3105992441965 44.54561813928379 7.737988123087996 32 4.891128306640273 43.001619578909484 46.26956991182293 5.837682202627317 33 4.257692999820047 45.03509087637214 46.00323915781897 4.7039769659888435 34 6.219183012416772 45.9636494511427 43.57027172935037 4.246895807090157 35 4.106532301601583 45.77289904624798 43.296742846859814 6.823825805290625 36 4.149721072521144 48.59816447723592 43.21756343350729 4.034551016735648 37 3.6998380421090515 48.10869174014756 43.39751664567213 4.793953572071262 38 3.8941875112470754 46.20118769120029 45.54615799892028 4.358466798632356 39 4.340471477415872 45.79449343170776 43.04480834982905 6.820226741047327 40 4.095735108871693 46.01403635054886 45.05308619758863 4.8371423429908225 41 5.024293683642253 45.67572431167896 42.677703797012775 6.622278207666008 42 6.44232499550117 46.06082418571171 43.01601583588267 4.480834982904445 43 4.239697678603563 46.71225481374843 44.54201907504049 4.506028432607522 44 4.365664927118949 48.43980565053086 42.335792693899585 4.858736728450603 45 4.524023753824006 46.05722512146842 44.35486773438906 5.063883390318518 46 7.165736908403815 45.81968688141083 41.677163937376285 5.337412272809069 47 4.560014396256974 45.9636494511427 41.6555695519165 7.820766600683822 48 4.927118949073241 46.22278207666007 40.95015296023034 7.899946014036351 49 7.212524743566673 46.03203167176534 41.34245096274968 5.412992621918302 50 4.8371423429908225 46.00323915781897 43.50188950872773 5.65772899046248 51 4.880331113910383 45.967248515386 40.8817707396077 8.270649631095916 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2049.0 1 1131.0 2 213.0 3 434.5 4 656.0 5 701.5 6 747.0 7 1082.5 8 1418.0 9 1107.0 10 796.0 11 1247.5 12 1699.0 13 2315.0 14 2931.0 15 3192.0 16 3453.0 17 2544.0 18 1635.0 19 1337.0 20 1039.0 21 1396.0 22 1753.0 23 1359.0 24 965.0 25 817.5 26 1103.0 27 1536.0 28 1214.0 29 892.0 30 595.5 31 299.0 32 289.0 33 279.0 34 311.5 35 344.0 36 340.5 37 337.0 38 309.0 39 281.0 40 312.5 41 344.0 42 403.0 43 462.0 44 416.0 45 370.0 46 359.0 47 348.0 48 388.5 49 429.0 50 726.0 51 1023.0 52 629.5 53 236.0 54 203.5 55 171.0 56 157.0 57 143.0 58 121.0 59 99.0 60 84.5 61 70.0 62 54.0 63 38.0 64 30.0 65 22.0 66 19.5 67 17.0 68 13.5 69 10.0 70 7.5 71 5.0 72 4.5 73 4.0 74 2.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.5 82 1.0 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04678783516285766 2 0.007198128486593485 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.007198128486593485 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0035990642432967425 27 0.0 28 0.0 29 0.0 30 0.0035990642432967425 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 27785.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 27.068562173834803 #Duplication Level Percentage of deduplicated Percentage of total 1 91.54367770243319 24.779557315098074 2 3.7096130833665737 2.0082778477595826 3 0.9706156096263794 0.7881950692819866 4 0.7046935247972343 0.7630016195789094 5 0.5717324823826619 0.7737988123087997 6 0.23932987634623054 0.38869893827604823 7 0.17284935513894428 0.3275148461400036 8 0.09307272969020078 0.2015475976246176 9 0.07977662544874352 0.19434946913802412 >10 1.1434649647653239 6.409933417311499 >50 0.3191065017949741 5.9024653590066585 >100 0.3191065017949741 19.69048047507648 >500 0.09307272969020078 19.157818967068565 >1k 0.03988831272437176 18.614360266330753 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1991 7.165736908403815 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1966 7.075760302321396 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1215 4.372863055605543 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 952 3.426309159618499 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 942 3.390318517185532 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 900 3.2391578189670684 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 818 2.9440345510167356 No Hit CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC 676 2.432967428468598 TruSeq Adapter, Index 12 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 526 1.8931077919740869 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 509 1.8319236998380424 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 500 1.7995321216483715 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 446 1.6051826525103472 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 428 1.540399496131006 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 349 1.2560734209105633 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 340 1.2236818427208926 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 340 1.2236818427208926 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 301 1.0833183372323196 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 251 0.9033651250674825 No Hit GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 247 0.8889688680942954 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 225 0.8097894547417671 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 224 0.8061903904984704 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 176 0.6334353068202268 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 171 0.615439985603743 No Hit CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 157 0.5650530861975886 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 155 0.5578549577109951 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 153 0.5506568292244016 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAA 150 0.5398596364945114 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 142 0.5110671225481375 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 141 0.5074680583048408 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 135 0.4858736728450603 No Hit ACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAA 113 0.40669425949253196 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAA 112 0.4030951952492352 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 111 0.39949613100593845 No Hit TTTTTGCTCTGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 104 0.37430268130286126 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 100 0.3599064243296743 No Hit TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT 91 0.3275148461400036 TruSeq Adapter, Index 12 (95% over 22bp) GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 83 0.29872233219362965 No Hit GCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 83 0.29872233219362965 No Hit TACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 82 0.29512326795033295 No Hit CTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 82 0.29512326795033295 No Hit GCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 79 0.2843260752204427 No Hit GTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 74 0.26633075400395895 No Hit GAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 73 0.26273168976066225 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 71 0.25553356127406873 No Hit TGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 69 0.24833543278747527 No Hit GTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAA 68 0.24473636854417852 No Hit ATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 67 0.24113730430088176 No Hit GCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 66 0.23753824005758503 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 60 0.21594385459780457 No Hit GCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 59 0.21234479035450782 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 57 0.20514666186791436 No Hit AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 56 0.2015475976246176 No Hit GCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 55 0.19794853338132085 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 54 0.19434946913802412 No Hit GGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 54 0.19434946913802412 No Hit TTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 53 0.19075040489472736 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 53 0.19075040489472736 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 51 0.18355227640813387 No Hit CTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 49 0.17635414792154042 No Hit GCGTTGATACCACTGCTTCAAGCAGTGGTATCAACGCAGAGTACTTTTTTT 49 0.17635414792154042 No Hit CTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 47 0.16915601943494693 No Hit CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44 0.1583588267050567 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42 0.1511606982184632 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAA 42 0.1511606982184632 No Hit GCGTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42 0.1511606982184632 No Hit GCGTTGATACCACTGCTTGAAGCAGTGGTATCAACGCAGAGTACTTTTTTT 40 0.14396256973186972 No Hit GCTTAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 39 0.14036350548857296 No Hit TTTTGGACCTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 37 0.13316537700197947 No Hit CTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 35 0.12596724851538602 No Hit GCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 31 0.11157099154219903 No Hit GTACTCTGCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAA 31 0.11157099154219903 No Hit CCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 30 0.10797192729890229 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC 30 0.10797192729890229 TruSeq Adapter, Index 12 (95% over 24bp) AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAA 29 0.10437286305560553 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.003599064243296743 12 0.0 0.0 0.017995321216483715 13 0.0 0.0 0.028792513946373943 14 0.0 0.0 0.032391578189670686 15 0.0 0.0 0.03599064243296743 16 0.0 0.0 0.0503868994061544 17 0.0 0.0 0.057585027892747886 18 0.0 0.0 0.07198128486593486 19 0.0 0.0 0.08637754183912183 20 0.0 0.0 0.09717473456901206 21 0.0 0.0 0.10437286305560554 22 0.0 0.0 0.11517005578549577 23 0.0 0.0 0.13676444124527623 24 0.0 0.0 0.14396256973186972 25 0.0 0.0 0.14756163397516645 26 0.0 0.0 0.17275508367824366 27 0.0 0.0 0.19794853338132085 28 0.0 0.0 0.26633075400395895 29 0.0 0.0 0.30951952492351986 30 0.0 0.0 0.3275148461400036 31 0.0 0.0 0.45708115889868633 32 0.0 0.0 0.5290624437646212 33 0.0 0.0 0.6334353068202268 34 0.0 0.0 0.8241857117149541 35 0.0 0.0 0.9141623177973727 36 0.0 0.0 1.0689220802591326 37 0.0 0.0 1.1373043008817707 38 0.0 0.0 1.2344790354507829 39 0.0 0.0 1.385639733669246 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTCT 35 6.030863E-6 38.571426 45 GAGCAAA 40 1.5066142E-5 33.750004 1 AGCAAAA 40 1.5066142E-5 33.750004 2 CTTCAAG 35 2.7505297E-4 32.142857 2 TCAAGCA 35 2.7505297E-4 32.142857 4 GCTTCAA 35 2.7505297E-4 32.142857 1 TTGCAAT 95 0.0 30.789476 32 TGCAATC 95 0.0 30.789476 33 ACTTGCA 95 0.0 30.789476 30 TACTTGC 95 0.0 30.789476 29 CTACTTG 95 0.0 30.789476 28 GTATGCC 105 0.0 30.0 42 ATCTCGT 105 0.0 30.0 37 TGCCGTC 105 0.0 30.0 45 TATGCCG 105 0.0 30.0 43 TCTCGTA 105 0.0 30.0 38 ATGCCGT 105 0.0 30.0 44 CGTATGC 105 0.0 30.0 41 TCGTATG 105 0.0 30.0 40 CTCGTAT 105 0.0 30.0 39 >>END_MODULE