##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 48h_1.3410000000682f.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11546 Sequences flagged as poor quality 0 Sequence length 51 %GC 17 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.43079854495063 38.0 38.0 38.0 32.0 38.0 2 36.39442231075697 38.0 38.0 38.0 32.0 38.0 3 36.32063052139269 38.0 38.0 38.0 32.0 38.0 4 36.34176338125758 38.0 38.0 38.0 32.0 38.0 5 36.44898666204746 38.0 38.0 38.0 32.0 38.0 6 38.37666724406721 40.0 38.0 40.0 38.0 40.0 7 38.308331889831976 40.0 38.0 40.0 38.0 40.0 8 38.29031699289797 40.0 38.0 40.0 38.0 40.0 9 38.18785726658583 40.0 38.0 40.0 38.0 40.0 10 38.07275246838732 40.0 38.0 40.0 38.0 40.0 11 37.97791442923956 40.0 38.0 40.0 32.0 40.0 12 37.942664126104276 40.0 38.0 40.0 32.0 40.0 13 37.91408279923783 40.0 38.0 40.0 32.0 40.0 14 37.78425428719903 40.0 38.0 40.0 32.0 40.0 15 37.74813788324961 40.0 38.0 40.0 32.0 40.0 16 37.83188983197644 40.0 38.0 40.0 32.0 40.0 17 37.86177031006409 40.0 38.0 40.0 32.0 40.0 18 37.91356313874935 40.0 38.0 40.0 32.0 40.0 19 38.05196604884809 40.0 38.0 40.0 32.0 40.0 20 38.0482418153473 40.0 38.0 40.0 32.0 40.0 21 38.1349385068422 40.0 38.0 40.0 38.0 40.0 22 38.12307292568855 40.0 38.0 40.0 32.0 40.0 23 38.052658929499394 40.0 38.0 40.0 32.0 40.0 24 38.02199896067902 40.0 38.0 40.0 32.0 40.0 25 38.0167157457128 40.0 38.0 40.0 32.0 40.0 26 37.91988567469254 40.0 38.0 40.0 32.0 40.0 27 37.83310237311623 40.0 38.0 40.0 32.0 40.0 28 37.77550666897627 40.0 38.0 40.0 32.0 40.0 29 37.696258444482936 40.0 38.0 40.0 32.0 40.0 30 37.71747791442924 40.0 38.0 40.0 32.0 40.0 31 37.786332929152955 40.0 38.0 40.0 32.0 40.0 32 37.730556036722675 40.0 38.0 40.0 32.0 40.0 33 37.67391304347826 40.0 38.0 40.0 32.0 40.0 34 37.61960852243201 40.0 38.0 40.0 32.0 40.0 35 37.606530400138574 40.0 38.0 40.0 32.0 40.0 36 37.586350251169236 40.0 38.0 40.0 32.0 40.0 37 37.56894162480513 40.0 38.0 40.0 32.0 40.0 38 37.48787458860211 40.0 38.0 40.0 32.0 40.0 39 37.38065130781223 40.0 38.0 40.0 32.0 40.0 40 37.31214273341417 40.0 38.0 40.0 32.0 40.0 41 37.23939026502685 40.0 38.0 40.0 32.0 40.0 42 37.15321323402044 40.0 38.0 40.0 32.0 40.0 43 37.13251342456262 40.0 38.0 40.0 32.0 40.0 44 37.04122639875281 40.0 38.0 40.0 32.0 40.0 45 36.93478260869565 40.0 38.0 40.0 32.0 40.0 46 36.76112939546163 40.0 38.0 40.0 27.0 40.0 47 36.76597956002079 40.0 38.0 40.0 27.0 40.0 48 36.59613719036896 40.0 38.0 40.0 27.0 40.0 49 36.54365148103239 40.0 38.0 40.0 27.0 40.0 50 36.432963796985966 40.0 38.0 40.0 27.0 40.0 51 36.5783821236792 40.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2201 1 0.2187025214287317 2201 2 0.26124830084853556 2201 3 0.34805090174893394 2201 4 0.6893764322714873 2201 5 0.2300325184612575 2201 6 1.0272229334524852 2201 7 1.0959293553543574 2201 8 1.0550068765179645 2201 9 1.2380639221702054 2201 10 -2.2592443601166323 2201 11 -0.8483856781492349 2201 12 -1.4359131351973033 2201 13 -2.4326055312907116 2201 14 0.360079837125042 2201 15 0.7702487103789579 2201 16 0.2758601819211606 2201 17 1.0759638338718247 2201 18 0.630613643775348 2201 19 0.8970493860655395 2201 20 0.06803976737499795 2201 21 0.7495589484814644 2201 22 -0.41879343355290644 2201 23 0.6130515189018766 2201 24 0.6633961915365987 2201 25 -0.15360520283190482 2201 26 0.9738209786468701 2201 27 1.331934617867951 2201 28 1.5495220546078272 2201 29 1.43617504374334 2201 30 1.7194864989937315 2201 31 2.0423468520211614 2201 32 1.0857618312750574 2201 33 1.1445135377943245 2201 34 1.307163398557293 2201 35 1.0370574636022454 2201 36 0.961358932597669 2201 37 0.37245527756908814 2201 38 1.5912871600176288 2201 39 2.034635332551872 2201 40 2.5867478643362034 2201 41 2.294931990261844 2201 42 2.444610619654206 2201 43 2.3371198082359754 2201 44 2.358112151916984 2201 45 2.6074678670451448 2201 46 2.866194899754248 2201 47 2.988799416712375 2201 48 1.4275019068190886 2201 49 1.870880479981821 2201 50 2.272881491599712 2201 51 2.765285241352153 1101 1 0.7544168071430164 1101 2 0.7969625865628203 1101 3 0.9462651874632186 1101 4 0.8590192894143414 1101 5 -0.7967531958244578 1101 6 0.34865150488105456 1101 7 0.10485792678292682 1101 8 0.18893544794653394 1101 9 -0.37800750640122516 1101 10 0.7586127827405136 1101 11 0.6426857504221957 1101 12 0.0015868648026966525 1101 13 1.058465897280719 1101 14 -0.2738487343035274 1101 15 0.08274871037895792 1101 16 0.016931610492591176 1101 17 0.6563209767289706 1101 18 1.1663279294896327 1101 19 1.2899065289226854 1101 20 0.925182624517852 1101 21 1.4102732341957491 1101 22 1.1883494235899477 1101 23 1.2201943760447307 1101 24 1.0205390486794528 1101 25 0.4267519400252411 1101 26 0.8488209786468701 1101 27 1.1712203321536663 1101 28 -0.06654937396360339 1101 29 1.0879607580290553 1101 30 1.103415070422301 1101 31 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0.838848704672337 1208 41 1.5134193852198266 1208 42 0.016039191082775517 1208 43 -1.1838885951253673 1208 44 -0.3981903690914166 1208 45 0.8595687073812783 1208 46 0.5300604459727367 1208 47 0.5098078200737177 1208 48 1.0619556883316932 1208 49 1.0305443455280354 1208 50 2.298091575633329 1208 51 0.7988986867303041 1209 1 -1.0249949575628676 1209 2 0.8410802336216463 1209 3 0.9278828345220447 1209 4 0.8406369364731674 1209 5 0.10398209829318716 1209 6 -0.5778190833542425 1209 7 0.7261814561946949 1209 8 0.802906036181831 1209 9 0.22125719948112987 1209 10 0.5894951356816875 1209 11 -0.21393189663663037 1209 12 -1.0535601940208323 1209 13 -0.004034102719280952 1209 14 0.4062983245200016 1209 15 -1.0238689366798681 1209 16 0.4985492575514172 1209 17 -0.8988260820945584 1209 18 0.6222102824308067 1209 19 0.5104947642168014 1209 20 0.27812380098843903 1209 21 -1.0823738246277799 1209 22 -1.0616505764100523 1209 23 0.14666496428002773 1209 24 -2.9794609513205472 1209 25 -1.657806883504172 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1.0801857504221957 1214 12 1.2640868648026995 1214 13 1.495965897280719 1214 14 -0.2113487343035274 1214 15 1.1202487103789593 1214 16 0.8544316104925898 1214 17 0.7688209767289678 1214 18 0.1663279294896327 1214 19 0.9899065289226883 1214 20 0.5751826245178506 1214 21 0.6352732341957505 1214 22 0.43834942358994766 1214 23 0.5201943760447278 1214 24 1.0205390486794528 1214 25 1.189251940025244 1214 26 0.8738209786468687 1214 27 1.3462203321536634 1214 28 1.3209506260363995 1214 29 1.2504607580290568 1214 30 1.4909150704223038 1214 31 1.3852039948783101 1214 32 1.2286189741322033 1214 33 0.6373706806514718 1214 34 0.25716339855729586 1214 35 0.5227717493165329 1214 36 -0.9172124959737644 1214 37 0.6367409918548006 1214 38 0.5484300171604843 1214 39 1.25606390398044 1214 40 0.015319292907634008 1214 41 1.6163605616904135 1214 42 2.0160391910827755 1214 43 2.2514055225216936 1214 44 2.172397866202701 1214 45 1.4860392956165782 1214 46 0.39476632832568015 1214 47 1.324513702426657 1214 48 1.1560733353905164 1214 49 1.3923090514103933 1214 50 1.1157386344568607 1214 51 -0.02757190150499156 1215 1 0.18623498896119628 1215 2 -0.8166737770735466 1215 3 0.3155833692813985 1215 4 0.7283374712325212 1215 5 -0.3933441049153643 1215 6 -1.9468030405734922 1215 7 -1.514460255035253 1215 8 -1.4644736429625596 1215 9 -0.1450529609466784 1215 10 -1.1163872172594864 1215 11 -1.1925415223050777 1215 12 -1.2086404079245767 1215 13 -0.4585795572647342 1215 14 -0.029530552485347528 1215 15 -1.3661149259846752 1215 16 -1.6592047531437757 1215 17 -2.5993608414528495 1215 18 -1.310944797783094 1215 19 -0.8237298347136814 1215 20 -0.1202719209366947 1215 21 -0.9192722203497041 1215 22 -0.788923303682779 1215 23 1.0611034669538242 1215 24 -1.0703700422296407 1215 25 -1.3743844236111258 1215 26 -0.5261790213531299 1215 27 0.7734930594263929 1215 28 0.12095062603639661 1215 29 -0.8495392419709447 1215 30 -0.4363576568504257 1215 31 -0.13297782330351282 1215 32 0.1831644286776566 1215 33 0.24191613519692368 1215 34 0.0798906712845664 1215 35 -1.0408646143198368 1215 36 0.6691511403898716 1215 37 -0.23144082632701668 1215 38 1.1302481989786628 1215 39 1.196972994889535 1215 40 0.515319292907634 1215 41 0.5027241980540467 1215 42 1.288766463810049 1215 43 1.005950977067144 1215 44 0.34512513892997276 1215 45 -1.6912334316561513 1215 46 0.6584026919620456 1215 47 1.3654227933357532 1215 48 -0.41665393733675415 1215 49 1.169581778683117 1215 50 0.6884659071841313 1215 51 -0.33211735605044623 1212 1 -0.29558319285698076 1212 2 -1.3530374134371783 1212 3 -3.566234812536784 1212 4 -0.7534807105856558 1212 5 -2.1342531958244564 1212 6 -1.8513484951189483 1212 7 -3.6326420732170703 1212 8 -1.2235645520534675 1212 9 -1.3405075064012237 1212 10 -2.3163872172594893 1212 11 -1.3698142495778072 1212 12 -1.6359131351973062 1212 13 -2.204034102719284 1212 14 -0.7613487343035246 1212 15 -1.2797512896210392 1212 16 -4.89556838950741 1212 17 -4.981179023271032 1212 18 -1.3336720705103673 1212 19 -2.2600934710773117 1212 20 -1.9248173754821494 1212 21 -2.714726765804251 1212 22 -2.961650576410051 1212 23 -2.6298056239552707 1212 24 -1.67946095132055 1212 25 -0.8107480599747561 1212 26 -1.9261790213531285 1212 27 -3.003779667846331 1212 28 -3.529049373963602 1212 29 -3.9995392419709432 1212 30 -3.5090849295776962 1212 31 -1.5647960051216927 1212 32 -0.8213810258677938 1212 33 -3.0126293193485267 1212 34 -2.3428366014427056 1212 35 -4.5772282506834685 1212 36 -6.017212495973762 1212 37 -3.1132590081451994 1212 38 -2.50156998283952 1212 39 -1.49393609601956 1212 40 -3.234680707092366 1212 41 -2.483639438309588 1212 42 -4.683960808917227 1212 43 -3.398594477478312 1212 44 -4.177602133797301 1212 45 -3.4139607043834275 1212 46 -0.4052336716743241 1212 47 -1.2254862975733403 1212 48 -1.7939266646094794 1212 49 -4.457690948589608 1212 50 -3.584261365543142 1212 51 -2.92757190150499 1213 1 -0.27336097063476217 1213 2 -0.19377815417792021 1213 3 -1.5514199977219647 1213 4 -0.009036266141215776 1213 5 0.6268579152866565 1213 6 0.4134663196958712 1213 7 0.4080986675236673 1213 8 0.4042132257243125 1213 9 0.8465295306358129 1213 10 0.6243535234812541 1213 11 0.265370935607379 1213 12 0.47149427221010143 1213 13 -0.8188489175340976 1213 14 -0.7113487343035274 1213 15 0.010989451119698401 1213 16 -0.35112394506296596 1213 17 0.18178393969193252 1213 18 -0.8244128112511078 1213 19 0.07694356595972351 1213 20 0.3696270689622949 1213 21 0.479717678640192 1213 22 1.049460534701062 1213 23 -1.9927685869182312 1213 24 0.13165015979056705 1213 25 0.43369638446968395 1213 26 0.12196912679501537 1213 27 0.6017758877092234 1213 28 -2.5086790035932296 1213 29 -0.25694664937834943 1213 30 -0.8201960406888134 1213 31 0.33335214302645255 1213 32 -1.7343439888307586 1213 33 -1.45337006008927 1213 34 -0.21135511996122602 1213 35 -0.8994504729056914 1213 36 -0.5783236070848758 1213 37 -0.3391849340711204 1213 38 0.2632448319752996 1213 39 0.3097676076841438 1213 40 -0.8365325589442207 1213 41 -0.9484542531244031 1213 42 -0.9098867348431483 1213 43 -0.13377966266349262 1213 44 0.7020274958323256 1213 45 1.4434467030239801 1213 46 1.5540255875849383 1213 47 2.311550739463698 1213 48 -0.236519257202076 1213 49 -0.48361687451553337 1213 50 -0.3990761803579588 1213 51 -0.5257200496531382 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 5.0 18 1.0 19 2.0 20 4.0 21 5.0 22 11.0 23 28.0 24 39.0 25 35.0 26 62.0 27 101.0 28 117.0 29 159.0 30 187.0 31 212.0 32 264.0 33 337.0 34 412.0 35 519.0 36 678.0 37 873.0 38 1588.0 39 5907.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.909122411851335 32.50454821103699 13.28077622801698 11.30555314909469 2 7.942144465615798 35.47548934695999 31.933137017148795 24.64922917027542 3 19.66914948900052 43.07985449506322 30.192274380737917 7.058721635198337 4 8.574398059934177 30.72925688550147 34.50545643512905 26.190888619435306 5 10.73965009527109 41.57283907846873 28.564004850164558 19.12350597609562 6 23.2461458513771 45.36636064437901 10.349904728910445 21.03758877533345 7 7.690975229516715 54.28719902996709 18.872336739996538 19.149489000519658 8 18.638489520180148 39.94456954789538 21.011605750909407 20.40533518101507 9 17.04486402217218 36.177031006409145 21.62653732894509 25.151567642473584 10 12.757664992205092 42.92395634851896 21.193486921877707 23.124891737398233 11 15.234713320630522 41.061839598129225 19.18413303308505 24.519314048155206 12 17.41728737225013 45.66083492118483 26.04365148103239 10.878226225532652 13 21.115537848605577 38.610774294127836 26.294820717131472 13.978867140135112 14 16.914948900051964 38.98319764420579 31.59535769963624 12.506495756106013 15 17.59916854321843 39.35562099428373 31.22293434955829 11.822276112939546 16 7.7609354699003905 48.80034647033348 27.05067128627111 16.38804677349502 17 7.907500433050407 45.86869911657717 32.461458513771 13.762341936601421 18 7.613685578172369 38.30229536595929 43.89779125162408 10.186227804244261 19 8.825567296033258 44.19712454529707 40.68941624805127 6.287891910618396 20 6.131993764074138 38.41157110687684 42.89797332409492 12.558461804954096 21 3.2565390611467175 45.93798718170795 42.239736705352506 8.565737051792828 22 4.174605924129569 46.691494890005195 42.94127836480166 6.1926208210635725 23 3.4384202321150177 43.1837865927594 44.17114152087303 9.206651654252555 24 5.950112593105838 45.21912350597609 45.05456435129049 3.7761995496275764 25 5.101333795253767 45.81673306772908 43.11449852762861 5.967434609388532 26 5.257231941798025 45.19314048155205 44.81205612333275 4.737571453317166 27 4.850164559154686 46.8733760609735 44.552225879092326 3.7242335007794907 28 3.161267971591893 45.21912350597609 44.872683180322184 6.746925342109822 29 5.924129568681794 45.72146197817426 44.786073098908716 3.568335354235233 30 3.7418796015591163 46.842789086184496 44.98917280207882 4.426158510177566 31 4.460419192794041 47.99064611120735 43.798718170795084 3.7502165252035335 32 3.715572492638143 46.37969859691668 45.36636064437901 4.53836826606617 33 3.3777931751255847 47.70483284254287 45.5828858479127 3.334488134418846 34 4.94543564870951 48.181188290316996 43.27039667417287 3.6029793868006235 35 2.9793868006235926 48.42369651827473 43.3829897800104 5.213926901091287 36 3.2652000692880647 48.74415381950459 44.50892083838559 3.4817252728217563 37 2.8148276459379873 50.35510133379525 43.44361683699983 3.386454183266932 38 2.892776719210116 48.73549281136324 44.60419192794041 3.767538541486229 39 3.2565390611467175 48.3630694612853 43.0019054217911 5.378486055776892 40 3.048674865754374 48.45834055084012 44.71678503377793 3.7761995496275764 41 3.4644032565390614 48.54495063225359 42.34366880304867 5.64697730815867 42 5.317858998787458 48.11190022518621 42.681448120561235 3.888792655465096 43 3.5510133379525377 48.57093365667764 43.703447081240256 4.174605924129569 44 3.4384202321150177 50.34644032565391 42.46492291702754 3.7502165252035335 45 3.4470812402563658 48.66620474623246 43.67746405681621 4.209249956694959 46 5.2658929499393725 48.3630694612853 41.74605924129568 4.624978347479647 47 3.4817252728217563 48.813441884635374 41.58150008661008 6.123332755932791 48 3.6203014030833187 48.40637450199203 41.252381777238874 6.720942317685778 49 5.629655291875975 48.657543738091114 41.02719556556384 4.68560540446908 50 3.7848605577689245 48.73549281136324 42.4562619088862 5.023384721981639 51 3.594318378659276 48.969340031179634 40.827992378312835 6.608349211848259 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1610.0 1 859.0 2 108.0 3 194.5 4 281.0 5 275.5 6 270.0 7 525.5 8 781.0 9 586.5 10 392.0 11 533.5 12 675.0 13 1006.5 14 1338.0 15 1438.5 16 1539.0 17 1091.5 18 644.0 19 497.5 20 351.0 21 516.5 22 682.0 23 457.0 24 232.0 25 226.5 26 382.5 27 544.0 28 320.5 29 97.0 30 94.0 31 91.0 32 99.0 33 107.0 34 108.5 35 110.0 36 135.5 37 161.0 38 142.0 39 123.0 40 133.5 41 144.0 42 139.5 43 135.0 44 118.0 45 101.0 46 114.0 47 127.0 48 133.5 49 140.0 50 230.5 51 321.0 52 193.0 53 65.0 54 55.0 55 45.0 56 44.0 57 43.0 58 33.5 59 24.0 60 21.5 61 19.0 62 15.0 63 11.0 64 9.0 65 7.0 66 5.0 67 3.0 68 2.5 69 2.0 70 1.5 71 1.0 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02598302442404296 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.008661008141347654 17 0.0 18 0.008661008141347654 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.008661008141347654 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 11546.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.082279577342803 #Duplication Level Percentage of deduplicated Percentage of total 1 90.95303867403315 22.813095444309717 2 3.7292817679558015 1.870777758531093 3 1.0013812154696133 0.7535077082972458 4 0.6560773480662984 0.6582366187424216 5 0.3453038674033149 0.4330504070673827 6 0.20718232044198895 0.31179629308851553 7 0.2762430939226519 0.4850164559154686 8 0.24171270718232044 0.4850164559154686 9 0.10359116022099447 0.23384721981638662 >10 1.5538674033149171 8.297245799411051 >50 0.31077348066298344 5.5517062186038455 >100 0.48342541436464087 26.866447254460418 >500 0.10359116022099447 17.850337779317513 >1k 0.03453038674033149 13.389918586523471 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1546 13.389918586523471 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 983 8.513771002944742 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 549 4.754893469599861 No Hit GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 529 4.581673306772909 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 491 4.252554997401697 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 415 3.594318378659276 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 354 3.065996882037069 No Hit ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 233 2.018014896934003 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 230 1.9920318725099602 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 200 1.7322016282695307 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 199 1.7235406201281829 RNA PCR Primer, Index 27 (95% over 21bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 197 1.7062186038454874 No Hit GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 172 1.4896934003117963 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 139 1.2038801316473238 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA 127 1.0999480339511518 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 125 1.0826260176684566 No Hit CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 118 1.021998960679023 No Hit TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 102 0.8834228304174605 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA 93 0.8054737571453318 No Hit CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.7275246838732028 No Hit ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA 77 0.6668976268837693 No Hit GCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 76 0.6582366187424216 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 68 0.5889485536116403 No Hit GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 64 0.5543045210462498 No Hit GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 60 0.5196604884808591 No Hit CTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 60 0.5196604884808591 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 59 0.5109994803395115 No Hit TACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 50 0.4330504070673827 No Hit GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 48 0.4157283907846874 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 43 0.3724233500779491 No Hit GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42 0.3637623419366014 No Hit GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 42 0.3637623419366014 No Hit GTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAA 34 0.29447427680582017 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32 0.2771522605231249 No Hit CTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 28 0.2425082279577343 No Hit GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 27 0.23384721981638662 No Hit CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26 0.22518621167503897 No Hit CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 25 0.21652520353369134 No Hit TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 24 0.2078641953923437 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24 0.2078641953923437 No Hit AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 24 0.2078641953923437 No Hit GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22 0.19054217910964838 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21 0.1818811709683007 No Hit GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21 0.1818811709683007 No Hit ACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21 0.1818811709683007 No Hit GATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 21 0.1818811709683007 No Hit TACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 20 0.17322016282695307 No Hit TTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.1645591546856054 No Hit ACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.1645591546856054 No Hit CTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 19 0.1645591546856054 No Hit TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT 19 0.1645591546856054 RNA PCR Primer, Index 38 (95% over 21bp) TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.15589814654425777 No Hit CCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 18 0.15589814654425777 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA 16 0.13857613026156246 No Hit AAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAA 16 0.13857613026156246 No Hit GCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.13857613026156246 No Hit GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 16 0.13857613026156246 No Hit ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA 15 0.12991512212021478 No Hit AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15 0.12991512212021478 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAA 15 0.12991512212021478 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAA 15 0.12991512212021478 No Hit CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.11259310583751948 No Hit AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAA 13 0.11259310583751948 No Hit ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13 0.11259310583751948 No Hit CGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12 0.10393209769617184 No Hit AAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAA 12 0.10393209769617184 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.008661008141347654 10 0.0 0.0 0.008661008141347654 11 0.0 0.0 0.008661008141347654 12 0.0 0.0 0.008661008141347654 13 0.0 0.0 0.008661008141347654 14 0.0 0.0 0.008661008141347654 15 0.0 0.0 0.008661008141347654 16 0.0 0.0 0.008661008141347654 17 0.0 0.0 0.008661008141347654 18 0.0 0.0 0.008661008141347654 19 0.0 0.0 0.008661008141347654 20 0.0 0.0 0.008661008141347654 21 0.0 0.0 0.008661008141347654 22 0.0 0.0 0.017322016282695307 23 0.0 0.0 0.025983024424042957 24 0.0 0.0 0.04330504070673826 25 0.0 0.0 0.051966048848085915 26 0.0 0.0 0.06928806513078123 27 0.0 0.0 0.08661008141347652 28 0.0 0.0 0.1299151221202148 29 0.0 0.0 0.199203187250996 30 0.0 0.0 0.32045730122986316 31 0.0 0.0 0.424389398926035 32 0.0 0.0 0.5196604884808592 33 0.0 0.0 0.6149315780356833 34 0.0 0.0 0.7361856920145505 35 0.0 0.0 0.8314567815693746 36 0.0 0.0 0.9700329118309371 37 0.0 0.0 1.1086090420924997 38 0.0 0.0 1.2645071886367574 39 0.0 0.0 1.4290663433223627 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCCCAC 25 3.6545047E-5 45.0 17 GTATGCC 25 3.6545047E-5 45.0 42 ATCTCGT 25 3.6545047E-5 45.0 37 GCCCACG 25 3.6545047E-5 45.0 18 TGCCGTC 25 3.6545047E-5 45.0 45 CCACGAG 25 3.6545047E-5 45.0 20 TATGCCG 25 3.6545047E-5 45.0 43 GAGCCCA 25 3.6545047E-5 45.0 16 CACGAGA 25 3.6545047E-5 45.0 21 CCCACGA 25 3.6545047E-5 45.0 19 GCTATCT 25 3.6545047E-5 45.0 34 TCTCGTA 25 3.6545047E-5 45.0 38 ATTGCTA 25 3.6545047E-5 45.0 31 ACGAGAC 25 3.6545047E-5 45.0 22 TATCTCG 25 3.6545047E-5 45.0 36 CGAATTG 25 3.6545047E-5 45.0 28 AGACGAA 25 3.6545047E-5 45.0 25 ATGCCGT 25 3.6545047E-5 45.0 44 CGTATGC 25 3.6545047E-5 45.0 41 GAATTGC 25 3.6545047E-5 45.0 29 >>END_MODULE