FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 48h_1.3410000000682f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 48h_1.3410000000682f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11546
Sequences flagged as poor quality0
Sequence length51
%GC17

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA154613.389918586523471No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9838.513771002944742No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5494.754893469599861No Hit
GCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5294.581673306772909No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4914.252554997401697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4153.594318378659276No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3543.065996882037069No Hit
ATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2332.018014896934003No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2301.9920318725099602No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2001.7322016282695307No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC1991.7235406201281829RNA PCR Primer, Index 27 (95% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1971.7062186038454874No Hit
GATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1721.4896934003117963No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1391.2038801316473238No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAA1271.0999480339511518No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1251.0826260176684566No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1181.021998960679023No Hit
TGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1020.8834228304174605No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAA930.8054737571453318No Hit
CCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA840.7275246838732028No Hit
ACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAA770.6668976268837693No Hit
GCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA760.6582366187424216No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.5889485536116403No Hit
GTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.5543045210462498No Hit
GCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.5196604884808591No Hit
CTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA600.5196604884808591No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT590.5109994803395115No Hit
TACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA500.4330504070673827No Hit
GCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.4157283907846874No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.3724233500779491No Hit
GCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.3637623419366014No Hit
GCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.3637623419366014No Hit
GTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAA340.29447427680582017No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320.2771522605231249No Hit
CTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.2425082279577343No Hit
GCGTTGATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.23384721981638662No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.22518621167503897No Hit
CCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.21652520353369134No Hit
TACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.2078641953923437No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.2078641953923437No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT240.2078641953923437No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.19054217910964838No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.1818811709683007No Hit
GATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.1818811709683007No Hit
ACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.1818811709683007No Hit
GATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.1818811709683007No Hit
TACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.17322016282695307No Hit
TTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA190.1645591546856054No Hit
ACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAA190.1645591546856054No Hit
CTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAA190.1645591546856054No Hit
TTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTCT190.1645591546856054RNA PCR Primer, Index 38 (95% over 21bp)
TGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.15589814654425777No Hit
CCACTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.15589814654425777No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAA160.13857613026156246No Hit
AAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAAAA160.13857613026156246No Hit
GCTTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.13857613026156246No Hit
GATACCACTGCTTCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.13857613026156246No Hit
ACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAA150.12991512212021478No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.12991512212021478No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAA150.12991512212021478No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCGAAAAAAAAAAAAAAAAAAA150.12991512212021478No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.11259310583751948No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAA130.11259310583751948No Hit
ATACCACTGCTTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.11259310583751948No Hit
CGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.10393209769617184No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAA120.10393209769617184No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCAC253.6545047E-545.017
GTATGCC253.6545047E-545.042
ATCTCGT253.6545047E-545.037
GCCCACG253.6545047E-545.018
TGCCGTC253.6545047E-545.045
CCACGAG253.6545047E-545.020
TATGCCG253.6545047E-545.043
GAGCCCA253.6545047E-545.016
CACGAGA253.6545047E-545.021
CCCACGA253.6545047E-545.019
GCTATCT253.6545047E-545.034
TCTCGTA253.6545047E-545.038
ATTGCTA253.6545047E-545.031
ACGAGAC253.6545047E-545.022
TATCTCG253.6545047E-545.036
CGAATTG253.6545047E-545.028
AGACGAA253.6545047E-545.025
ATGCCGT253.6545047E-545.044
CGTATGC253.6545047E-545.041
GAATTGC253.6545047E-545.029
Produced by FastQC (version 0.11.2)