H23NVBCXX l02n01 36h_96.34100000006218.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 36h_96.34100000006218.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2847
Sequences flagged as poor quality	0
Sequence length	51
%GC	52

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	2205	77.44994731296102	No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT	132	4.636459430979979	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	30	1.053740779768177	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	28	0.9834913944502985	No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT	26	0.91324200913242	No Hit
ATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTTC	14	0.49174569722514927	Illumina PCR Primer Index 7 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG	14	0.49174569722514927	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	10	0.3512469265893923	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	8	0.2809975412715139	RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	7	0.24587284861257463	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	7	0.24587284861257463	No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATTTCGTATGCCGTC	7	0.24587284861257463	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTGATTAAATCTCGTATGCCGTC	6	0.21074815595363539	No Hit
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC	6	0.21074815595363539	No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC	5	0.17562346329469616	No Hit
CTTATACACATCTACGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC	5	0.17562346329469616	No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATATCGTATGCCGTC	4	0.14049877063575694	No Hit
TATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTT	4	0.14049877063575694	Illumina PCR Primer Index 7 (95% over 21bp)
CTTATACACCATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT	4	0.14049877063575694	No Hit
CGGAGATGTGTATAAGAGACAGGCGTCAGATGTGTATAAGAGACAGCTTAT	4	0.14049877063575694	No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT	4	0.14049877063575694	No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGGCTAATCTCGTATGCCGTC	3	0.10537407797681769	No Hit
ACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTTCTG	3	0.10537407797681769	Illumina PCR Primer Index 7 (95% over 24bp)
CTTATACACATCTCCGAGCCCACGAGACACTGGATAATCTCGTATGCCGTC	3	0.10537407797681769	No Hit
CTTATACACATCTCCGAGCCCACGAGACCTTGCAGAATCTCGTATGCCGTC	3	0.10537407797681769	No Hit
CGTCAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGAC	3	0.10537407797681769	No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGAATAATCTCGTATGCCGTC	3	0.10537407797681769	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TGCCGTC	235	43.085106	45
CTTATAC	245	42.2449	1
TATACAC	250	41.399998	3
TTATACA	250	41.399998	2
ATGCCGT	245	41.32653	44
GTATGCC	250	40.499996	42
ATCTCGT	250	40.499996	37
CACATCT	250	40.499996	7
TATGCCG	250	40.499996	43
ACATCTC	250	40.499996	8
TACACAT	250	40.499996	5
ACACATC	250	40.499996	6
TCTCGTA	250	40.499996	38
ACGAGAC	250	40.499996	22
CGTATGC	250	40.499996	41
TCGTATG	250	40.499996	40
ATACACA	250	40.499996	4
CTCGTAT	250	40.499996	39
AATCTCG	245	40.408165	36
TAATCTC	245	40.408165	35

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content