FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_95.3410000000620b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_95.3410000000620b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4394
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC309170.34592626308603No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT2415.484751934456076No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGT851.9344560764679108No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCG330.7510241238051889No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC320.7282658170232135No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC260.591715976331361No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC260.591715976331361No Hit
ATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTTC150.34137460172963136Illumina PCR Primer Index 7 (95% over 22bp)
CGGAGATGTGTATAAGAGACAGCTTATACACATCTCCGAGCCCACGAGACC140.31861629494765586No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC120.27309968138370505No Hit
TATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCTT70.15930814747382793Illumina PCR Primer Index 7 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATATCGTATGCCGTC60.13654984069185253No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC60.13654984069185253RNA PCR Primer, Index 27 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGCC50.11379153390987709No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC50.11379153390987709No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTGAATAATCTCGTATGCCGTC50.11379153390987709No Hit
TTTCAAAACTGCTGTGTCAAAAGGAAGGTTCAACTCTGTTACTTGAGTACA50.11379153390987709No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC50.11379153390987709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT301.7077964E-644.99999645
TGCCGTC3550.041.19718645
CTTATAC3600.040.01
ATGCCGT3700.039.52702744
GTATGCC3800.038.48684342
ATCTCGT3800.038.48684337
TATGCCG3800.038.48684343
TCTCGTA3800.038.48684338
ACGAGAC3800.038.48684322
CGTATGC3800.038.48684341
TCGTATG3800.038.48684340
CTCGTAT3800.038.48684339
AATCTCG3750.038.436
CGAGACC3700.038.3108123
GATAATC3650.038.21917733
ATAATCT3650.038.21917734
GGATAAT3650.038.21917732
CTGGATA3650.038.21917730
TAATCTC3650.038.21917735
ACCCTGG3650.038.21917727
Produced by FastQC (version 0.11.2)