##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_94.341000000061fd.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 100338 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.22459088281608 38.0 38.0 38.0 32.0 38.0 2 36.261286850445494 38.0 38.0 38.0 32.0 38.0 3 36.2526460563296 38.0 38.0 38.0 32.0 38.0 4 36.35497020072156 38.0 38.0 38.0 32.0 38.0 5 36.37385636548466 38.0 38.0 38.0 32.0 38.0 6 38.281677928601326 40.0 38.0 40.0 38.0 40.0 7 38.17419123363033 40.0 38.0 40.0 38.0 40.0 8 38.147302118838326 40.0 38.0 40.0 38.0 40.0 9 38.174480256732245 40.0 38.0 40.0 38.0 40.0 10 38.112130997229364 40.0 38.0 40.0 38.0 40.0 11 38.104098148258885 40.0 38.0 40.0 38.0 40.0 12 37.99082102493572 40.0 38.0 40.0 32.0 40.0 13 38.038679264087385 40.0 38.0 40.0 32.0 40.0 14 37.972283681157684 40.0 38.0 40.0 32.0 40.0 15 37.99476768522394 40.0 38.0 40.0 32.0 40.0 16 37.96276585142219 40.0 38.0 40.0 32.0 40.0 17 37.97879168410772 40.0 38.0 40.0 32.0 40.0 18 38.01886623213538 40.0 38.0 40.0 32.0 40.0 19 38.04520719966513 40.0 38.0 40.0 32.0 40.0 20 38.032898802049075 40.0 38.0 40.0 32.0 40.0 21 38.051306583746936 40.0 38.0 40.0 32.0 40.0 22 38.03449341226654 40.0 38.0 40.0 32.0 40.0 23 38.0304769877813 40.0 38.0 40.0 32.0 40.0 24 38.02735753154338 40.0 38.0 40.0 32.0 40.0 25 38.03246028423927 40.0 38.0 40.0 32.0 40.0 26 37.96775897466563 40.0 38.0 40.0 32.0 40.0 27 37.9293587673663 40.0 38.0 40.0 32.0 40.0 28 37.89982857940162 40.0 38.0 40.0 32.0 40.0 29 37.88520799697024 40.0 38.0 40.0 32.0 40.0 30 37.874733401104265 40.0 38.0 40.0 32.0 40.0 31 37.84813330941418 40.0 38.0 40.0 32.0 40.0 32 37.815613227291756 40.0 38.0 40.0 32.0 40.0 33 37.76531324124459 40.0 38.0 40.0 32.0 40.0 34 37.748669497099804 40.0 38.0 40.0 32.0 40.0 35 37.758227192090736 40.0 38.0 40.0 32.0 40.0 36 37.687785285734215 40.0 38.0 40.0 32.0 40.0 37 37.676453586876356 40.0 38.0 40.0 32.0 40.0 38 37.63809324483247 40.0 38.0 40.0 32.0 40.0 39 37.55675815742789 40.0 38.0 40.0 32.0 40.0 40 37.501126193466085 40.0 38.0 40.0 32.0 40.0 41 37.44784627957504 40.0 38.0 40.0 32.0 40.0 42 37.37550080727142 40.0 38.0 40.0 32.0 40.0 43 37.26922003627738 40.0 38.0 40.0 32.0 40.0 44 37.16425481871275 40.0 38.0 40.0 32.0 40.0 45 37.0694951065399 40.0 38.0 40.0 32.0 40.0 46 36.89995814148179 40.0 38.0 40.0 32.0 40.0 47 36.804261595806175 40.0 38.0 40.0 32.0 40.0 48 36.697472542805315 40.0 38.0 40.0 32.0 40.0 49 36.598905698738264 40.0 38.0 40.0 27.0 40.0 50 36.57093025573561 40.0 38.0 40.0 27.0 40.0 51 36.099224620781754 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.5382511340973508 2201 2 0.7144514053980231 2201 3 0.5988111663157767 2201 4 0.4426397054878777 2201 5 0.7333516047725155 2201 6 0.5075596867060952 2201 7 0.6078850617704745 2201 8 0.7833920430860957 2201 9 0.6772509638653403 2201 10 0.4813425814712886 2201 11 1.0095723489640065 2201 12 0.9567315653855459 2201 13 0.5076524682403161 2201 14 0.30885301776135776 2201 15 0.8222595879654762 2201 16 1.016811447448596 2201 17 0.6391396399424494 2201 18 0.42398922987731424 2201 19 1.1579439581519537 2201 20 0.9707047383136782 2201 21 0.5844737410819292 2201 22 0.9034627472884438 2201 23 0.6474423883474927 2201 24 0.6300991282951642 2201 25 0.7272908510132368 2201 26 0.6968035203043712 2201 27 0.5616668024895191 2201 28 0.8012506967156909 2201 29 0.35621177132510695 2201 30 0.8273882047302052 2201 31 1.2322919894770692 2201 32 0.564320251330436 2201 33 0.13101934893644085 2201 34 0.3626963157971872 2201 35 0.6465468378929344 2201 36 0.6365948470441509 2201 37 0.8413027075267152 2201 38 0.9723778798983744 2201 39 0.584915706889511 2201 40 0.9636375192244273 2201 41 0.9148115778337242 2201 42 0.4851783019719065 2201 43 0.07071241234436343 2201 44 0.35270285182834726 2201 45 0.21962422789229663 2201 46 0.13343584828177057 2201 47 0.3648337216118662 2201 48 0.44906290082217026 2201 49 0.2286291231102595 2201 50 0.2516626619482949 2201 51 1.0651935774079675 1101 1 0.11212091562449444 1101 2 0.280317725969077 1101 3 -0.0064235297690160564 1101 4 0.03457135160511626 1101 5 0.1071348623974373 1101 6 -0.45826337191384425 1101 7 -0.06851554389684367 1101 8 0.21839096153731674 1101 9 -0.12363590613249187 1101 10 -0.23907705945451596 1101 11 0.3390121066970835 1101 12 0.22722691472580436 1101 13 0.1517147654499169 1101 14 -0.5092304778042944 1101 15 -0.16530260108924466 1101 16 0.13340240570081363 1101 17 0.6787243252117534 1101 18 -0.5742802920781216 1101 19 -0.058473952295805987 1101 20 0.21535107186331715 1101 21 0.27558341012800724 1101 22 -0.2324879340872883 1101 23 -0.011869746629791678 1101 24 0.08099681378077861 1101 25 -0.28860791602115654 1101 26 -0.04816727787862618 1101 27 0.06956210856782263 1101 28 -0.662409264348554 1101 29 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0.6148231670150892 2202 15 0.792408841696826 2202 16 0.5317368205829212 2202 17 0.004811281733495321 2202 18 0.6627952000265722 2202 19 0.21764545068926822 2202 20 0.4259286189106888 2202 21 0.4725334425744663 2202 22 0.918388120422776 2202 23 0.4608752241683902 2202 24 0.6450245014294893 2202 25 0.39146995549084806 2202 26 0.45799755015511323 2202 27 0.7631593398029537 2202 28 0.7191611444768853 2202 29 0.5129281892355522 2202 30 0.49156730920781655 2202 31 0.03826213873080064 2202 32 0.40760383341999074 2202 33 -0.30927915852624466 2202 34 -0.15969174390431107 2202 35 0.9450543005794998 2202 36 0.8828635037605679 2202 37 0.2816012149894078 2202 38 0.8455122082565865 2202 39 1.0177515277850304 2202 40 1.2099061759408443 2202 41 1.1163041151471589 2202 42 1.55234248107638 2202 43 1.3095183824936143 2202 44 0.9422550906343119 2202 45 1.025594377146028 2202 46 0.7901522661922158 2202 47 0.41707252758201463 2202 48 0.8072718560460572 2202 49 0.6987783768416023 2202 50 0.759125348515461 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15866.0 39 48196.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.56215666530412 19.794024106955924 13.974656789926524 27.669162437813426 2 14.719395636704308 21.68093524821352 40.04205826365149 23.55761085143068 3 18.666905858199286 24.31082939663936 30.548745241085136 26.473519504076222 4 13.098726305088801 19.3575714086388 35.461141342263154 32.082560944009245 5 14.045526121708624 36.36408937790269 34.48244932129403 15.107935179094659 6 30.69026689788515 38.830752058043814 16.209212860531405 14.269768183539636 7 26.259243756104368 33.104108114572746 20.134943889652973 20.501704239669916 8 23.866331798521 37.133488807829536 19.737287966672646 19.262891426976818 9 23.78261476210409 18.570232613765473 20.719966513185433 36.927186110945 10 15.361079551117223 30.768004145986566 31.344057087045783 22.526859215850425 11 31.389218334213698 25.14027726561488 24.77749982558777 18.693004574583654 12 22.590643624549024 26.082840000797304 29.313918953935698 22.012597420717974 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20.223644082999463 26 21.64106959546329 27.96675204560631 30.421479613701823 19.970698745228578 27 19.873826466543086 29.539157647152624 30.149096055332976 20.437919830971314 28 19.222029540154278 27.905678805636946 30.129163427614664 22.743128226594113 29 19.845920787737448 27.913651856724268 30.01156092407662 22.22886643146166 30 20.05760644235369 27.2982777866369 30.355007175889014 22.289108595120396 31 20.34622974346708 27.427295740397454 31.62012398094441 20.606350535191055 32 21.680719169208075 27.764157148836933 29.749446869580815 20.805676812374173 33 21.70962147939963 27.892722597620047 30.03448344595268 20.363172477027646 34 19.982459287607885 29.47537323845403 30.560704817716122 19.98146265622197 35 20.114014630548745 28.010325101158084 31.57926209412187 20.2963981741713 36 19.919671510295203 29.31491558532161 30.22583667204848 20.539576232334706 37 19.70639239370926 28.771751479997608 30.376328011321736 21.145528114971395 38 19.272857740835974 26.927983416053735 32.10747672865714 21.691682114453148 39 19.705395762323345 26.654906416312862 30.78096035400347 22.85873746736032 40 19.92963782415436 26.382826047957902 32.87089637026849 20.816639757619246 41 20.15786641152903 25.99812633299448 31.027128306324624 22.816878949151867 42 21.24917777910662 26.422691303394526 31.217484901034503 21.11064601646435 43 19.21903964599653 26.326018058960717 32.43237856046562 22.02256373457713 44 18.832346668261277 28.4518328051187 31.079949769778146 21.635870756841875 45 19.305746576571188 26.27020670134944 32.42639877215014 21.997647949929238 46 20.983077199067154 26.155594091969142 30.498913671789353 22.36241503717435 47 19.092467459985247 26.470529609918476 30.266698558871013 24.17030437122526 48 18.591161872869698 26.98578803643684 30.194941099085092 24.228108991608362 49 20.58043811915725 26.67384241264526 30.460045047738642 22.285674420458847 50 18.05597081863302 27.32264944487632 31.665969024696526 22.955410711794137 51 18.255210150199837 27.15756530752594 29.648271256715137 24.938953285559087 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 305.0 1 175.0 2 45.0 3 63.5 4 82.0 5 93.5 6 105.0 7 122.0 8 139.0 9 177.5 10 216.0 11 350.0 12 484.0 13 763.0 14 1042.0 15 1259.0 16 1476.0 17 1401.5 18 1327.0 19 1266.0 20 1205.0 21 1153.5 22 1102.0 23 1078.0 24 1054.0 25 1084.5 26 1239.5 27 1364.0 28 1673.0 29 1982.0 30 2534.0 31 3086.0 32 3640.0 33 4194.0 34 4737.0 35 5280.0 36 5491.0 37 5702.0 38 5938.5 39 6175.0 40 6294.5 41 6414.0 42 6361.5 43 6309.0 44 6388.5 45 6468.0 46 6475.0 47 6482.0 48 6310.0 49 6138.0 50 6212.5 51 6287.0 52 6759.5 53 7232.0 54 5910.0 55 4588.0 56 4095.5 57 3603.0 58 3177.0 59 2751.0 60 2439.5 61 2128.0 62 1822.5 63 1517.0 64 1304.0 65 1091.0 66 923.5 67 756.0 68 578.0 69 400.0 70 329.0 71 258.0 72 213.0 73 168.0 74 144.5 75 96.5 76 72.0 77 54.0 78 36.0 79 31.0 80 26.0 81 16.5 82 7.0 83 6.0 84 5.0 85 3.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03488209850704618 2 9.966313859156052E-4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 9.966313859156052E-4 12 0.0 13 0.0 14 0.0 15 0.0 16 0.002989894157746816 17 0.0 18 0.004983156929578026 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 9.966313859156052E-4 27 0.0 28 0.0 29 0.0 30 0.0019932627718312105 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.004983156929578026 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 100338.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 89.65099737919347 #Duplication Level Percentage of deduplicated Percentage of total 1 95.23983770531056 85.38346440513607 2 3.762184817604047 6.745672424461237 3 0.56847056084321 1.5289185828090979 4 0.16194129116294365 0.5807279307852906 5 0.06904455155543766 0.3094956455272065 6 0.05717405680267469 0.3075426729944668 7 0.03641716759385947 0.2285384777560321 8 0.022086862124657238 0.15840873747618026 9 0.008159868305477906 0.06583862988620671 >10 0.06468573783123323 0.9693766991382117 >50 0.0022229435224928916 0.17237358868584648 >100 0.005553176328285043 1.2336641782221656 >500 0.0011106305075582155 0.5068068855567813 >1k 0.0011106305075582155 1.8091711415651701 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC 1817 1.8108792282086548 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 509 0.507285375431043 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 306 0.3049692040901752 No Hit TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT 302 0.3009826785465128 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 287 0.28603320775777874 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 221 0.22025553628734876 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 123 0.12258566046761946 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 9.966313859156052E-4 4 0.0 0.0 9.966313859156052E-4 5 0.0 0.0 9.966313859156052E-4 6 0.0 0.0 9.966313859156052E-4 7 0.0 0.0 9.966313859156052E-4 8 0.0 0.0 9.966313859156052E-4 9 0.0 0.0 9.966313859156052E-4 10 0.0 0.0 9.966313859156052E-4 11 0.0 0.0 9.966313859156052E-4 12 0.0 0.0 9.966313859156052E-4 13 0.0 0.0 9.966313859156052E-4 14 0.0 0.0 9.966313859156052E-4 15 0.0 0.0 9.966313859156052E-4 16 0.0 0.0 9.966313859156052E-4 17 0.0 0.0 9.966313859156052E-4 18 0.0 0.0 0.0019932627718312105 19 0.0 0.0 0.0019932627718312105 20 0.0 0.0 0.002989894157746816 21 0.0 0.0 0.003986525543662421 22 0.0 0.0 0.004983156929578026 23 0.0 0.0 0.008969682473240446 24 0.0 0.0 0.010962945245071657 25 0.0 0.0 0.01494947078873408 26 0.0 0.0 0.02292252187605892 27 0.0 0.0 0.027905678805636946 28 0.0 0.0 0.046841675138033445 29 0.0 0.0 0.09368335027606689 30 0.0 0.0 0.15846439036058124 31 0.0 0.0 0.4425043353465287 32 0.0 0.0 0.7883354262592438 33 0.0 0.0 1.1760250353804143 34 0.0 0.0 1.6095596882537024 35 0.0 0.0 2.0361179214255816 36 0.0 0.0 2.5862584464509957 37 0.0 0.0 3.327752197572206 38 0.0 0.0 4.188841715003289 39 0.0 0.0 5.182483206761147 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCCACG 230 0.0 39.130005 18 CGAGACC 230 0.0 39.130005 23 ACGAGAC 230 0.0 39.130005 22 GGATAAT 225 0.0 38.99957 32 CACGAGA 235 0.0 38.297447 21 CCCACGA 235 0.0 38.297447 19 TCTCGTA 230 0.0 38.151752 38 TCGTATG 230 0.0 38.151752 40 CGAGCCC 240 0.0 37.499584 15 CCTGGAT 240 0.0 37.499584 29 CGTATGC 235 0.0 37.34001 41 TATGCCG 235 0.0 37.34001 43 ATGCCGT 230 0.0 37.173504 44 TGCCGTC 230 0.0 37.173504 45 ATCTCCG 245 0.0 36.734287 10 CTCCGAG 245 0.0 36.734287 12 GAGCCCA 245 0.0 36.734287 16 CTGGATA 245 0.0 36.734287 30 GATAATC 240 0.0 36.56209 33 CTCGTAT 240 0.0 36.56209 39 >>END_MODULE