FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_94.341000000061fd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_94.341000000061fd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100338
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC18171.8108792282086548No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5090.507285375431043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3060.3049692040901752No Hit
TTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTCT3020.3009826785465128No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2870.28603320775777874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2210.22025553628734876No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1230.12258566046761946No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2300.039.13000518
CGAGACC2300.039.13000523
ACGAGAC2300.039.13000522
GGATAAT2250.038.9995732
CACGAGA2350.038.29744721
CCCACGA2350.038.29744719
TCTCGTA2300.038.15175238
TCGTATG2300.038.15175240
CGAGCCC2400.037.49958415
CCTGGAT2400.037.49958429
CGTATGC2350.037.3400141
TATGCCG2350.037.3400143
ATGCCGT2300.037.17350444
TGCCGTC2300.037.17350445
ATCTCCG2450.036.73428710
CTCCGAG2450.036.73428712
GAGCCCA2450.036.73428716
CTGGATA2450.036.73428730
GATAATC2400.036.5620933
CTCGTAT2400.036.5620939