FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_92.341000000061d3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_92.341000000061d3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304313
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC21900.719653777525114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.10482628083584994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCCC2150.037.6738715
TATGCCG2200.036.81764243
AATCTCG2250.035.99947436
ATGCCGT2200.035.7949344
TCGTATG2300.035.21687740
CGTATGC2350.034.46758341
ACGAGAC2350.034.46758322
TCTCGTA2400.033.74950838
TGCCGTC2350.033.51014745
CCGAGCC2450.033.0607414
ATCTCGT2500.032.39952537
GCCCACG2500.032.39952518
CTCGTAT2500.032.39952539
CACGAGA2500.032.39952521
CGAGACC2450.032.14238723
TCCGAGC2550.031.76424213
CCCACGA2550.031.76424219
CCACGAG2550.031.76424220
CTCCGAG2600.031.1533912
TCTCCGA2700.030.83288211
Produced by FastQC (version 0.11.2)