FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_91.341000000061c6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_91.341000000061c6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438025
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTGGATAATCTCGTATGCCGTC16170.3691570115860967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT2200.040.9080539
TATGCCG2250.038.9990143
TGCCGTC2250.038.9990145
TCTCGTA2350.038.296938
CGAGCCC2350.038.296915
CGTATGC2450.037.65210341
AATCTCG2400.037.4990536
ATGCCGT2500.035.9990944
ACGAGAC2500.035.9990922
CCCACGA2550.035.29322419
CGAGACC2500.035.0991123
TCGTATG2800.034.55269640
GATAATC2550.034.41089233
CACGAGA2650.033.96140321
GCCCACG2750.032.7264418
TCTCCGA2800.032.14571411
ATCTCGT2800.032.14204437
GGATAAT2750.031.9082832
GTATGCC2850.031.57814842
ATCTCCG2900.031.03724110