##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_9.34100000005bf7.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2611 Sequences flagged as poor quality 0 Sequence length 51 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.083109919571044 38.0 38.0 38.0 32.0 38.0 2 36.15358100344695 38.0 38.0 38.0 32.0 38.0 3 36.03715051704328 38.0 38.0 38.0 32.0 38.0 4 36.31328992723095 38.0 38.0 38.0 32.0 38.0 5 36.331290693220986 38.0 38.0 38.0 32.0 38.0 6 38.18843355036385 40.0 38.0 40.0 38.0 40.0 7 37.872462657985444 40.0 38.0 40.0 32.0 40.0 8 37.9662964381463 40.0 38.0 40.0 32.0 40.0 9 38.07621600919188 40.0 38.0 40.0 38.0 40.0 10 37.933741861355806 40.0 38.0 40.0 32.0 40.0 11 37.97127537342015 40.0 38.0 40.0 32.0 40.0 12 37.793182688625045 40.0 38.0 40.0 32.0 40.0 13 37.779777862887784 40.0 38.0 40.0 32.0 40.0 14 37.67905017234776 40.0 38.0 40.0 32.0 40.0 15 37.69322098812715 40.0 38.0 40.0 32.0 40.0 16 37.37725009574876 40.0 38.0 40.0 32.0 40.0 17 37.17387973956338 40.0 38.0 40.0 32.0 40.0 18 37.26081960934508 40.0 38.0 40.0 32.0 40.0 19 37.47031788586748 40.0 38.0 40.0 32.0 40.0 20 37.547299885101495 40.0 38.0 40.0 32.0 40.0 21 37.53504404442742 40.0 38.0 40.0 32.0 40.0 22 37.65760245116814 40.0 38.0 40.0 32.0 40.0 23 37.73420145538108 40.0 38.0 40.0 32.0 40.0 24 37.52623515894293 40.0 38.0 40.0 32.0 40.0 25 37.25354270394485 40.0 38.0 40.0 32.0 40.0 26 37.11145155112983 40.0 38.0 40.0 32.0 40.0 27 36.928379931060896 40.0 38.0 40.0 32.0 40.0 28 37.163155878973576 40.0 38.0 40.0 32.0 40.0 29 37.22788203753351 40.0 38.0 40.0 32.0 40.0 30 37.27154346993489 40.0 38.0 40.0 32.0 40.0 31 37.358483339716585 40.0 38.0 40.0 32.0 40.0 32 37.301800076599 40.0 38.0 40.0 32.0 40.0 33 37.150900038299504 40.0 38.0 40.0 32.0 40.0 34 37.091918805055535 40.0 38.0 40.0 32.0 40.0 35 37.011106855610876 40.0 38.0 40.0 32.0 40.0 36 37.063577173496746 40.0 38.0 40.0 32.0 40.0 37 36.749904251244736 40.0 38.0 40.0 32.0 40.0 38 36.74875526618154 40.0 38.0 40.0 32.0 40.0 39 36.61930294906166 38.0 38.0 40.0 32.0 40.0 40 36.597855227882036 38.0 38.0 40.0 32.0 40.0 41 36.62083492914592 40.0 38.0 40.0 27.0 40.0 42 36.459594025277674 38.0 38.0 40.0 27.0 40.0 43 36.72769054002298 40.0 38.0 40.0 32.0 40.0 44 36.36307927996936 40.0 38.0 40.0 27.0 40.0 45 36.55802374569131 40.0 38.0 40.0 27.0 40.0 46 36.12677135197242 38.0 38.0 40.0 27.0 40.0 47 36.076599004212945 38.0 38.0 40.0 27.0 40.0 48 36.0727690540023 38.0 38.0 40.0 27.0 40.0 49 35.72386058981233 38.0 38.0 40.0 27.0 40.0 50 35.65645346610494 38.0 38.0 40.0 27.0 40.0 51 35.280735350440445 38.0 38.0 40.0 27.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2201 1 1.683110119047619 2201 2 -0.8764880952380878 2201 3 0.43846726190475493 2201 4 1.3853794642857196 2201 5 -1.5816964285714192 2201 6 1.358891369047626 2201 7 1.9278273809523938 2201 8 0.7146949404762069 2201 9 -0.17518601190474925 2201 10 -0.7511532738095212 2201 11 0.5111607142857153 2201 12 0.956101190476204 2201 13 1.891815476190473 2201 14 1.0111235119047635 2201 15 1.641852678571425 2201 16 1.942410714285721 2201 17 0.8296130952380949 2201 18 -0.7872767857142833 2201 19 0.7194940476190581 2201 20 1.1840401785714363 2201 21 0.688504464285721 2201 22 0.8988839285714363 2201 23 1.3090401785714363 2201 24 1.761235119047619 2201 25 1.0741443452381105 2201 26 0.8695312500000014 2201 27 2.1168526785714334 2201 28 1.8887648809523796 2201 29 2.1261904761904873 2201 30 1.5431919642857252 2201 31 1.5082961309523881 2201 32 1.864025297619044 2201 33 1.474144345238102 2201 34 2.3780505952381006 2201 35 2.3258184523809575 2201 36 1.5672619047619136 2201 37 0.5941964285714434 2201 38 2.3274925595238116 2201 39 2.0810267857142932 2201 40 1.3713913690476218 2201 41 2.5897321428571587 2201 42 2.14962797619048 2201 43 3.207291666666684 2201 44 2.428980654761901 2201 45 1.9457589285714434 2201 46 1.752976190476197 2201 47 3.3438988095238145 2201 48 0.9616443452380992 2201 49 0.9208333333333343 2201 50 0.2603422619047677 2201 51 1.4147321428571473 1101 1 -0.316889880952381 1101 2 0.1235119047619122 1101 3 1.938467261904755 1101 4 1.3853794642857196 1101 5 -0.5816964285714192 1101 6 1.858891369047626 1101 7 0.5944940476190581 1101 8 0.38136160714287115 1101 9 0.6581473214285865 1101 10 0.41551339285714306 1101 11 -0.4888392857142847 1101 12 1.2894345238095397 1101 13 0.5584821428571374 1101 14 -1.9888764880952365 1101 15 0.14185267857142492 1101 16 1.1090773809523853 1101 17 0.9962797619047592 1101 18 -1.120610119047619 1101 19 0.8861607142857224 1101 20 0.8507068452381006 1101 21 0.3551711309523853 1101 22 1.232217261904772 1101 23 1.142373511904772 1101 24 1.5945684523809547 1101 25 1.4074776785714462 1101 26 -1.4638020833333343 1101 27 0.45018601190476915 1101 28 -0.4445684523809561 1101 29 1.2928571428571516 1101 30 1.876525297619061 1101 31 1.8416294642857238 1101 32 1.6973586309523796 1101 33 -0.8591889880952337 1101 34 0.21138392857143629 1101 35 2.1591517857142932 1101 36 1.9005952380952493 1101 37 2.427529761904779 1101 38 1.494159226190476 1101 39 2.414360119047629 1101 40 2.2047247023809575 1101 41 1.756398809523823 1101 42 1.8162946428571445 1101 43 3.207291666666684 1101 44 0.7623139880952365 1101 45 2.779092261904779 1101 46 2.419642857142861 1101 47 3.17723214285715 1101 48 3.294977678571435 1101 49 3.0874999999999986 1101 50 2.427008928571432 1101 51 3.748065476190483 2202 1 -0.316889880952381 2202 2 0.1235119047619122 2202 3 -2.061532738095245 2202 4 1.3853794642857196 2202 5 1.4183035714285808 2202 6 1.1922247023809618 2202 7 -0.7388392857142705 2202 8 -7.285305059523793 2202 9 0.6581473214285865 2202 10 -3.584486607142857 2202 11 0.1778273809523796 2202 12 -7.710565476190464 2202 13 1.2251488095238088 2202 14 0.6777901785714278 2202 15 -0.5248139880952394 2202 16 -6.557589285714279 2202 17 0.9962797619047592 2202 18 -0.4539434523809476 2202 19 1.5528273809523938 2202 20 1.517373511904772 2202 21 0.3551711309523853 2202 22 -1.4344494047618994 2202 23 -1.5242931547618994 2202 24 0.9279017857142833 2202 25 -1.2591889880952252 2202 26 1.5361979166666657 2202 27 -0.21648065476190226 2202 28 1.555431547619044 2202 29 -2.3738095238095127 2202 30 -7.123474702380943 2202 31 -2.491703869047612 2202 32 -2.635974702380956 2202 33 -9.192522321428566 2202 34 -6.788616071428567 2202 35 -6.84084821428571 2202 36 -2.4327380952380864 2202 37 -1.2391369047618923 2202 38 -0.5058407738095241 2202 39 -0.2523065476190425 2202 40 -0.4619419642857139 2202 41 -6.576934523809509 2202 42 1.8162946428571445 2202 43 2.54062500000002 2202 44 2.7623139880952365 2202 45 0.11242559523810769 2202 46 -7.913690476190471 2202 47 -1.1561011904761855 2202 48 -1.3716889880952365 2202 49 -13.579166666666666 2202 50 -4.9063244047619 2202 51 -13.585267857142853 1102 1 -0.316889880952381 1102 2 -1.8764880952380878 1102 3 -1.7281994047619094 1102 4 1.3853794642857196 1102 5 -0.5816964285714192 1102 6 -0.14110863095237391 1102 7 -1.405505952380942 1102 8 -0.28530505952379315 1102 9 -0.6751860119047493 1102 10 -1.584486607142857 1102 11 -1.8221726190476204 1102 12 0.6227678571428683 1102 13 -2.108184523809527 1102 14 0.6777901785714278 1102 15 0.14185267857142492 1102 16 -1.557589285714279 1102 17 -5.337053571428569 1102 18 0.21272321428571672 1102 19 0.2194940476190581 1102 20 0.18404017857143629 1102 21 0.3551711309523853 1102 22 0.5655505952381006 1102 23 0.4757068452381006 1102 24 0.9279017857142833 1102 25 -7.592522321428557 1102 26 -1.4638020833333343 1102 27 -0.8831473214285666 1102 28 -3.1112351190476204 1102 29 -1.3738095238095127 1102 30 -0.7901413690476105 1102 31 -9.15837053571428 1102 32 -3.3026413690476204 1102 33 0.474144345238102 1102 34 -4.121949404761899 1102 35 0.8258184523809575 1102 36 -7.099404761904754 1102 37 -1.2391369047618923 1102 38 -2.1725074404761884 1102 39 -1.9189732142857068 1102 40 -2.7952752976190425 1102 41 -6.576934523809509 1102 42 1.8162946428571445 1102 43 1.2072916666666842 1102 44 -0.5710193452380992 1102 45 1.4457589285714434 1102 46 -5.913690476190471 1102 47 -6.489434523809518 1102 48 -5.705022321428569 1102 49 -1.5791666666666657 1102 50 -0.23965773809523228 1102 51 2.4147321428571473 2203 1 -1.9835565476190453 2203 2 -1.543154761904752 2203 3 1.938467261904755 2203 4 -2.2812872023809447 2203 5 -2.2483630952380835 2203 6 -0.8077752976190382 2203 7 1.2611607142857295 2203 8 0.38136160714287115 2203 9 0.6581473214285865 2203 10 1.0821800595238145 2203 11 0.844494047619051 2203 12 1.2894345238095397 2203 13 -1.4415178571428626 2203 14 1.3444568452380992 2203 15 1.4751860119047606 2203 16 1.1090773809523853 2203 17 -0.3370535714285694 2203 18 1.5460565476190524 2203 19 -3.1138392857142776 2203 20 -1.1492931547618994 2203 21 1.0218377976190567 2203 22 -1.4344494047618994 2203 23 -0.857626488095228 2203 24 -2.738764880952381 2203 25 0.7408110119047748 2203 26 -0.4638020833333343 2203 27 1.7835193452380977 2203 28 -6.777901785714288 2203 29 -2.3738095238095127 2203 30 -7.123474702380943 2203 31 1.8416294642857238 2203 32 1.6973586309523796 2203 33 -0.1925223214285623 2203 34 1.5447172619047649 2203 35 1.4924851190476218 2203 36 -7.099404761904754 2203 37 -1.9058035714285566 2203 38 2.1608258928571473 2203 39 -5.918973214285707 2203 40 -0.4619419642857139 2203 41 2.4230654761904944 2203 42 -0.18370535714285552 2203 43 -5.792708333333316 2203 44 -6.237686011904767 2203 45 -1.5542410714285566 2203 46 1.0863095238095326 2203 47 -5.1561011904761855 2203 48 -0.37168898809523654 2203 49 1.0874999999999986 2203 50 0.427008928571432 2203 51 -5.2519345238095205 1103 1 0.18311011904761898 1103 2 2.123511904761912 1103 3 -1.061532738095245 1103 4 -0.11462053571428044 1103 5 1.4183035714285808 1103 6 -6.891108630952374 1103 7 -1.8221726190476062 1103 8 0.21469494047620685 1103 9 -0.17518601190474925 1103 10 0.24884672619047876 1103 11 0.0111607142857153 1103 12 0.956101190476204 1103 13 0.8918154761904731 1103 14 1.0111235119047635 1103 15 2.141852678571425 1103 16 1.942410714285721 1103 17 -4.920386904761905 1103 18 -1.5372767857142833 1103 19 -2.030505952380942 1103 20 -1.5659598214285637 1103 21 -2.061495535714279 1103 22 -4.851116071428564 1103 23 -1.9409598214285637 1103 24 -0.738764880952381 1103 25 -5.1758556547618895 1103 26 -5.380468749999999 1103 27 -1.6331473214285666 1103 28 -5.36123511904762 1103 29 0.6261904761904873 1103 30 1.0431919642857252 1103 31 -0.4917038690476119 1103 32 0.8640252976190439 1103 33 1.474144345238102 1103 34 1.8780505952381006 1103 35 1.8258184523809575 1103 36 -0.4327380952380864 1103 37 0.09419642857144339 1103 38 0.3274925595238116 1103 39 2.0810267857142932 1103 40 0.3713913690476218 1103 41 1.5897321428571587 1103 42 -4.60037202380952 1103 43 -4.542708333333316 1103 44 0.42898065476190084 1103 45 -4.304241071428557 1103 46 -3.497023809523803 1103 47 -3.4061011904761855 1103 48 -0.28835565476190084 1103 49 1.9208333333333343 1103 50 1.2603422619047677 1103 51 0.6647321428571473 2204 1 -0.316889880952381 2204 2 0.1235119047619122 2204 3 1.938467261904755 2204 4 1.3853794642857196 2204 5 1.4183035714285808 2204 6 1.858891369047626 2204 7 1.9278273809523938 2204 8 1.0480282738095426 2204 9 1.3248139880952507 2204 10 1.7488467261904788 2204 11 -1.155505952380949 2204 12 0.6227678571428683 2204 13 1.2251488095238088 2204 14 1.3444568452380992 2204 15 0.8085193452380892 2204 16 1.7757440476190567 2204 17 1.6629464285714306 2204 18 1.5460565476190524 2204 19 2.219494047619058 2204 20 2.1840401785714363 2204 21 1.688504464285721 2204 22 0.5655505952381006 2204 23 1.142373511904772 2204 24 1.5945684523809547 2204 25 1.4074776785714462 2204 26 1.5361979166666657 2204 27 1.7835193452380977 2204 28 1.555431547619044 2204 29 1.959523809523823 2204 30 1.876525297619061 2204 31 1.1749627976190524 2204 32 1.0306919642857082 2204 33 1.8074776785714377 2204 34 2.8780505952381006 2204 35 1.4924851190476218 2204 36 1.9005952380952493 2204 37 2.427529761904779 2204 38 2.1608258928571473 2204 39 1.7476934523809575 2204 40 0.20472470238095752 2204 41 2.4230654761904944 2204 42 0.4829613095238159 2204 43 2.54062500000002 2204 44 2.7623139880952365 2204 45 2.779092261904779 2204 46 3.0863095238095326 2204 47 3.8438988095238145 2204 48 3.294977678571435 2204 49 3.75416666666667 2204 50 3.7603422619047677 2204 51 4.414732142857147 2205 1 1.683110119047619 2205 2 0.1235119047619122 2205 3 -4.061532738095245 2205 4 -2.6146205357142804 2205 5 1.4183035714285808 2205 6 1.1922247023809618 2205 7 1.2611607142857295 2205 8 1.0480282738095426 2205 9 0.6581473214285865 2205 10 0.41551339285714306 2205 11 0.1778273809523796 2205 12 -0.04389880952379599 2205 13 0.5584821428571374 2205 14 0.011123511904763461 2205 15 -1.1914806547619108 2205 16 1.7757440476190567 2205 17 1.6629464285714306 2205 18 2.2127232142857167 2205 19 1.5528273809523938 2205 20 -1.1492931547618994 2205 21 0.3551711309523853 2205 22 1.232217261904772 2205 23 1.142373511904772 2205 24 1.5945684523809547 2205 25 -2.9258556547618895 2205 26 -1.4638020833333343 2205 27 2.450186011904769 2205 28 2.2220982142857153 2205 29 1.959523809523823 2205 30 1.876525297619061 2205 31 2.508296130952388 2205 32 1.6973586309523796 2205 33 1.8074776785714377 2205 34 2.2113839285714363 2205 35 2.1591517857142932 2205 36 2.5672619047619136 2205 37 3.0941964285714434 2205 38 2.1608258928571473 2205 39 1.7476934523809575 2205 40 2.2047247023809575 2205 41 2.4230654761904944 2205 42 2.482961309523816 2205 43 2.54062500000002 2205 44 2.7623139880952365 2205 45 2.779092261904779 2205 46 3.752976190476197 2205 47 3.17723214285715 2205 48 2.6283110119047635 2205 49 3.75416666666667 2205 50 2.427008928571432 2205 51 3.0813988095238116 1216 1 -0.316889880952381 1216 2 0.1235119047619122 1216 3 1.938467261904755 1216 4 1.3853794642857196 1216 5 -0.5816964285714192 1216 6 1.858891369047626 1216 7 1.2611607142857295 1216 8 1.7146949404762069 1216 9 1.3248139880952507 1216 10 1.0821800595238145 1216 11 0.844494047619051 1216 12 1.2894345238095397 1216 13 1.2251488095238088 1216 14 1.3444568452380992 1216 15 1.4751860119047606 1216 16 2.442410714285721 1216 17 2.329613095238095 1216 18 1.5460565476190524 1216 19 1.5528273809523938 1216 20 2.1840401785714363 1216 21 1.688504464285721 1216 22 1.232217261904772 1216 23 1.8090401785714363 1216 24 2.261235119047619 1216 25 2.0741443452381105 1216 26 2.202864583333337 1216 27 3.1168526785714334 1216 28 2.8887648809523796 1216 29 1.959523809523823 1216 30 1.876525297619061 1216 31 1.8416294642857238 1216 32 2.364025297619044 1216 33 2.474144345238102 1216 34 2.8780505952381006 1216 35 2.8258184523809575 1216 36 2.5672619047619136 1216 37 3.0941964285714434 1216 38 2.1608258928571473 1216 39 2.414360119047629 1216 40 2.2047247023809575 1216 41 2.4230654761904944 1216 42 2.482961309523816 1216 43 3.207291666666684 1216 44 3.428980654761901 1216 45 3.4457589285714434 1216 46 3.0863095238095326 1216 47 1.1772321428571502 1216 48 3.294977678571435 1216 49 3.75416666666667 1216 50 3.0936755952381034 1216 51 3.748065476190483 2206 1 1.683110119047619 2206 2 -0.2764880952380864 2206 3 -1.6615327380952465 2206 4 1.3853794642857196 2206 5 0.21830357142857792 2206 6 -1.7411086309523753 2206 7 1.1278273809523967 2206 8 0.514694940476204 2206 9 0.12481398809524791 2206 10 0.5488467261904759 2206 11 0.31116071428571246 2206 12 -1.6438988095237974 2206 13 -0.5081845238095255 2206 14 -0.3888764880952351 2206 15 0.9418526785714221 2206 16 -2.9575892857142776 2206 17 -3.870386904761908 2206 18 -1.1872767857142819 2206 19 1.419494047619061 2206 20 1.3840401785714391 2206 21 -0.311495535714279 2206 22 -1.7011160714285651 2206 23 1.0090401785714391 2206 24 0.6612351190476176 2206 25 0.4741443452381091 2206 26 -0.9304687499999957 2206 27 -9.683147321428567 2206 28 0.08876488095238244 2206 29 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1.3695312500000014 1211 27 2.1168526785714334 1211 28 2.3887648809523796 1211 29 2.1261904761904873 1211 30 2.0431919642857252 1211 31 1.5082961309523881 1211 32 1.364025297619044 1211 33 1.474144345238102 1211 34 1.8780505952381006 1211 35 1.8258184523809575 1211 36 1.5672619047619136 1211 37 2.0941964285714434 1211 38 1.8274925595238116 1211 39 2.0810267857142932 1211 40 1.3713913690476218 1211 41 2.0897321428571587 1211 42 2.14962797619048 1211 43 1.7072916666666842 1211 44 2.928980654761901 1211 45 2.9457589285714434 1211 46 3.752976190476197 1211 47 3.8438988095238145 1211 48 2.961644345238099 1211 49 -2.3291666666666657 1211 50 -3.4896577380952323 1211 51 0.6647321428571473 1208 1 -0.5168898809523839 1208 2 -4.076488095238087 1208 3 0.7384672619047521 1208 4 -2.214620535714282 1208 5 -3.5816964285714192 1208 6 -0.7411086309523753 1208 7 -3.4721726190476048 1208 8 -3.6853050595237917 1208 9 0.5248139880952536 1208 10 1.3488467261904802 1208 11 0.31116071428571246 1208 12 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1208 49 -7.579166666666666 1208 50 -5.239657738095232 1208 51 -0.5852678571428527 1209 1 -3.316889880952381 1209 2 -0.2764880952380864 1209 3 0.7384672619047521 1209 4 0.18537946428571672 1209 5 1.4183035714285808 1209 6 1.858891369047626 1209 7 1.5278273809523952 1209 8 0.9146949404762097 1209 9 0.5248139880952536 1209 10 -4.051153273809518 1209 11 -1.2888392857142819 1209 12 0.3561011904762026 1209 13 0.29181547619047166 1209 14 -0.3888764880952351 1209 15 -0.25814732142857366 1209 16 -0.3575892857142762 1209 17 0.7296130952380935 1209 18 1.0127232142857139 1209 19 -3.9805059523809447 1209 20 0.18404017857143629 1209 21 0.8885044642857238 1209 22 1.4988839285714377 1209 23 0.20904017857143486 1209 24 1.0612351190476161 1209 25 -0.7258556547618866 1209 26 1.26953125 1209 27 1.1168526785714334 1209 28 -0.511235119047619 1209 29 -0.7738095238095113 1209 30 -0.2568080357142719 1209 31 0.10829613095238955 1209 32 1.164025297619041 1209 33 -0.32585565476189515 1209 34 -0.521949404761898 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1.1840401785714363 1214 21 -1.6448288690476147 1214 22 -0.4344494047618994 1214 23 0.8090401785714363 1214 24 1.5945684523809547 1214 25 1.7408110119047748 1214 26 2.8695312500000014 1214 27 -1.7164806547619023 1214 28 0.3887648809523796 1214 29 0.1261904761904873 1214 30 -5.790141369047607 1214 31 -1.6583705357142762 1214 32 -1.8026413690476204 1214 33 -0.3591889880952337 1214 34 0.04471726190476488 1214 35 -2.3408482142857068 1214 36 -2.9327380952380864 1214 37 -1.072470238095221 1214 38 0.4941592261904759 1214 39 1.7476934523809575 1214 40 0.8713913690476218 1214 41 -2.4102678571428413 1214 42 -2.017038690476184 1214 43 -0.6260416666666515 1214 44 -2.7376860119047635 1214 45 -1.720907738095221 1214 46 -1.747023809523803 1214 47 -0.9894345238095212 1214 48 1.4616443452380992 1214 49 1.25416666666667 1214 50 -0.4063244047618966 1214 51 -2.9186011904761884 1215 1 0.18311011904761898 1215 2 2.123511904761912 1215 3 -2.311532738095245 1215 4 -0.11462053571428044 1215 5 -0.08169642857141923 1215 6 1.358891369047626 1215 7 -0.5721726190476062 1215 8 1.2146949404762069 1215 9 0.32481398809525075 1215 10 -5.501153273809521 1215 11 -2.2388392857142847 1215 12 -5.293898809523796 1215 13 -5.358184523809527 1215 14 -5.2388764880952365 1215 15 0.14185267857142492 1215 16 -0.057589285714279015 1215 17 -1.420386904761905 1215 18 -0.2872767857142833 1215 19 1.219494047619058 1215 20 -5.065959821428564 1215 21 0.18850446428572099 1215 22 -5.351116071428564 1215 23 -5.440959821428564 1215 24 0.261235119047619 1215 25 -5.6758556547618895 1215 26 -0.8804687499999986 1215 27 -0.13314732142856656 1215 28 1.8887648809523796 1215 29 1.6261904761904873 1215 30 0.04319196428572525 1215 31 1.5082961309523881 1215 32 0.3640252976190439 1215 33 -0.025855654761897995 1215 34 -4.371949404761899 1215 35 0.8258184523809575 1215 36 2.0672619047619136 1215 37 -0.6558035714285566 1215 38 -4.422507440476188 1215 39 1.5810267857142932 1215 40 -4.378608630952378 1215 41 -1.1602678571428413 1215 42 1.6496279761904802 1215 43 2.207291666666684 1215 44 -3.821019345238099 1215 45 2.4457589285714434 1215 46 -3.497023809523803 1215 47 -3.4061011904761855 1215 48 0.21164434523809916 1215 49 -2.3291666666666657 1215 50 -0.4896577380952323 1215 51 0.6647321428571473 1212 1 -0.316889880952381 1212 2 -6.20982142857142 1212 3 1.938467261904755 1212 4 0.38537946428571956 1212 5 -0.5816964285714192 1212 6 -3.9744419642857096 1212 7 -3.572172619047606 1212 8 -1.1186383928571288 1212 9 -1.1751860119047493 1212 10 1.0821800595238145 1212 11 0.5111607142857153 1212 12 0.956101190476204 1212 13 -0.9415178571428626 1212 14 -0.32220982142857224 1212 15 0.4751860119047606 1212 16 -0.2242559523809433 1212 17 -1.170386904761905 1212 18 0.879389880952381 1212 19 0.5528273809523938 1212 20 0.18404017857143629 1212 21 -3.1448288690476147 1212 22 -5.101116071428564 1212 23 -5.190959821428564 1212 24 -1.5720982142857167 1212 25 -0.25918898809522517 1212 26 -6.797135416666666 1212 27 -1.5498139880952309 1212 28 0.5554315476190439 1212 29 -1.2071428571428484 1212 30 -6.790141369047607 1212 31 -2.158370535714276 1212 32 -0.6359747023809561 1212 33 -4.025855654761898 1212 34 -5.121949404761899 1212 35 -6.507514880952375 1212 36 -2.9327380952380864 1212 37 -4.239136904761892 1212 38 -8.672507440476188 1212 39 -8.585639880952375 1212 40 -12.295275297619046 1212 41 -8.743601190476173 1212 42 -10.183705357142852 1212 43 -10.626041666666648 1212 44 -5.237686011904767 1212 45 -8.387574404761889 1212 46 -9.080357142857135 1212 47 -11.822767857142853 1212 48 -5.205022321428569 1212 49 -7.412499999999998 1212 50 -6.072991071428564 1212 51 -3.251934523809517 1213 1 -4.566889880952381 1213 2 2.123511904761912 1213 3 -1.061532738095245 1213 4 -3.1146205357142804 1213 5 -1.5816964285714192 1213 6 -2.891108630952374 1213 7 -0.07217261904760619 1213 8 -1.7853050595237931 1213 9 -2.1751860119047493 1213 10 -8.001153273809521 1213 11 -3.4888392857142847 1213 12 -4.543898809523796 1213 13 -4.358184523809527 1213 14 -6.2388764880952365 1213 15 -9.108147321428575 1213 16 -12.057589285714279 1213 17 -10.920386904761905 1213 18 -8.787276785714283 1213 19 -7.530505952380942 1213 20 -8.815959821428564 1213 21 -6.061495535714279 1213 22 -9.351116071428564 1213 23 -0.19095982142856371 1213 24 -3.488764880952381 1213 25 -2.6758556547618895 1213 26 -9.630468749999999 1213 27 -17.633147321428567 1213 28 -2.8612351190476204 1213 29 -9.373809523809513 1213 30 -9.956808035714275 1213 31 -3.741703869047612 1213 32 -7.385974702380956 1213 33 -7.775855654761898 1213 34 -13.1219494047619 1213 35 -6.9241815476190425 1213 36 -3.6827380952380864 1213 37 -0.4058035714285566 1213 38 -0.6725074404761884 1213 39 2.0810267857142932 1213 40 -1.3786086309523782 1213 41 0.08973214285715869 1213 42 -8.10037202380952 1213 43 -1.5427083333333158 1213 44 -2.321019345238099 1213 45 -3.0542410714285566 1213 46 -12.247023809523803 1213 47 -2.4061011904761855 1213 48 -13.5383556547619 1213 49 -9.329166666666666 1213 50 -9.989657738095232 1213 51 -16.335267857142853 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 0.0 20 1.0 21 2.0 22 2.0 23 6.0 24 6.0 25 19.0 26 19.0 27 27.0 28 32.0 29 51.0 30 60.0 31 62.0 32 74.0 33 99.0 34 117.0 35 145.0 36 155.0 37 231.0 38 380.0 39 1121.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.494446572194562 14.93680582152432 12.409038682497126 53.15970892378399 2 5.553427805438529 15.128303332056683 65.95174262734585 13.366526235158943 3 9.268479509766372 21.677518192263502 61.164304864036765 7.8896974339333585 4 5.630026809651475 56.98965913443125 23.094599770202986 14.285714285714285 5 7.085407889697434 24.01378782075833 60.66641133665263 8.234392952891612 6 12.677135197242437 64.72615855993872 8.923783990808118 13.672922252010725 7 8.04289544235925 28.5331290693221 11.949444657219455 51.474530831099194 8 10.647261585599386 64.49636154729988 11.71964764458062 13.136729222520108 9 10.800459594025277 18.498659517426276 12.792033703561852 57.9088471849866 10 6.932209881271543 61.20260436614324 17.27307545001915 14.592110302566066 11 11.681348142474148 19.226350057449253 53.50440444274225 15.587897357334354 12 12.945231711987745 17.847567981616237 17.809268479509765 51.39793182688624 13 12.255840674071237 14.668709306779013 59.78552278820375 13.289927230945997 14 13.213328226733054 17.27307545001915 15.549597855227882 53.963998468019916 15 16.698582918422062 15.93259287629261 15.511298353121408 51.857525852163924 16 50.59364228265033 21.715817694369974 17.196476445806205 10.494063577173497 17 12.14094216775182 63.155878973573344 14.93680582152432 9.766373037150517 18 49.48295672156262 16.775181922635003 21.06472615855994 12.677135197242437 19 9.421677518192263 18.15396399846802 16.0857908847185 56.33856759862121 20 6.702412868632708 18.72845653006511 17.57947146687093 56.98965913443125 21 7.3535044044427424 22.941401761777097 17.809268479509765 51.8958253542704 22 5.974722328609728 61.011106855610876 18.498659517426276 14.51551129835312 23 13.32822673305247 16.39218690157028 19.26464955955573 51.01493680582152 24 50.019149751053234 20.33703561853696 19.992340099578705 9.651474530831099 25 11.221754117196477 62.42818843355037 19.26464955955573 7.085407889697434 26 52.35541937954806 20.37533512064343 20.681731137495213 6.58751436231329 27 6.58751436231329 63.50057449253159 21.026426656453467 8.885484488701646 28 9.689774032937573 17.31137495212562 23.362696284948296 49.636154729988505 29 7.162006893910379 20.873228648027574 64.6112600536193 7.3535044044427424 30 6.242818843355036 59.977020298736115 23.477594791267713 10.302566066641134 31 7.200306396016852 17.426273458445042 25.04787437763309 50.32554576790502 32 6.58751436231329 18.00076599004213 67.29222520107238 8.119494446572196 33 10.149368058215243 16.890080428954423 65.22405208732287 7.736499425507469 34 47.45308310991957 17.426273458445042 23.70739180390655 11.413251627728838 35 7.200306396016852 19.954040597472233 23.86058981233244 48.98506319417847 36 6.2811183454615085 60.360015319800844 23.97548831865186 9.383378016085791 37 6.549214860206817 58.67483722711605 27.307545001914974 7.46840291076216 38 6.2811183454615085 15.741095365760247 65.76024511681348 12.217541171964765 39 7.506702412868632 16.468785905783225 26.196859440827268 49.82765224052088 40 5.9364228265032555 15.81769436997319 67.25392569896592 10.991957104557642 41 9.07698199923401 17.464572960551514 22.596706242818843 50.86173879739564 42 49.13826120260437 15.358100344695519 28.226733052470315 7.276905400229797 43 6.779011872845652 19.26464955955573 64.95595557257757 9.000382995021065 44 5.744925315970892 60.05361930294906 26.388356951359633 7.813098429720414 45 8.693986978169283 17.57947146687093 64.87935656836461 8.847184986595174 46 49.02336269628495 16.353887399463808 21.945614707008808 12.677135197242437 47 6.357717349674455 16.23898889314439 23.28609728073535 54.117196476445805 48 8.19609345078514 16.39218690157028 22.443508234392954 52.96821141325163 49 48.142474147836076 16.85178092684795 25.201072386058982 9.80467253925699 50 4.5193412485637685 18.15396399846802 64.7644580620452 12.562236690923017 51 5.361930294906166 16.58368441210264 25.354270394484875 52.70011489850632 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 131.0 1 71.5 2 12.0 3 14.5 4 17.0 5 19.0 6 21.0 7 17.0 8 13.0 9 16.0 10 19.0 11 41.5 12 64.0 13 56.0 14 48.0 15 57.5 16 67.0 17 54.5 18 42.0 19 39.0 20 36.0 21 34.5 22 33.0 23 23.0 24 13.0 25 17.5 26 20.0 27 18.0 28 18.0 29 18.0 30 44.0 31 70.0 32 51.5 33 33.0 34 32.0 35 31.0 36 36.0 37 41.0 38 48.5 39 56.0 40 54.5 41 53.0 42 70.0 43 87.0 44 82.0 45 77.0 46 71.5 47 66.0 48 137.0 49 208.0 50 688.5 51 1169.0 52 612.5 53 56.0 54 43.0 55 30.0 56 22.5 57 15.0 58 12.5 59 10.0 60 11.5 61 13.0 62 9.5 63 6.0 64 6.0 65 6.0 66 4.5 67 3.0 68 2.5 69 2.0 70 2.5 71 3.0 72 1.5 73 0.0 74 0.0 75 0.5 76 1.0 77 1.0 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 2611.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.11643048640368 #Duplication Level Percentage of deduplicated Percentage of total 1 90.98621420996818 32.860972807353505 2 3.2873806998939554 2.3745691306013024 3 1.4846235418875928 1.6085790884718498 4 0.8483563096500532 1.2255840674071237 5 0.4241781548250266 0.7659900421294523 6 0.8483563096500532 1.8383761011106856 7 0.4241781548250266 1.0723860589812333 8 0.10604453870625664 0.3063960168517809 9 0.10604453870625664 0.3446955189582535 >10 1.0604453870625663 7.62160091918805 >50 0.2120890774125133 5.0555342780543855 >100 0.10604453870625664 4.672539256989659 >500 0.0 0.0 >1k 0.10604453870625664 40.25277671390272 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC 1051 40.25277671390272 TruSeq Adapter, Index 12 (95% over 21bp) AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 122 4.672539256989659 No Hit TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT 80 3.0639601685178093 TruSeq Adapter, Index 12 (95% over 22bp) GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT 52 1.9915741095365762 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 40 1.5319800842589046 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26 0.9957870547682881 No Hit ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC 23 0.8808885484488702 TruSeq Adapter, Index 12 (95% over 24bp) GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19 0.7276905400229797 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18 0.689391037916507 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17 0.6510915358100344 No Hit TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT 16 0.6127920337035618 No Hit TATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTT 15 0.5744925315970892 RNA PCR Primer, Index 12 (95% over 23bp) CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13 0.49789352738414405 No Hit GCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTT 12 0.4595940252776714 No Hit CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC 9 0.3446955189582535 No Hit ACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACT 8 0.3063960168517809 No Hit AGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7 0.2680965147453083 No Hit CTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT 7 0.2680965147453083 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7 0.2680965147453083 No Hit CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC 7 0.2680965147453083 RNA PCR Primer, Index 27 (95% over 21bp) TGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTT 6 0.2297970126388357 No Hit ACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTCTG 6 0.2297970126388357 RNA PCR Primer, Index 12 (96% over 26bp) CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC 6 0.2297970126388357 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.2297970126388357 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6 0.2297970126388357 No Hit GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACAT 6 0.2297970126388357 No Hit AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCCTGTCTCTTATACAC 6 0.2297970126388357 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC 6 0.2297970126388357 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.19149751053236308 No Hit CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG 5 0.19149751053236308 No Hit CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC 5 0.19149751053236308 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5 0.19149751053236308 No Hit TTTTTCGCGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4 0.15319800842589046 No Hit CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC 4 0.15319800842589046 No Hit GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4 0.15319800842589046 No Hit CTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT 4 0.15319800842589046 No Hit TTTTTTGCTCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4 0.15319800842589046 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4 0.15319800842589046 No Hit CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC 4 0.15319800842589046 No Hit AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCCTGTCTCTTATACACAT 4 0.15319800842589046 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.11489850631941785 No Hit CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC 3 0.11489850631941785 No Hit CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3 0.11489850631941785 No Hit GCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT 3 0.11489850631941785 No Hit CGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.11489850631941785 No Hit CTTATACACATCTACGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC 3 0.11489850631941785 TruSeq Adapter, Index 12 (95% over 21bp) CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.11489850631941785 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC 3 0.11489850631941785 No Hit GAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.11489850631941785 No Hit GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACAC 3 0.11489850631941785 No Hit TGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT 3 0.11489850631941785 No Hit TTTTTTTGCTCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3 0.11489850631941785 No Hit GATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT 3 0.11489850631941785 No Hit CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC 3 0.11489850631941785 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.038299502106472615 18 0.0 0.0 0.15319800842589046 19 0.0 0.0 0.15319800842589046 20 0.0 0.0 0.15319800842589046 21 0.0 0.0 0.19149751053236308 22 0.0 0.0 0.2297970126388357 23 0.0 0.0 0.3063960168517809 24 0.0 0.0 0.34469551895825357 25 0.0 0.0 0.42129452317119875 26 0.0 0.0 0.4595940252776714 27 0.0 0.0 0.5361930294906166 28 0.0 0.0 0.6510915358100344 29 0.0 0.0 0.8808885484488702 30 0.0 0.0 1.1872845653006512 31 0.0 0.0 1.493680582152432 32 0.0 0.0 1.991574109536576 33 0.0 0.0 2.2596706242818843 34 0.0 0.0 2.795863653772501 35 0.0 0.0 3.4086556874760627 36 0.0 0.0 3.791650708540789 37 0.0 0.0 4.787437763309077 38 0.0 0.0 5.208732286480275 39 0.0 0.0 5.821524320183838 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACATCT 110 0.0 42.954544 7 TTGCAAT 105 0.0 42.85714 32 TGCAATC 105 0.0 42.85714 33 TGCCGTC 105 0.0 42.85714 45 ACTTGCA 105 0.0 42.85714 30 CAATCTC 105 0.0 42.85714 35 GCAATCT 105 0.0 42.85714 34 TCTCCGA 105 0.0 42.85714 11 CGAGACT 105 0.0 42.85714 23 AGACTAC 105 0.0 42.85714 25 TCCGAGC 105 0.0 42.85714 13 TACTTGC 105 0.0 42.85714 29 CTACTTG 105 0.0 42.85714 28 GAGACTA 105 0.0 42.85714 24 ACTACTT 105 0.0 42.85714 27 CTCCGAG 105 0.0 42.85714 12 GACTACT 105 0.0 42.85714 26 CATCTCC 115 0.0 41.086956 9 ACATCTC 115 0.0 41.086956 8 TACACAT 115 0.0 41.086956 5 >>END_MODULE