H23NVBCXX l02n01 36h_9.34100000005bf7.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	H23NVBCXX l02n01 36h_9.34100000005bf7.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2611
Sequences flagged as poor quality	0
Sequence length	51
%GC	40

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	1051	40.25277671390272	TruSeq Adapter, Index 12 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	122	4.672539256989659	No Hit
TTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCT	80	3.0639601685178093	TruSeq Adapter, Index 12 (95% over 22bp)
GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTT	52	1.9915741095365762	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40	1.5319800842589046	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26	0.9957870547682881	No Hit
ATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTC	23	0.8808885484488702	TruSeq Adapter, Index 12 (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19	0.7276905400229797	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18	0.689391037916507	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17	0.6510915358100344	No Hit
TCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGT	16	0.6127920337035618	No Hit
TATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTT	15	0.5744925315970892	RNA PCR Primer, Index 12 (95% over 23bp)
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13	0.49789352738414405	No Hit
GCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTT	12	0.4595940252776714	No Hit
CTTATACACATCTCCGAGCCCACGAGACTTGCTGGAATCTCGTATGCCGTC	9	0.3446955189582535	No Hit
ACTCTGCGTTGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACT	8	0.3063960168517809	No Hit
AGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7	0.2680965147453083	No Hit
CTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTT	7	0.2680965147453083	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7	0.2680965147453083	No Hit
CTTATACACATCTCCGAGCCCACGAGACGAATTGCTATCTCGTATGCCGTC	7	0.2680965147453083	RNA PCR Primer, Index 27 (95% over 21bp)
TGATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTT	6	0.2297970126388357	No Hit
ACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTCTTCTG	6	0.2297970126388357	RNA PCR Primer, Index 12 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACTGCGACCTATCTCGTATGCCGTC	6	0.2297970126388357	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.2297970126388357	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6	0.2297970126388357	No Hit
GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTCTGTCTCTTATACACAT	6	0.2297970126388357	No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTGCAAGCCTGTCTCTTATACAC	6	0.2297970126388357	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGATCCTATCTCGTATGCCGTC	6	0.2297970126388357	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.19149751053236308	No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTACTTGCAATCTCGTATGCCG	5	0.19149751053236308	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATAACCTATCTCGTATGCCGTC	5	0.19149751053236308	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5	0.19149751053236308	No Hit
TTTTTCGCGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.15319800842589046	No Hit
CTTATACACATCTCCGAGCCCACGAGACAGTGTCTTATCTCGTATGCCGTC	4	0.15319800842589046	No Hit
GCGTTGATACCACTGCTTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.15319800842589046	No Hit
CTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTT	4	0.15319800842589046	No Hit
TTTTTTGCTCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4	0.15319800842589046	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4	0.15319800842589046	No Hit
CTTATACACATCTCCGAGCCCACGAGACGTCTTAGTATCTCGTATGCCGTC	4	0.15319800842589046	No Hit
AAAGTACTCTGCGTTGATACCACTGCTTGCAAGCCTGTCTCTTATACACAT	4	0.15319800842589046	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.11489850631941785	No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCGTGTATCTCGTATGCCGTC	3	0.11489850631941785	No Hit
CGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3	0.11489850631941785	No Hit
GCTTAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTT	3	0.11489850631941785	No Hit
CGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.11489850631941785	No Hit
CTTATACACATCTACGAGCCCACGAGACTACTTGCAATCTCGTATGCCGTC	3	0.11489850631941785	TruSeq Adapter, Index 12 (95% over 21bp)
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.11489850631941785	No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTATATCTCGTATGCCGTC	3	0.11489850631941785	No Hit
GAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.11489850631941785	No Hit
GCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTATACAC	3	0.11489850631941785	No Hit
TGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	3	0.11489850631941785	No Hit
TTTTTTTGCTCGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3	0.11489850631941785	No Hit
GATACCACTGCTTGCAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTT	3	0.11489850631941785	No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGCGTTATCTCGTATGCCGTC	3	0.11489850631941785	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
CACATCT	110	42.954544	7
TTGCAAT	105	42.85714	32
TGCAATC	105	42.85714	33
TGCCGTC	105	42.85714	45
ACTTGCA	105	42.85714	30
CAATCTC	105	42.85714	35
GCAATCT	105	42.85714	34
TCTCCGA	105	42.85714	11
CGAGACT	105	42.85714	23
AGACTAC	105	42.85714	25
TCCGAGC	105	42.85714	13
TACTTGC	105	42.85714	29
CTACTTG	105	42.85714	28
GAGACTA	105	42.85714	24
ACTACTT	105	42.85714	27
CTCCGAG	105	42.85714	12
GACTACT	105	42.85714	26
CATCTCC	115	41.086956	9
ACATCTC	115	41.086956	8
TACACAT	115	41.086956	5

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content