FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_88.34100000006183.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_88.34100000006183.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences315384
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC13300.4217081399183218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG2000.039.3748618
TATGCCG2000.039.3748643
TATCTCG1950.038.0767936
ACGAGAC2100.037.49986622
ATAATAT1750.037.28558332
CGAGACA1800.036.24987423
TCGTATG2300.036.19552240
CCCACGA2200.035.79532619
TCTCGTA2200.035.79532638
CGAGCCC2200.035.79532615
CCACGAG2200.035.79532620
CTCCGAG2300.035.21726612
CCGAGCC2300.035.21726614
ATCTCGT2250.034.99987837
ATCTCCG2250.034.99987810
CGTATGC2350.034.46796441
ATATCTC1900.034.34198835
TCCGAGC2300.034.2390113
CACGAGA2300.034.2390121
ATGCCGT2300.034.2390144
Produced by FastQC (version 0.11.2)