FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_87.34100000006176.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_87.34100000006176.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106426
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC62095.834100689681093No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT2380.22362956420423583RNA PCR Primer, Index 44 (95% over 22bp)
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA2070.19450134365662525No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT1370.1287279424200853No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT1190.11181478210211791No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGAA207.004792E-444.999535
TGCCGTC6650.042.63113445
TCTCGTA6800.042.0216238
ATCTCGT6900.041.73869337
CTCGTAT6900.041.73869339
TATGCCG6850.041.71489343
TCGTATG6850.041.71489340
CACGAGA6950.041.43841621
ATGCCGT6850.041.3864344
AACATAA6700.041.3055429
ACGAGAC6950.041.1146822
CCACGAG6950.041.1146820
TATCTCG6850.041.05796436
TAATATC6750.040.9995733
ATAATAT6750.040.9995732
CATAATA6750.040.9995731
CGTATGC7000.040.82100341
CCCACGA7000.040.82100319
ATATCTC6850.040.729535
ACAACAT6800.040.69810527