FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_86.34100000006169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_86.34100000006169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences249067
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC16970.6813427712222012No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA3430.13771394845563642No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT2580.10358658513572652No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1600.033.74954643
TGCCGTC1600.033.74954645
TCTCGTA1700.033.0877938
CCACGAG1700.033.0877920
TATCTCG1700.031.76427836
GTATGCC1700.031.76427842
CGTATGC1800.031.2495841
CCCACGA1800.031.2495819
ATGCCGT1750.030.85672844
TCGTATG1900.030.7890640
CTCGTAT1850.030.40499739
ACGAGAC1850.030.40499722
AGCCTAT300.00513994629.99966
ATAATAT1650.029.99959832
CGAGACA1800.029.99959823
ATCTCGT1900.029.60486637
GCCCACG1900.029.60486618
CACGAGA1900.029.60486621
CGAGCCC1900.029.60486615
CATAATA1750.028.28533431
Produced by FastQC (version 0.11.2)