FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_85.3410000000615d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_85.3410000000615d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186717
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC33521.7952302147099621No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT3010.1612065318101726No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA2750.14728171510896168No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT2390.1280011996765158RNA PCR Primer, Index 44 (95% over 22bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4250.041.29362545
ACGAGAC4300.040.8134722
TATGCCG4350.040.3443543
ATGCCGT4350.040.3443544
TATCTCG4150.040.12000736
CGAGACA4150.040.12000723
TCGTATG4400.039.88588740
CGTATGC4450.039.4377341
TCTCGTA4350.039.3098838
CACGAGA4500.038.99953521
CCACGAG4500.038.99953520
CTCGTAT4450.038.93211739
GCCCACG4600.038.1517218
CCCACGA4600.038.1517219
GTATGCC4600.038.1517242
TAATATC4250.038.11719533
ATCTCGT4500.037.99954637
CATAATA4400.037.84045831
CAACATA4500.037.49955428
AGACAAC4450.037.41528325