FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_84.34100000006140.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_84.34100000006140.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115105
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC39953.4707441032101127No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT3740.3249207245558403RNA PCR Primer, Index 44 (95% over 22bp)
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCAT2150.18678597802006863No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1870.16246036227792016No Hit
TCTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGT1290.11207158681204117No Hit
AAAAAGTACTCTGCGTTGATACCACCTGTCTCTTATACACATCTCCGAGCC1220.10599018287650405No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4450.040.44943645
ATGCCGT4650.039.1935544
TATGCCG4700.038.77659643
TATCTCG4600.038.64130836
GCCCACG4800.038.43749618
CACGAGA4800.038.43749621
ACGAGAC4800.038.43749622
ATAATAT4750.038.3684232
CATAATA4750.038.3684231
CTCGTAT4650.038.22580739
CAACATA4850.038.04123328
CCCACGA4850.038.04123319
CGTATGC4800.037.96874641
AATATCT4700.037.8191534
TCTCGTA4700.037.8191538
GTATGCC4850.037.57731642
ATCTCGT4750.037.4210537
ACAACAT4950.037.27272827
CCACGAG4950.037.27272820
TAATATC4850.037.113433