FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_83.34100000006133.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_83.34100000006133.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151755
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC21691.4292774537906494No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT2230.14694738229382887RNA PCR Primer, Index 44 (95% over 22bp)
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAA1630.10740997001746236No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2500.036.8991945
CCCACGA2600.036.34535619
GCCCACG2650.035.65959518
ACGAGAC2650.035.65959522
CCACGAG2650.035.65959520
ATCTCGT2700.034.99923337
CACGAGA2700.034.99923321
TCGTATG2700.034.99923340
CTCGTAT2650.034.8105639
TATGCCG2650.034.8105643
GTATGCC2650.034.8105642
ATAATAT2600.034.61462832
CGAGACA2600.034.61462823
ATATCTC2700.034.1659235
TATCTCG2700.034.1659236
TCTCGTA2700.034.1659238
ATGCCGT2650.033.96151744
CGTATGC2750.033.5447241
GACAACA2700.033.33260326
CATAATA2800.032.94570531
Produced by FastQC (version 0.11.2)