FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_82.34100000006126.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_82.34100000006126.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254743
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC10900.42788221854967556No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4160.1633018375382248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACG1450.035.68825518
TATCTCG1400.035.35575536
CGTATGC1500.034.49864641
TATGCCG1550.033.38578443
ACGAGAC1550.033.38578422
ATGCCGT1600.032.3424844
CACGAGA1650.031.36240421
CCCACGA1650.031.36240419
TCTCGTA1650.031.36240438
TCGTATG1650.031.36240440
TGCCGTC1650.031.36240445
CTCGTAT1700.030.4399839
GTATGCC1700.030.4399842
GCCTAAG300.005130948530.0106031
CCGAGCC1750.029.57607314
CGAGCCC1750.029.57607315
CCACGAG1750.029.57026520
TCCGAGC1800.028.75451713
CGAGACA1650.028.63523923
TCCTATA406.1542785E-428.1238962
Produced by FastQC (version 0.11.2)