FastQCFastQC Report
Thu 9 Apr 2015
H23NVBCXX l02n01 36h_81.3410000000610c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH23NVBCXX l02n01 36h_81.3410000000610c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50412
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14062.7890184876616675No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5521.0949773863365866No Hit
CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC5271.0453860192017774No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3030.6010473696738872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2410.4780607791795604No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1560.30945013092120927No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT750.1487741014044275No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.10711735301118781No Hit
TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT540.10711735301118781RNA PCR Primer, Index 44 (95% over 22bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAT750.045.00000432
CACGAGA800.045.021
TATCTCG800.045.036
ACGAGAC800.045.022
CCACGAG800.045.020
TGCCGTC800.045.045
GCCCACG850.042.3529418
CTCGTAT850.042.3529439
CTCCGAG850.042.3529412
TCCGAGC850.042.3529413
TATGCCG850.042.3529443
CCCACGA850.042.3529419
ATATCTC850.042.3529435
TCTCGTA850.042.3529438
CCGAGCC850.042.3529414
CGAGCCC850.042.3529415
TCTCCGA850.042.3529411
ATGCCGT850.042.3529444
TAATATC800.042.187533
CAACATA800.042.187528