##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename H23NVBCXX l02n01 36h_81.3410000000610c.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 50412 Sequences flagged as poor quality 0 Sequence length 51 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.34727842577164 38.0 38.0 38.0 32.0 38.0 2 36.36320717289534 38.0 38.0 38.0 32.0 38.0 3 36.336407998095694 38.0 38.0 38.0 32.0 38.0 4 36.415734348964534 38.0 38.0 38.0 32.0 38.0 5 36.47139569943664 38.0 38.0 38.0 32.0 38.0 6 38.37407760057129 40.0 38.0 40.0 38.0 40.0 7 38.27116559549314 40.0 38.0 40.0 38.0 40.0 8 38.226969769102595 40.0 38.0 40.0 38.0 40.0 9 38.26838847893359 40.0 38.0 40.0 38.0 40.0 10 38.180492739823855 40.0 38.0 40.0 38.0 40.0 11 38.154169642148695 40.0 38.0 40.0 38.0 40.0 12 38.1000952154249 40.0 38.0 40.0 38.0 40.0 13 38.097814012536695 40.0 38.0 40.0 38.0 40.0 14 38.02178052844561 40.0 38.0 40.0 32.0 40.0 15 38.07363326192176 40.0 38.0 40.0 38.0 40.0 16 38.07061810679997 40.0 38.0 40.0 32.0 40.0 17 38.055800206300084 40.0 38.0 40.0 38.0 40.0 18 38.10477664048243 40.0 38.0 40.0 38.0 40.0 19 38.162461318733634 40.0 38.0 40.0 38.0 40.0 20 38.14796080298342 40.0 38.0 40.0 38.0 40.0 21 38.14690946600016 40.0 38.0 40.0 38.0 40.0 22 38.14450924383083 40.0 38.0 40.0 38.0 40.0 23 38.15276124732207 40.0 38.0 40.0 38.0 40.0 24 38.130703007220504 40.0 38.0 40.0 38.0 40.0 25 38.12508926446084 40.0 38.0 40.0 38.0 40.0 26 38.07385146393716 40.0 38.0 40.0 38.0 40.0 27 38.01858684440213 40.0 38.0 40.0 32.0 40.0 28 37.99291835277315 40.0 38.0 40.0 32.0 40.0 29 37.969273188923275 40.0 38.0 40.0 32.0 40.0 30 37.930373720542725 40.0 38.0 40.0 32.0 40.0 31 37.89946838054431 40.0 38.0 40.0 32.0 40.0 32 37.9211298897088 40.0 38.0 40.0 32.0 40.0 33 37.88302388320241 40.0 38.0 40.0 32.0 40.0 34 37.86126319130366 40.0 38.0 40.0 32.0 40.0 35 37.838768547171306 40.0 38.0 40.0 32.0 40.0 36 37.76711893993494 40.0 38.0 40.0 32.0 40.0 37 37.78338490835515 40.0 38.0 40.0 32.0 40.0 38 37.737998889153374 40.0 38.0 40.0 32.0 40.0 39 37.69836546853924 40.0 38.0 40.0 32.0 40.0 40 37.64798063953027 40.0 38.0 40.0 32.0 40.0 41 37.6529199396969 40.0 38.0 40.0 32.0 40.0 42 37.557605332063794 40.0 38.0 40.0 32.0 40.0 43 37.49049829405697 40.0 38.0 40.0 32.0 40.0 44 37.42057446639689 40.0 38.0 40.0 32.0 40.0 45 37.35838689200984 40.0 38.0 40.0 32.0 40.0 46 37.21334206141395 40.0 38.0 40.0 32.0 40.0 47 37.168114734587 40.0 38.0 40.0 32.0 40.0 48 37.08341267952075 40.0 38.0 40.0 32.0 40.0 49 37.02912005078156 40.0 38.0 40.0 32.0 40.0 50 36.952908037768786 40.0 38.0 40.0 32.0 40.0 51 36.5854558438467 38.0 38.0 40.0 32.0 40.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2201 1 0.38926801173459324 2201 2 0.29106375499134174 2201 3 0.05867253095632208 2201 4 0.7301351842201669 2201 5 0.13142950536139608 2201 6 0.368554570520665 2201 7 0.03701077923156504 2201 8 0.10812007110690303 2201 9 0.5168532853820693 2201 10 0.30541898844405324 2201 11 0.35821586170742137 2201 12 0.0611688335905356 2201 13 -0.5010163867001083 2201 14 0.19552042346333565 2201 15 0.1910018301827776 2201 16 -0.07938431269875679 2201 17 0.20199283919006916 2201 18 0.497902227627975 2201 19 0.8379391839560526 2201 20 0.5461040839558393 2201 21 0.4094212446460972 2201 22 0.013415273942321448 2201 23 -0.1924701367203312 2201 24 -0.4480867420283161 2201 25 0.5751905823787951 2201 26 0.17674134060522562 2201 27 0.29023165239601667 2201 28 0.25641255341258073 2201 29 0.4411949204311796 2201 30 -0.39236025594001944 2201 31 0.6046638945233838 2201 32 1.0997652644692195 2201 33 0.846851080214492 2201 34 1.138002750822075 2201 35 0.7999448981061121 2201 36 0.411165329755498 2201 37 0.519874365865796 2201 38 0.37586637320308114 2201 39 0.6082356816767174 2201 40 0.3688200445486487 2201 41 0.24480027318859499 2201 42 0.633151850332986 2201 43 0.10217067617401199 2201 44 0.3454308685942138 2201 45 0.4917498457898333 2201 46 0.43958050020171413 2201 47 -0.32438654683962653 2201 48 -0.08811206996032439 2201 49 -0.304060778691678 2201 50 0.5087854176971902 2201 51 -0.28049589776389183 1101 1 0.7617954842620662 1101 2 0.6405143044418935 1101 3 0.7938373661211529 1101 4 0.18947584356082103 1101 5 0.5523086262405172 1101 6 0.8894336913997876 1101 7 0.5392085814293708 1101 8 0.48394424693108107 1101 9 0.35311702164580794 1101 10 0.7032211862462532 1101 11 0.4197543232458827 1101 12 0.9402897127114116 1101 13 0.5945880089042888 1101 14 0.4746413025842102 1101 15 0.6239688631498126 1101 16 0.6931431598287148 1101 17 0.27232250951973924 1101 18 0.1473527770785239 1101 19 -0.13568718967032112 1101 20 0.707642545494302 1101 21 -0.7784908432659918 1101 22 0.7002284607555112 1101 23 0.08005733580714747 1101 24 -0.6986361925777658 1101 25 -0.02480941762119926 1101 26 -0.5375443736804897 1101 27 0.43638549854986053 1101 28 0.4465224435224755 1101 29 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0.41672624466915664 2202 15 0.7444321836131351 2202 16 0.15491922160477856 2202 17 0.1244460616432903 2202 18 0.10102073074648388 2202 19 0.4558186018354675 2202 20 0.7671020049537631 2202 21 0.31919255441740546 2202 22 -0.1576865971595467 2202 23 0.7012928570426666 2202 24 -0.2503736443152178 2202 25 -0.17075536356714593 2202 26 0.27674134060522704 2202 27 0.35530441746877983 2202 28 0.06659966359968905 2202 29 0.5025254817617366 2202 30 0.521153257573495 2202 31 -0.41051282065333083 2202 32 0.4376031023070581 2202 33 0.7206556540190618 2202 34 -0.17467916185984222 2202 35 0.7772837754449853 2202 36 -0.8293752107850452 2202 37 0.6967974427888706 2202 38 0.5602738576105608 2202 39 0.05210262554366096 2202 40 0.16091983664843923 2202 41 0.26995619834451645 2202 42 0.6693264865076216 2202 43 0.6996758736792117 2202 44 1.5188196419829865 2202 45 0.3757415297815214 2202 46 1.0119297725509924 2202 47 0.63320181074873 2202 48 0.35076526891700865 2202 49 -0.4936657682966654 2202 50 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49 -0.013441641731077425 1212 50 -1.822359047593615 1212 51 -1.0808711322854663 1213 1 -0.29644627397969003 1213 2 -0.33970547577788324 1213 3 -0.053415381131593165 1213 4 -0.8127219586369776 1213 5 -0.3125265385946463 1213 6 -0.28639048442438764 1213 7 0.250197592418381 1213 8 0.29713106011789137 1213 9 -0.06776209923331322 1213 10 -0.6660095829845218 1213 11 0.05052355401511477 1213 12 -0.18718281476111542 1213 13 -0.2504669361506586 1213 14 -0.4374466095037022 1213 15 -0.48811904893810265 1213 16 -1.0881755214899655 1213 17 -1.2903148531176285 1213 18 -0.4185812888555418 1213 19 -0.14887400285713426 1213 20 -0.7198299819782221 1213 21 0.2292014644263176 1213 22 -0.1272440667170187 1213 23 -0.583678927929121 1213 24 0.031034137092568415 1213 25 -0.1478863406981219 1213 26 -0.9265553626914738 1213 27 -0.294383732219373 1213 28 -0.07105997405994202 1213 29 -0.8225413433050832 1213 30 -0.10225036583013036 1213 31 -0.4810503911909052 1213 32 -1.1200149553109995 1213 33 -0.2652368318734233 1213 34 -0.39386538104606217 1213 35 -0.5231320249708133 1213 36 0.255121373711539 1213 37 -0.8032025572111294 1213 38 -0.8417160443793392 1213 39 -0.7983577249166842 1213 40 -0.6751359994073951 1213 41 -0.2760788476905276 1213 42 -0.3075074903263513 1213 43 -0.706620532617201 1213 44 -0.5512724281090797 1213 45 -1.2093490553090618 1213 46 -0.7714085107872961 1213 47 -0.7024085248616103 1213 48 -0.9606395424877974 1213 49 -0.8447201193510168 1213 50 -0.7109948020830288 1213 51 -0.7991772164452158 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 2.0 17 1.0 18 1.0 19 3.0 20 7.0 21 11.0 22 26.0 23 52.0 24 102.0 25 149.0 26 185.0 27 297.0 28 408.0 29 598.0 30 726.0 31 925.0 32 1127.0 33 1344.0 34 1598.0 35 2203.0 36 2821.0 37 4308.0 38 7663.0 39 25853.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.40716638228642 21.741200746002143 13.737550097218366 25.114082774493074 2 14.971235865899624 23.26125768696687 38.60146796270581 23.16603848442769 3 18.85662143933984 26.130683170673645 29.300563357930653 25.712132032055862 4 12.91359200190431 20.489169245417756 34.575101166388954 32.02213758628898 5 13.726890422915178 37.754899627072916 33.33134967864794 15.186860271363962 6 30.79822264540189 39.308101245735145 15.74823454733 14.145441561532968 7 26.180274537808458 34.88653495199556 19.50130921209236 19.431881298103626 8 23.992303419820676 37.56446877727525 19.051019598508294 19.39220820439578 9 23.661033087360153 20.183686423867332 20.534793303181782 35.62048718559073 10 15.41299690549869 32.21455209077204 29.99682615250337 22.375624851225897 11 31.50440371340157 26.767436324684603 23.230580020630008 18.49757994128382 12 22.597794175989844 27.761247322066176 28.465444735380462 21.175513766563515 13 26.15448702689836 25.172577957629137 26.32309767515671 22.349837340315798 14 20.673649131159248 24.960326906292153 27.20384035547092 27.16218360707768 15 21.621836070776798 31.381417122907244 24.85717686265175 22.139569943664206 16 21.455634682906506 31.574457955604927 25.930848425938784 21.039058935549782 17 19.751249702451798 31.079901610727607 28.044909942077282 21.123938744743313 18 20.49394961317199 29.275937314024997 30.081333068835548 20.148780003967467 19 20.078552725541538 29.17956042212172 30.516543680076175 20.225343172260573 20 19.949615170991034 28.868126636515118 30.48083789573911 20.70142029675474 21 19.49337459335079 28.842339125605015 30.837895739109733 20.82639054193446 22 19.935729588193286 29.669523129413633 30.339998413076252 20.05474886931683 23 19.765135285249542 28.517019757200668 30.41537729112116 21.30246766642863 24 21.15567721970959 28.61818614615568 30.492739823851462 19.733396810283267 25 20.066650797429183 29.52669999206538 30.435213837975084 19.97143537253035 26 20.772831865428866 28.725303499166866 30.861699595334446 19.640165040069824 27 19.515194794890107 30.088074268031423 30.490756169166072 19.9059747679124 28 19.77108624930572 28.897881456796 30.207093549154962 21.123938744743313 29 20.13211140204713 30.222962786638103 30.1198127429977 19.52511306831707 30 20.233679157326772 29.699867092499655 30.144214556346828 19.922239193826744 31 20.00515750218202 28.584464016504008 30.34594937713243 21.064429104181546 32 19.538998651114813 29.64373561850353 30.401491708323412 20.41577402205824 33 19.886138221058477 28.705466952312943 31.51828929619932 19.890105530429263 34 20.493136554788542 29.78457510116639 30.21304451321114 19.509243830833928 35 19.993255574069664 30.006744425930332 30.33008013964929 19.66991986035071 36 19.92977862413711 28.96334206141395 31.147345870030946 19.959533444417996 37 19.965484408474175 29.0803776878521 30.4054590176942 20.54867888597953 38 19.469570737126084 28.326588907403 31.31992382766008 20.88391652781084 39 20.2967547409347 28.219471554391813 30.302308974053798 21.18146473061969 40 20.503054828215504 27.69578671744823 31.657145124176783 20.144013330159485 41 20.169800841069588 28.179798460683962 30.19519162104261 21.45520907720384 42 21.00095215424899 28.374196619852416 30.332063794334687 20.292787431563912 43 19.842497817979847 28.029040704594145 31.08386892009839 21.044592557327622 44 19.465603427755298 29.048639212885817 30.3796715067841 21.106085852574783 45 19.72942950091248 28.104419582639057 31.256446877727523 20.90970403872094 46 21.038641593271443 27.755296358009996 29.96112036816631 21.244941680552248 47 19.54136993910059 28.541389776040944 29.6284541072385 22.288786177619965 48 19.564786162024912 28.390065857335557 29.629850035705786 22.415297944933744 49 20.61612314528287 28.552725541537725 29.489010553042927 21.342140760136473 50 19.146235023407126 28.673728477346668 30.651432198682855 21.52860430056336 51 19.182321318759797 28.380710559203347 29.13054690444546 23.3064212175914 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1500.0 1 803.5 2 107.0 3 110.5 4 114.0 5 116.5 6 119.0 7 96.0 8 73.0 9 95.5 10 118.0 11 218.5 12 319.0 13 537.0 14 755.0 15 848.0 16 941.0 17 791.5 18 642.0 19 535.5 20 429.0 21 408.5 22 388.0 23 377.5 24 367.0 25 397.0 26 542.0 27 657.0 28 790.5 29 924.0 30 1121.0 31 1318.0 32 1544.5 33 1771.0 34 1997.0 35 2223.0 36 2401.5 37 2580.0 38 2666.5 39 2753.0 40 2809.0 41 2865.0 42 2959.5 43 3054.0 44 3173.5 45 3293.0 46 3557.5 47 3822.0 48 3521.5 49 3221.0 50 3173.5 51 3126.0 52 2973.0 53 2820.0 54 2610.0 55 2400.0 56 2198.5 57 1997.0 58 1831.0 59 1665.0 60 1387.0 61 1109.0 62 1012.5 63 916.0 64 744.5 65 573.0 66 510.5 67 448.0 68 343.5 69 239.0 70 186.0 71 133.0 72 117.5 73 102.0 74 83.0 75 44.0 76 24.0 77 16.0 78 8.0 79 6.5 80 5.0 81 3.5 82 2.0 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019836546853923668 2 0.003967309370784734 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.001983654685392367 17 0.0 18 0.003967309370784734 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.001983654685392367 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.001983654685392367 48 0.0 49 0.0 50 0.0 51 0.001983654685392367 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 50412.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.6592118915955 #Duplication Level Percentage of deduplicated Percentage of total 1 95.43618046489388 83.6586036549668 2 3.88884363499018 6.817859364257734 3 0.3699467768970901 0.9728772871390448 4 0.11055696891160743 0.38765347055660526 5 0.03690695546189022 0.1617617314553756 6 0.0399271522657325 0.20999896204139057 7 0.01845992321566592 0.11327276244652493 8 0.03185832238453627 0.22341403459334597 9 0.005524415779937318 0.04358393400877193 >10 0.04144199660089686 0.7342893226958441 >50 0.006784570334705017 0.36278439206001745 >100 0.0067844091319373585 1.3876705678634484 >500 0.004522939421291372 2.1389950610351662 >1k 0.002261469710645686 2.7872354548799296 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1406 2.7890184876616675 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 552 1.0949773863365866 No Hit CTTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTC 527 1.0453860192017774 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 303 0.6010473696738872 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 241 0.4780607791795604 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 156 0.30945013092120927 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 75 0.1487741014044275 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 54 0.10711735301118781 No Hit TTATACACATCTCCGAGCCCACGAGACAACATAATATCTCGTATGCCGTCT 54 0.10711735301118781 RNA PCR Primer, Index 44 (95% over 22bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.0 0.0 0.0 2 0.0 0.0 0.0 3 0.0 0.0 0.0 4 0.0 0.0 0.0 5 0.0 0.0 0.0 6 0.0 0.0 0.0 7 0.0 0.0 0.0 8 0.0 0.0 0.0 9 0.0 0.0 0.0 10 0.0 0.0 0.0 11 0.0 0.0 0.0 12 0.0 0.0 0.0 13 0.0 0.0 0.0 14 0.0 0.0 0.0 15 0.0 0.0 0.0 16 0.0 0.0 0.0 17 0.0 0.0 0.0 18 0.0 0.0 0.0 19 0.0 0.0 0.0 20 0.0 0.0 0.0 21 0.0 0.0 0.001983654685392367 22 0.0 0.0 0.001983654685392367 23 0.0 0.0 0.009918273426961834 24 0.0 0.0 0.015869237483138936 25 0.0 0.0 0.019836546853923668 26 0.0 0.0 0.02578751091010077 27 0.0 0.0 0.027771165595493138 28 0.0 0.0 0.039673093707847336 29 0.0 0.0 0.0654606046179481 30 0.0 0.0 0.12298659049432675 31 0.0 0.0 0.24200587161786877 32 0.0 0.0 0.4185511386177894 33 0.0 0.0 0.5812108228199635 34 0.0 0.0 0.827184003808617 35 0.0 0.0 1.0731571847972705 36 0.0 0.0 1.3647544235499485 37 0.0 0.0 1.7912401809093073 38 0.0 0.0 2.267317305403475 39 0.0 0.0 2.785051178290883 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAATAT 75 0.0 45.000004 32 CACGAGA 80 0.0 45.0 21 TATCTCG 80 0.0 45.0 36 ACGAGAC 80 0.0 45.0 22 CCACGAG 80 0.0 45.0 20 TGCCGTC 80 0.0 45.0 45 GCCCACG 85 0.0 42.35294 18 CTCGTAT 85 0.0 42.35294 39 CTCCGAG 85 0.0 42.35294 12 TCCGAGC 85 0.0 42.35294 13 TATGCCG 85 0.0 42.35294 43 CCCACGA 85 0.0 42.35294 19 ATATCTC 85 0.0 42.35294 35 TCTCGTA 85 0.0 42.35294 38 CCGAGCC 85 0.0 42.35294 14 CGAGCCC 85 0.0 42.35294 15 TCTCCGA 85 0.0 42.35294 11 ATGCCGT 85 0.0 42.35294 44 TAATATC 80 0.0 42.1875 33 CAACATA 80 0.0 42.1875 28 >>END_MODULE